TPDB

3D PDB MODEL

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TPDB ID TPDB20112

Activity

Antibacterial Toxicity

Activity_category_statistics

Anti-infective peptides Toxicity and safety peptides

Database_Source

Peptipedia

Sequence_Type

standard

Has_Modification No

Description A 30-aa standard natural multi-activity (Antibacterial and Toxicity) peptide sequence curated from Peptipedia, with an available 3D structural model.

Sequence

GILRLVTRRFRFSPTNLNRYTVARLVSGVP

FASTA

>TPDB20112|Antibacterial;Toxicity|length=30
GILRLVTRRFRFSPTNLNRYTVARLVSGVP

Formula C156H260N50O39

Absent Amino Acids DCEQHKMW

Common Amino Acids R

Mass 3460.09

PI 12.70

Basic Residues 6

Acidic Residues 0

Hydrophobic Residues 13

Net Charge +6

Boman Index 1.66

Hydrophobicity 0.030

Aliphatic Index 107.00

Half Life

Mammalian: 30 hour Yeast: >20 hour E.coli: >10 hour

Extinction Coefficient Cystines 1490

Absorbance 280nm 43.06

Polar Residues 8

number

1

A

6

R

2

N

0

D

0

C

0

E

0

Q

2

G

0

H

1

I

4

L

0

K

0

M

2

F

2

P

2

S

3

T

0

W

1

Y

4

V

Amino Acid Distribution

A: 1 R: 6 N: 2 D: 0 C: 0 E: 0 Q: 0 G: 2 H: 0 I: 1 L: 4 K: 0 M: 0 F: 2 P: 2 S: 2 T: 3 W: 0 Y: 1 V: 4

Length

30

Formula

C156H260N50O39

Mass

3460.09

PI

12.70

Net Charge

+6

Boman Index

1.66

Hydrophobicity

0.030

Evidence 1 Activity

Activity

Activity Endpoint MIC

Detail Source Activity Antibacterial

Activity Measurement 32 µg/mL

Target

Target Organism Escherichia coli ATCC 25922

Source & Reference

Database Source NA

Other

Evidence Text

active against E. coli ATCC 25922 (MIC 32 ug/ml), S. enteritidis (MIC 64 ug/ml), and S. aureus ATCC 29213 (MIC 64-128 ug/ml).

Evidence 2 Activity

Activity

Activity Endpoint MIC

Detail Source Activity Antibacterial

Activity Measurement 64 µg/mL

Target

Target Organism S. enteritidis

Source & Reference

Database Source NA

Other

Evidence Text

active against E. coli ATCC 25922 (MIC 32 ug/ml), S. enteritidis (MIC 64 ug/ml), and S. aureus ATCC 29213 (MIC 64-128 ug/ml).

Evidence 3 Activity

Activity

Activity Endpoint MIC

Detail Source Activity Antibacterial

Activity Measurement 64-128 µg/mL

Target

Target Organism Staphylococcus aureus ATCC 29213

Source & Reference

Database Source NA

Other

Evidence Text

active against E. coli ATCC 25922 (MIC 32 ug/ml), S. enteritidis (MIC 64 ug/ml), and S. aureus ATCC 29213 (MIC 64-128 ug/ml).

Evidence 4 Activity

Activity

Activity Endpoint 50% Hemolysis

Detail Source Activity Toxicity

Activity Measurement >128 µg/mL

Toxicity Type Hemolytic Cytotoxic

Toxicity Raw

50% Hemolysis | >128 | µg/mL | target cell: Pig erythrocytes

Target

Target Cell Pig erythrocytes

Source & Reference

Database Source NA

Source Database DBAASP

Reference

Mining Amphibian and Insect Transcriptomes for Antimicrobial Peptide Sequences with rAMPage. | Antibiotics (Basel) | 2022