TPDB

3D PDB MODEL

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TPDB ID TPDB18217

Activity

Antibacterial Anticancer Toxicity

Activity_category_statistics

Anti-infective peptides Cancer-related peptides Toxicity and safety peptides

Database_Source

Peptipedia

Sequence_Type

standard

Has_Modification No

Description A 40-aa standard natural multi-activity (Antibacterial, Anticancer, and Toxicity) peptide sequence curated from Peptipedia, with an available 3D structural model.

Sequence

ATCDLLSPFKVGHAACAAHCIARGKRGGWCDGRAVCNCRK

FASTA

>TPDB18217|Antibacterial;Anticancer;Toxicity|length=40
ATCDLLSPFKVGHAACAAHCIARGKRGGWCDGRAVCNCRK

Formula C175H285N61O48S6

Absent Amino Acids EQMY

Common Amino Acids A

Mass 4203.93

PI 8.92

Basic Residues 9

Acidic Residues 2

Hydrophobic Residues 14

Net Charge +5

Boman Index 4.88

Hydrophobicity -0.042

Aliphatic Index 61.25

Half Life

Mammalian: 4.4 hour Yeast: >20 hour E.coli: >10 hour

Extinction Coefficient Cystines 5875

Absorbance 280nm 139.75

Polar Residues 9

number

7

A

4

R

1

N

2

D

6

C

0

E

0

Q

5

G

2

H

1

I

2

L

3

K

0

M

1

F

1

P

1

S

1

T

1

W

0

Y

2

V

Amino Acid Distribution

A: 7 R: 4 N: 1 D: 2 C: 6 E: 0 Q: 0 G: 5 H: 2 I: 1 L: 2 K: 3 M: 0 F: 1 P: 1 S: 1 T: 1 W: 1 Y: 0 V: 2

Length

40

Formula

C175H285N61O48S6

Mass

4203.93

PI

8.92

Net Charge

+5

Boman Index

4.88

Hydrophobicity

-0.042

Evidence 1 Activity

Activity

Activity Endpoint MIC

Detail Source Activity Antibacterial

Activity Measurement 0.95 µM

Target

Target Organism Staphylococcus aureus CVCC 546

Source & Reference

Database Source NA

Other

Evidence Text

Active against Gram+ S. aureus CVCC 546 (MIC 0.95 uM), S. epidermidis ATCC 12228 (MIC 1.91 uM), S. pneumoniae CVCC 2350 (MIC 0.95 uM), and S. s

Evidence 2 Activity

Activity

Activity Endpoint MIC

Detail Source Activity Antibacterial

Activity Measurement 1.91 µM

Target

Target Organism Staphylococcus epidermidis ATCC 12228

Source & Reference

Database Source NA

Other

Evidence Text

Active against Gram+ S. aureus CVCC 546 (MIC 0.95 uM), S. epidermidis ATCC 12228 (MIC 1.91 uM), S. pneumoniae CVCC 2350 (MIC 0.95 uM), and S. suis CVCC 3928 (MIC 0.95 uM), stronger tha

Evidence 3 Activity

Activity

Activity Endpoint MIC

Detail Source Activity Antibacterial

Activity Measurement 0.95 µM

Target

Target Organism S. pneumoniae CVCC 2350

Source & Reference

Database Source NA

Other

Evidence Text

against Gram+ S. aureus CVCC 546 (MIC 0.95 uM), S. epidermidis ATCC 12228 (MIC 1.91 uM), S. pneumoniae CVCC 2350 (MIC 0.95 uM), and S. suis CVCC 3928 (MIC 0.95 uM), stronger than the WT DLP4 (see AP2548). Not good at

Evidence 4 Activity

Activity

Activity Endpoint MIC

Detail Source Activity Antibacterial

Activity Measurement 0.95 µM

Target

Target Organism S. suis CVCC 3928

Source & Reference

Database Source NA

Other

Evidence Text

uM), S. epidermidis ATCC 12228 (MIC 1.91 uM), S. pneumoniae CVCC 2350 (MIC 0.95 uM), and S. suis CVCC 3928 (MIC 0.95 uM), stronger than the WT DLP4 (see AP2548). Not good at Gram- E-coli ATCC 25922 or K88 (MIC

Evidence 5 Activity

Activity

Activity Endpoint MIC

Detail Source Activity Antibacterial

Activity Measurement >30.5 µM

Target

Target Organism S. pullorum CVCC 533

Source & Reference

Database Source NA

Other

Evidence Text

than the WT DLP4 (see AP2548). Not good at Gram- E-coli ATCC 25922 or K88 (MIC >30.5 uM), S. pullorum CVCC 533 (MIC >30.5 uM), and S. enteritidis CVCC 3377 (MIC >30.5 uM, i.e., >128 ug/ml).

Evidence 6 Activity

Activity

Activity Endpoint 0.38% Hemolysis

Detail Source Activity Toxicity

Activity Measurement 128 µg/mL

Toxicity Type Hemolytic Cytotoxic

Toxicity Raw

0.38% Hemolysis | 128 | µg/mL | target cell: Mouse erythrocytes

Target

Target Cell Mouse erythrocytes

Source & Reference

Database Source NA

Source Database DBAASP

Reference

An Enhanced Variant Designed From DLP4 Cationic Peptide Against Staphylococcus aureus CVCC 546. | Front Microbiol | 2020