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Peptide record

TPDB11984

Crotamine Antibacterial Anticancer Antifungal Antiparasitic Antiviral Cell-penetrating Peptides Hemolysis Toxicity standard
42 amino acids
Basic Information
3D PDB MODEL
Drag to rotate. Click a residue or atom to inspect it; the selected residue is highlighted in amber.
TPDB11984
Crotamine
Antibacterial Anticancer Antifungal Antiparasitic Antiviral Cell-penetrating Peptides Hemolysis Toxicity
Anti-infective peptides Cancer-related peptides Toxicity and safety peptides Delivery and barrier-penetrating peptides
AntiBP3 dbAMP DRAMP Peptipedia ACP740 _ACP240 AntiCP 2.0 SATPdb dbACP iACP-DRLF AFP-GFuse Antifungipept CAFPdb dbAMP 3.0 i2APP AVPpred-BWR CPPsite3.0 MLCPP 2.0 APD_complete APD_hemolytic HemoPI Hemolytic2 PD_Hemolysis
standard
No
A 42-aa standard natural multi-activity (Antibacterial, Anticancer, Antifungal, and other sources) peptide sequence curated from AntiBP3, dbAMP, DRAMP, and other sources, with an available 3D structural model.
Sequence
YKQCHKKGGHCFPKEKICLPPSSDFGKMDCRWRWKCCKKGSG
Physicochemical Analysis
C214H332N64O54S7
ANTV
K
4889.81
9.75
13
3
8
+8
9.86
-1.095
18.57
Mammalian: 2.8 hour Yeast: 10 min E.coli: 2 min
12865
263.10
11
Residue Composition
number
0
A
2
R
0
N
2
D
6
C
1
E
1
Q
5
G
2
H
1
I
1
L
9
K
1
M
2
F
3
P
3
S
0
T
2
W
1
Y
0
V
Amino Acid Distribution
A: 0 R: 2 N: 0 D: 2 C: 6 E: 1 Q: 1 G: 5 H: 2 I: 1 L: 1 K: 9 M: 1 F: 2 P: 3 S: 3 T: 0 W: 2 Y: 1 V: 0
Chemical Descriptors
42
C214H332N64O54S7
4889.81
9.75
+8
9.86
-1.095
Free
L
Amphipathic Cationic
Linear
Free
N[C@@H](Cc1ccccc1)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCC(=O)N)C(=O)N[C@@H](CS)C(=O)N[C@@H](Cc1[nH]cnc1)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(=O)NCC(=O)NCC(=O)N[C@@H](CC1=NC=NC1)C(=O)N[C@@H](CS)C(=O)N[C@@H](Cc1ccccc1)C(=O)N1CCC[C@H]1C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCC(=O)O)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H](CS)C(=O)N[C@@H](CC(C)C)C(=O)N1CCC[C@H]1C(=O)N1CCC[C@H]1C(=O)N[C@@H](CO)C(=O)N[C@@H](CO)C(=O)N[C@@H](CC(=O)O)C(=O)N[C@@H](Cc1ccccc1)C(=O)NCC(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCSC)C(=O)N[C@@H](CC(=O)O)C(=O)N[C@@H](CS)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CC1=C(C(=NC1)C)CC)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CC1=CN=C(C)/C/1=C\C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CS)C(=O)N[C@@H](CS)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(=O)NCC(=O)N[C@@H](CO)C(=O)NCC(=O)O N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H](CCCN=C)C(=O)N1CCC[C@H]1C(=O)N[C@@H](CCCN=C)C(=O)N1CCC[C@H]1C(=O)N1CCC[C@H]1C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CC(C)C)C(=O)N1CCC[C@H]1C(=O)N[C@@H](CCCN=C)C(=O)N1CCC[C@H]1C(=O)N[C@@H](CCCN=C)C(=O)N1CCC[C@H]1C(=O)N[C@@H](CCCN=C)C(=O)N1CCC[C@H]1C(=O)N[C@@H](CC(C)C)C(=O)N1CCC[C@H]1C(=O)N[C@@H](Cc1ccccc1)C(=O)N1CCC[C@H]1C(=O)N[C@@H](CCCN=C)C(=O)N1CCC[C@H]1C(=O)NCC(=O)O N[C@@H](CCCCN)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCC(=O)O)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCN=C)C(=O)O N[C@@H]([C@@H](C)CC)C(=O)N1CCC[C@H]1C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](C(C)C)C(=O)N1CCC[C@H]1C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CS)C(=O)O
Evidence Records 17 records
Evidence 1 Activity

Activity

Antibacterial

Source & Reference

dbAMP DRAMP

Other

Source Activity Label Source Definition
Antibacterial
dbAMP classified as Antibacterial under the Anti-bacterial function class.
It's now found to have multiple functions, including cell-penetrating (targeting lysosomes) and antimicrobial activities (see ref). Active against P. falciparum. Crotamine preferentially targets eukaryotic cells over prokaryotic Kv channels.
Evidence 2 Activity

Activity

Antifungal

Source & Reference

dbAMP DRAMP CAFPdb Antifungipept

Other

Source Activity Label Source Definition
Antifungal
dbAMP classified under the Anti-fungal function class.
It's now found to have multiple functions, including cell-penetrating (targeting lysosomes) and antimicrobial activities (see ref). Active against P. falciparum. Crotamine preferentially targets eukaryotic cells over prokaryotic Kv channels.
Evidence 3 Activity

Activity

Antiparasitic

Source & Reference

dbAMP 3.0 DRAMP

Other

Source Activity Label Source Definition
Antiparasitic
dbAMP 3.0 classified under the Anti-parasitic function class.
It's now found to have multiple functions, including cell-penetrating (targeting lysosomes) and antimicrobial activities (see ref). Active against P. falciparum. Crotamine preferentially targets eukaryotic cells over prokaryotic Kv channels.
Evidence 4 Activity

Activity

Antiviral

Source & Reference

dbAMP 3.0 DRAMP

Other

Source Activity Label Source Definition
Antiviral
dbAMP 3.0 classified under the Anti-viral function class.
It's now found to have multiple functions, including cell-penetrating (targeting lysosomes) and antimicrobial activities (see ref). Active against P. falciparum. Crotamine preferentially targets eukaryotic cells over prokaryotic Kv channels.
Evidence 5 Activity

Activity

Cell-penetrating Peptides

Target

Mice melanoma B16F10 and HEK293(3), CHO-K1

Assay & Model

B16F190 tumor bearing NMRI nude mice
B16F190 tumor bearing NMRI nude mice

Source & Reference

CPPsite3.0
CPPsite3

Other

Source Activity Label Source Definition
Cell-penetrating Peptides
CPPsite3.0 experimentally validated cell-penetrating peptide annotation.
Natural residues Linear L
Protein derived
Fluorophore (Alexa 700)
Cytoplasm
In vitro and in vivo
CCSSCCSSCCSCCSSSSSSCSCCCSCCCCCHHHHCCCSCC
Evidence 6 Activity

Activity

Cell-penetrating Peptides

Target

B16-F10, HCT116, 3T3 cells

Assay & Model

C57BL6 or nude mice
C57BL6 or nude mice

Source & Reference

CPPsite3.0
CPPsite3

Other

Natural residues Linear L
Protein derived
Nucleic acid
Cytoplasm
In vitro and in vivo
CCSSCCSSCCSCCSSSSSSCSCCCSCCCCCHHHHCCCSCC
Evidence 7 Activity

Activity

Cell-penetrating Peptides

Target

Ai9 Tdtomato Npcs

Assay & Model

Ai9 or wild-type mice
Ai9 or wild-type mice

Source & Reference

CPPsite3.0
CPPsite3

Other

Natural residues Linear L
SpyCas9 RNPs
In vitro and in vivo
CCCCCCSCSSSCCCSSCSSCCSCC
Evidence 8 Activity

Activity

Cell-penetrating Peptides

Target

P. Falciparum Infected Erythrocytes

Source & Reference

CPPsite3.0
CPPsite3
PCT/BR06/000052 US-2008-0181849-A1 1866332

Other

Natural residues Linear L Amphipathic
Protein derived
Cy3-labelled
Nucleus
After 1 h of incubation with 10 μM of Cy3-crotamine, the toxin was visualized within the parasites inside the infected red blood cells (iRBCs) and not in uninfected RBCs.
In vitro
CCCCSTTTSSCCC
Evidence 9 Activity

Activity

Cell-penetrating Peptides

Assay & Model

Fertilized (IVF) bovine embryos.
Fertilized (IVF) bovine embryos.

Source & Reference

CPPsite3.0
CPPsite3

Other

Natural residues Linear L Cationic
Protein derived
Rhodamine B-labelled at N-terminus
Crotamine translocation through ZP of IVF bovine zygotes after 6 h exposure and partial overlapped with DAPI nuclear DNA staining.
In vivo
CTTTCCCTTTGGGTCC
Evidence 10 Activity

Activity

Cell-penetrating Peptides

Assay & Model

IVF bovine embryos and parthenogenetic (PA) embryos
IVF bovine embryos and parthenogenetic (PA) embryos

Source & Reference

CPPsite3.0
CPPsite3

Other

Natural residues Linear L Cationic
Protein derived
Rhodamine B-labelled at N-terminus and pCX-EGFP plasmid.
Control embryos showed no red fluorescence signal, while blue fluorescence signal was present for embryos of both groups.
In vivo
CCSSCCSSCCSCCSSSSSSCSCCCSCCCCCHHHHCCCSCC
Evidence 11 Activity

Activity

Toxicity
Hemolytic Cytotoxic
- | NA | target cell: Mouse erythrocytes

Target

Mouse erythrocytes

Source & Reference

NA
DBAASP
In vitro antibacterial and hemolytic activities of crotamine, a small basic myotoxin from rattlesnake Crotalus durissus. | J Antibiot (Tokyo) | 2011
Evidence 12 Activity

Activity

Toxicity
Hemolytic Cytotoxic
- | NA | target cell: Human erythrocytes

Target

Human erythrocytes

Source & Reference

NA
DBAASP
Unraveling the antifungal activity of a South American rattlesnake toxin crotamine. | Biochimie | 2013
Evidence 13 Activity

Activity

50% Cell death
Toxicity
>50 µM
Cytotoxic
50% Cell death | >50 | µM | target cell: Human embryonic kidney HEK293 cells

Target

Human embryonic kidney HEK293 cells

Source & Reference

NA
DBAASP
Unraveling the antifungal activity of a South American rattlesnake toxin crotamine. | Biochimie | 2013
Evidence 14 Activity

Activity

3% Cell death
Toxicity
50 µg/mL
Cytotoxic
3% Cell death | 50 | µg/mL | target cell: Bone marrow-derived macrophages (BMDMs)

Target

Bone marrow-derived macrophages (BMDMs)

Source & Reference

NA
DBAASP
Effect of Isolated Proteins from Crotalus Durissus Terrificus Venom on Leishmania (Leishmania) Amazonensis-Infected Macrophages. | Protein Pept Lett | 2020
Evidence 15 Activity

Activity

35% Hemolysis
Toxicity
1024 µg/mL
Hemolytic Cytotoxic
35% Hemolysis | 1024 | µg/mL | target cell: Mouse erythrocytes

Target

Mouse erythrocytes

Source & Reference

NA
DBAASP
In vitro antibacterial and hemolytic activities of crotamine, a small basic myotoxin from rattlesnake Crotalus durissus. | J Antibiot (Tokyo) | 2011
Evidence 16 Activity

Activity

Toxicity
Hemolytic
Hemolytic_activity: [Ref.210622]Non-hemolytic activity

Target

erythrocytes

Source & Reference

NA
DRAMP
Proc Natl Acad Sci U S A. 2009 Sep 1 106(35):14972-14977.##Toxicon. 2005 Dec 1 46(7):759-767.##Hoppe Seylers Z Physiol Chem. 1975 Feb 356(2):213-215.##Toxicon. 1998 Dec 36(12):1927-1937.##Mol Pharm. 2012 Feb 6
Evidence 17 Hemolysis

Source & Reference

APD_complete APD_hemolytic HemoPI PD_Hemolysis
APD_complete APD_hemolytic PD_Hemolysis

Other

Source Activity Label Source Definition
Hemolysis
APD_complete APD-derived hemolytic peptide annotation. APD_hemolytic APD hemolytic peptide annotation.
Additional Detail Fields 1 fields
CCSSCCSSCCSCCSSSSSSCSCCCSCCCCCHHHHCCCSCC CCCCCCSCSSSCCCSSCSSCCSCC CCCCSTTTSSCCC CTTTCCCTTTGGGTCC