TPDB

3D PDB MODEL

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TPDB ID TPDB13299

Activity

Antibacterial Antifungal Hemolysis Toxicity

Activity_category_statistics

Anti-infective peptides Toxicity and safety peptides

Database_Source

AntiBP3 dbAMP DRAMP Peptipedia Antifungipept CAFPdb PD_Hemolysis

Sequence_Type

standard

Has_Modification No

Description A 21-aa standard natural multi-activity (Antibacterial, Antifungal, Hemolysis, and other sources) peptide sequence curated from AntiBP3, dbAMP, DRAMP, and other sources, with an available 3D structural model.

Sequence

LLAGLAANFLPKIFCKITRKC

FASTA

>TPDB13299|Antibacterial;Antifungal;Hemolysis;Toxicity|length=21
LLAGLAANFLPKIFCKITRKC

Formula C108H182N28O24S2

Absent Amino Acids DEQHMSWYV

Common Amino Acids L

Mass 2320.93

PI 10.32

Basic Residues 4

Acidic Residues 0

Hydrophobic Residues 11

Net Charge +4

Boman Index -1.31

Hydrophobicity 0.848

Aliphatic Index 125.71

Half Life

Mammalian: 5.5 hour Yeast: 3 min E.coli: 2 min

Extinction Coefficient Cystines 125

Absorbance 280nm 5.39

Polar Residues 4

number

3

A

1

R

1

N

0

D

2

C

0

E

0

Q

1

G

0

H

2

I

4

L

3

K

0

M

2

F

1

P

0

S

1

T

0

W

0

Y

0

V

Amino Acid Distribution

A: 3 R: 1 N: 1 D: 0 C: 2 E: 0 Q: 0 G: 1 H: 0 I: 2 L: 4 K: 3 M: 0 F: 2 P: 1 S: 0 T: 1 W: 0 Y: 0 V: 0

Length

21

Formula

C108H182N28O24S2

Mass

2320.93

PI

10.32

Net Charge

+4

Boman Index

-1.31

Hydrophobicity

0.848

Evidence 1 Activity

Activity

Activity Endpoint HD50

Detail Source Activity Hemolysis

Activity Measurement 77 µg/mL

Hemolysis Raw

[Ref.9748603]HD50=77 µg/ml(oxidized) HD50=110 µg/ml(reduced) against mouse red blood cells

Target

RBC Target mouse red blood cells

Source & Reference

Database Source Hemolytik PD_Hemolysis

Source DRAMP4

Reference

Biochim Biophys Acta. 1998 Sep 8 1387(1-2):239-48.

Other

Source Activity Label	Source Definition
Hemolysis	Hemolytik experimentally validated hemolytic peptide annotation.

Evidence 2 Activity

Activity

Activity Endpoint 50% Hemolysis

Detail Source Activity Toxicity

Activity Measurement 77 µg/mL

Toxicity Type Hemolytic Cytotoxic

Toxicity Raw

50% Hemolysis | 77 | µg/mL | target cell: Mouse erythrocytes

Target

Target Cell Mouse erythrocytes

Source & Reference

Database Source NA

Source Database DBAASP

Reference

Structure-activity analysis of brevinin 1E amide, an antimicrobial peptide from Rana esculenta. | Biochim Biophys Acta | 1998

Evidence 3 Activity

Activity

Activity Endpoint 50% Hemolysis

Detail Source Activity Toxicity

Activity Measurement 110 µg/mL

Toxicity Type Hemolytic Cytotoxic

Toxicity Raw

50% Hemolysis | 110 | µg/mL | target cell: Mouse erythrocytes

Target

Target Cell Mouse erythrocytes

Source & Reference

Database Source NA

Source Database DBAASP

Reference

Structure-activity analysis of brevinin 1E amide, an antimicrobial peptide from Rana esculenta. | Biochim Biophys Acta | 1998

Evidence 4 Activity

Activity

Activity Endpoint HD50

Detail Source Activity Toxicity

Activity Measurement 77 µg/mL

Toxicity Type Hemolytic

Toxicity Raw

Hemolytic_activity: [Ref.9748603]HD50=77 µg/ml(oxidized) HD50=110 µg/ml(reduced) against mouse red blood cells

Target

Target Cell erythrocytes

Source & Reference

Database Source NA

Source Database DRAMP

Reference

Biochim Biophys Acta. 1998 Sep 8 1387(1-2):239-48.

Evidence 5 Hemolysis

Activity

Activity Measurement 77 µg/mL

Activity Endpoint HD50

Hemolysis Raw

[Ref.9748603]HD50=77 µg/ml(oxidized) HD50=110 µg/ml(reduced) against mouse red blood cells

Detail Source Activity Hemolysis

Target

RBC Target mouse red blood cells

Source & Reference

source PD_Hemolysis

Reference

Biochim Biophys Acta. 1998 Sep 8 1387(1-2):239-48.

Source DRAMP4

Database Source PD_Hemolysis