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Peptide record

TPDB18456

CREKA Antibacterial Anticancer Blood-Brain Barrier Cell-penetrating Peptides Toxicity Tumor-homing Peptides standard
5 amino acids
Basic Information
3D PDB MODEL
Drag to rotate. Click a residue or atom to inspect it; the selected residue is highlighted in amber.
TPDB18456
CREKA
Antibacterial Anticancer Blood-Brain Barrier Cell-penetrating Peptides Toxicity Tumor-homing Peptides
Anti-infective peptides Cancer-related peptides Toxicity and safety peptides Delivery and barrier-penetrating peptides
Peptipedia BBPpredict CPPsite3.0 StackTHP StackTHPred TumorHoPe2.0
standard
No
A 5-aa standard natural multi-activity (Antibacterial, Anticancer, Blood-Brain Barrier, and other sources) peptide sequence curated from Peptipedia, BBPpredict, CPPsite3.0, and other sources, with an available 3D structural model.
Sequence
CREKA
Physicochemical Analysis
C23H43N9O8S1
NDQGHILMFPSTWYV
ARCEK
605.71
8.97
2
1
1
+1
3.41
-1.520
20.00
Mammalian: 1.2 hour Yeast: >20 hour E.coli: >10 hour
0
0.00
1
Residue Composition
number
1
A
1
R
0
N
0
D
1
C
1
E
0
Q
0
G
0
H
0
I
0
L
1
K
0
M
0
F
0
P
0
S
0
T
0
W
0
Y
0
V
Amino Acid Distribution
A: 1 R: 1 N: 0 D: 0 C: 1 E: 1 Q: 0 G: 0 H: 0 I: 0 L: 0 K: 1 M: 0 F: 0 P: 0 S: 0 T: 0 W: 0 Y: 0 V: 0
Chemical Descriptors
5
C23H43N9O8S1
605.71
8.97
+1
3.41
-1.520
Evidence Records 20 records
Evidence 1 Activity

Activity

IC50
Toxicity
>200 µM
Cytotoxic
IC50 | >200 | µM | target cell: Mouse fibroblasts NIH 3T3

Target

Mouse fibroblasts NIH 3T3

Source & Reference

NA
DBAASP
Identification of BP16 as a non-toxic cell-penetrating peptide with highly efficient drug delivery properties. | Org Biomol Chem | 2014
Evidence 2 Activity

Activity

Tumor-homing Peptides

Target

Tumor vessel and stroma of tumors
Homing
Breast cancer ,Fibrosarcoma

Source & Reference

TumorHoPe2.0
TumorHoPe2
Systematic surface engineering of magnetic nanoworms for in vivo tumor targeting
2009
Park JH et al. Small. 2009 Mar 5(6):694-700.
US20050048063A1

Other

Source Activity Label Source Definition
Tumor-homing Peptides
TumorHoPe2.0 experimentally validated tumor-homing peptide annotation.
Mice bearing human (MDA-MB-435 human carcinoma and HT1080 human fibrosarcoma) xenograft
Phage display library
Superparamagnetic nanospheres, superparamagnetic IO nanoworms (NWs)
Evidence 3 Activity

Activity

Tumor-homing Peptides

Target

Tumor cell
Targeting
Breast cancer, Ovarian cancer

Source & Reference

TumorHoPe2.0
TumorHoPe2
Compositions for treating cancer and methods for making the same
2019
US 10300143 B2
US 10300143 B2

Other

4T1 breast cancer cell line, MDA-MB-468 breast cancer cell line, 4306-ovarian cancer cell line, Lewis lung carcinoma (LLC) cells, hepatocellular carcinoma cells (CP20)
BALB/c mice
Synthetic
diacylglycerols, dialkylglycerols
Evidence 4 Activity

Activity

Tumor-homing Peptides

Target

Tumor cell, Tumor vessel, Tumor stem cell, Endothelial cell,
Homing
gC1q/p32 receptor

Source & Reference

TumorHoPe2.0
TumorHoPe2
Targeted delivery system and methods of use therefor
2022
US 11512110 B2
US 11512110 B2

Other

Athymic nude mice, ApoE null mice
Phage display library
T7 phage / cyclic Cx7C and linear X7 peptide library
Nanoworms(NWs), polyethylene glycol
Evidence 5 Activity

Activity

Tumor-homing Peptides

Target

Tumor cell
Homing
Lung cancer, Breast cancer, Liver cancer, Melanoma, stomach cancer, Pancreatic cancer, Colorectal cancer, Ovarian cancer, Renal cell carcinoma, Prostate cancer, Brain tumor

Source & Reference

TumorHoPe2.0
TumorHoPe2
Noxa-derived, cell death-inducing peptide eMTD
2022
US 11248024 B2
US 11248024 B2

Other

Sp2/0, CHO (Chinese hamster ovary) K1, CHO DG44, PER.C6, W138, BHK, COS7, 293, HepG2, Huh7, 3T3, RIN, MDCK cell line
6-week-old BalB/C mice
Synthetic
necrosis-inducing peptide, Fluorescein (FAM)
Evidence 6 Activity

Activity

Tumor-homing Peptides

Target

Tumor cell, Tumor vessel, Tumor stem cell, Endothelial cell, Epithelial cell, Dendritic cell
Homing

Source & Reference

TumorHoPe2.0
TumorHoPe2
Methods and compositions using peptides and proteins with C-terminal elements
2019
US 10370245 B2
US 10370245 B2

Other

PPC-1, PC-3, Du-145, 4T1, MIA PaCa-2, PDAC1.3, B16F10, M21, and MDA-MB-435 cell line
BALB/c athymic nude mice
Phage Display library
T7 phage display library
nanoparticles, nanoworms, 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[maleimide(polyethylene glycol)2000 DSPE-PEG2000-maleimide]
Evidence 7 Activity

Activity

Tumor-homing Peptides

Target

Tumor cell
Homing
Breast cancer, Prostate cancer
fibrin and fibrin-associated clotted plasma proteins (e.g. fibronectin)

Source & Reference

TumorHoPe2.0
TumorHoPe2
Encapsulation and Storage of Therapeutic Fibrin-Homing Peptides using Conducting Polymer Nanoparticles for Programmed Release by Electrical Stimulation
2020
Anna Puiggalı-Jou et al. ACS Biomater Sci Eng. 2020 Apr 13 6(4):2135-2145.

Other

Rattus norvegicus kidney normal cells (NRK), Madin-Darby canine kidney (MDCK) cells, normal prostate epithelium(PNT2), PC3 cells
Phage display library
poly(3,4-ethylenethiophene) (PEDOT)
Evidence 8 Activity

Activity

Tumor-homing Peptides

Target

Tumor vessel
Homing
Non-small cell Lung cancer (NSCLC)
Fibrinogen

Source & Reference

TumorHoPe2.0
TumorHoPe2
Synthesis and characterization of CREKA-conjugated iron oxide nanoparticles for hyperthermia applications
2014
Anastasia M Kruse et al. Acta Biomater. 2014 Jun 10(6):2622-9.

Other

A549 lung carcinoma cells
Synthetic
Iron oxide nanoparticles (IONPs), dextran, cisplatin (CDDP)
Evidence 9 Activity

Activity

Tumor-homing Peptides

Target

Tumor cell
Homing
Non-small cell Lung cancer (NSCLC)
PD-L1

Source & Reference

TumorHoPe2.0
TumorHoPe2
Beyond Blocking: Engineering RNAi-Mediated Targeted Immune Checkpoint Nanoblocker Enables T-Cell-Independent Cancer Treatment
2020
Xiaoyan Han et al. ACS Nano. 2020 Dec 22 14(12):17524-17534.

Other

H460 non-small cell lung cancer cells, A549 cells
Healthy female C57BL/6 mice, female BALB/C nude mice
Synthetic
RNA interference (RNAi), Poly L-DOPA/silica nanoparticles (NC-NL2)
Evidence 10 Activity

Activity

Tumor-homing Peptides

Target

Tumor cell
Homing
Breast cancer

Source & Reference

TumorHoPe2.0
TumorHoPe2
Avidin-biotin interaction mediated peptide assemblies as efficient gene delivery vectors for cancer therapy
2013
Wei qu et al. Mol Pharm. 2013 Jan 7. 10(1) 261-9

Other

Human embryonic kidney transformed 293 cells (293T), human cervix carcinoma cells (HeLa), human breast cancer cells (MCF-7)
Normal male Chinese Kun Ming (KM) mice, 4−6 weeks old and weighing 18−22 g
Phage display library
TAC bioconjugates
Evidence 11 Activity

Activity

Tumor-homing Peptides

Target

Tumor cell, Interstitial cell
Homing
Breast cancer
Fibrin-fibronectin complexes

Source & Reference

TumorHoPe2.0
TumorHoPe2
Optimization and Design of Magnetic Ferrite Nanoparticles with Uniform Tumor Distribution for Highly Sensitive MRI/MPI Performance and Improved Magnetic Hyperthermia Therapy
2019
Yang Du et al. Nano Lett. 2019 Jun 12. 19(6):3618-3626.

Other

4T1 breast cancer cells
4T1 orthotopic tumor-bearing mouse
Magnetic Ferrite Nanoparticles (MFNPs), Polyethylene Glycol (PEG)
Evidence 12 Activity

Activity

Tumor-homing Peptides

Target

Tumor cell
Homing
Prostate cancer
fibrin and fibrin-associated clotted plasma proteins (e.g. fibronectin)

Source & Reference

TumorHoPe2.0
TumorHoPe2
Engineering strategy to improve peptide analogs: from structure-based computational design to tumor homing
2012
David Zanuy et al. J Comput Aided Mol Des. 2013 Jan 27(1):31-43.

Other

nude mice bearing orthotropic 22Rv1 xenograft tumors
Phage Display Library
amino dextran-coated superparamagnetic iron oxide (SPIO)
Evidence 13 Activity

Activity

Tumor-homing Peptides

Target

Tumor cell
Homing
Breast cancer

Source & Reference

TumorHoPe2.0
TumorHoPe2
Peptide targeted tripod macrocyclic Gd(III) chelates for cancer molecular MRI
2013
Zhuxian Zhou et al. Biomaterials. 2013 Oct 34(31):7683-93.

Other

4T1-GFP-Luc2 breast cancer cell line
Six-week-old female BALB/c mice
Synthetic
Tris-Gd(DOTA), Cy5.0
Evidence 14 Activity

Activity

Tumor-homing Peptides

Target

Blood vessel
Homing
Multiple tumor
Type IV collagen in basement vascular membrane

Source & Reference

TumorHoPe2.0
TumorHoPe2
Targeting cancer cells via tumor-homing peptide CREKA functional PEG nanoparticles
2016
Okur A C et al. Colloids Surf B Biointerfaces. 2016 Nov 1:147:191-200.

Other

HeLa, Human Dermal BJ Cells
Synthetic
Doxorubicin, Poly(ethylene glycol) diacrylate (PEGDA)
Evidence 15 Activity

Activity

Tumor-homing Peptides

Target

Tumor cell, tumor endothelial cell, tumor stroma
Homing
Multiple tumor
Microthrombus-associated fibrin-fibronectin complexes

Source & Reference

TumorHoPe2.0
TumorHoPe2
Specific tissue factor delivery using a tumor-homing peptide for inducing tumor infarction
2018
Shi Q et al. Biochem Pharmacol. 2018 Oct:156:501-510.

Other

4T1, MHCC97H, LS174T
wild-type or nude BALB/c mice (6-8 weeks old, 18-20 g)
Synthetic
Extracellular domain of truncated human tissue factor, FITC
Evidence 16 Activity

Activity

Tumor-homing Peptides

Target

Tumor cell
Homing
Human Breast cancers

Source & Reference

TumorHoPe2.0
TumorHoPe2
The antitumor activity of tumor-homing peptide-modified thermosensitive liposomes containing doxorubicin on MCF-7/ADR: in vitro and in vivo
2015
Wang C et al. Int J Nanomedicine. 2015 Mar 19:10:2229-48.

Other

MCF-7/ADR cell line, Human Breast Adenocarcinoma(MCF-7)
Tumor bearing nude mice
Synthetic
DSPE-PEG-maleimide, DOX-LTSL
Evidence 17 Activity

Activity

Tumor-homing Peptides

Target

Tumor vessel
Targeting
Breast cancer
Fibrin-fibronectin complexes

Source & Reference

TumorHoPe2.0
TumorHoPe2
Suppression of Tumor Energy Supply by Liposomal Nanoparticle-Mediated Inhibition of Aerobic Glycolysis
2018
Zhang Y et al. ACS Appl Mater Interfaces. 2018 Jan 24 10(3):2347-2353

Other

Human breast cancer cell line MDA-MB-231, Mouse testis stromal cells (MTS)
Tumor bearing C57BL/6 nude mice
Synthetic
DSPE-PEG-MAL
Evidence 18 Activity

Activity

Tumor-homing Peptides

Target

Tumor vessel, stroma of tumors
Homing
Prostrate cancer, Glioma, Lung cancer
Clotted-plasma proteins

Source & Reference

TumorHoPe2.0
TumorHoPe2
Weighing biointeractions between fibrin(ogen) and clot-binding peptides using microcantilever sensors
2017
Jou AP et al.J Pept Sci. 2017 Feb 23(2):162-171

Other

Phage Display Library
Poly(3,4-ethylenedioxythiophene) (PEDOT)
Evidence 19 Activity

Activity

Tumor-homing Peptides

Target

Tumor vasculature
Homing
Breast cancer, Prostate cancer
Fibrin

Source & Reference

TumorHoPe2.0
TumorHoPe2
Fibrin-binding, peptide amphiphile micelles for targeting glioblastoma
2014
Chung E J et al. Biomaterials.2014 Jan 35(4):1249-56

Other

8 - 10 weeks old male C57BL/6 mice
Synthetic
DSPE-PEG(2000)-maleimide
Evidence 20 Activity

Activity

Tumor-homing Peptides

Target

Homing
Brain tumor
Fibrin

Source & Reference

TumorHoPe2.0
TumorHoPe2
Polyethylene glycol-polylactic acid nanoparticles modified with cysteine-arginine-glutamic acid-lysine-alanine fibrin-homing peptide for glioblastoma therapy by enhanced retention effect
2014
Wu J et al. Int J Nanomedicine.2014 Nov 13:9:5261-71.

Other

U87-RFP cell line
4 - 5 weeks old male BALB/c nude mice
Synthetic
Additional Detail Fields 4 fields
Superparamagnetic nanospheres, superparamagnetic IO nanoworms (NWs) diacylglycerols, dialkylglycerols Nanoworms(NWs), polyethylene glycol necrosis-inducing peptide, Fluorescein (FAM) nanoparticles, nanoworms, 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[maleimide(polyethylene glycol)2000 DSPE-PEG2000-maleimide] poly(3,4-ethylenethiophene) (PEDOT) Iron oxide nanoparticles (IONPs), dextran, cisplatin (CDDP) RNA interference (RNAi), Poly L-DOPA/silica nanoparticles (NC-NL2) TAC bioconjugates Magnetic Ferrite Nanoparticles (MFNPs), Polyethylene Glycol (PEG) amino dextran-coated superparamagnetic iron oxide (SPIO) Tris-Gd(DOTA), Cy5.0 Doxorubicin, Poly(ethylene glycol) diacrylate (PEGDA) Extracellular domain of truncated human tissue factor, FITC DSPE-PEG-maleimide, DOX-LTSL DSPE-PEG-MAL Poly(3,4-ethylenedioxythiophene) (PEDOT) DSPE-PEG(2000)-maleimide
4T1 breast cancer cell line, MDA-MB-468 breast cancer cell line, 4306-ovarian cancer cell line, Lewis lung carcinoma (LLC) cells, hepatocellular carcinoma cells (CP20) Sp2/0, CHO (Chinese hamster ovary) K1, CHO DG44, PER.C6, W138, BHK, COS7, 293, HepG2, Huh7, 3T3, RIN, MDCK cell line PPC-1, PC-3, Du-145, 4T1, MIA PaCa-2, PDAC1.3, B16F10, M21, and MDA-MB-435 cell line Rattus norvegicus kidney normal cells (NRK), Madin-Darby canine kidney (MDCK) cells, normal prostate epithelium(PNT2), PC3 cells A549 lung carcinoma cells H460 non-small cell lung cancer cells, A549 cells Human embryonic kidney transformed 293 cells (293T), human cervix carcinoma cells (HeLa), human breast cancer cells (MCF-7) 4T1 breast cancer cells 4T1-GFP-Luc2 breast cancer cell line HeLa, Human Dermal BJ Cells 4T1, MHCC97H, LS174T MCF-7/ADR cell line, Human Breast Adenocarcinoma(MCF-7) Human breast cancer cell line MDA-MB-231, Mouse testis stromal cells (MTS) U87-RFP cell line
Mice bearing human (MDA-MB-435 human carcinoma and HT1080 human fibrosarcoma) xenograft BALB/c mice Athymic nude mice, ApoE null mice 6-week-old BalB/C mice BALB/c athymic nude mice Healthy female C57BL/6 mice, female BALB/C nude mice Normal male Chinese Kun Ming (KM) mice, 4−6 weeks old and weighing 18−22 g 4T1 orthotopic tumor-bearing mouse nude mice bearing orthotropic 22Rv1 xenograft tumors Six-week-old female BALB/c mice wild-type or nude BALB/c mice (6-8 weeks old, 18-20 g) Tumor bearing nude mice Tumor bearing C57BL/6 nude mice 8 - 10 weeks old male C57BL/6 mice 4 - 5 weeks old male BALB/c nude mice
T7 phage / cyclic Cx7C and linear X7 peptide library T7 phage display library