TPDB

3D PDB MODEL

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TPDB ID TPDB19743

Activity

Antibacterial Anticancer Toxicity

Activity_category_statistics

Anti-infective peptides Cancer-related peptides Toxicity and safety peptides

Database_Source

Peptipedia

Sequence_Type

standard

Has_Modification No

Description A 36-aa standard natural multi-activity (Antibacterial, Anticancer, and Toxicity) peptide sequence curated from Peptipedia, with an available 3D structural model.

Sequence

GFGCPWNAYECDRHCVSKGYTGGNCRGKIRQTCHCY

FASTA

>TPDB19743|Antibacterial;Anticancer;Toxicity|length=36
GFGCPWNAYECDRHCVSKGYTGGNCRGKIRQTCHCY

Formula C171H253N55O50S6

Absent Amino Acids LM

Common Amino Acids CG

Mass 4071.59

PI 8.30

Basic Residues 7

Acidic Residues 2

Hydrophobic Residues 8

Net Charge +3

Boman Index 4.27

Hydrophobicity -0.775

Aliphatic Index 21.67

Half Life

Mammalian: 30 hour Yeast: >20 hour E.coli: >10 hour

Extinction Coefficient Cystines 10345

Absorbance 280nm 254.08

Polar Residues 15

number

1

A

3

R

2

N

1

D

6

C

1

E

1

Q

6

G

2

H

1

I

0

L

2

K

0

M

1

F

1

P

1

S

2

T

1

W

3

Y

1

V

Amino Acid Distribution

A: 1 R: 3 N: 2 D: 1 C: 6 E: 1 Q: 1 G: 6 H: 2 I: 1 L: 0 K: 2 M: 0 F: 1 P: 1 S: 1 T: 2 W: 1 Y: 3 V: 1

Length

36

Formula

C171H253N55O50S6

Mass

4071.59

PI

8.30

Net Charge

+3

Boman Index

4.27

Hydrophobicity

-0.775

Evidence 1 Activity

Activity

Detail Source Activity Antibacterial

Source & Reference

Database Source NA

Other

Evidence Text

(lethal concentration LC: B. megaterium CGMCC 1.0459 (CL 0.038-0.11 uM), B. subtilis CGMCC 1.2428 (LC 1 uM), S. aureus CGMCC 1.89 (LC 5.2 uM), penicillin-sensitive S. epidermidis P1111 or P1389 (LC 5.2 or 1.14), penicillin-resistant S. aureus P1383 (LC 4.28 uM), MRSA P1374 or P 1386 (LC 0.62 uM or 0.74 uM), Methicillin-resistant coagulase negative Staphylococci P1369 (LC 1.07 uM), S. aureus J685, J698, J700, J706, J708 and J710 (LC 1.14-10.4 uM), S. pneumoniae D39, R6, ST556, ST556 (StrR), TIGR4 (LC 0.14 uM), S. mutans CGMCC 1.2499 (ATCC 25175) (LC 0.381.01 uM), S. salivarius CGMCC 1.2498 (ATCC 7073) (LC 0.49 uM), S. sanguinis CGMCC 1.2497 (ATCC 49295) (LC 1.41 uM), S. griseus NBRC 13350 (LC 2.89 uM), S. scabiei CGMCC 4.1765 (ATCC 49173) (LC 2.01 uM), L. fusiformis (LC 0.27 uM), E. faecalis V583 (ATCC 700802) (4.65 uM).

Evidence 2 Activity

Activity

Activity Endpoint 5% Hemolysis

Detail Source Activity Toxicity

Activity Measurement 25 µM

Toxicity Type Hemolytic Cytotoxic

Toxicity Raw

5% Hemolysis | 25 | µM | target cell: Human erythrocytes

Target

Target Cell Human erythrocytes

Source & Reference

Database Source NA

Source Database DBAASP

Reference

Adaptively evolved human oral actinomyces-sourced defensins show therapeutic potential. | EMBO Mol Med | 2022

Evidence 3 Activity

Activity

Activity Endpoint 12% Killing

Detail Source Activity Toxicity

Activity Measurement 25 µM

Toxicity Type Cytotoxic

Toxicity Raw

12% Killing | 25 | µM | target cell: Human embryonic kidney HEK293 cells

Target

Target Cell Human embryonic kidney HEK293 cells

Source & Reference

Database Source NA

Source Database DBAASP

Reference

Adaptively evolved human oral actinomyces-sourced defensins show therapeutic potential. | EMBO Mol Med | 2022

Evidence 4 Activity

Activity

Detail Source Activity Toxicity

Toxicity Type Hemolytic Cytotoxic

Toxicity Raw

Hemolytic_activity: Not available | Cytotoxicity: Not available

Target

Target Cell erythrocytes

Source & Reference

Database Source NA

Source Database DRAMP

Reference

EMBO Mol Med. 2022 Feb 7 14(2):e14499.