TPDB

3D PDB MODEL

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TPDB ID TPDB13791

Activity

Antibacterial Toxicity

Activity_category_statistics

Anti-infective peptides Toxicity and safety peptides

Database_Source

AntiBP3 Peptipedia

Sequence_Type

standard

Has_Modification No

Description A 40-aa standard natural multi-activity (Antibacterial and Toxicity) peptide sequence curated from AntiBP3 and Peptipedia, with an available 3D structural model.

Sequence

GFGCNGPWDEDDMKCHNHCKSIKGYKGGYCAKAGFVCKCY

FASTA

>TPDB13791|Antibacterial;Toxicity|length=40
GFGCNGPWDEDDMKCHNHCKSIKGYKGGYCAKAGFVCKCY

Formula C191H279N53O55S7

Absent Amino Acids RQLT

Common Amino Acids G

Mass 4422.06

PI 8.01

Basic Residues 8

Acidic Residues 4

Hydrophobic Residues 11

Net Charge +2

Boman Index 5.88

Hydrophobicity -0.650

Aliphatic Index 22.00

Half Life

Mammalian: 30 hour Yeast: >20 hour E.coli: >10 hour

Extinction Coefficient Cystines 10345

Absorbance 280nm 233.94

Polar Residues 12

number

2

A

0

R

2

N

3

D

6

C

1

E

0

Q

7

G

2

H

1

I

0

L

6

K

1

M

2

F

1

P

1

S

0

T

1

W

3

Y

1

V

Amino Acid Distribution

A: 2 R: 0 N: 2 D: 3 C: 6 E: 1 Q: 0 G: 7 H: 2 I: 1 L: 0 K: 6 M: 1 F: 2 P: 1 S: 1 T: 0 W: 1 Y: 3 V: 1

Length

40

Formula

C191H279N53O55S7

Mass

4422.06

PI

8.01

Net Charge

+2

Boman Index

5.88

Hydrophobicity

-0.650

Evidence 1 Activity

Activity

Detail Source Activity Antibacterial

Source & Reference

Database Source NA

Other

Evidence Text

inhibition-zone assay: active against Gram+ B. megaterium CGMCC 1.0459 (lethal conc CL 0.67 uM), C. luteum (LC 0.03 uM), S. aureus CGMCC 1.89 (LC 1.22 uM), Penicillin-sensitive S. epidermidis P1111 (LC 4.03 uM), Penicillin-resistant S. epidermidis P1111 (LC 4.38 uM), Methicillin-resistant S. aureus (MRSA) P1374 (LC 2.69 uM), Methicillin-resistant coagulase negative Staphylococci P1369 (CL 4.78 uM), MRSA P1386 (CL 2.51 uM), S. aureus J685, S. aureus J698 (CL 2.69 uM), S. aureus J706 (CL 2.51 uM), and S. warneri CGMCC 1.2824 (CL 1.06 uM). Usually, LC is comparable to MIC. Serum effect: activity remained in the presence of mouse serum (% unknown).

Evidence 2 Activity

Activity

Activity Endpoint 0% Hemolysis

Detail Source Activity Toxicity

Activity Measurement 25 µM

Toxicity Type Hemolytic Cytotoxic

Toxicity Raw

0% Hemolysis | 25 | µM | target cell: Mouse erythrocytes

Target

Target Cell Mouse erythrocytes

Source & Reference

Database Source NA

Source Database DBAASP

Reference

A Fungal Defensin Inhibiting Bacterial Cell-Wall Biosynthesis with Non-Hemolysis and Serum Stability | J Fungi (Basel) | 2022