TPDB

3D PDB MODEL

Drag to rotate. Click a residue or atom to inspect it; the selected residue is highlighted in amber.

TPDB ID TPDB13188

Activity

Antibacterial Hemolysis Toxicity

Activity_category_statistics

Anti-infective peptides Toxicity and safety peptides

Database_Source

AntiBP3 dbAMP DRAMP Peptipedia HemoPI Hemolytic2 PD_Hemolysis

Sequence_Type

standard

Has_Modification No

Description A 19-aa standard natural multi-activity (Antibacterial, Hemolysis, and Toxicity) peptide sequence curated from AntiBP3, dbAMP, DRAMP, and other sources, with an available 3D structural model.

Sequence

KFAKKFKWFAKAAFKFFKK

FASTA

>TPDB13188|Antibacterial;Hemolysis;Toxicity|length=19
KFAKKFKWFAKAAFKFFKK

Formula C125H182N28O20

Absent Amino Acids RNDCEQGHILMPSTYV

Common Amino Acids K

Mass 2397.00

PI 11.36

Basic Residues 8

Acidic Residues 0

Hydrophobic Residues 11

Net Charge +8

Boman Index -1.39

Hydrophobicity -0.426

Aliphatic Index 21.05

Half Life

Mammalian: 1.3 hour Yeast: 3 min E.coli: 2 min

Extinction Coefficient Cystines 5500

Absorbance 280nm 229.45

Polar Residues 0

number

4

A

0

R

0

N

0

D

0

C

0

E

0

Q

0

G

0

H

0

I

0

L

8

K

0

M

6

F

0

P

0

S

0

T

1

W

0

Y

0

V

Amino Acid Distribution

A: 4 R: 0 N: 0 D: 0 C: 0 E: 0 Q: 0 G: 0 H: 0 I: 0 L: 0 K: 8 M: 0 F: 6 P: 0 S: 0 T: 0 W: 1 Y: 0 V: 0

Length

19

Formula

C125H182N28O20

Mass

2397.00

PI

11.36

Net Charge

+8

Boman Index

-1.39

Hydrophobicity

-0.426

Evidence 1 Activity

Activity

Activity Endpoint EC50

Detail Source Activity Hemolysis

Activity Measurement 195 µg/mL

Hemolysis Raw

[Ref.23019445]EC50=195 µg/mL against human red blood cells.

Target

RBC Target human red blood cells

Source & Reference

Database Source Hemolytik PD_Hemolysis

Source DRAMP4

Reference

Beilstein J Org Chem. 2012 8:1172-84. Epub 2012 Jul 24.

Other

Source Activity Label	Source Definition
Hemolysis	Hemolytik experimentally validated hemolytic peptide annotation.

Evidence 2 Activity

Activity

Activity Endpoint 50% Hemolysis

Detail Source Activity Toxicity

Activity Measurement 195 µg/mL

Toxicity Type Hemolytic Cytotoxic

Toxicity Raw

50% Hemolysis | 195 | µg/mL | target cell: Human erythrocytes

Target

Target Cell Human erythrocytes

Source & Reference

Database Source NA

Source Database DBAASP

Evidence 3 Activity

Activity

Activity Endpoint EC50

Detail Source Activity Toxicity

Activity Measurement 195 µg/mL

Toxicity Type Hemolytic

Toxicity Raw

Hemolytic_activity: [Ref.23019445]EC50=195 µg/mL against human red blood cells.

Target

Target Cell erythrocytes

Source & Reference

Database Source NA

Source Database DRAMP

Reference

Beilstein J Org Chem. 2012 8:1172-84. Epub 2012 Jul 24.

Evidence 4 Hemolysis

Activity

Activity Measurement 195 µg/mL

Activity Endpoint EC50

Hemolysis Raw

[Ref.23019445]EC50=195 µg/mL against human red blood cells.

Detail Source Activity Hemolysis

Target

RBC Target human red blood cells

Source & Reference

source HemoPI PD_Hemolysis

Reference

Beilstein J Org Chem. 2012 8:1172-84. Epub 2012 Jul 24.

Source DRAMP4

Database Source PD_Hemolysis