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Peptide record

TPDB11385

Antibacterial Anticancer Antifungal Toxicity standard
32 amino acids
Basic Information
3D PDB MODEL
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TPDB11385
Antibacterial Anticancer Antifungal Toxicity
Anti-infective peptides Cancer-related peptides Toxicity and safety peptides
AntiBP3 dbAMP DRAMP Peptipedia Antifungipept CAFPdb
standard
No
A 32-aa standard natural multi-activity (Antibacterial, Anticancer, Antifungal, and other sources) peptide sequence curated from AntiBP3, dbAMP, DRAMP, and other sources, with an available 3D structural model.
Sequence
SFLTTVKKLVTNLAALAGTVIDTIKCKVTGGC
Physicochemical Analysis
C145H253N37O43S2
REQHMPWY
T
3266.95
9.72
4
1
14
+3
1.91
0.794
118.75
Mammalian: 1.9 hour Yeast: >20 hour E.coli: >10 hour
125
3.83
10
Residue Composition
number
3
A
0
R
1
N
1
D
2
C
0
E
0
Q
3
G
0
H
2
I
4
L
4
K
0
M
1
F
0
P
1
S
6
T
0
W
0
Y
4
V
Amino Acid Distribution
A: 3 R: 0 N: 1 D: 1 C: 2 E: 0 Q: 0 G: 3 H: 0 I: 2 L: 4 K: 4 M: 0 F: 1 P: 0 S: 1 T: 6 W: 0 Y: 0 V: 4
Chemical Descriptors
32
C145H253N37O43S2
3266.95
9.72
+3
1.91
0.794
Evidence Records 2 records
Evidence 1 Activity

Activity

50% Hemolysis
Toxicity
63.90 µM
Hemolytic Cytotoxic
50% Hemolysis | 63.90 | µM | target cell: Horse erythrocytes

Target

Horse erythrocytes

Source & Reference

NA
DBAASP
Bioevaluation of Ranatuerin-2Pb from the Frog Skin Secretion of Rana pipiens and its Truncated Analogues. | Biomolecules | 2019
Evidence 2 Activity

Activity

% Hemolysis
Toxicity
7 %
Hemolytic Cytotoxic
Hemolytic_activity: [Ref.31242693] 7% hemolysis at 16 µM, 23% hemolysis at 32 µM, 50% hemolysis at 64 µM, 76% hemolysis at 128 µM, 90% hemolysis at 256 µM, 100% hemolysis at 512 µM on horse erythrocytes. | Cytotoxicity: [Ref.31242693] RPa only possessed inhibitory effect on the proliferation of H157 with the IC value of 5.841 µM, while showed no inhibitory effect on the proliferation of MCF-7, U251MG, PC-3 and MDA-MB-435s. DETAILED DATA: ①The cell viabillity of NCI-H157 induced by RPa is 97.5%, 94.4%, 92.5%, 71.2%, 40.6% and 4.4% at peptide concentrations of 0.001, 0.01, 0.1, 1, 10, 100 µM, while the cell viability induced by Melittin is 100%, 95%, 89.4%, 41.2%, 16.2% and 16.2% at 0.001, 0.01, 0.1, 1, 10, 100 µM. ②The cell viability of MCF-7 induced by RPa is 100.6%, 93.1%, 97.5%, 97.5%, 93.7% and 78.1% at peptide concentrations of 0.001, 0.01, 0.1, 1, 10, 100 µM , while the cell viability induced by Melittin is 100%, 98.1%, 92.5%, 7.5%, 8.7% and 8.7% at 0.001, 0.01, 0.1, 1, 10, 100 µM. ③The cell viability of U251-MG induced by RPa is 100%, 105.6%, 108.1%, 102.5%, 59.4% and 17.5% at peptide concentrations of 0.001, 0.01, 0.1, 1, 10, 100 µM, while the cell viability induced by Melittin is 99.4%, 99.4%, 75.6%, 18.7%, 11.9% and 18.1% at 0.001, 0.01, 0.1, 1, 10, 100 µM.④The cell viability of PC-3 induced by RPb is 108.7%, 104.4%, 92.5%, 92.5%, 82.5% and 24.4% at peptide concentrations of 0.001, 0.01, 0.1, 1, 10, 100 µM, while the cell viability induced by Melittin is 110%, 113.1%, 109.4%, 33.1%, 16.9% and 16.2% at 0.001, 0.01, 0.1, 1, 10, 100 µM. ⑤The cell viability of MDA-MB-435S induced by RPa is 97.5%, 95%, 97.5%, 73.1%, 68.8% and 36.9%, while the cell viability induced by Melittin is 100%, 93.1%, 82.5%, 18.8%, 18.8% and 18.1%.

Target

erythrocytes

Source & Reference

NA
DRAMP
Biomolecules. 2019 Jun 25 9(6):249. doi: 10.3390/biom9060249.