TPDB

3D PDB MODEL

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TPDB ID TPDB17313

Activity

Antibacterial Antifungal Toxicity

Activity_category_statistics

Anti-infective peptides Toxicity and safety peptides

Database_Source

dbAMP Peptipedia CAFPdb

Sequence_Type

standard

Has_Modification No

Description A 45-aa standard natural multi-activity (Antibacterial, Antifungal, and Toxicity) peptide sequence curated from dbAMP, Peptipedia, and CAFPdb, with an available 3D structural model.

Sequence

GWWKRVFKPVERLGQRVRDAGIQGLQIAQQGANVLATVRGGPPQQ

FASTA

>TPDB17313|Antibacterial;Antifungal;Toxicity|length=45
GWWKRVFKPVERLGQRVRDAGIQGLQIAQQGANVLATVRGGPPQQ

Formula C221H358N72O59

Absent Amino Acids CHMSY

Common Amino Acids QG

Mass 4967.72

PI 12.24

Basic Residues 7

Acidic Residues 2

Hydrophobic Residues 17

Net Charge +5

Boman Index 7.86

Hydrophobicity -0.533

Aliphatic Index 84.44

Half Life

Mammalian: 30 hour Yeast: >20 hour E.coli: >10 hour

Extinction Coefficient Cystines 11000

Absorbance 280nm 221.43

Polar Residues 9

number

4

A

5

R

1

N

1

D

0

C

1

E

7

Q

7

G

0

H

2

I

3

L

2

K

0

M

1

F

3

P

0

S

1

T

2

W

0

Y

5

V

Amino Acid Distribution

A: 4 R: 5 N: 1 D: 1 C: 0 E: 1 Q: 7 G: 7 H: 0 I: 2 L: 3 K: 2 M: 0 F: 1 P: 3 S: 0 T: 1 W: 2 Y: 0 V: 5

Length

45

Formula

C221H358N72O59

Mass

4967.72

PI

12.24

Net Charge

+5

Boman Index

7.86

Hydrophobicity

-0.533

Evidence 1 Activity

Activity

Activity Endpoint MIC

Detail Source Activity Antibacterial

Activity Measurement 1-2 µM

Target

Target Organism Escherichia coli ATCC 8739

Source & Reference

Database Source dbAMP

Other

Source Activity Label	Source Definition
Antibacterial	dbAMP classified as Antibacterial under the Anti-bacterial function class.

Evidence Text

active against S-aureus ATCC 6538 (MIC >32-64 uM), E. coli ATCC 8739 (MIC 1-2 uM), P. aeruginos (MIC 1-2 uM).

Evidence 2 Activity

Activity

Activity Endpoint MIC

Detail Source Activity Antibacterial

Activity Measurement 1-2 µM

Target

Target Organism P. aeruginos

Source & Reference

Database Source dbAMP

Other

Evidence Text

active against S-aureus ATCC 6538 (MIC >32-64 uM), E. coli ATCC 8739 (MIC 1-2 uM), P. aeruginos (MIC 1-2 uM).

Evidence 3 Activity

Activity

Activity Endpoint MIC

Detail Source Activity Antifungal

Activity Measurement 1-2 µM

Target

Target Organism Escherichia coli ATCC 8739

Source & Reference

Database Source dbAMP CAFPdb

Other

Source Activity Label	Source Definition
Antifungal	dbAMP classified under the Anti-fungal function class.

Evidence Text

active against S-aureus ATCC 6538 (MIC >32-64 uM), E. coli ATCC 8739 (MIC 1-2 uM), P. aeruginos (MIC 1-2 uM).

Evidence 4 Activity

Activity

Activity Endpoint MIC

Detail Source Activity Antifungal

Activity Measurement 1-2 µM

Target

Target Organism P. aeruginos

Source & Reference

Database Source dbAMP CAFPdb

Other

Evidence Text

active against S-aureus ATCC 6538 (MIC >32-64 uM), E. coli ATCC 8739 (MIC 1-2 uM), P. aeruginos (MIC 1-2 uM).

Evidence 5 Activity

Activity

Activity Endpoint IC50

Detail Source Activity Toxicity

Activity Measurement 37.37 µM

Toxicity Type Cytotoxic

Toxicity Raw

IC50 | 37.37 | µM | target cell: Human fibroblasts MRC-5 SV2

Target

Target Cell Human fibroblasts MRC-5 SV2

Source & Reference

Database Source NA

Source Database DBAASP

Reference

In Vitro Evaluation of Antimicrobial Peptides from the Black Soldier Fly (Hermetia Illucens) against a Selection of Human Pathogens. | Microbiol Spectr | 2022