TPDB

3D PDB MODEL

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TPDB ID TPDB18745

Activity

Antibacterial Toxicity

Activity_category_statistics

Anti-infective peptides Toxicity and safety peptides

Database_Source

Peptipedia

Sequence_Type

standard

Has_Modification No

Description A 28-aa standard natural multi-activity (Antibacterial and Toxicity) peptide sequence curated from Peptipedia, with an available 3D structural model.

Sequence

EEQRFLPVVAGLAAKVLPSIICAVTKKC

FASTA

>TPDB18745|Antibacterial;Toxicity|length=28
EEQRFLPVVAGLAAKVLPSIICAVTKKC

Formula C135H231N35O36S2

Absent Amino Acids NDHMWY

Common Amino Acids AV

Mass 2984.65

PI 9.08

Basic Residues 4

Acidic Residues 2

Hydrophobic Residues 14

Net Charge +2

Boman Index 5.27

Hydrophobicity 0.729

Aliphatic Index 125.36

Half Life

Mammalian: 1 hour Yeast: 30 min E.coli: >10 hour

Extinction Coefficient Cystines 125

Absorbance 280nm 4.19

Polar Residues 5

number

4

A

1

R

0

N

0

D

2

C

2

E

1

Q

1

G

0

H

2

I

3

L

3

K

0

M

1

F

2

P

1

S

1

T

0

W

0

Y

4

V

Amino Acid Distribution

A: 4 R: 1 N: 0 D: 0 C: 2 E: 2 Q: 1 G: 1 H: 0 I: 2 L: 3 K: 3 M: 0 F: 1 P: 2 S: 1 T: 1 W: 0 Y: 0 V: 4

Length

28

Formula

C135H231N35O36S2

Mass

2984.65

PI

9.08

Net Charge

+2

Boman Index

5.27

Hydrophobicity

0.729

Evidence 1 Activity

Activity

Activity Endpoint MIC

Detail Source Activity Antibacterial

Activity Measurement 64 µg/mL

Target

Target Organism Escherichia coli ATCC 25922

Source & Reference

Database Source NA

Other

Evidence Text

Active against E. coli ATCC 25922 (MIC 64 ug/ml), S.enteritidis (MIC >128 ug/ml), and S. aureus ATCC 29213 (MIC 64-128 ug/ml).

Evidence 2 Activity

Activity

Activity Endpoint MIC

Detail Source Activity Antibacterial

Activity Measurement >128 µg/mL

Target

Target Organism S.enteritidis

Source & Reference

Database Source NA

Other

Evidence Text

Active against E. coli ATCC 25922 (MIC 64 ug/ml), S.enteritidis (MIC >128 ug/ml), and S. aureus ATCC 29213 (MIC 64-128 ug/ml).

Evidence 3 Activity

Activity

Activity Endpoint MIC

Detail Source Activity Antibacterial

Activity Measurement 64-128 µg/mL

Target

Target Organism Staphylococcus aureus ATCC 29213

Source & Reference

Database Source NA

Other

Evidence Text

Active against E. coli ATCC 25922 (MIC 64 ug/ml), S.enteritidis (MIC >128 ug/ml), and S. aureus ATCC 29213 (MIC 64-128 ug/ml).

Evidence 4 Activity

Activity

Activity Endpoint 50% Hemolysis

Detail Source Activity Toxicity

Activity Measurement >128 µg/mL

Toxicity Type Hemolytic Cytotoxic

Toxicity Raw

50% Hemolysis | >128 | µg/mL | target cell: Pig erythrocytes

Target

Target Cell Pig erythrocytes

Source & Reference

Database Source NA

Source Database DBAASP

Reference

Associating Biological Activity and Predicted Structure of Antimicrobial Peptides from Amphibians and Insects. | Antibiotics (Basel) | 2022