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Peptide record

TPDB16889

Pugnin A Antibacterial Anticancer Hemolysis Toxicity standard
13 amino acids
Basic Information
3D PDB MODEL
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TPDB16889
Pugnin A
Antibacterial Anticancer Hemolysis Toxicity
Anti-infective peptides Cancer-related peptides Toxicity and safety peptides
dbAMP Peptipedia CancerPPD 2.0 PD_Hemolysis
standard
No
A 13-aa standard natural multi-activity (Antibacterial, Anticancer, Hemolysis, and other sources) peptide sequence curated from dbAMP, Peptipedia, CancerPPD 2.0, and other sources, with an available 3D structural model.
Sequence
RLMRIFRILKLAR
Physicochemical Analysis
C77H140N26O14S1
NDCEQGHPSTWYV
R
1686.18
12.88
5
0
8
+5
0.40
0.385
157.69
Mammalian: 1 hour Yeast: 2 min E.coli: 2 min
0
0.00
0
Residue Composition
number
1
A
4
R
0
N
0
D
0
C
0
E
0
Q
0
G
0
H
2
I
3
L
1
K
1
M
1
F
0
P
0
S
0
T
0
W
0
Y
0
V
Amino Acid Distribution
A: 1 R: 4 N: 0 D: 0 C: 0 E: 0 Q: 0 G: 0 H: 0 I: 2 L: 3 K: 1 M: 1 F: 1 P: 0 S: 0 T: 0 W: 0 Y: 0 V: 0
Chemical Descriptors
13
C77H140N26O14S1
1686.18
12.88
+5
0.40
0.385
Free
L
Linear
Free
Antibacterial and Anticancer
Synthetic Using Skin Transcriptome of Treefrog ( Boana pugnax)
Evidence Records 7 records
Evidence 1 Activity

Activity

MIC50
Antibacterial
0.70 µM

Target

Enterococcus faecalis ATCC 29212

Source & Reference

dbAMP

Other

Source Activity Label Source Definition
Antibacterial
dbAMP classified as Antibacterial under the Anti-bacterial function class.
active against S. aureus ATCC 25923 (MIC50 11.0 uM, MIC90 148.9 uM), E. faecalis ATCC 29212 (MIC50 0.70 uM), MIC90 107.2 uM), P. aeruginosa ATCC 27853 (MIC50 4.1 uM MIC90 28.5 uM), E. coli ATCC 25
Evidence 2 Activity

Activity

12% Cytotoxicity at 100 µM
Anticancer

Target

Breast Cancer
MCF-7
Breast

Assay & Model

MTT assay
24-h

Source & Reference

CancerPPD 2.0
CancerPPD2
2021

Other

Source Activity Label Source Definition
Anticancer
CancerPPD 2.0 manually curated experimentally verified anticancer peptide/protein annotation.
Evidence 3 Activity

Activity

74% Cytotoxicity at 100 µM
Anticancer

Target

Prostate Cancer
PC-3
Prostate

Assay & Model

MTT assay
24-h

Source & Reference

CancerPPD 2.0
CancerPPD2
2021
Evidence 4 Activity

Activity

50% Hemolysis
Toxicity
17 µM
Hemolytic Cytotoxic
50% Hemolysis | 17 | µM | target cell: Human erythrocytes

Target

Human erythrocytes

Source & Reference

NA
DBAASP
In Silico Selection and Evaluation of Pugnins with Antibacterial and Anticancer Activity Using Skin Transcriptome of Treefrog ( Boana pugnax). | Pharmaceutics | 2021
Evidence 5 Activity

Activity

60% Cytotoxicity
Toxicity
100 µM
Cytotoxic
60% Cytotoxicity | 100 | µM | target cell: Human keratinocytes HaCat

Target

Human keratinocytes HaCat

Source & Reference

NA
DBAASP
In Silico Selection and Evaluation of Pugnins with Antibacterial and Anticancer Activity Using Skin Transcriptome of Treefrog ( Boana pugnax). | Pharmaceutics | 2021
Evidence 6 Activity

Activity

Toxicity
Hemolytic Cytotoxic
Hemolytic_activity: Not available | Cytotoxicity: Not available

Target

erythrocytes

Source & Reference

NA
DRAMP
Pharmaceutics. 2021 Apr 18 13(4):578.
Evidence 7 Hemolysis

Source & Reference

PD_Hemolysis
PD_Hemolysis