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Peptide record

TPDB13268

Brevinin-1H Antibacterial Anticancer Antifungal Hemolysis Toxicity standard

21 amino acids

3D PDB MODEL

Drag to rotate. Click a residue or atom to inspect it; the selected residue is highlighted in amber.

TPDB ID TPDB13268

Peptide_Name

Brevinin-1H

Activity

Antibacterial Anticancer Antifungal Hemolysis Toxicity

Activity_category_statistics

Anti-infective peptides Cancer-related peptides Toxicity and safety peptides

Database_Source

AntiBP3 Peptipedia CancerPPD 2.0 dbACP CAFPdb dbAMP Hemolytic2 PD_Hemolysis ToxinPred 3.0

Sequence_Type

standard

Has_Modification No

Description A 21-aa standard natural multi-activity (Antibacterial, Anticancer, Antifungal, and other sources) peptide sequence curated from AntiBP3, Peptipedia, CancerPPD 2.0, and other sources, with an available 3D structural model.

Sequence

FALGAVTKVLPKLFCLITRKC

FASTA

>TPDB13268|Antibacterial;Anticancer;Antifungal;Hemolysis;Toxicity|length=21
FALGAVTKVLPKLFCLITRKC

Formula C109H185N27O24S2

Absent Amino Acids NDEQHMSWY

Common Amino Acids L

Mass 2321.95

PI 10.32

Basic Residues 4

Acidic Residues 0

Hydrophobic Residues 11

Net Charge +4

Boman Index -0.97

Hydrophobicity 1.081

Aliphatic Index 130.00

Half Life

Mammalian: 1.1 hour Yeast: 3 min E.coli: 2 min

Extinction Coefficient Cystines 125

Absorbance 280nm 5.38

Polar Residues 4

number

Amino Acid Distribution

A: 2 R: 1 N: 0 D: 0 C: 2 E: 0 Q: 0 G: 1 H: 0 I: 1 L: 4 K: 3 M: 0 F: 2 P: 1 S: 0 T: 2 W: 0 Y: 0 V: 2

Length

Formula

C109H185N27O24S2

Mass

2321.95

10.32

Net Charge

Boman Index

-0.97

Hydrophobicity

1.081

C_terminal_Modification

Free

Chirality

Linear_or_Cyclic

Linear

N_terminal_Modification

Free

Nature

Antimicrobial

Origin

Amolops hainanensis

Evidence 1 Activity

Activity

Activity Endpoint MIC

Detail Source Activity Antibacterial

Activity Measurement 4 µM

Target

Target Organism Staphylococcus aureus

Source & Reference

Database Source dbAMP

Other

Source Activity Label	Source Definition
Antibacterial	dbAMP classified as Antibacterial under the Anti-bacterial function class.

Evidence Text

Active against Gram+ S. aureus (MIC 4 uM), MRSA (MIC 8 uM), S. aureus biofilms, Gram- E. coli (MIC 16 uM), P. aeruginosa (MIC 64 uM

Evidence 2 Activity

Activity

Activity Endpoint MIC

Detail Source Activity Antibacterial

Activity Measurement 16 µM

Target

Target Organism Escherichia coli

Source & Reference

Database Source dbAMP

Other

Evidence Text

Active against Gram+ S. aureus (MIC 4 uM), MRSA (MIC 8 uM), S. aureus biofilms, Gram- E. coli (MIC 16 uM), P. aeruginosa (MIC 64 uM), K. pneumoniae (MIC 64 uM), and yeast C. albicans (MIC 16 uM).

Evidence 3 Activity

Activity

Activity Endpoint MIC

Detail Source Activity Antibacterial

Activity Measurement 64 µM

Target

Target Organism Pseudomonas aeruginosa

Source & Reference

Database Source dbAMP

Other

Evidence Text

am+ S. aureus (MIC 4 uM), MRSA (MIC 8 uM), S. aureus biofilms, Gram- E. coli (MIC 16 uM), P. aeruginosa (MIC 64 uM), K. pneumoniae (MIC 64 uM), and yeast C. albicans (MIC 16 uM). MBC values are rather simi

Evidence 4 Activity

Activity

Activity Endpoint MIC

Detail Source Activity Antibacterial

Activity Measurement 64 µM

Target

Target Organism Klebsiella pneumoniae

Source & Reference

Database Source dbAMP

Other

Evidence Text

RSA (MIC 8 uM), S. aureus biofilms, Gram- E. coli (MIC 16 uM), P. aeruginosa (MIC 64 uM), K. pneumoniae (MIC 64 uM), and yeast C. albicans (MIC 16 uM). MBC values are rather similar to MIC (equal or 2 fold

Evidence 5 Activity

Activity

Activity Endpoint MIC

Detail Source Activity Antibacterial

Activity Measurement 16 µM

Target

Target Organism Candida albicans

Source & Reference

Database Source dbAMP

Other

Evidence Text

ram- E. coli (MIC 16 uM), P. aeruginosa (MIC 64 uM), K. pneumoniae (MIC 64 uM), and yeast C. albicans (MIC 16 uM). MBC values are rather similar to MIC (equal or 2 fold hihger). This parent peptide can a

Evidence 6 Activity

Activity

Activity Endpoint IC50

Activity Raw

IC50 = 5.87 µM

Detail Source Activity Anticancer

Activity Measurement 5.87 µM

Target

Cancer Type Prostate Cancer

Cell Line PC-3

Tissue Affected Prostate

Assay & Model

Assay MTT assay

Testing Time 24-h

Source & Reference

Database Source CancerPPD 2.0 dbACP

Source CancerPPD2

Year 2021

Other

Source Activity Label	Source Definition
Anticancer	CancerPPD 2.0 manually curated experimentally verified anticancer peptide/protein annotation.

Evidence 7 Activity

Activity

Activity Endpoint IC50

Activity Raw

IC50 = 3.47 µM

Detail Source Activity Anticancer

Activity Measurement 3.47 µM

Target

Cancer Type Breast Cancer

Cell Line MDA-MB-435S

Tissue Affected Breast

Assay & Model

Assay MTT assay

Testing Time 24-h

Source & Reference

Database Source CancerPPD 2.0 dbACP

Source CancerPPD2

Year 2021

Evidence 8 Activity

Activity

Activity Endpoint IC50

Activity Raw

IC50 = 3.37 µM

Detail Source Activity Anticancer

Activity Measurement 3.37 µM

Target

Cancer Type Lung Cancer

Cell Line H-157

Tissue Affected Lung

Assay & Model

Assay MTT assay

Testing Time 24-h

Source & Reference

Database Source CancerPPD 2.0 dbACP

Source CancerPPD2

Year 2021

Evidence 9 Activity

Activity

Activity Endpoint IC50

Activity Raw

IC50 = 2.599 µM

Detail Source Activity Anticancer

Activity Measurement 2.599 µM

Target

Cancer Type Colon Cancer

Cell Line HCT-116

Tissue Affected Colon

Assay & Model

Assay MTT assay

Testing Time 24-h

Source & Reference

Database Source CancerPPD 2.0 dbACP

Source CancerPPD2

Year 2021

Evidence 10 Activity

Activity

Activity Endpoint MIC

Detail Source Activity Antifungal

Activity Measurement 4 µM

Target

Target Organism Staphylococcus aureus

Source & Reference

Database Source dbAMP CAFPdb

Other

Source Activity Label	Source Definition
Antifungal	dbAMP classified under the Anti-fungal function class.

Evidence Text

Active against Gram+ S. aureus (MIC 4 uM), MRSA (MIC 8 uM), S. aureus biofilms, Gram- E. coli (MIC 16 uM), P. aeruginosa (MIC 64 uM

Evidence 11 Activity

Activity

Activity Endpoint MIC

Detail Source Activity Antifungal

Activity Measurement 16 µM

Target

Target Organism Escherichia coli

Source & Reference

Database Source dbAMP CAFPdb

Other

Evidence Text

Active against Gram+ S. aureus (MIC 4 uM), MRSA (MIC 8 uM), S. aureus biofilms, Gram- E. coli (MIC 16 uM), P. aeruginosa (MIC 64 uM), K. pneumoniae (MIC 64 uM), and yeast C. albicans (MIC 16 uM).

Evidence 12 Activity

Activity

Activity Endpoint MIC

Detail Source Activity Antifungal

Activity Measurement 64 µM

Target

Target Organism Pseudomonas aeruginosa

Source & Reference

Database Source dbAMP CAFPdb

Other

Evidence Text

Evidence 13 Activity

Activity

Activity Endpoint MIC

Detail Source Activity Antifungal

Activity Measurement 64 µM

Target

Target Organism Klebsiella pneumoniae

Source & Reference

Database Source dbAMP CAFPdb

Other

Evidence Text

Evidence 14 Activity

Activity

Activity Endpoint MIC

Detail Source Activity Antifungal

Activity Measurement 16 µM

Target

Target Organism Candida albicans

Source & Reference

Database Source dbAMP CAFPdb

Other

Evidence Text

Evidence 15 Activity

Activity

Activity Endpoint 9.49% Hemolysis

Detail Source Activity Toxicity

Activity Measurement 4 µM

Toxicity Type Hemolytic Cytotoxic

Toxicity Raw

9.49% Hemolysis | 4 | µM | target cell: Horse erythrocytes

Target

Target Cell Horse erythrocytes

Source & Reference

Database Source NA

Source Database DBAASP

Reference

Characterisation of a novel peptide, Brevinin-1H, from the skin secretion of Amolops hainanensis and rational design of several analogues. | Chem Biol Drug Des | 2020

Evidence 16 Activity

Activity

Activity Endpoint 28% Hemolysis

Detail Source Activity Toxicity

Activity Measurement 32 µM

Toxicity Type Hemolytic Cytotoxic

Toxicity Raw

28% Hemolysis | 32 | µM | target cell: Horse erythrocytes

Target

Target Cell Horse erythrocytes

Source & Reference

Database Source NA

Source Database DBAASP

Reference

Characterisation of a novel peptide, Brevinin-1H, from the skin secretion of Amolops hainanensis and rational design of several analogues. | Chem Biol Drug Des | 2020

Evidence 17 Activity

Activity

Activity Endpoint 50% Hemolysis

Detail Source Activity Toxicity

Activity Measurement 53.12 µM

Toxicity Type Hemolytic Cytotoxic

Toxicity Raw

50% Hemolysis | 53.12 | µM | target cell: Horse erythrocytes

Target

Target Cell Horse erythrocytes

Source & Reference

Database Source NA

Source Database DBAASP

Reference

Characterisation of a novel peptide, Brevinin-1H, from the skin secretion of Amolops hainanensis and rational design of several analogues. | Chem Biol Drug Des | 2020

Evidence 18 Activity

Activity

Activity Endpoint HC50

Detail Source Activity Toxicity

Activity Measurement 53.12 µM

Toxicity Type Hemolytic Cytotoxic

Toxicity Raw

Hemolytic_activity: Not available | Cytotoxicity: Horse blood cells: HC50=53.12 µM

Target

Target Cell erythrocytes

Source & Reference

Database Source NA

Source Database DRAMP

Reference

Chem Biol Drug Des. 2021 Feb 97(2):273-282.

Evidence 19 Hemolysis

Source & Reference

source PD_Hemolysis

Database Source PD_Hemolysis