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Peptide record

TPDB20338

Nigrocin-M1 Antibacterial Anticancer Antifungal Toxicity standard

21 amino acids

3D PDB MODEL

Drag to rotate. Click a residue or atom to inspect it; the selected residue is highlighted in amber.

TPDB ID TPDB20338

Peptide_Name

Nigrocin-M1

Activity

Antibacterial Anticancer Antifungal Toxicity

Activity_category_statistics

Anti-infective peptides Cancer-related peptides Toxicity and safety peptides

Database_Source

Peptipedia CancerPPD 2.0

Sequence_Type

standard

Has_Modification No

Description A 21-aa standard natural multi-activity (Antibacterial, Anticancer, Antifungal, and other sources) peptide sequence curated from Peptipedia and CancerPPD 2.0, with an available 3D structural model.

Sequence

GLLGKILGAGKKVLLGVSGLL

FASTA

>TPDB20338|Antibacterial;Anticancer;Antifungal;Toxicity|length=21
GLLGKILGAGKKVLLGVSGLL

Formula C94H172N24O23

Absent Amino Acids RNDCEQHMFPTWY

Common Amino Acids L

Mass 2006.55

PI 10.84

Basic Residues 3

Acidic Residues 0

Hydrophobic Residues 11

Net Charge +3

Boman Index -1.10

Hydrophobicity 1.257

Aliphatic Index 180.95

Half Life

Mammalian: 30 hour Yeast: >20 hour E.coli: >10 hour

Extinction Coefficient Cystines 0

Absorbance 280nm 0.00

Polar Residues 1

number

Amino Acid Distribution

A: 1 R: 0 N: 0 D: 0 C: 0 E: 0 Q: 0 G: 6 H: 0 I: 1 L: 7 K: 3 M: 0 F: 0 P: 0 S: 1 T: 0 W: 0 Y: 0 V: 2

Length

Formula

C94H172N24O23

Mass

2006.55

10.84

Net Charge

Boman Index

-1.10

Hydrophobicity

1.257

C_terminal_Modification

Free

Chirality

Linear_or_Cyclic

Linear

N_terminal_Modification

Free

Nature

Antimicrobial and Antitumor

Origin

Synthetic Analogue of Nigrocin M1

Evidence 1 Activity

Activity

Activity Endpoint MIC

Detail Source Activity Antibacterial

Activity Measurement 4 µM

Target

Target Organism S. constellatus B003 VISIT

Source & Reference

Database Source NA

Other

Evidence Text

C 12,493 or B038 V1S1A or B042 V2E1A (MIC 4-16 uM), E.faecalis NCTC 12,697 (MIC >512 uM), S. constellatus B003 VISIT (MIC 4 uM), Gram- E. coli NCTC 10,418 (MIC 8 uM), K. pneumoniae ATCC 13,883 or ATCC 43,816 (MIC 8-16

Evidence 2 Activity

Activity

Activity Endpoint MIC

Detail Source Activity Antibacterial

Activity Measurement 32 µM

Target

Target Organism Candida albicans NYCY 1467

Source & Reference

Database Source NA

Other

Evidence Text

8-16 uM), P. aeruginosa ATCC 27,853 or ATCC 9097 or B004 V2S2B (MIC >256 uM), and fungus C. albicans NYCY 1467 (MIC 32 uM). Anticancer: H157 (IC50 24 uM), PC-3 IC50 31.27 uM0, U-251MG (IC50 25.06 uM), and MCF-7 (

Evidence 3 Activity

Activity

Activity Endpoint IC50

Activity Raw

IC50 = 24 µM

Detail Source Activity Anticancer

Activity Measurement 24 µM

Target

Cancer Type Non-small cell lung cancer

Cell Line H-157

Tissue Affected Lung

Assay & Model

Assay MTT assay

Testing Time 24-h

Source & Reference

Database Source CancerPPD 2.0

Source CancerPPD2

Year 2022

Other

Source Activity Label	Source Definition
Anticancer	CancerPPD 2.0 manually curated experimentally verified anticancer peptide/protein annotation.

Evidence 4 Activity

Activity

Activity Endpoint IC50

Activity Raw

IC50 = 31.27 µM

Detail Source Activity Anticancer

Activity Measurement 31.27 µM

Target

Cancer Type Prostate cancer

Cell Line PC-3

Tissue Affected Prostate

Assay & Model

Assay MTT assay

Testing Time 24-h

Source & Reference

Database Source CancerPPD 2.0

Source CancerPPD2

Year 2022

Evidence 5 Activity

Activity

Activity Endpoint IC50

Activity Raw

IC50 = 25.06 µM

Detail Source Activity Anticancer

Activity Measurement 25.06 µM

Target

Cancer Type Brain Tumor

Cell Line U-251-MG

Tissue Affected Brain

Assay & Model

Assay MTT assay

Testing Time 24-h

Source & Reference

Database Source CancerPPD 2.0

Source CancerPPD2

Year 2022

Evidence 6 Activity

Activity

Activity Endpoint IC50

Activity Raw

IC50 = 24.60 µM

Detail Source Activity Anticancer

Activity Measurement 24.60 µM

Target

Cancer Type Breast Cancer

Cell Line MCF-7

Tissue Affected Breast

Assay & Model

Assay MTT assay

Testing Time 24-h

Source & Reference

Database Source CancerPPD 2.0

Source CancerPPD2

Year 2022

Evidence 7 Activity

Activity

Activity Endpoint MIC

Detail Source Activity Antifungal

Activity Measurement 4 µM

Target

Target Organism S. constellatus B003 VISIT

Source & Reference

Database Source NA

Other

Evidence Text

Evidence 8 Activity

Activity

Activity Endpoint MIC

Detail Source Activity Antifungal

Activity Measurement 32 µM

Target

Target Organism Candida albicans NYCY 1467

Source & Reference

Database Source NA

Other

Evidence Text

Evidence 9 Activity

Activity

Activity Endpoint 18% Hemolysis

Detail Source Activity Toxicity

Activity Measurement 100 µM

Toxicity Type Hemolytic Cytotoxic

Toxicity Raw

18% Hemolysis | 100 | µM | target cell: Horse erythrocytes

Target

Target Cell Horse erythrocytes

Source & Reference

Database Source NA

Source Database DBAASP

Reference

A novel antimicrobial peptide found in Pelophylax nigromaculatus | J Genet Eng Biotechnol | 2022

Evidence 10 Activity

Activity

Activity Endpoint LC50

Detail Source Activity Toxicity

Activity Measurement 30 µM

Toxicity Type Cytotoxic

Toxicity Raw

LC50 | 30 | µM | target cell: Human keratinocytes HaCat

Target

Target Cell Human keratinocytes HaCat

Source & Reference

Database Source NA

Source Database DBAASP

Reference

A novel antimicrobial peptide found in Pelophylax nigromaculatus | J Genet Eng Biotechnol | 2022

Evidence 11 Activity

Activity

Activity Endpoint LC50

Detail Source Activity Toxicity

Activity Measurement 60 µM

Toxicity Type Cytotoxic

Toxicity Raw

LC50 | 60 | µM | target cell: Human microvascular endothelial cells HMEC-1

Target

Target Cell Human microvascular endothelial cells HMEC-1

Source & Reference

Database Source NA

Source Database DBAASP

Reference

A novel antimicrobial peptide found in Pelophylax nigromaculatus | J Genet Eng Biotechnol | 2022

Evidence 12 Activity

Activity

Detail Source Activity Toxicity

Toxicity Type Hemolytic Cytotoxic

Toxicity Raw

Hemolytic_activity: Not available | Cytotoxicity: Not available

Target

Target Cell erythrocytes

Source & Reference

Database Source NA

Source Database DRAMP

Reference

J Genet Eng Biotechnol. 2022 May 23 20(1):77.