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Peptide record

TPDB14337

HIV-TAT48-57 P-T12-Pir TAT Tat (tFGF2) Tat (TG) Tat-MT TAT0 TAT12 TAT4 Antibacterial Anticancer Antiviral Cell-penetrating Peptides standard
10 amino acids
Basic Information
3D PDB MODEL
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TPDB14337
HIV-TAT48-57 P-T12-Pir TAT Tat (tFGF2) Tat (TG) Tat-MT TAT0 TAT12 TAT4
Antibacterial Anticancer Antiviral Cell-penetrating Peptides
Anti-infective peptides Cancer-related peptides Delivery and barrier-penetrating peptides
dbAMP Peptipedia AntiCP 2.0 CancerPPD 2.0 SATPdb dbACP iACP-DRLF AI4AVP dbAMP 3.0 DRAVP 2.0 CellPPD CPPsite3.0
standard
No
A 10-aa standard natural multi-activity (Antibacterial, Anticancer, Antiviral, and other sources) peptide sequence curated from dbAMP, Peptipedia, AntiCP 2.0, and other sources, with an available 3D structural model.
Sequence
GRKKRRQRRR
Physicochemical Analysis
C55H109N31O12
ANDCEHILMFPSTWYV
R
1396.67
13.10
8
0
0
+8
7.43
-3.870
0.00
Mammalian: 30 hour Yeast: >20 hour E.coli: >10 hour
0
0.00
1
Residue Composition
number
0
A
6
R
0
N
0
D
0
C
0
E
1
Q
1
G
0
H
0
I
0
L
2
K
0
M
0
F
0
P
0
S
0
T
0
W
0
Y
0
V
Amino Acid Distribution
A: 0 R: 6 N: 0 D: 0 C: 0 E: 0 Q: 1 G: 1 H: 0 I: 0 L: 0 K: 2 M: 0 F: 0 P: 0 S: 0 T: 0 W: 0 Y: 0 V: 0
Chemical Descriptors
10
C55H109N31O12
1396.67
13.10
+8
7.43
-3.870
Free
L
Cationic
Linear
Linear
Free
Anticancer
Not Available
NCC(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCC(=O)N)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)O N[C@@H](C)C(=O)N[C@@H](CS)C(=O)N[C@@H]([C@@H](C)O)C(=O)NCC(=O)N[C@@H](CO)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CCC(=O)N)C(=O)N[C@@H](Cc1[nH]cnc1)C(=O)N[C@@H](CCC(=O)N)C(=O)N[C@@H](CS)C(=O)NCC(=O)O N[C@@H](CO)C(=O)NCC(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CCC(=O)N)C(=O)N[C@@H](CCC(=O)O)C(=O)N[C@@H](CCC(=O)O)C(=O)N[C@@H](Cc1ccccc1)C(=O)O N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)O N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)O N[C@@H](C(C)C)C(=O)N1CCC[C@H]1C(=O)N[C@@H](CCSC)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCCN)C(=O)O N1CCC[C@H]1C(=O)N[C@@H](Cc1ccccc1)C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](Cc1ccccc1)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H]([C@@H](C)CC)C(=O)O
Evidence Records 14 records
Evidence 1 Activity

Activity

7% apoptosis at 10 µM
Anticancer

Target

Cervical Cancer
HeLa
Cervix

Assay & Model

LDH leakage assay
16 to 24-h

Source & Reference

dbAMP 3.0 CancerPPD 2.0 dbACP SATPdb
CancerPPD2
2004

Other

Source Activity Label Source Definition
Anticancer
CancerPPD 2.0 manually curated experimentally verified anticancer peptide/protein annotation. dbAMP 3.0 classified as Anticancer under the disease-associated peptide function classes.
Evidence 2 Activity

Activity

15% apoptosis at 20 µM
Anticancer

Target

Cervical Cancer
HeLa
Cervix

Assay & Model

LDH leakage assay
16 to 24-h

Source & Reference

dbAMP 3.0 CancerPPD 2.0 dbACP SATPdb
CancerPPD2
2004
Evidence 3 Activity

Activity

35% apoptosis at 30 µM
Anticancer

Target

Cervical Cancer
HeLa
Cervix

Assay & Model

LDH leakage assay
16 to 24-h

Source & Reference

dbAMP 3.0 CancerPPD 2.0 dbACP SATPdb
CancerPPD2
2004
Evidence 4 Activity

Activity

65% apoptosis at 50 µM
Anticancer

Target

Cervical Cancer
HeLa
Cervix

Assay & Model

LDH leakage assay
16 to 24-h

Source & Reference

dbAMP 3.0 CancerPPD 2.0 dbACP SATPdb
CancerPPD2
2004
Evidence 5 Activity

Activity

Cell-penetrating Peptides

Target

H9c2 cells

Assay & Model

Streptozotocin-induced diabetic mouse model
Streptozotocin-induced diabetic mouse model

Source & Reference

CPPsite3.0
CPPsite3

Other

Source Activity Label Source Definition
Cell-penetrating Peptides
CPPsite3.0 experimentally validated cell-penetrating peptide annotation.
Natural residues Linear L Cationic
Protein derived
Protein (GFP, anti-oxidative metallothionein protein)
In vitro and in vivo
CCCCCCTTCC
Evidence 6 Activity

Activity

Cell-penetrating Peptides

Target

Beas-2B Cells

Assay & Model

Female 6-week-old BALB/c mice
Female 6-week-old BALB/c mice

Source & Reference

CPPsite3.0
CPPsite3

Other

Natural residues Linear L Cationic
Protein derived
PLD1-specific binding motif (TVTSP)-FAM labelled
Green fluorescence was detected in almost all cells 1 h after exposure to TAT-TVTSP-FAM, and the signals were strongest after 2 h
In vitro and in vivo
CCCCCCCCCC
Evidence 7 Activity

Activity

Cell-penetrating Peptides

Target

Bbecs Or Rat Astrocytes And Intestine (Caco-2 Assay) And A Bbb Model

Source & Reference

CPPsite3.0
CPPsite3

Other

Natural residues Linear L
Protein derived
carboxyfuorescein (Cf) at N -terminal
Three CPPs (TAT, penetratin and R8) showed the highest internalization profiles in both cell types. TAT was not detected by MALDI-TOF MS in acceptor wells
In vitro
CCCCTTC
Evidence 8 Activity

Activity

Cell-penetrating Peptides

Target

Pancreatic Islets Were Isolated From Outbred Sd Rats

Assay & Model

Male Balb/c mice aged 7 weeks
Male Balb/c mice aged 7 weeks

Source & Reference

CPPsite3.0
CPPsite3

Other

Natural residues Linear L Cationic
Protein derived
Metallothionein (MT)
No green fluorescence with fluorescence-labeled MT,however, for the treatment of fluorescence-labelled Tat-MT fusion protein, all most islets showed green fluorescence,
In vitro and in vivo
CCSGGGGGGGGCCCCC
Evidence 9 Activity

Activity

Cell-penetrating Peptides

Target

Hdf

Source & Reference

CPPsite3.0
CPPsite3

Other

Natural residues Linear L Cationic
Protein derived
FITC and hFGF2
Ara27-FITC translocated into the cells more than Tat-FITC
In vitro
CCCCCCCC
Evidence 10 Activity

Activity

Cell-penetrating Peptides

Target

HeLa Cells

Source & Reference

CPPsite3.0
CPPsite3

Other

Non-natural residues Linear L Cationic
Synthetic
pHPMA polymer
Cytoplasm
Internalized about 40 times more efficiently than the control polymer
Endocytosis
In vitro
Evidence 11 Activity

Activity

Cell-penetrating Peptides

Target

HeLa Cells

Source & Reference

CPPsite3.0
CPPsite3

Other

Natural residues Linear L Cationic
Synthetic
pHPMA polymer
Cytoplasm
7 times higher cell penetration level compared with REF at 1 μM
Endocytosis
In vitro
CCSCC
Evidence 12 Activity

Activity

Cell-penetrating Peptides

Target

HeLa Cells

Source & Reference

CPPsite3.0
CPPsite3

Other

Natural residues Linear L Cationic
Synthetic
pHPMA polymer
Cytoplasm
Exhibits 2.8 times higher penetration efficacy than conjugate TAT0
Endocytosis
In vitro
CCCCCC
Evidence 13 Activity

Activity

Cell-penetrating Peptides

Target

HeLa Cells

Source & Reference

CPPsite3.0
CPPsite3

Other

Natural residues Linear L Cationic
Synthetic
Pirarubicin (Pir)
Cytoplasm
Endocytosis
In vitro
CCCCCCTTCC
Evidence 14 Activity

Activity

Cell-penetrating Peptides

Target

HeLa Cells

Source & Reference

CPPsite3.0
CPPsite3

Other

Non-natural residues Linear L Cationic
Protein derived
mCherry protein
Cytoplasm
Lower than circular counterpart
In vitro
Additional Detail Fields 1 fields
CCCCCCTTCC CCCCCCCCCC CCCCTTC CCSGGGGGGGGCCCCC CCCCCCCC CCSCC CCCCCC