TPDB

3D PDB MODEL

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TPDB ID TPDB11274

Activity

Antibacterial Anticancer Hemolysis Toxicity

Activity_category_statistics

Anti-infective peptides Cancer-related peptides Toxicity and safety peptides

Database_Source

AntiBP3 Peptipedia HemoPI Hemolytic2 PD_Hemolysis ToxinPred 3.0

Sequence_Type

standard

Has_Modification No

Description A 31-aa standard natural multi-activity (Antibacterial, Anticancer, Hemolysis, and other sources) peptide sequence curated from AntiBP3, Peptipedia, HemoPI, and other sources, with an available 3D structural model.

Sequence

GIPCAESCVWIPCTITALMGCSCKNNVCYNN

FASTA

>TPDB11274|Antibacterial;Anticancer;Hemolysis;Toxicity|length=31
GIPCAESCVWIPCTITALMGCSCKNNVCYNN

Formula C138H219N37O43S7

Absent Amino Acids RDQHF

Common Amino Acids C

Mass 3308.90

PI 5.90

Basic Residues 1

Acidic Residues 1

Hydrophobic Residues 11

Net Charge -0

Boman Index 0.00

Hydrophobicity 0.503

Aliphatic Index 75.48

Half Life

Mammalian: 30 hour Yeast: >20 hour E.coli: >10 hour

Extinction Coefficient Cystines 7365

Absorbance 280nm 222.58

Polar Residues 15

number

2

A

0

R

4

N

0

D

6

C

1

E

0

Q

2

G

0

H

3

I

1

L

1

K

1

M

0

F

2

P

2

S

2

T

1

W

1

Y

2

V

Amino Acid Distribution

A: 2 R: 0 N: 4 D: 0 C: 6 E: 1 Q: 0 G: 2 H: 0 I: 3 L: 1 K: 1 M: 1 F: 0 P: 2 S: 2 T: 2 W: 1 Y: 1 V: 2

Length

31

Formula

C138H219N37O43S7

Mass

3308.90

PI

5.90

Net Charge

-0

Boman Index

0.00

Hydrophobicity

0.503

Evidence 1 Activity

Activity

Activity Endpoint HD50

Detail Source Activity Hemolysis

Activity Measurement 25 µM

Hemolysis Raw

Human erythrocytes: HD50>25 µM

Target

RBC Target Human erythrocytes

Source & Reference

Database Source Hemolytik PD_Hemolysis

Source DRAMP4

Reference

J Biol Chem. 2012 May 18 287(21):17598-17607.

Other

Source Activity Label	Source Definition
Hemolysis	Hemolytik experimentally validated hemolytic peptide annotation.

Evidence 2 Activity

Activity

Activity Endpoint 50% Hemolysis

Detail Source Activity Toxicity

Activity Measurement >25 µM

Toxicity Type Hemolytic Cytotoxic

Toxicity Raw

50% Hemolysis | >25 | µM | target cell: Human erythrocytes

Target

Target Cell Human erythrocytes

Source & Reference

Database Source NA

Source Database DBAASP

Reference

Novel cyclotides and uncyclotides with highly shortened precursors from Chassalia chartacea and effects of methionine oxidation on bioactivities. | J Biol Chem | 2012

Evidence 3 Activity

Activity

Activity Endpoint HD50

Detail Source Activity Toxicity

Activity Measurement 25 µM

Toxicity Type Hemolytic Cytotoxic

Toxicity Raw

Hemolytic_activity: Human erythrocytes: HD50>25 µM | Cytotoxicity: Not available

Target

Target Cell erythrocytes

Source & Reference

Database Source NA

Source Database DRAMP

Reference

J Biol Chem. 2012 May 18 287(21):17598-17607.

Evidence 4 Hemolysis

Activity

Activity Measurement 25 µM

Activity Endpoint HD50

Hemolysis Raw

Human erythrocytes: HD50>25 µM

Detail Source Activity Hemolysis

Target

RBC Target Human erythrocytes

Source & Reference

source HemoPI PD_Hemolysis

Reference

J Biol Chem. 2012 May 18 287(21):17598-17607.

Source DRAMP4

Database Source PD_Hemolysis