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Peptide record

TPDB12103

hBD-3 Antibacterial Anticancer Antifungal Antiparasitic Antiviral Cell-penetrating Peptides Toxicity standard
45 amino acids
Basic Information
3D PDB MODEL
Drag to rotate. Click a residue or atom to inspect it; the selected residue is highlighted in amber.
TPDB12103
hBD-3
Antibacterial Anticancer Antifungal Antiparasitic Antiviral Cell-penetrating Peptides Toxicity
Anti-infective peptides Cancer-related peptides Toxicity and safety peptides Delivery and barrier-penetrating peptides
AntiBP3 dbAMP DRAMP Peptipedia CancerPPD 2.0 dbACP AFP-GFuse Antifungipept CAFPdb dbAMP 3.0 AI4AVP DRAVP 2.0 CPPsite3.0
standard
No
A 45-aa standard natural multi-activity (Antibacterial, Anticancer, Antifungal, and other sources) peptide sequence curated from AntiBP3, dbAMP, DRAMP, and other sources, with an available 3D structural model.
Sequence
GIINTLQKYYCRVRGGRCAVLSCLPKEEQIGKCSTRGRKCCRRKK
Physicochemical Analysis
C216H377N75O59S6
DHMFW
R
5161.20
10.43
13
2
11
+11
12.31
-0.700
67.11
Mammalian: 30 hour Yeast: >20 hour E.coli: >10 hour
3355
65.00
15
Residue Composition
number
1
A
7
R
1
N
0
D
6
C
2
E
2
Q
5
G
0
H
3
I
3
L
6
K
0
M
0
F
1
P
2
S
2
T
0
W
2
Y
2
V
Amino Acid Distribution
A: 1 R: 7 N: 1 D: 0 C: 6 E: 2 Q: 2 G: 5 H: 0 I: 3 L: 3 K: 6 M: 0 F: 0 P: 1 S: 2 T: 2 W: 0 Y: 2 V: 2
Chemical Descriptors
45
C216H377N75O59S6
5161.20
10.43
+11
12.31
-0.700
Free
L
Linear
Free
NCC(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H](CC(=O)N)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCC(=O)N)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](Cc1ccccc1)C(=O)N[C@@H](Cc1ccccc1)C(=O)N[C@@H](CS)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](CCCN=C)C(=O)NCC(=O)NCC(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CS)C(=O)N[C@@H](C)C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CO)C(=O)N[C@@H](CS)C(=O)N[C@@H](CC(C)C)C(=O)N1CCC[C@H]1C(=O)N[C@@H](/C=C/CCN)C(=O)N[C@@H](CCC(=O)O)C(=O)N[C@@H](CCC(=O)O)C(=O)N[C@@H](CCC(=O)N)C(=O)N[C@@H]([C@@H](C)CC)C(=O)NCC(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CS)C(=O)N[C@@H](CO)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CCCN=C)C(=O)NCC(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CS)C(=O)N[C@@H](CS)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(=O)O
Evidence Records 25 records
Evidence 1 Activity

Activity

MIC
Antibacterial
9.4 µg/mL

Target

Escherichia coli DSM1103

Source & Reference

dbAMP DRAMP

Other

Source Activity Label Source Definition
Antibacterial
dbAMP classified as Antibacterial under the Anti-bacterial function class. DRAMP antibacterial activity supported by MIC records against bacterial target organisms.
An inducible human AMP. Active against E. coli DSM1103 (MIC 9.4 ug/ml), K. pneumoniae DSM681 (MIC 25 ug/ml), P. aeruginosa DSM1128 (MIC 18.75 ug/ml), S. aureus
Evidence 2 Activity

Activity

MIC
Antibacterial
25 µg/mL

Target

Klebsiella pneumoniae DSM681

Source & Reference

dbAMP DRAMP

Other

An inducible human AMP. Active against E. coli DSM1103 (MIC 9.4 ug/ml), K. pneumoniae DSM681 (MIC 25 ug/ml), P. aeruginosa DSM1128 (MIC 18.75 ug/ml), S. aureus ATCC25923 or MRSA (MIC 4.7 ug/ml), an
Evidence 3 Activity

Activity

MIC
Antibacterial
18.75 µg/mL

Target

Pseudomonas aeruginosa DSM1128

Source & Reference

dbAMP DRAMP

Other

AMP. Active against E. coli DSM1103 (MIC 9.4 ug/ml), K. pneumoniae DSM681 (MIC 25 ug/ml), P. aeruginosa DSM1128 (MIC 18.75 ug/ml), S. aureus ATCC25923 or MRSA (MIC 4.7 ug/ml), and S. pneumoniae DSM11865 (MIC 4.7 ug/ml)
Evidence 4 Activity

Activity

MIC
Antibacterial
4.7 µg/mL

Target

Staphylococcus aureus ATCC25923 or MRSA

Source & Reference

dbAMP DRAMP

Other

9.4 ug/ml), K. pneumoniae DSM681 (MIC 25 ug/ml), P. aeruginosa DSM1128 (MIC 18.75 ug/ml), S. aureus ATCC25923 or MRSA (MIC 4.7 ug/ml), and S. pneumoniae DSM11865 (MIC 4.7 ug/ml) (ref see AP1315). LD90 (lethal dose killing >
Evidence 5 Activity

Activity

MIC
Antibacterial
4.7 µg/mL

Target

S. pneumoniae DSM11865

Source & Reference

dbAMP DRAMP

Other

P. aeruginosa DSM1128 (MIC 18.75 ug/ml), S. aureus ATCC25923 or MRSA (MIC 4.7 ug/ml), and S. pneumoniae DSM11865 (MIC 4.7 ug/ml) (ref see AP1315). LD90 (lethal dose killing > 90%): active against E.coli WBB01 or ATCC 1
Evidence 6 Activity

Activity

MIC
Antifungal
9.4 µg/mL

Target

Escherichia coli DSM1103

Source & Reference

dbAMP DRAMP CAFPdb Antifungipept

Other

Source Activity Label Source Definition
Antifungal
dbAMP classified under the Anti-fungal function class. DRAMP antifungal activity supported by activity records against fungal target organisms.
An inducible human AMP. Active against E. coli DSM1103 (MIC 9.4 ug/ml), K. pneumoniae DSM681 (MIC 25 ug/ml), P. aeruginosa DSM1128 (MIC 18.75 ug/ml), S. aureus
Evidence 7 Activity

Activity

MIC
Antifungal
25 µg/mL

Target

Klebsiella pneumoniae DSM681

Source & Reference

dbAMP DRAMP CAFPdb Antifungipept

Other

An inducible human AMP. Active against E. coli DSM1103 (MIC 9.4 ug/ml), K. pneumoniae DSM681 (MIC 25 ug/ml), P. aeruginosa DSM1128 (MIC 18.75 ug/ml), S. aureus ATCC25923 or MRSA (MIC 4.7 ug/ml), an
Evidence 8 Activity

Activity

MIC
Antifungal
18.75 µg/mL

Target

Pseudomonas aeruginosa DSM1128

Source & Reference

dbAMP DRAMP CAFPdb Antifungipept

Other

AMP. Active against E. coli DSM1103 (MIC 9.4 ug/ml), K. pneumoniae DSM681 (MIC 25 ug/ml), P. aeruginosa DSM1128 (MIC 18.75 ug/ml), S. aureus ATCC25923 or MRSA (MIC 4.7 ug/ml), and S. pneumoniae DSM11865 (MIC 4.7 ug/ml)
Evidence 9 Activity

Activity

MIC
Antifungal
4.7 µg/mL

Target

Staphylococcus aureus ATCC25923 or MRSA

Source & Reference

dbAMP DRAMP CAFPdb Antifungipept

Other

9.4 ug/ml), K. pneumoniae DSM681 (MIC 25 ug/ml), P. aeruginosa DSM1128 (MIC 18.75 ug/ml), S. aureus ATCC25923 or MRSA (MIC 4.7 ug/ml), and S. pneumoniae DSM11865 (MIC 4.7 ug/ml) (ref see AP1315). LD90 (lethal dose killing >
Evidence 10 Activity

Activity

MIC
Antifungal
4.7 µg/mL

Target

S. pneumoniae DSM11865

Source & Reference

dbAMP DRAMP CAFPdb Antifungipept

Other

P. aeruginosa DSM1128 (MIC 18.75 ug/ml), S. aureus ATCC25923 or MRSA (MIC 4.7 ug/ml), and S. pneumoniae DSM11865 (MIC 4.7 ug/ml) (ref see AP1315). LD90 (lethal dose killing > 90%): active against E.coli WBB01 or ATCC 1
Evidence 11 Activity

Activity

MIC
Antiparasitic
9.4 µg/mL

Target

Escherichia coli DSM1103

Source & Reference

dbAMP 3.0 DRAMP

Other

Source Activity Label Source Definition
Antiparasitic
DRAMP antiparasitic activity supported by activity records against parasitic targets. dbAMP 3.0 classified under the Anti-parasitic function class.
An inducible human AMP. Active against E. coli DSM1103 (MIC 9.4 ug/ml), K. pneumoniae DSM681 (MIC 25 ug/ml), P. aeruginosa DSM1128 (MIC 18.75 ug/ml), S. aureus
Evidence 12 Activity

Activity

MIC
Antiparasitic
25 µg/mL

Target

Klebsiella pneumoniae DSM681

Source & Reference

dbAMP 3.0 DRAMP

Other

An inducible human AMP. Active against E. coli DSM1103 (MIC 9.4 ug/ml), K. pneumoniae DSM681 (MIC 25 ug/ml), P. aeruginosa DSM1128 (MIC 18.75 ug/ml), S. aureus ATCC25923 or MRSA (MIC 4.7 ug/ml), an
Evidence 13 Activity

Activity

MIC
Antiparasitic
18.75 µg/mL

Target

Pseudomonas aeruginosa DSM1128

Source & Reference

dbAMP 3.0 DRAMP

Other

AMP. Active against E. coli DSM1103 (MIC 9.4 ug/ml), K. pneumoniae DSM681 (MIC 25 ug/ml), P. aeruginosa DSM1128 (MIC 18.75 ug/ml), S. aureus ATCC25923 or MRSA (MIC 4.7 ug/ml), and S. pneumoniae DSM11865 (MIC 4.7 ug/ml)
Evidence 14 Activity

Activity

MIC
Antiparasitic
4.7 µg/mL

Target

Staphylococcus aureus ATCC25923 or MRSA

Source & Reference

dbAMP 3.0 DRAMP

Other

9.4 ug/ml), K. pneumoniae DSM681 (MIC 25 ug/ml), P. aeruginosa DSM1128 (MIC 18.75 ug/ml), S. aureus ATCC25923 or MRSA (MIC 4.7 ug/ml), and S. pneumoniae DSM11865 (MIC 4.7 ug/ml) (ref see AP1315). LD90 (lethal dose killing >
Evidence 15 Activity

Activity

MIC
Antiparasitic
4.7 µg/mL

Target

S. pneumoniae DSM11865

Source & Reference

dbAMP 3.0 DRAMP

Other

P. aeruginosa DSM1128 (MIC 18.75 ug/ml), S. aureus ATCC25923 or MRSA (MIC 4.7 ug/ml), and S. pneumoniae DSM11865 (MIC 4.7 ug/ml) (ref see AP1315). LD90 (lethal dose killing > 90%): active against E.coli WBB01 or ATCC 1
Evidence 16 Activity

Activity

MIC
Antiviral
9.4 µg/mL

Target

Escherichia coli DSM1103

Source & Reference

dbAMP 3.0 DRAMP DRAVP 2.0

Other

Source Activity Label Source Definition
Antiviral
DRAMP antiviral activity supported by activity records against viral targets. dbAMP 3.0 classified under the Anti-viral function class.
An inducible human AMP. Active against E. coli DSM1103 (MIC 9.4 ug/ml), K. pneumoniae DSM681 (MIC 25 ug/ml), P. aeruginosa DSM1128 (MIC 18.75 ug/ml), S. aureus
Evidence 17 Activity

Activity

MIC
Antiviral
25 µg/mL

Target

Klebsiella pneumoniae DSM681

Source & Reference

dbAMP 3.0 DRAMP DRAVP 2.0

Other

An inducible human AMP. Active against E. coli DSM1103 (MIC 9.4 ug/ml), K. pneumoniae DSM681 (MIC 25 ug/ml), P. aeruginosa DSM1128 (MIC 18.75 ug/ml), S. aureus ATCC25923 or MRSA (MIC 4.7 ug/ml), an
Evidence 18 Activity

Activity

MIC
Antiviral
18.75 µg/mL

Target

Pseudomonas aeruginosa DSM1128

Source & Reference

dbAMP 3.0 DRAMP DRAVP 2.0

Other

AMP. Active against E. coli DSM1103 (MIC 9.4 ug/ml), K. pneumoniae DSM681 (MIC 25 ug/ml), P. aeruginosa DSM1128 (MIC 18.75 ug/ml), S. aureus ATCC25923 or MRSA (MIC 4.7 ug/ml), and S. pneumoniae DSM11865 (MIC 4.7 ug/ml)
Evidence 19 Activity

Activity

MIC
Antiviral
4.7 µg/mL

Target

Staphylococcus aureus ATCC25923 or MRSA

Source & Reference

dbAMP 3.0 DRAMP DRAVP 2.0

Other

9.4 ug/ml), K. pneumoniae DSM681 (MIC 25 ug/ml), P. aeruginosa DSM1128 (MIC 18.75 ug/ml), S. aureus ATCC25923 or MRSA (MIC 4.7 ug/ml), and S. pneumoniae DSM11865 (MIC 4.7 ug/ml) (ref see AP1315). LD90 (lethal dose killing >
Evidence 20 Activity

Activity

MIC
Antiviral
4.7 µg/mL

Target

S. pneumoniae DSM11865

Source & Reference

dbAMP 3.0 DRAMP DRAVP 2.0

Other

P. aeruginosa DSM1128 (MIC 18.75 ug/ml), S. aureus ATCC25923 or MRSA (MIC 4.7 ug/ml), and S. pneumoniae DSM11865 (MIC 4.7 ug/ml) (ref see AP1315). LD90 (lethal dose killing > 90%): active against E.coli WBB01 or ATCC 1
Evidence 21 Activity

Activity

Cell-penetrating Peptides

Target

Polarized Tonsil Epithelial Cells , Ex-Vivo Infant Tonsil Tissues

Source & Reference

CPPsite3.0
CPPsite3

Other

Source Activity Label Source Definition
Cell-penetrating Peptides
CPPsite3.0 experimentally validated cell-penetrating peptide annotation.
Natural residues Linear L
Synthetic
Cytoplasm
Endocytosis
In vitro and ex vivo
CCHHHHHHHHHTSSSCCCCSSCCCSCSSCSSSSSCCBTTBSSCCC
Evidence 22 Activity

Activity

Toxicity
Hemolytic Cytotoxic
- | NA | target cell: Human erythrocytes

Target

Human erythrocytes

Source & Reference

NA
DBAASP
Isolation and characterization of human beta -defensin-3, a novel human inducible peptide antibiotic. | J Biol Chem | 2001
Evidence 23 Activity

Activity

0% Hemolysis
Toxicity
40 µg/mL
Hemolytic Cytotoxic
0% Hemolysis | 40 | µg/mL | target cell: Human erythrocytes

Target

Human erythrocytes

Source & Reference

NA
DBAASP
A Chimeric Cationic Peptide Composed of Human β-Defensin 3 and Human β-Defensin 4 Exhibits Improved Antibacterial Activity and Salt Resistance. | Front Microbiol | 2021
Evidence 24 Activity

Activity

20% Killing
Toxicity
40 µg/mL
Cytotoxic
20% Killing | 40 | µg/mL | target cell: Vero cells

Target

Vero cells

Source & Reference

NA
DBAASP
A Chimeric Cationic Peptide Composed of Human β-Defensin 3 and Human β-Defensin 4 Exhibits Improved Antibacterial Activity and Salt Resistance. | Front Microbiol | 2021
Evidence 25 Activity

Activity

% Hemolysis
Toxicity
9 %
Hemolytic
Hemolytic_activity: [Ref.11085990] It has 9% hemolysis at 250 µg/ml, 15% hemolysis at 500 µg/ml against human erythrocytes.

Target

erythrocytes

Source & Reference

NA
DRAMP
Biopolymers. 2005 80(1):34-49.##J Biol Chem. 2001 Feb 23 276(8):5707-5713.
Additional Detail Fields 1 fields
CCHHHHHHHHHTSSSCCCCSSCCCSCSSCSSSSSCCBTTBSSCCC