TPDB

3D PDB MODEL

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TPDB ID TPDB12548

Activity

Antibacterial Hemolysis Toxicity

Activity_category_statistics

Anti-infective peptides Toxicity and safety peptides

Database_Source

AntiBP3 dbAMP DRAMP Peptipedia HemoPI Hemolytic2 PD_Hemolysis

Sequence_Type

standard

Has_Modification No

Description A 12-aa standard natural multi-activity (Antibacterial, Hemolysis, and Toxicity) peptide sequence curated from AntiBP3, dbAMP, DRAMP, and other sources, with an available 3D structural model.

Sequence

IWRIFRRIFRIF

FASTA

>TPDB12548|Antibacterial;Hemolysis;Toxicity|length=12
IWRIFRRIFRIF

Formula C86H131N25O13

Absent Amino Acids ANDCEQGHLKMPSTYV

Common Amino Acids RI

Mass 1723.15

PI 12.88

Basic Residues 4

Acidic Residues 0

Hydrophobic Residues 8

Net Charge +4

Boman Index -3.24

Hydrophobicity 0.625

Aliphatic Index 130.00

Half Life

Mammalian: 20 hour Yeast: 30 min E.coli: >10 hour

Extinction Coefficient Cystines 5500

Absorbance 280nm 319.18

Polar Residues 0

number

0

A

4

R

0

N

0

D

0

C

0

E

0

Q

0

G

0

H

4

I

0

L

0

K

0

M

3

F

0

P

0

S

0

T

1

W

0

Y

0

V

Amino Acid Distribution

A: 0 R: 4 N: 0 D: 0 C: 0 E: 0 Q: 0 G: 0 H: 0 I: 4 L: 0 K: 0 M: 0 F: 3 P: 0 S: 0 T: 0 W: 1 Y: 0 V: 0

Length

12

Formula

C86H131N25O13

Mass

1723.15

PI

12.88

Net Charge

+4

Boman Index

-3.24

Hydrophobicity

0.625

Evidence 1 Activity

Activity

Activity Endpoint MHC

Detail Source Activity Hemolysis

Activity Measurement 19.6 µM

Hemolysis Raw

[Ref.22699557]MHC=19.6 µM against human red blood cells

Target

RBC Target human red blood cells

Source & Reference

Database Source Hemolytik PD_Hemolysis

Source DRAMP4

Reference

Amino Acids. 2012 Dec 43(6):2527-36. doi: 10.1007/s00726-012-1334-7.

Other

Source Activity Label	Source Definition
Hemolysis	Hemolytik experimentally validated hemolytic peptide annotation.

Evidence 2 Activity

Activity

Activity Endpoint 50% Hemolysis

Detail Source Activity Toxicity

Activity Measurement 19.6 µM

Toxicity Type Hemolytic Cytotoxic

Toxicity Raw

50% Hemolysis | 19.6 | µM | target cell: Human erythrocytes

Target

Target Cell Human erythrocytes

Source & Reference

Database Source NA

Source Database DBAASP

Reference

Biochemical property and membrane-peptide interactions of de novo antimicrobial peptides designed by helix-forming units. | Amino Acids | 2012

Evidence 3 Activity

Activity

Activity Endpoint MHC

Detail Source Activity Toxicity

Activity Measurement 19.6 µM

Toxicity Type Hemolytic

Toxicity Raw

Hemolytic_activity: [Ref.22699557]MHC=19.6 µM against human red blood cells

Target

Target Cell erythrocytes

Source & Reference

Database Source NA

Source Database DRAMP

Reference

Amino Acids. 2012 Dec 43(6):2527-36. doi: 10.1007/s00726-012-1334-7.

Evidence 4 Hemolysis

Activity

Activity Measurement 19.6 µM

Activity Endpoint MHC

Hemolysis Raw

[Ref.22699557]MHC=19.6 µM against human red blood cells

Detail Source Activity Hemolysis

Target

RBC Target human red blood cells

Source & Reference

source HemoPI PD_Hemolysis

Reference

Amino Acids. 2012 Dec 43(6):2527-36. doi: 10.1007/s00726-012-1334-7.

Source DRAMP4

Database Source PD_Hemolysis