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Peptide record

TPDB12599

Antibacterial Toxicity standard
13 amino acids
Basic Information
3D PDB MODEL
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TPDB12599
Antibacterial Toxicity
Anti-infective peptides Toxicity and safety peptides
AntiBP3 DRAMP Peptipedia
standard
No
A 13-aa standard natural multi-activity (Antibacterial and Toxicity) peptide sequence curated from AntiBP3, DRAMP, and Peptipedia, with an available 3D structural model.
Sequence
KIAKRIWKILRRR
Physicochemical Analysis
C80H145N29O14
NDCEQGHMFPSTYV
R
1737.22
12.88
7
0
6
+7
2.70
-0.885
127.69
Mammalian: 1.3 hour Yeast: 3 min E.coli: 2 min
5500
316.60
0
Residue Composition
number
1
A
4
R
0
N
0
D
0
C
0
E
0
Q
0
G
0
H
3
I
1
L
3
K
0
M
0
F
0
P
0
S
0
T
1
W
0
Y
0
V
Amino Acid Distribution
A: 1 R: 4 N: 0 D: 0 C: 0 E: 0 Q: 0 G: 0 H: 0 I: 3 L: 1 K: 3 M: 0 F: 0 P: 0 S: 0 T: 0 W: 1 Y: 0 V: 0
Chemical Descriptors
13
C80H145N29O14
1737.22
12.88
+7
2.70
-0.885
Evidence Records 3 records
Evidence 1 Activity

Activity

10% Hemolysis
Toxicity
125 µg/mL
Hemolytic Cytotoxic
10% Hemolysis | 125 | µg/mL | target cell: Human erythrocytes

Target

Human erythrocytes

Source & Reference

NA
DBAASP
A novel, rationally designed, hybrid antimicrobial peptide, inspired by cathelicidin and aurein, exhibits membrane-active mechanisms against Pseudomonas aeruginosa. | Sci Rep | 2020
Evidence 2 Activity

Activity

10% Killing
Toxicity
7.81-62.5 µg/mL
Cytotoxic
10% Killing | 7.81-62.5 | µg/mL | target cell: Mouse fibroblasts L929

Target

Mouse fibroblasts L929

Source & Reference

NA
DBAASP
A novel, rationally designed, hybrid antimicrobial peptide, inspired by cathelicidin and aurein, exhibits membrane-active mechanisms against Pseudomonas aeruginosa. | Sci Rep | 2020
Evidence 3 Activity

Activity

MHC
Toxicity
125 µg/mL
Hemolytic Cytotoxic
Hemolytic_activity: [Ref.32499514]MHC = 125 µg/mL. Note:MHC is the minimum concentration that caused 10% hemolysis of human red blood cells (hRBC). When no 10% hemolysis was observed at 250 µg/ml, a value of 500 µg/ml was used to calculate the therapeutic index. | Cytotoxicity: [Ref.32499514]Cytotoxic activity of PA-13 against the L929 mouse fibroblast cell line was determined by a colorimetric MTT viability assay. At the MIC (3.91 µg/ml), the cell survival was up to 90%. Even at the maximum concentration (250 µg/ml), the survival was common (81.29%). Melittin, used as a positive control, showed a strong and dose-dependent cytotoxic effect no living cells were detected at 62.5 µg/ml. Peptide PA-13 displayed very little cytotoxicity on L929 cells when compared to melittin.

Target

erythrocytes

Source & Reference

NA
DRAMP
Sci Rep. 2020 Jun 4 10(1):9117. doi: 10.1038/s41598-020-65688-5.