TPDB

3D PDB MODEL

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TPDB ID TPDB12171

Activity

Antibacterial Antifungal Toxicity

Activity_category_statistics

Anti-infective peptides Toxicity and safety peptides

Database_Source

AntiBP3 dbAMP DRAMP Peptipedia AFP-GFuse Antifungipept CAFPdb

Sequence_Type

standard

Has_Modification No

Description A 47-aa standard-residue multi-activity (Antibacterial, Antifungal, and Toxicity) peptide sequence curated from AntiBP3, dbAMP, DRAMP, and other sources, with an available 3D structural model.

Sequence

RISFKKGKGSWIKNGLIKGIKGLGKEISLDVIRTGIDIAGCKIKGEC

FASTA

>TPDB12171|Antibacterial;Antifungal;Toxicity|length=47
RISFKKGKGSWIKNGLIKGIKGLGKEISLDVIRTGIDIAGCKIKGEC

Sequence-only estimates for a linear peptide with free termini, not experimentally measured molecular properties. Mass and formula exclude cyclization and disulfide formation. The optical estimates assume paired cysteines, 280 nm, 1 g/L and a 1 cm path. The N-end rule estimate is not a measured plasma half-life.

Formula C225H388N64O61S2

Absent Amino Acids QHMPY

Common Amino Acids GIK

Mass 5030.07

PI 10.43

Basic Residues 11

Acidic Residues 4

Hydrophobic Residues 16

Net Charge +7

Boman Index 1.04

Hydrophobicity -0.074

Aliphatic Index 107.87

N-end Rule Half-life Estimate

Mammalian: 1 hour Yeast: 2 min E.coli: 2 min

Extinction Coefficient Cystines 5625

Absorbance 280nm 1.12

Polar Residues 7

number

1

A

2

R

1

N

2

D

2

C

2

E

0

Q

9

G

0

H

9

I

3

L

9

K

0

M

1

F

0

P

3

S

1

T

1

W

0

Y

1

V

Amino Acid Distribution

A: 1 R: 2 N: 1 D: 2 C: 2 E: 2 Q: 0 G: 9 H: 0 I: 9 L: 3 K: 9 M: 0 F: 1 P: 0 S: 3 T: 1 W: 1 Y: 0 V: 1

Length

47 reviewed residue count

Formula

C225H388N64O61S2 sequence-only estimate (linear, free termini)

Mass

5030.07 sequence-only estimate (linear, free termini)

PI

10.43 sequence-only estimate (linear, free termini)

Net Charge

+7 sequence-only estimate (linear, free termini)

Boman Index

1.04 sequence-only estimate (linear, free termini)

Hydrophobicity

-0.074 sequence-only estimate (linear, free termini)

Evidence 1 Activity

Activity

Activity Endpoint 50% Hemolysis

Detail Source Activity Toxicity

Activity Measurement >480 µM

Toxicity Type Hemolytic Cytotoxic

Toxicity Raw

50% Hemolysis | >480 | µM | target cell: Human erythrocytes

Target

Target Cell Human erythrocytes

Source & Reference

Database Source NA

Source Database DBAASP

Reference

Molecular cloning and characterization of antimicrobial peptides from skin of the broad-folded frog, Hylarana latouchii. | Biochimie | 2012

Evidence 2 Activity

Activity

Activity Endpoint LD50

Detail Source Activity Toxicity

Activity Measurement 480 µM

Toxicity Type Hemolytic

Toxicity Raw

Hemolytic_activity: [Ref.22426384] LD50>480 µM against human erythrocytes

Target

Target Cell erythrocytes

Source & Reference

Database Source NA

Source Database DRAMP

Reference

Biochimie. 2012 Jun 94(6):1317-1326.