TPDB

3D PDB MODEL

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TPDB ID TPDB14003

Peptide_Name

Raniseptin-3

Activity

Antibacterial Anticancer

Activity_category_statistics

Anti-infective peptides Cancer-related peptides

Database_Source

dbAMP Peptipedia CancerPPD 2.0 dbACP

Sequence_Type

standard

Has_Modification No

Description A 28-aa standard natural multi-activity (Antibacterial and Anticancer) peptide sequence curated from dbAMP, Peptipedia, CancerPPD 2.0, and other sources, with an available 3D structural model.

Sequence

AWLDKLKSIGKVVGKVAIGVAKNLLNPQ

FASTA

>TPDB14003|Antibacterial;Anticancer|length=28
AWLDKLKSIGKVVGKVAIGVAKNLLNPQ

Formula C137H235N37O35

Absent Amino Acids RCEHMFTY

Common Amino Acids K

Mass 2960.60

PI 10.71

Basic Residues 5

Acidic Residues 1

Hydrophobic Residues 14

Net Charge +4

Boman Index 3.27

Hydrophobicity 0.300

Aliphatic Index 135.71

Half Life

Mammalian: 4.4 hour Yeast: >20 hour E.coli: >10 hour

Extinction Coefficient Cystines 5500

Absorbance 280nm 185.77

Polar Residues 4

number

3

A

0

R

2

N

1

D

0

C

0

E

1

Q

3

G

0

H

2

I

4

L

5

K

0

M

0

F

1

P

1

S

0

T

1

W

0

Y

4

V

Amino Acid Distribution

A: 3 R: 0 N: 2 D: 1 C: 0 E: 0 Q: 1 G: 3 H: 0 I: 2 L: 4 K: 5 M: 0 F: 0 P: 1 S: 1 T: 0 W: 1 Y: 0 V: 4

Length

28

Formula

C137H235N37O35

Mass

2960.60

PI

10.71

Net Charge

+4

Boman Index

3.27

Hydrophobicity

0.300

C_terminal_Modification

Free

Chirality

L

Linear_or_Cyclic

Linear

N_terminal_Modification

Free

Nature

Antimicrobial and Antitumor

Origin

Boana raniceps

Evidence 1 Activity

Activity

Activity Endpoint MIC

Detail Source Activity Antibacterial

Activity Measurement 2 µM

Target

Target Organism Escherichia coli ATCC 25922

Source & Reference

Database Source dbAMP

Other

Source Activity Label	Source Definition
Antibacterial	dbAMP classified as Antibacterial under the Anti-bacterial function class.

Evidence Text

Gram- E. coli ATCC 25922 (MIC 2 uM), K. pneumoniae ATCC 13883 (MIC 1 uM), K. pneumoniae ATCC 13883 (MIC 4 uM), Gram+ S. aureu

Evidence 2 Activity

Activity

Activity Endpoint MIC

Detail Source Activity Antibacterial

Activity Measurement 1 µM

Target

Target Organism Klebsiella pneumoniae ATCC 13883

Source & Reference

Database Source dbAMP

Other

Evidence Text

Gram- E. coli ATCC 25922 (MIC 2 uM), K. pneumoniae ATCC 13883 (MIC 1 uM), K. pneumoniae ATCC 13883 (MIC 4 uM), Gram+ S. aureus ATCC 25923 (MIC 4 uM), S. epidermid

Evidence 3 Activity

Activity

Activity Endpoint MIC

Detail Source Activity Antibacterial

Activity Measurement 4 µM

Target

Target Organism Klebsiella pneumoniae ATCC 13883

Source & Reference

Database Source dbAMP

Other

Evidence Text

Gram- E. coli ATCC 25922 (MIC 2 uM), K. pneumoniae ATCC 13883 (MIC 1 uM), K. pneumoniae ATCC 13883 (MIC 4 uM), Gram+ S. aureus ATCC 25923 (MIC 4 uM), S. epidermidis ATCC 12228 (MIC 8 uM), and yeast C

Evidence 4 Activity

Activity

Activity Endpoint MIC

Detail Source Activity Antibacterial

Activity Measurement 4 µM

Target

Target Organism Staphylococcus aureus ATCC 25923

Source & Reference

Database Source dbAMP

Other

Evidence Text

IC 2 uM), K. pneumoniae ATCC 13883 (MIC 1 uM), K. pneumoniae ATCC 13883 (MIC 4 uM), Gram+ S. aureus ATCC 25923 (MIC 4 uM), S. epidermidis ATCC 12228 (MIC 8 uM), and yeast C.albicans ATCC 14053 (MIC > 128 uM).

Evidence 5 Activity

Activity

Activity Endpoint MIC

Detail Source Activity Antibacterial

Activity Measurement 8 µM

Target

Target Organism Staphylococcus epidermidis ATCC 12228

Source & Reference

Database Source dbAMP

Other

Evidence Text

3 (MIC 1 uM), K. pneumoniae ATCC 13883 (MIC 4 uM), Gram+ S. aureus ATCC 25923 (MIC 4 uM), S. epidermidis ATCC 12228 (MIC 8 uM), and yeast C.albicans ATCC 14053 (MIC > 128 uM).

Evidence 6 Activity

Activity

Activity Endpoint IC50

Activity Raw

IC50 = 6.56 uM

Detail Source Activity Anticancer

Activity Measurement 6.56 µM

Target

Cancer Type Skin Cancer

Cell Line B16-F10

Tissue Affected Skin

Assay & Model

Assay MTT assay

Testing Time 3-h

Source & Reference

Database Source CancerPPD 2.0 dbACP

Source CancerPPD2

Year 2023

Other

Source Activity Label	Source Definition
Anticancer	CancerPPD 2.0 manually curated experimentally verified anticancer peptide/protein annotation.