TPDB

3D PDB MODEL

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TPDB ID TPDB11875

Peptide_Name

MP1102

Activity

Antibacterial Cell-penetrating Peptides Toxicity

Activity_category_statistics

Anti-infective peptides Toxicity and safety peptides Delivery and barrier-penetrating peptides

Database_Source

AntiBP3 Peptipedia CPPsite3.0

Sequence_Type

standard

Has_Modification No

Description A 40-aa standard natural multi-activity (Antibacterial, Cell-penetrating Peptides, and Toxicity) peptide sequence curated from AntiBP3, Peptipedia, and CPPsite3.0, with an available 3D structural model.

Sequence

GFGCNGPWQEDDVKCHNHCKSIKGYKGGYCAKGGFVCKCY

FASTA

>TPDB11875|Antibacterial;Cell-penetrating Peptides;Toxicity|length=40
GFGCNGPWQEDDVKCHNHCKSIKGYKGGYCAKGGFVCKCY

Formula C191H280N54O54S6

Absent Amino Acids RLMT

Common Amino Acids G

Mass 4389.01

PI 8.30

Basic Residues 8

Acidic Residues 3

Hydrophobic Residues 10

Net Charge +3

Boman Index 5.71

Hydrophobicity -0.647

Aliphatic Index 26.75

Half Life

Mammalian: 30 hour Yeast: >20 hour E.coli: >10 hour

Extinction Coefficient Cystines 10345

Absorbance 280nm 235.70

Polar Residues 13

number

1

A

0

R

2

N

2

D

6

C

1

E

1

Q

8

G

2

H

1

I

0

L

6

K

0

M

2

F

1

P

1

S

0

T

1

W

3

Y

2

V

Amino Acid Distribution

A: 1 R: 0 N: 2 D: 2 C: 6 E: 1 Q: 1 G: 8 H: 2 I: 1 L: 0 K: 6 M: 0 F: 2 P: 1 S: 1 T: 0 W: 1 Y: 3 V: 2

Length

40

Formula

C191H280N54O54S6

Mass

4389.01

PI

8.30

Net Charge

+3

Boman Index

5.71

Hydrophobicity

-0.647

C_terminal_Modification

Free

Chirality

L

Linearity

Linear

N_terminal_Modification

Free

SMILES

N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)NCC(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)NCC(=O)O

Evidence 1 Activity

Activity

Activity Endpoint MIC

Detail Source Activity Antibacterial

Activity Measurement 0.004-0.23 µM

Target

Target Organism Staphylococcus aureus ATCC25923 or ATCC29213 or ATCC6538 or ATCC43300 and 20 MRSA clinical strains

Source & Reference

Database Source NA

Other

Evidence Text

idermidis ATCC 26069 (14.33 mm), and S. aureus ATCC 25923 (34.44 mm) but not E.coli. MIC: S. aureus ATCC25923 or ATCC29213 or ATCC6538 or ATCC43300 and 20 MRSA clinical strains (MIC 0.004-0.23 uM). 14-fold more active against MRSA ATCC43300 than NZ2114.

Evidence 2 Activity

Activity

Detail Source Activity Cell-penetrating Peptides

Target

Cell Line RAW 264.7 Macrophages

Assay & Model

In Vitro Model Six-week-old specifc pathogen-free female ICR mice

In Vivo Model Six-week-old specifc pathogen-free female ICR mice

Source & Reference

Database Source CPPsite3.0

Source CPPsite3

Other

Source Activity Label	Source Definition
Cell-penetrating Peptides	CPPsite3.0 experimentally validated cell-penetrating peptide annotation.

CPP Category Natural residues Linear L

Source of Peptide Synthetic

Cargo Type FITC-labeled

Subcellular Localization Cytoplasm

Uptake Efficiency

The percentages of PI-staining in cells were 0.51% at 250 μg/ml, respectively, indicating that MP1102 had poor cell penetrability.

Uptake Mechanism Macropinocytosis and clathrin mediated endocytosis

In Vitro or In Vivo In vitro and in vivo

DSSP Secondary Structure CCSCCC

Evidence 3 Activity

Activity

Activity Endpoint 0% Hemolysis

Detail Source Activity Toxicity

Activity Measurement 128 µg/mL

Toxicity Type Hemolytic Cytotoxic

Toxicity Raw

0% Hemolysis | 128 | µg/mL | target cell: Human erythrocytes

Target

Target Cell Human erythrocytes

Source & Reference

Database Source NA

Source Database DBAASP

Reference

In vitro and in vivo characterization of a new recombinant antimicrobial peptide, MP1102, against methicillin-resistant Staphylococcus aureus. | Appl Microbiol Biotechnol | 2015

DSSP_Secondary_Structure

CCSCCC