TPDB

3D PDB MODEL

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TPDB ID TPDB18406

Activity

Antibacterial Anticancer Antifungal Hemolysis Toxicity

Activity_category_statistics

Anti-infective peptides Cancer-related peptides Toxicity and safety peptides

Database_Source

Peptipedia CAFPdb dbAMP PD_Hemolysis

Sequence_Type

standard

Has_Modification No

Description A 6-aa standard natural multi-activity (Antibacterial, Anticancer, Antifungal, and other sources) peptide sequence curated from Peptipedia, CAFPdb, dbAMP, and other sources, with an available 3D structural model.

Sequence

CKKKRC

FASTA

>TPDB18406|Antibacterial;Anticancer;Antifungal;Hemolysis;Toxicity|length=6
CKKKRC

Formula C30H60N12O7S2

Absent Amino Acids ANDEQGHILMFPSTWYV

Common Amino Acids K

Mass 765.00

PI 10.32

Basic Residues 4

Acidic Residues 0

Hydrophobic Residues 0

Net Charge +4

Boman Index 3.30

Hydrophobicity -1.867

Aliphatic Index 0.00

Half Life

Mammalian: 1.2 hour Yeast: >20 hour E.coli: >10 hour

Extinction Coefficient Cystines 125

Absorbance 280nm 16.34

Polar Residues 2

number

0

A

1

R

0

N

0

D

2

C

0

E

0

Q

0

G

0

H

0

I

0

L

3

K

0

M

0

F

0

P

0

S

0

T

0

W

0

Y

0

V

Amino Acid Distribution

A: 0 R: 1 N: 0 D: 0 C: 2 E: 0 Q: 0 G: 0 H: 0 I: 0 L: 0 K: 3 M: 0 F: 0 P: 0 S: 0 T: 0 W: 0 Y: 0 V: 0

Length

6

Formula

C30H60N12O7S2

Mass

765.00

PI

10.32

Net Charge

+4

Boman Index

3.30

Hydrophobicity

-1.867

Evidence 1 Activity

Activity

Activity Endpoint HC50

Detail Source Activity Hemolysis

Activity Measurement 71

Hemolysis Raw

Human erythrocytes:HC50=71±6.4µg/ml

Target

RBC Target Human erythrocytes

Source & Reference

Database Source Hemolytik PD_Hemolysis

Source DRAMP4

Reference

Int J Mol Sci. 2020 Sep 29 21(19):7208.

Other

Source Activity Label	Source Definition
Hemolysis	Hemolytik experimentally validated hemolytic peptide annotation.

Evidence 2 Activity

Activity

Activity Endpoint 50% Hemolysis

Detail Source Activity Toxicity

Activity Measurement 71±6.4 µg/mL

Toxicity Type Hemolytic Cytotoxic

Toxicity Raw

50% Hemolysis | 71±6.4 | µg/mL | target cell: Human erythrocytes

Target

Target Cell Human erythrocytes

Source & Reference

Database Source NA

Source Database DBAASP

Reference

Effect of Disulfide Cyclization of Ultrashort Cationic Lipopeptides on Antimicrobial Activity and Cytotoxicity. | Int J Mol Sci | 2020

Evidence 3 Activity

Activity

Activity Endpoint IC50

Detail Source Activity Toxicity

Activity Measurement 45.7±13.5 µg/mL

Toxicity Type Cytotoxic

Toxicity Raw

IC50 | 45.7±13.5 | µg/mL | target cell: Human keratinocytes HaCat

Target

Target Cell Human keratinocytes HaCat

Source & Reference

Database Source NA

Source Database DBAASP

Reference

Effect of Disulfide Cyclization of Ultrashort Cationic Lipopeptides on Antimicrobial Activity and Cytotoxicity. | Int J Mol Sci | 2020

Evidence 4 Activity

Activity

Activity Endpoint HC50

Detail Source Activity Toxicity

Activity Measurement 71

Toxicity Type Hemolytic Cytotoxic

Toxicity Raw

Hemolytic_activity: Human erythrocytes:HC50=71±6.4µg/ml | Cytotoxicity: HaCat: IC50=45.7±13.5µg/ml

Target

Target Cell erythrocytes

Source & Reference

Database Source NA

Source Database DRAMP

Reference

Int J Mol Sci. 2020 Sep 29 21(19):7208.

Evidence 5 Hemolysis

Activity

Activity Measurement 71

Activity Endpoint HC50

Hemolysis Raw

Human erythrocytes:HC50=71±6.4µg/ml

Detail Source Activity Hemolysis

Target

RBC Target Human erythrocytes

Source & Reference

source PD_Hemolysis

Reference

Int J Mol Sci. 2020 Sep 29 21(19):7208.

Source DRAMP4

Database Source PD_Hemolysis