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Peptide record

TPDB11244

Cycloviolacin O2 Antibacterial Anticancer Antifungal Antiparasitic Hemolysis Toxicity standard
30 amino acids
Basic Information
3D PDB MODEL
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TPDB11244
Cycloviolacin O2
Antibacterial Anticancer Antifungal Antiparasitic Hemolysis Toxicity
Anti-infective peptides Cancer-related peptides Toxicity and safety peptides
AntiBP3 dbAMP Peptipedia ACP740 _ACP240 AntiCP 2.0 CancerPPD 2.0 SATPdb dbACP iACP-DRLF Antifungipept CAFPdb dbAMP 3.0 APD_complete HemoPI Hemolytic2 PD_Hemolysis ToxinPred 3.0
standard
No
A 30-aa standard natural multi-activity (Antibacterial, Anticancer, Antifungal, and other sources) peptide sequence curated from AntiBP3, dbAMP, Peptipedia, and other sources, with an available 3D structural model.
Sequence
GIPCGESCVWIPCISSAIGCSCKSKVCYRN
Physicochemical Analysis
C133H215N37O40S6
DQHLMFT
C
3164.75
8.02
3
1
9
+2
1.31
0.443
74.67
Mammalian: 30 hour Yeast: >20 hour E.coli: >10 hour
7365
232.72
13
Residue Composition
number
1
A
1
R
1
N
0
D
6
C
1
E
0
Q
3
G
0
H
4
I
0
L
2
K
0
M
0
F
2
P
5
S
0
T
1
W
1
Y
2
V
Amino Acid Distribution
A: 1 R: 1 N: 1 D: 0 C: 6 E: 1 Q: 0 G: 3 H: 0 I: 4 L: 0 K: 2 M: 0 F: 0 P: 2 S: 5 T: 0 W: 1 Y: 1 V: 2
Chemical Descriptors
30
C133H215N37O40S6
3164.75
8.02
+2
1.31
0.443
Free
L
Not Available
Free
Antibacterial
Viola odorata
Evidence Records 35 records
Evidence 1 Activity

Activity

MIC
Antibacterial
20-25 µM

Target

Staphylococcus aureus

Source & Reference

dbAMP

Other

Source Activity Label Source Definition
Antibacterial
dbAMP classified as Antibacterial under the Anti-bacterial function class.
and E. coli in RDAs and MIC assays ( Pränting M et al., 2010 ). Moderately active against S. aureus (MIC 20-25 uM) and reduced bacterial load in vivo ( Fensterseifer IC et al., 2014 ). Also, MIC against E
Evidence 2 Activity

Activity

MIC
Antibacterial
4.2 µM

Target

A. baumannii

Source & Reference

dbAMP

Other

i (20 uM), P. aeruginosa (10 uM), and C. albicans (20 uM) ( Strömstedt AA et al., 2017 ). A. baumannii (MIC 4.2 uM) and B. subtilis (MIC 2.1 uM) (ref 2024). Also, plant fungi F. oxysporum (MIC 6-12.5 uM),
Evidence 3 Activity

Activity

MIC
Antibacterial
2.1 µM

Target

B. subtilis

Source & Reference

dbAMP

Other

M), and C. albicans (20 uM) ( Strömstedt AA et al., 2017 ). A. baumannii (MIC 4.2 uM) and B. subtilis (MIC 2.1 uM) (ref 2024). Also, plant fungi F. oxysporum (MIC 6-12.5 uM), B. cinerea (MIC 1.5 uM), F. g
Evidence 4 Activity

Activity

MIC
Antibacterial
6-12.5 µM

Target

F. oxysporum

Source & Reference

dbAMP

Other

7 ). A. baumannii (MIC 4.2 uM) and B. subtilis (MIC 2.1 uM) (ref 2024). Also, plant fungi F. oxysporum (MIC 6-12.5 uM), B. cinerea (MIC 1.5 uM), F. graminearum (MIC 6.25 uM), F. culmorum (MIC 1.5 uM), C. utre
Evidence 5 Activity

Activity

MIC
Antibacterial
1.5 µM

Target

B. cinerea

Source & Reference

dbAMP

Other

and B. subtilis (MIC 2.1 uM) (ref 2024). Also, plant fungi F. oxysporum (MIC 6-12.5 uM), B. cinerea (MIC 1.5 uM), F. graminearum (MIC 6.25 uM), F. culmorum (MIC 1.5 uM), C. utrechtense (MIC 6.25 uM), A.
Evidence 6 Activity

Activity

MIC
Antibacterial
6.25 µM

Target

F. graminearum

Source & Reference

dbAMP

Other

uM) (ref 2024). Also, plant fungi F. oxysporum (MIC 6-12.5 uM), B. cinerea (MIC 1.5 uM), F. graminearum (MIC 6.25 uM), F. culmorum (MIC 1.5 uM), C. utrechtense (MIC 6.25 uM), A. alternatam (MIC 1.5 uM) ( Sla
Evidence 7 Activity

Activity

MIC
Antibacterial
1.5 µM

Target

F. culmorum

Source & Reference

dbAMP

Other

ungi F. oxysporum (MIC 6-12.5 uM), B. cinerea (MIC 1.5 uM), F. graminearum (MIC 6.25 uM), F. culmorum (MIC 1.5 uM), C. utrechtense (MIC 6.25 uM), A. alternatam (MIC 1.5 uM) ( Slazak et al. 2018 ). Active
Evidence 8 Activity

Activity

MIC
Antibacterial
6.25 µM

Target

C. utrechtense

Source & Reference

dbAMP

Other

2.5 uM), B. cinerea (MIC 1.5 uM), F. graminearum (MIC 6.25 uM), F. culmorum (MIC 1.5 uM), C. utrechtense (MIC 6.25 uM), A. alternatam (MIC 1.5 uM) ( Slazak et al. 2018 ). Active against nematode parasites (ne
Evidence 9 Activity

Activity

MIC
Antibacterial
1.5 µM

Target

A. alternatam

Source & Reference

dbAMP

Other

M), F. graminearum (MIC 6.25 uM), F. culmorum (MIC 1.5 uM), C. utrechtense (MIC 6.25 uM), A. alternatam (MIC 1.5 uM) ( Slazak et al. 2018 ). Active against nematode parasites (nematocidal) of sheep (e.g. Ha
Evidence 10 Activity

Activity

Not Available
Anticancer

Target

Not Available
Not Available
Not Available

Assay & Model

Not Available
Not Available

Source & Reference

dbAMP 3.0 CancerPPD 2.0 dbACP SATPdb
CancerPPD2
1999

Other

Source Activity Label Source Definition
Anticancer
CancerPPD 2.0 manually curated experimentally verified anticancer peptide/protein annotation. dbAMP 3.0 classified as Anticancer under the disease-associated peptide function classes.
Evidence 11 Activity

Activity

MIC
Antifungal
20-25 µM

Target

Staphylococcus aureus

Source & Reference

dbAMP CAFPdb Antifungipept

Other

Source Activity Label Source Definition
Antifungal
dbAMP classified under the Anti-fungal function class.
and E. coli in RDAs and MIC assays ( Pränting M et al., 2010 ). Moderately active against S. aureus (MIC 20-25 uM) and reduced bacterial load in vivo ( Fensterseifer IC et al., 2014 ). Also, MIC against E
Evidence 12 Activity

Activity

MIC
Antifungal
4.2 µM

Target

A. baumannii

Source & Reference

dbAMP CAFPdb Antifungipept

Other

i (20 uM), P. aeruginosa (10 uM), and C. albicans (20 uM) ( Strömstedt AA et al., 2017 ). A. baumannii (MIC 4.2 uM) and B. subtilis (MIC 2.1 uM) (ref 2024). Also, plant fungi F. oxysporum (MIC 6-12.5 uM),
Evidence 13 Activity

Activity

MIC
Antifungal
2.1 µM

Target

B. subtilis

Source & Reference

dbAMP CAFPdb Antifungipept

Other

M), and C. albicans (20 uM) ( Strömstedt AA et al., 2017 ). A. baumannii (MIC 4.2 uM) and B. subtilis (MIC 2.1 uM) (ref 2024). Also, plant fungi F. oxysporum (MIC 6-12.5 uM), B. cinerea (MIC 1.5 uM), F. g
Evidence 14 Activity

Activity

MIC
Antifungal
6-12.5 µM

Target

F. oxysporum

Source & Reference

dbAMP CAFPdb Antifungipept

Other

7 ). A. baumannii (MIC 4.2 uM) and B. subtilis (MIC 2.1 uM) (ref 2024). Also, plant fungi F. oxysporum (MIC 6-12.5 uM), B. cinerea (MIC 1.5 uM), F. graminearum (MIC 6.25 uM), F. culmorum (MIC 1.5 uM), C. utre
Evidence 15 Activity

Activity

MIC
Antifungal
1.5 µM

Target

B. cinerea

Source & Reference

dbAMP CAFPdb Antifungipept

Other

and B. subtilis (MIC 2.1 uM) (ref 2024). Also, plant fungi F. oxysporum (MIC 6-12.5 uM), B. cinerea (MIC 1.5 uM), F. graminearum (MIC 6.25 uM), F. culmorum (MIC 1.5 uM), C. utrechtense (MIC 6.25 uM), A.
Evidence 16 Activity

Activity

MIC
Antifungal
6.25 µM

Target

F. graminearum

Source & Reference

dbAMP CAFPdb Antifungipept

Other

uM) (ref 2024). Also, plant fungi F. oxysporum (MIC 6-12.5 uM), B. cinerea (MIC 1.5 uM), F. graminearum (MIC 6.25 uM), F. culmorum (MIC 1.5 uM), C. utrechtense (MIC 6.25 uM), A. alternatam (MIC 1.5 uM) ( Sla
Evidence 17 Activity

Activity

MIC
Antifungal
1.5 µM

Target

F. culmorum

Source & Reference

dbAMP CAFPdb Antifungipept

Other

ungi F. oxysporum (MIC 6-12.5 uM), B. cinerea (MIC 1.5 uM), F. graminearum (MIC 6.25 uM), F. culmorum (MIC 1.5 uM), C. utrechtense (MIC 6.25 uM), A. alternatam (MIC 1.5 uM) ( Slazak et al. 2018 ). Active
Evidence 18 Activity

Activity

MIC
Antifungal
6.25 µM

Target

C. utrechtense

Source & Reference

dbAMP CAFPdb Antifungipept

Other

2.5 uM), B. cinerea (MIC 1.5 uM), F. graminearum (MIC 6.25 uM), F. culmorum (MIC 1.5 uM), C. utrechtense (MIC 6.25 uM), A. alternatam (MIC 1.5 uM) ( Slazak et al. 2018 ). Active against nematode parasites (ne
Evidence 19 Activity

Activity

MIC
Antifungal
1.5 µM

Target

A. alternatam

Source & Reference

dbAMP CAFPdb Antifungipept

Other

M), F. graminearum (MIC 6.25 uM), F. culmorum (MIC 1.5 uM), C. utrechtense (MIC 6.25 uM), A. alternatam (MIC 1.5 uM) ( Slazak et al. 2018 ). Active against nematode parasites (nematocidal) of sheep (e.g. Ha
Evidence 20 Activity

Activity

MIC
Antiparasitic
20-25 µM

Target

Staphylococcus aureus

Source & Reference

dbAMP 3.0

Other

Source Activity Label Source Definition
Antiparasitic
dbAMP 3.0 classified under the Anti-parasitic function class.
and E. coli in RDAs and MIC assays ( Pränting M et al., 2010 ). Moderately active against S. aureus (MIC 20-25 uM) and reduced bacterial load in vivo ( Fensterseifer IC et al., 2014 ). Also, MIC against E
Evidence 21 Activity

Activity

MIC
Antiparasitic
4.2 µM

Target

A. baumannii

Source & Reference

dbAMP 3.0

Other

i (20 uM), P. aeruginosa (10 uM), and C. albicans (20 uM) ( Strömstedt AA et al., 2017 ). A. baumannii (MIC 4.2 uM) and B. subtilis (MIC 2.1 uM) (ref 2024). Also, plant fungi F. oxysporum (MIC 6-12.5 uM),
Evidence 22 Activity

Activity

MIC
Antiparasitic
2.1 µM

Target

B. subtilis

Source & Reference

dbAMP 3.0

Other

M), and C. albicans (20 uM) ( Strömstedt AA et al., 2017 ). A. baumannii (MIC 4.2 uM) and B. subtilis (MIC 2.1 uM) (ref 2024). Also, plant fungi F. oxysporum (MIC 6-12.5 uM), B. cinerea (MIC 1.5 uM), F. g
Evidence 23 Activity

Activity

MIC
Antiparasitic
6-12.5 µM

Target

F. oxysporum

Source & Reference

dbAMP 3.0

Other

7 ). A. baumannii (MIC 4.2 uM) and B. subtilis (MIC 2.1 uM) (ref 2024). Also, plant fungi F. oxysporum (MIC 6-12.5 uM), B. cinerea (MIC 1.5 uM), F. graminearum (MIC 6.25 uM), F. culmorum (MIC 1.5 uM), C. utre
Evidence 24 Activity

Activity

MIC
Antiparasitic
1.5 µM

Target

B. cinerea

Source & Reference

dbAMP 3.0

Other

and B. subtilis (MIC 2.1 uM) (ref 2024). Also, plant fungi F. oxysporum (MIC 6-12.5 uM), B. cinerea (MIC 1.5 uM), F. graminearum (MIC 6.25 uM), F. culmorum (MIC 1.5 uM), C. utrechtense (MIC 6.25 uM), A.
Evidence 25 Activity

Activity

MIC
Antiparasitic
6.25 µM

Target

F. graminearum

Source & Reference

dbAMP 3.0

Other

uM) (ref 2024). Also, plant fungi F. oxysporum (MIC 6-12.5 uM), B. cinerea (MIC 1.5 uM), F. graminearum (MIC 6.25 uM), F. culmorum (MIC 1.5 uM), C. utrechtense (MIC 6.25 uM), A. alternatam (MIC 1.5 uM) ( Sla
Evidence 26 Activity

Activity

MIC
Antiparasitic
1.5 µM

Target

F. culmorum

Source & Reference

dbAMP 3.0

Other

ungi F. oxysporum (MIC 6-12.5 uM), B. cinerea (MIC 1.5 uM), F. graminearum (MIC 6.25 uM), F. culmorum (MIC 1.5 uM), C. utrechtense (MIC 6.25 uM), A. alternatam (MIC 1.5 uM) ( Slazak et al. 2018 ). Active
Evidence 27 Activity

Activity

MIC
Antiparasitic
6.25 µM

Target

C. utrechtense

Source & Reference

dbAMP 3.0

Other

2.5 uM), B. cinerea (MIC 1.5 uM), F. graminearum (MIC 6.25 uM), F. culmorum (MIC 1.5 uM), C. utrechtense (MIC 6.25 uM), A. alternatam (MIC 1.5 uM) ( Slazak et al. 2018 ). Active against nematode parasites (ne
Evidence 28 Activity

Activity

MIC
Antiparasitic
1.5 µM

Target

A. alternatam

Source & Reference

dbAMP 3.0

Other

M), F. graminearum (MIC 6.25 uM), F. culmorum (MIC 1.5 uM), C. utrechtense (MIC 6.25 uM), A. alternatam (MIC 1.5 uM) ( Slazak et al. 2018 ). Active against nematode parasites (nematocidal) of sheep (e.g. Ha
Evidence 29 Activity

Activity

% Hemolysis
Hemolysis
35 60 %
[Ref:16872274] It has 35% hemolytic activity at 1.0 µM and 60% hemolytic activity at 1.5 µM against human type A red blood cells

Target

human type A red blood cells

Source & Reference

Hemolytik APD_complete PD_Hemolysis
DRAMP4
Biochem J. 2006 Nov 15 400(1):1-12.##J Mol Biol. 1999 Dec 17 294(5):1327-1336.##Biochem J . 2006 Nov 15 400(1):1-12.

Other

Source Activity Label Source Definition
Hemolysis
APD_complete APD-derived hemolytic peptide annotation. Hemolytik experimentally validated hemolytic peptide annotation.
Evidence 30 Activity

Activity

IC50
Toxicity
0.87 µM
Cytotoxic
IC50 | 0.87 | µM | target cell: Human lymphocytes

Target

Human lymphocytes

Source & Reference

NA
DBAASP
Cyclotides: a novel type of cytotoxic agents | Mol Cancer Ther | 2002
Evidence 31 Activity

Activity

50% Hemolysis
Toxicity
5.1±0.1 µM
Hemolytic Cytotoxic
50% Hemolysis | 5.1±0.1 | µM | target cell: Human erythrocytes

Target

Human erythrocytes

Source & Reference

NA
DBAASP
Anticancer and toxic properties of cyclotides are dependent on phosphatidylethanolamine phospholipid targeting | Chembiochem | 2014
Evidence 32 Activity

Activity

50% Cell death
Toxicity
1.4±0.2 µM
Cytotoxic
50% Cell death | 1.4±0.2 | µM | target cell: Human PBMC

Target

Human PBMC

Source & Reference

NA
DBAASP
Anticancer and toxic properties of cyclotides are dependent on phosphatidylethanolamine phospholipid targeting | Chembiochem | 2014
Evidence 33 Activity

Activity

50% Cell death
Toxicity
3.1±0.2 µM
Cytotoxic
50% Cell death | 3.1±0.2 | µM | target cell: Human foreskin fibroblasts (HFF)

Target

Human foreskin fibroblasts (HFF)

Source & Reference

NA
DBAASP
Anticancer and toxic properties of cyclotides are dependent on phosphatidylethanolamine phospholipid targeting | Chembiochem | 2014
Evidence 34 Activity

Activity

IC50
Toxicity
17.05 µg/mL
Hemolytic Cytotoxic
Hemolytic_activity: [Ref:16872274] It has 35% hemolytic activity at 1.0 µM and 60% hemolytic activity at 1.5 µM against human type A red blood cells | Cytotoxicity: [Ref.20580652] Cytotoxicity: U251(IC50=17.05µg/mL), MDA-MB-231(IC50=4.81µg/mL), A549(IC50=5.99µg/mL), DU145(IC50=5.08µg/mL), BEL-7402(IC5=6.07µg/mL).

Target

erythrocytes

Source & Reference

NA
DRAMP
Biochem J. 2006 Nov 15 400(1):1-12.##J Mol Biol. 1999 Dec 17 294(5):1327-1336.##Biochem J . 2006 Nov 15 400(1):1-12.
Evidence 35 Hemolysis

Activity

35 60 %
% Hemolysis
[Ref:16872274] It has 35% hemolytic activity at 1.0 µM and 60% hemolytic activity at 1.5 µM against human type A red blood cells
Hemolysis

Target

human type A red blood cells

Source & Reference

APD_complete HemoPI PD_Hemolysis
Biochem J. 2006 Nov 15 400(1):1-12.##J Mol Biol. 1999 Dec 17 294(5):1327-1336.##Biochem J . 2006 Nov 15 400(1):1-12.
DRAMP4
APD_complete PD_Hemolysis

Other

Source Activity Label Source Definition
Hemolysis
APD_complete APD-derived hemolytic peptide annotation.