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Peptide record

TPDB12309

(KFF)3K (KFF)3K (BPPS) (KFF)3K-anti-gyrA PNA/scPNA CPP CPP-PNA 12-1 CPP-PNA scramble Antibacterial Antifungal Cell-penetrating Peptides Toxicity standard
10 amino acids
Basic Information
3D PDB MODEL
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TPDB12309
(KFF)3K (KFF)3K (BPPS) (KFF)3K-anti-gyrA PNA/scPNA CPP CPP-PNA 12-1 CPP-PNA scramble
Antibacterial Antifungal Cell-penetrating Peptides Toxicity
Anti-infective peptides Toxicity and safety peptides Delivery and barrier-penetrating peptides
AntiBP3 Peptipedia CellPPD-MOD CPPsite3.0 MLCPP 2.0
standard
No
A 10-aa standard natural multi-activity (Antibacterial, Antifungal, Cell-penetrating Peptides, and other sources) peptide sequence curated from AntiBP3, Peptipedia, CellPPD-MOD, and other sources, with an available 3D structural model.
Sequence
KFFKFFKFFK
Physicochemical Analysis
C78H104N14O11
ARNDCEQGHILMPSTWYV
F
1413.77
11.00
4
0
6
+4
-2.82
0.120
0.00
Mammalian: 1.3 hour Yeast: 3 min E.coli: 2 min
0
0.00
0
Residue Composition
number
0
A
0
R
0
N
0
D
0
C
0
E
0
Q
0
G
0
H
0
I
0
L
4
K
0
M
6
F
0
P
0
S
0
T
0
W
0
Y
0
V
Amino Acid Distribution
A: 0 R: 0 N: 0 D: 0 C: 0 E: 0 Q: 0 G: 0 H: 0 I: 0 L: 0 K: 4 M: 0 F: 6 P: 0 S: 0 T: 0 W: 0 Y: 0 V: 0
Chemical Descriptors
10
C78H104N14O11
1413.77
11.00
+4
-2.82
0.120
Free
L
Cationic
Linear
Free
N[C@@H](CC(C)C)C(=O)NCC(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](Cc1ccccc1)C(=O)N[C@@H](CCC(=O)N)C(=O)N[C@@H](CC(=O)O)C(=O)N[C@@H](Cc1ccccc1)C(=O)N[C@@H](CC(=O)N)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](Cc1ccccc1)C(=O)N[C@@H](CC1=NC=NC1)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](Cc1ccccc1)C(=O)N1CCC[C@H]1C(=O)N[C@@H](CCC(=O)N)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](C)C(=O)N[C@@H]([C@@H](C)CC)C(=O)NCC(=O)N[C@@H](C(C)C)C(=O)NCC(=O)N[C@@H](C)C(=O)N1CCC[C@H]1C(=O)O N[C@@H](CCCCN)C(=O)N[C@@H](CC(=O)O)C(=O)N[C@@H](CCCN=C)C(=O)N1CCC[C@H]1C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H](Cc1ccccc1)C(=O)N[C@@H](CCC(=O)N)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC(=O)N)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CO)C(=O)N[C@@H](Cc1ccccc1)C(=O)N[C@@H](CC1=C(C(=NC1)C)CC)C(=O)N[C@@H](CCC(=O)O)C(=O)N[C@@H](CCSC)C(=O)NCC(=O)N[C@@H](C)C(=O)O N[C@@H](CCCCN)C(=O)N[C@@H](Cc1ccccc1)C(=O)N[C@@H](Cc1ccccc1)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](Cc1ccccc1)C(=O)N[C@@H](Cc1ccccc1)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](Cc1ccccc1)C(=O)N[C@@H](Cc1ccccc1)C(=O)N[C@@H](CCCCN)C(=O)O N[C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(=O)O N[C@@H](Cc1ccccc1)C(=O)/N=C/C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCC(=O)N)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)O NCC(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCC(=O)O)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CC1=C(C(=NC1)C)CC)C(=O)N[C@@H](CCCN[C@H](C)CCC)C(=O)N[C@@H](CCC(=O)O)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CC1=C(C(=NC1)C)CC)C(=O)N[C@@H](CC1=C(C(=NC1)C)CC)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CCC(=O)O)C(=O)N[C@@H](CC1=CN=C(C)/C/1=C\C)C(=O)N[C@@H](CO)C(=O)N[C@@H](CCC(=O)N)C(=O)N1CCC[C@H]1C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](C(C)C)C(=O)O
Evidence Records 9 records
Evidence 1 Activity

Activity

Cell-penetrating Peptides

Target

S. Pyogenes Strains

Assay & Model

G. mellonella Larvae
G. mellonella Larvae

Source & Reference

CPPsite3.0
CPPsite3

Other

Source Activity Label Source Definition
Cell-penetrating Peptides
CPPsite3.0 experimentally validated cell-penetrating peptide annotation.
Natural residues Linear L
Synthetic
anti-gyrA antisense PNAs and scrambled PNAs [scPNAs]
In vitro and in vivo
CCSTHHHHTSCCCCCCCCCCCC
Evidence 2 Activity

Activity

Cell-penetrating Peptides

Target

E. Coli Mg1655

Source & Reference

CPPsite3.0
CPPsite3

Other

Natural residues Linear L
Synthetic
PNA scramble
In vitro
CCCSSSSCBTTTBCSCC
Evidence 3 Activity

Activity

Cell-penetrating Peptides

Target

E. Coli Mg1656

Source & Reference

CPPsite3.0
CPPsite3

Other

Natural residues Linear L
Synthetic
anti-MicF 12-1( PNA)
Cultures with 6 microM CPP-PNA 12-1 had equivalent SFGFP fluorescence to those with the fusion reporter alone.
In vitro
CCHHHHTTCC
Evidence 4 Activity

Activity

Cell-penetrating Peptides

Target

Bacterial Cells (Escherichia Coli , Pseudomonas Aeruginosa , Staphylococcus Aureus And Micrococcus Luteus) And Mammalian Cells (ARPE-19, Ncm-460, Muecs, Ct26, And Ftc133 Cells)

Source & Reference

CPPsite3.0
CPPsite3

Other

Natural residues Linear L
Synthetic
Cy7.5-labelled asPNA
The uptake rate of (KFF)3K-asPNA by MDR E. coli was only 14.7%, and that by MRSA was 17.5% and can enter both mammalian and bacterial cells
In vitro
CCCCCCCCCC
Evidence 5 Activity

Activity

Cell-penetrating Peptides

Target

E. Coli Cells

Source & Reference

CPPsite3.0
CPPsite3

Other

Natural residues Linear L Cationic
Synthetic
Anti-acpP PNA
Periplasm and Cytoplasm
Cellular uptake of (KFF)3K-eg1-PNA occurs within 15−30 min
Receptor mediated endocytosis
In vitro
CCTTSSCCCCC
Evidence 6 Activity

Activity

Cell-penetrating Peptides

Target

E. Coli Mg1655

Source & Reference

CPPsite3.0
CPPsite3

Other

Natural residues Linear L
Synthetic
PNA
In vitro
CCCCCCCCSSSCCSSCCSSCCC
Evidence 7 Activity

Activity

0.2±0.03% Hemolysis
Toxicity
64 µM
Hemolytic Cytotoxic
0.2±0.03% Hemolysis | 64 | µM | target cell: Sheep erythrocytes

Target

Sheep erythrocytes

Source & Reference

NA
DBAASP
Triple-targeting Gram-negative selective antimicrobial peptides capable of disrupting the cell membrane and lipid A biosynthesis | RSC Adv | 2016
Evidence 8 Activity

Activity

IC50
Toxicity
256 µM
Cytotoxic
IC50 | 256 | µM | target cell: Murine macrophage cells J774A.1

Target

Murine macrophage cells J774A.1

Source & Reference

NA
DBAASP
Antibacterial Activity of Novel Cationic Peptides against Clinical Isolates of Multi-Drug Resistant Staphylococcus pseudintermedius from Infected Dogs. | PLoS One | 2014
Evidence 9 Activity

Activity

IC50
Toxicity
>256 µM
Cytotoxic
IC50 | >256 | µM | target cell: Human keratinocytes HaCat

Target

Human keratinocytes HaCat

Source & Reference

NA
DBAASP
Antibacterial Activity of Novel Cationic Peptides against Clinical Isolates of Multi-Drug Resistant Staphylococcus pseudintermedius from Infected Dogs. | PLoS One | 2014
Additional Detail Fields 1 fields
CCSTHHHHTSCCCCCCCCCCCC CCCSSSSCBTTTBCSCC CCHHHHTTCC CCCCCCCCCC CCTTSSCCCCC CCCCCCCCSSSCCSSCCSSCCC