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Peptide record

TPDB13643

LFB Antibacterial Anticancer Antifungal Hemolysis Toxicity standard

33 amino acids

3D PDB MODEL

Drag to rotate. Click a residue or atom to inspect it; the selected residue is highlighted in amber.

TPDB ID TPDB13643

Peptide_Name

LFB

Activity

Antibacterial Anticancer Antifungal Hemolysis Toxicity

Activity_category_statistics

Anti-infective peptides Cancer-related peptides Toxicity and safety peptides

Database_Source

AntiBP3 Peptipedia CancerPPD 2.0 dbACP AFP-GFuse Antifungipept CAFPdb dbAMP PD_Hemolysis ToxinPred 3.0

Sequence_Type

standard

Has_Modification No

Description A 33-aa standard natural multi-activity (Antibacterial, Anticancer, Antifungal, and other sources) peptide sequence curated from AntiBP3, Peptipedia, CancerPPD 2.0, and other sources, with an available 3D structural model.

Sequence

GLFSVVKGVLKGVGKNVSGSLLDQLKCKISGGC

FASTA

>TPDB13643|Antibacterial;Anticancer;Antifungal;Hemolysis;Toxicity|length=33
GLFSVVKGVLKGVGKNVSGSLLDQLKCKISGGC

Formula C145H252N40O42S2

Absent Amino Acids AREHMPTWY

Common Amino Acids G

Mass 3291.96

PI 10.06

Basic Residues 5

Acidic Residues 1

Hydrophobic Residues 12

Net Charge +4

Boman Index 3.40

Hydrophobicity 0.494

Aliphatic Index 114.85

Half Life

Mammalian: 30 hour Yeast: >20 hour E.coli: >10 hour

Extinction Coefficient Cystines 125

Absorbance 280nm 3.80

Polar Residues 8

number

Amino Acid Distribution

A: 0 R: 0 N: 1 D: 1 C: 2 E: 0 Q: 1 G: 7 H: 0 I: 1 L: 5 K: 5 M: 0 F: 1 P: 0 S: 4 T: 0 W: 0 Y: 0 V: 5

Length

Formula

C145H252N40O42S2

Mass

3291.96

10.06

Net Charge

Boman Index

3.40

Hydrophobicity

0.494

C_terminal_Modification

Free

Chirality

Linear_or_Cyclic

Linear

N_terminal_Modification

Free

Nature

Antimicrobial

Origin

Limnonectes fujianensi Limnonectes fujianensis

Evidence 1 Activity

Activity

Detail Source Activity Antibacterial

Source & Reference

Database Source dbAMP

Other

Source Activity Label	Source Definition
Antibacterial	dbAMP classified as Antibacterial under the Anti-bacterial function class.

Evidence Text

Active against S. aureus (MIC 16 mg/L), E. coli (MIC 32 mg/L), and yeast C. albicans (MIC 64 mg/L).

Evidence 2 Activity

Activity

Activity Endpoint IC50

Activity Raw

IC50 = 3.47 µM

Detail Source Activity Anticancer

Activity Measurement 3.47 µM

Target

Cancer Type Lung Cancer

Cell Line H-460

Tissue Affected Lung

Assay & Model

Assay MTT assay

Testing Time 24-h

Source & Reference

Database Source CancerPPD 2.0 dbACP

Source CancerPPD2

Year 2019

Other

Source Activity Label	Source Definition
Anticancer	CancerPPD 2.0 manually curated experimentally verified anticancer peptide/protein annotation.

Evidence 3 Activity

Activity

Activity Endpoint IC50

Activity Raw

IC50 = 18.99 µM

Detail Source Activity Anticancer

Activity Measurement 18.99 µM

Target

Cancer Type Breast Cancer

Cell Line MDA-MB-435S

Tissue Affected Breast

Assay & Model

Assay MTT assay

Testing Time 24-h

Source & Reference

Database Source CancerPPD 2.0 dbACP

Source CancerPPD2

Year 2019

Evidence 4 Activity

Activity

Activity Endpoint IC50

Activity Raw

IC50 = 2.32 µM

Detail Source Activity Anticancer

Activity Measurement 2.32 µM

Target

Cancer Type Brain Tumor

Cell Line U251-MG

Tissue Affected Brain

Assay & Model

Assay MTT assay

Testing Time 24-h

Source & Reference

Database Source CancerPPD 2.0 dbACP

Source CancerPPD2

Year 2019

Evidence 5 Activity

Activity

Activity Endpoint IC50

Activity Raw

IC50 = 2.02 µM

Detail Source Activity Anticancer

Activity Measurement 2.02 µM

Target

Cancer Type Colon Cancer

Cell Line HCT-116

Tissue Affected Colon

Assay & Model

Assay MTT assay

Testing Time 24-h

Source & Reference

Database Source CancerPPD 2.0 dbACP

Source CancerPPD2

Year 2019

Evidence 6 Activity

Activity

Activity Endpoint IC50

Activity Raw

IC50 = 3.47 µM

Detail Source Activity Anticancer

Activity Measurement 3.47 µM

Target

Cancer Type Lung Cancer

Cell Line H-460

Tissue Affected Lung

Assay & Model

Assay MTT assay

Testing Time 24-h

Source & Reference

Database Source CancerPPD 2.0 dbACP

Source CancerPPD2

Year 2023

Evidence 7 Activity

Activity

Activity Endpoint IC50

Activity Raw

IC50 = 18.99 µM

Detail Source Activity Anticancer

Activity Measurement 18.99 µM

Target

Cancer Type Breast Cancer

Cell Line MDA-MB-435

Tissue Affected Breast

Assay & Model

Assay MTT assay

Testing Time 24-h

Source & Reference

Database Source CancerPPD 2.0 dbACP

Source CancerPPD2

Year 2023

Evidence 8 Activity

Activity

Activity Endpoint IC50

Activity Raw

IC50 = 2.32 µM

Detail Source Activity Anticancer

Activity Measurement 2.32 µM

Target

Cancer Type Brain Tumor

Cell Line U-251-MG

Tissue Affected Brain

Assay & Model

Assay MTT assay

Testing Time 24-h

Source & Reference

Database Source CancerPPD 2.0 dbACP

Source CancerPPD2

Year 2023

Evidence 9 Activity

Activity

Activity Endpoint IC50

Activity Raw

IC50 = 2.02 µM

Detail Source Activity Anticancer

Activity Measurement 2.02 µM

Target

Cancer Type Colon Cancer

Cell Line HCT-116

Tissue Affected Colon

Assay & Model

Assay MTT assay

Testing Time 24-h

Source & Reference

Database Source CancerPPD 2.0 dbACP

Source CancerPPD2

Year 2023

Evidence 10 Activity

Activity

Activity Raw

LDH release above 80%

Detail Source Activity Anticancer

Target

Cancer Type Colon Cancer

Cell Line HCT-116

Tissue Affected Colon

Assay & Model

Assay LDH leakage assay

Testing Time 24-h

Source & Reference

Database Source CancerPPD 2.0 dbACP

Source CancerPPD2

Year 2023

Evidence 11 Activity

Activity

Detail Source Activity Antifungal

Source & Reference

Database Source dbAMP CAFPdb Antifungipept

Other

Source Activity Label	Source Definition
Antifungal	dbAMP classified under the Anti-fungal function class.

Evidence Text

Active against S. aureus (MIC 16 mg/L), E. coli (MIC 32 mg/L), and yeast C. albicans (MIC 64 mg/L).

Evidence 12 Activity

Activity

Activity Endpoint 18% Hemolysis

Detail Source Activity Toxicity

Activity Measurement 16 µg/mL

Toxicity Type Hemolytic Cytotoxic

Toxicity Raw

18% Hemolysis | 16 | µg/mL | target cell: Horse erythrocytes

Target

Target Cell Horse erythrocytes

Source & Reference

Database Source NA

Source Database DBAASP

Reference

LFB: A Novel Antimicrobial Brevinin-Like Peptide from the Skin Secretion of the Fujian Large Headed Frog, Limnonectes fujianensi. | Biomolecules | 2019

Evidence 13 Activity

Activity

Activity Endpoint 28% Hemolysis

Detail Source Activity Toxicity

Activity Measurement 32 µg/mL

Toxicity Type Hemolytic Cytotoxic

Toxicity Raw

28% Hemolysis | 32 | µg/mL | target cell: Horse erythrocytes

Target

Target Cell Horse erythrocytes

Source & Reference

Database Source NA

Source Database DBAASP

Reference

LFB: A Novel Antimicrobial Brevinin-Like Peptide from the Skin Secretion of the Fujian Large Headed Frog, Limnonectes fujianensi. | Biomolecules | 2019

Evidence 14 Activity

Activity

Activity Endpoint 38% Hemolysis

Detail Source Activity Toxicity

Activity Measurement 64 µg/mL

Toxicity Type Hemolytic Cytotoxic

Toxicity Raw

38% Hemolysis | 64 | µg/mL | target cell: Horse erythrocytes

Target

Target Cell Horse erythrocytes

Source & Reference

Database Source NA

Source Database DBAASP

Reference

LFB: A Novel Antimicrobial Brevinin-Like Peptide from the Skin Secretion of the Fujian Large Headed Frog, Limnonectes fujianensi. | Biomolecules | 2019

Evidence 15 Activity

Activity

Activity Endpoint 60% Hemolysis

Detail Source Activity Toxicity

Activity Measurement 128 µg/mL

Toxicity Type Hemolytic Cytotoxic

Toxicity Raw

60% Hemolysis | 128 | µg/mL | target cell: Horse erythrocytes

Target

Target Cell Horse erythrocytes

Source & Reference

Database Source NA

Source Database DBAASP

Reference

LFB: A Novel Antimicrobial Brevinin-Like Peptide from the Skin Secretion of the Fujian Large Headed Frog, Limnonectes fujianensi. | Biomolecules | 2019

Evidence 16 Activity

Activity

Activity Endpoint 95% Hemolysis

Detail Source Activity Toxicity

Activity Measurement 256 µg/mL

Toxicity Type Hemolytic Cytotoxic

Toxicity Raw

95% Hemolysis | 256 | µg/mL | target cell: Horse erythrocytes

Target

Target Cell Horse erythrocytes

Source & Reference

Database Source NA

Source Database DBAASP

Reference

LFB: A Novel Antimicrobial Brevinin-Like Peptide from the Skin Secretion of the Fujian Large Headed Frog, Limnonectes fujianensi. | Biomolecules | 2019

Evidence 17 Activity

Activity

Activity Endpoint 100% Hemolysis

Detail Source Activity Toxicity

Activity Measurement 512 µg/mL

Toxicity Type Hemolytic Cytotoxic

Toxicity Raw

100% Hemolysis | 512 | µg/mL | target cell: Horse erythrocytes

Target

Target Cell Horse erythrocytes

Source & Reference

Database Source NA

Source Database DBAASP

Reference

LFB: A Novel Antimicrobial Brevinin-Like Peptide from the Skin Secretion of the Fujian Large Headed Frog, Limnonectes fujianensi. | Biomolecules | 2019

Evidence 18 Activity

Activity

Detail Source Activity Toxicity

Toxicity Type Hemolytic Cytotoxic

Toxicity Raw

Hemolytic_activity: Not available | Cytotoxicity: Not available

Target

Target Cell erythrocytes

Source & Reference

Database Source NA

Source Database DRAMP

Reference

Biomolecules. 2019 Jun 21 9(6):242

Evidence 19 Hemolysis

Source & Reference

source PD_Hemolysis

Database Source PD_Hemolysis