TPDB

3D PDB MODEL

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TPDB ID TPDB11250

Activity

Antibacterial Anticancer Antifungal Antiparasitic Antiviral Hemolysis Toxicity

Activity_category_statistics

Anti-infective peptides Cancer-related peptides Toxicity and safety peptides

Database_Source

AntiBP3 dbAMP DRAMP Peptipedia AFP-GFuse Antifungipept CAFPdb APD_complete HemoPI Hemolytic2 PD_Hemolysis ToxinPred 3.0

Sequence_Type

standard

Has_Modification No

Description A 30-aa standard natural multi-activity (Antibacterial, Anticancer, Antifungal, and other sources) peptide sequence curated from AntiBP3, dbAMP, DRAMP, and other sources, with an available 3D structural model.

Sequence

GAFGNFLKGVAKKAGLKILSIAQCKLSGTC

FASTA

>TPDB11250|Antibacterial;Anticancer;Antifungal;Antiparasitic;Antiviral;Hemolysis;Toxicity|length=30
GAFGNFLKGVAKKAGLKILSIAQCKLSGTC

Formula C136H231N37O36S2

Absent Amino Acids RDEHMPWY

Common Amino Acids GK

Mass 3024.68

PI 10.39

Basic Residues 5

Acidic Residues 0

Hydrophobic Residues 13

Net Charge +5

Boman Index 0.19

Hydrophobicity 0.513

Aliphatic Index 101.00

Half Life

Mammalian: 30 hour Yeast: >20 hour E.coli: >10 hour

Extinction Coefficient Cystines 125

Absorbance 280nm 4.13

Polar Residues 7

number

4

A

0

R

1

N

0

D

2

C

0

E

1

Q

5

G

0

H

2

I

4

L

5

K

0

M

2

F

0

P

2

S

1

T

0

W

0

Y

1

V

Amino Acid Distribution

A: 4 R: 0 N: 1 D: 0 C: 2 E: 0 Q: 1 G: 5 H: 0 I: 2 L: 4 K: 5 M: 0 F: 2 P: 0 S: 2 T: 1 W: 0 Y: 0 V: 1

Length

30

Formula

C136H231N37O36S2

Mass

3024.68

PI

10.39

Net Charge

+5

Boman Index

0.19

Hydrophobicity

0.513

Evidence 1 Activity

Activity

Activity Endpoint % Hemolysis

Detail Source Activity Hemolysis

Activity Measurement 17 %

Hemolysis Raw

[Ref.21171145] It has 17% hemolysis at 100 µg/ml against rabbit red blood cell

Source & Reference

Database Source Hemolytik APD_complete PD_Hemolysis

Source DRAMP4

Reference

J Pept Sci. 2011 Jan 17(1):68-72.

Other

Source Activity Label	Source Definition
Hemolysis	APD_complete APD-derived hemolytic peptide annotation. Hemolytik experimentally validated hemolytic peptide annotation.

Evidence 2 Activity

Activity

Activity Endpoint 17% Hemolysis

Detail Source Activity Toxicity

Activity Measurement 100 µg/mL

Toxicity Type Hemolytic Cytotoxic

Toxicity Raw

17% Hemolysis | 100 | µg/mL | target cell: Rabbit erythrocytes

Target

Target Cell Rabbit erythrocytes

Source & Reference

Database Source NA

Source Database DBAASP

Reference

Two novel antimicrobial peptides from skin secretions of the frog, Rana nigrovittata. | J Pept Sci | 2011

Evidence 3 Activity

Activity

Activity Endpoint % Hemolysis

Detail Source Activity Toxicity

Activity Measurement 17 %

Toxicity Type Hemolytic

Toxicity Raw

Hemolytic_activity: [Ref.21171145] It has 17% hemolysis at 100 µg/ml against rabbit red blood cell

Target

Target Cell erythrocytes

Source & Reference

Database Source NA

Source Database DRAMP

Reference

J Pept Sci. 2011 Jan 17(1):68-72.

Evidence 4 Hemolysis

Activity

Activity Measurement 17 %

Activity Endpoint % Hemolysis

Hemolysis Raw

[Ref.21171145] It has 17% hemolysis at 100 µg/ml against rabbit red blood cell

Detail Source Activity Hemolysis

Source & Reference

source APD_complete HemoPI PD_Hemolysis

Reference

J Pept Sci. 2011 Jan 17(1):68-72.

Source DRAMP4

Database Source APD_complete PD_Hemolysis

Other

Source Activity Label	Source Definition
Hemolysis	APD_complete APD-derived hemolytic peptide annotation.