TPDB

3D PDB MODEL

Drag to rotate. Click a residue or atom to inspect it; the selected residue is highlighted in amber.

TPDB ID TPDB12518

Activity

Antibacterial Hemolysis Toxicity

Activity_category_statistics

Anti-infective peptides Toxicity and safety peptides

Database_Source

AntiBP3 dbAMP DRAMP Peptipedia PD_Hemolysis

Sequence_Type

standard

Has_Modification No

Description A 12-aa standard natural multi-activity (Antibacterial, Hemolysis, and Toxicity) peptide sequence curated from AntiBP3, dbAMP, DRAMP, and other sources, with an available 3D structural model.

Sequence

KKLFKKILKYLA

FASTA

>TPDB12518|Antibacterial;Hemolysis;Toxicity|length=12
KKLFKKILKYLA

Formula C75H129N17O14

Absent Amino Acids RNDCEQGHMPSTWV

Common Amino Acids K

Mass 1492.95

PI 10.77

Basic Residues 5

Acidic Residues 0

Hydrophobic Residues 7

Net Charge +5

Boman Index 0.72

Hydrophobicity -0.025

Aliphatic Index 138.33

Half Life

Mammalian: 1.3 hour Yeast: 3 min E.coli: 2 min

Extinction Coefficient Cystines 1490

Absorbance 280nm 99.80

Polar Residues 1

number

1

A

0

R

0

N

0

D

0

C

0

E

0

Q

0

G

0

H

1

I

3

L

5

K

0

M

1

F

0

P

0

S

0

T

0

W

1

Y

0

V

Amino Acid Distribution

A: 1 R: 0 N: 0 D: 0 C: 0 E: 0 Q: 0 G: 0 H: 0 I: 1 L: 3 K: 5 M: 0 F: 1 P: 0 S: 0 T: 0 W: 0 Y: 1 V: 0

Length

12

Formula

C75H129N17O14

Mass

1492.95

PI

10.77

Net Charge

+5

Boman Index

0.72

Hydrophobicity

-0.025

Evidence 1 Activity

Activity

Detail Source Activity Hemolysis

Hemolysis Raw

[Ref.29750913] C50 =6.3µM against human red blood cells

Target

RBC Target human red blood cells

Source & Reference

Database Source Hemolytik PD_Hemolysis

Source DRAMP4

Reference

Biochim Biophys Acta. 2018 Aug 1860(8):1502-1516.

Other

Source Activity Label	Source Definition
Hemolysis	Hemolytik experimentally validated hemolytic peptide annotation.

Evidence 2 Activity

Activity

Activity Endpoint 50% Hemolysis

Detail Source Activity Toxicity

Activity Measurement 6.3 µM

Toxicity Type Hemolytic Cytotoxic

Toxicity Raw

50% Hemolysis | 6.3 | µM | target cell: Human erythrocytes

Target

Target Cell Human erythrocytes

Source & Reference

Database Source NA

Source Database DBAASP

Reference

Synthesis, biophysical and functional studies of two BP100 analogues modified by a hydrophobic chain and a cyclic peptide. | Biochim Biophys Acta | 2018

Evidence 3 Activity

Activity

Detail Source Activity Toxicity

Toxicity Type Hemolytic

Toxicity Raw

Hemolytic_activity: [Ref.29750913] C50 =6.3µM against human red blood cells

Target

Target Cell erythrocytes

Source & Reference

Database Source NA

Source Database DRAMP

Reference

Biochim Biophys Acta. 2018 Aug 1860(8):1502-1516.

Evidence 4 Hemolysis

Activity

Hemolysis Raw

[Ref.29750913] C50 =6.3µM against human red blood cells

Detail Source Activity Hemolysis

Target

RBC Target human red blood cells

Source & Reference

source PD_Hemolysis

Reference

Biochim Biophys Acta. 2018 Aug 1860(8):1502-1516.

Source DRAMP4

Database Source PD_Hemolysis