TPDB

3D PDB MODEL

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TPDB ID TPDB13490

Activity

Antibacterial Hemolysis Toxicity

Activity_category_statistics

Anti-infective peptides Toxicity and safety peptides

Database_Source

AntiBP3 Peptipedia PD_Hemolysis

Sequence_Type

standard

Has_Modification No

Description A 25-aa standard natural multi-activity (Antibacterial, Hemolysis, and Toxicity) peptide sequence curated from AntiBP3, Peptipedia, and PD_Hemolysis, with an available 3D structural model.

Sequence

AGTNRIAKELRRYIRKVGRIARKKW

FASTA

>TPDB13490|Antibacterial;Hemolysis;Toxicity|length=25
AGTNRIAKELRRYIRKVGRIARKKW

Formula C135H235N49O31

Absent Amino Acids DCQHMFPS

Common Amino Acids R

Mass 3040.66

PI 12.42

Basic Residues 10

Acidic Residues 1

Hydrophobic Residues 10

Net Charge +9

Boman Index 6.70

Hydrophobicity -1.056

Aliphatic Index 86.00

Half Life

Mammalian: 4.4 hour Yeast: >20 hour E.coli: >10 hour

Extinction Coefficient Cystines 6990

Absorbance 280nm 229.88

Polar Residues 3

number

3

A

6

R

1

N

0

D

0

C

1

E

0

Q

2

G

0

H

3

I

1

L

4

K

0

M

0

F

0

P

0

S

1

T

1

W

1

Y

1

V

Amino Acid Distribution

A: 3 R: 6 N: 1 D: 0 C: 0 E: 1 Q: 0 G: 2 H: 0 I: 3 L: 1 K: 4 M: 0 F: 0 P: 0 S: 0 T: 1 W: 1 Y: 1 V: 1

Length

25

Formula

C135H235N49O31

Mass

3040.66

PI

12.42

Net Charge

+9

Boman Index

6.70

Hydrophobicity

-1.056

Evidence 1 Activity

Activity

Activity Endpoint 11.7% Hemolysis

Detail Source Activity Toxicity

Activity Measurement >2 µM

Toxicity Type Hemolytic Cytotoxic

Toxicity Raw

11.7% Hemolysis | >2 | µM | target cell: Sheep erythrocytes

Target

Target Cell Sheep erythrocytes

Source & Reference

Database Source NA

Source Database DBAASP

Reference

Synthetic Peptide Libraries Designed From a Minimal Alpha-Helical Domain of AS-48-Bacteriocin Homologs Exhibit Potent Antibacterial Activity. | Front Microbiol | 2020

Evidence 2 Activity

Activity

Activity Endpoint 7% Cytotoxicity

Detail Source Activity Toxicity

Activity Measurement 32 µM

Toxicity Type Cytotoxic

Toxicity Raw

7% Cytotoxicity | 32 | µM | target cell: Human keratinocytes HaCat

Target

Target Cell Human keratinocytes HaCat

Source & Reference

Database Source NA

Source Database DBAASP

Reference

Synthetic Peptide Libraries Designed From a Minimal Alpha-Helical Domain of AS-48-Bacteriocin Homologs Exhibit Potent Antibacterial Activity. | Front Microbiol | 2020

Evidence 3 Activity

Activity

Activity Endpoint 32% Cytotoxicity

Detail Source Activity Toxicity

Activity Measurement 64 µM

Toxicity Type Cytotoxic

Toxicity Raw

32% Cytotoxicity | 64 | µM | target cell: Human keratinocytes HaCat

Target

Target Cell Human keratinocytes HaCat

Source & Reference

Database Source NA

Source Database DBAASP

Reference

Synthetic Peptide Libraries Designed From a Minimal Alpha-Helical Domain of AS-48-Bacteriocin Homologs Exhibit Potent Antibacterial Activity. | Front Microbiol | 2020

Evidence 4 Hemolysis

Source & Reference

source PD_Hemolysis

Database Source PD_Hemolysis