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Peptide record

TPDB11077

Chassatide C8 Antibacterial Anticancer Antifungal Hemolysis Toxicity standard

29 amino acids

3D PDB MODEL

Drag to rotate. Click a residue or atom to inspect it; the selected residue is highlighted in amber.

TPDB ID TPDB11077

Peptide_Name

Chassatide C8

Activity

Antibacterial Anticancer Antifungal Hemolysis Toxicity

Activity_category_statistics

Anti-infective peptides Cancer-related peptides Toxicity and safety peptides

Database_Source

AntiBP3 dbAMP DRAMP Peptipedia ACP740 _ACP240 AntiCP 2.0 CancerPPD 2.0 SATPdb dbACP iACP-DRLF Antifungipept APD_complete APD_hemolytic HemoPI Hemolytic2 PD_Hemolysis ToxinPred 3.0

Sequence_Type

standard

Has_Modification No

Description A 29-aa standard natural multi-activity (Antibacterial, Anticancer, Antifungal, and other sources) peptide sequence curated from AntiBP3, dbAMP, DRAMP, and other sources, with an available 3D structural model.

Sequence

AIPCGESCVWIPCISTVIGCSCSNKVCYR

FASTA

>TPDB11077|Antibacterial;Anticancer;Antifungal;Hemolysis;Toxicity|length=29
AIPCGESCVWIPCISTVIGCSCSNKVCYR

Formula C131H211N35O39S6

Absent Amino Acids DQHLMF

Common Amino Acids C

Mass 3092.69

PI 7.62

Basic Residues 2

Acidic Residues 1

Hydrophobic Residues 10

Net Charge +1

Boman Index 0.39

Hydrophobicity 0.755

Aliphatic Index 87.24

Half Life

Mammalian: 4.4 hour Yeast: >20 hour E.coli: >10 hour

Extinction Coefficient Cystines 7365

Absorbance 280nm 238.14

Polar Residues 13

number

Amino Acid Distribution

A: 1 R: 1 N: 1 D: 0 C: 6 E: 1 Q: 0 G: 2 H: 0 I: 4 L: 0 K: 1 M: 0 F: 0 P: 2 S: 4 T: 1 W: 1 Y: 1 V: 3

Length

Formula

C131H211N35O39S6

Mass

3092.69

7.62

Net Charge

Boman Index

0.39

Hydrophobicity

0.755

C_terminal_Modification

Free

Chirality

Linear_or_Cyclic

Not Available

N_terminal_Modification

Free

Nature

Antibacterial

Origin

Chassalia chartacea

Evidence 1 Activity

Activity

Activity Endpoint MIC

Detail Source Activity Antibacterial

Activity Measurement 6.6 µM

Target

Target Organism Escherichia coli

Source & Reference

Database Source dbAMP DRAMP

Other

Source Activity Label	Source Definition
Antibacterial	dbAMP classified as Antibacterial under the Anti-bacterial function class. DRAMP antibacterial activity supported by MIC records against bacterial target organisms.

Evidence Text

Active against E. coli (MIC 6.6 uM), but no activity was detected till 80 uM against S-aureus and S-epidermis (MIC >80 uM).

Evidence 2 Activity

Activity

Activity Raw

Not Available

Detail Source Activity Anticancer

Target

Cancer Type Not Available

Cell Line Not Available

Tissue Affected Not Available

Assay & Model

Assay Not Available

Testing Time Not Available

Source & Reference

Database Source CancerPPD 2.0 dbACP SATPdb

Source CancerPPD2

Year 2012

Other

Source Activity Label	Source Definition
Anticancer	CancerPPD 2.0 manually curated experimentally verified anticancer peptide/protein annotation.

Evidence 3 Activity

Activity

Activity Endpoint MIC

Detail Source Activity Antifungal

Activity Measurement 6.6 µM

Target

Target Organism Escherichia coli

Source & Reference

Database Source dbAMP DRAMP Antifungipept

Other

Source Activity Label	Source Definition
Antifungal	dbAMP classified under the Anti-fungal function class. DRAMP antifungal activity supported by activity records against fungal target organisms.

Evidence Text

Active against E. coli (MIC 6.6 uM), but no activity was detected till 80 uM against S-aureus and S-epidermis (MIC >80 uM).

Evidence 4 Activity

Activity

Activity Endpoint HD50

Detail Source Activity Hemolysis

Activity Measurement 25.5 µM

Hemolysis Raw

[Ref.22467870] HD50 = 25.5 µM against human red blood cells (Type A). Kalata B1 and melittin were used as the positive control.

Target

RBC Target human red blood cells

Source & Reference

Database Source Hemolytik APD_complete APD_hemolytic PD_Hemolysis

Source DRAMP4

Reference

J Biol Chem. 2012 May 18 287(21):17598-17607.

Other

Source Activity Label	Source Definition
Hemolysis	APD_complete APD-derived hemolytic peptide annotation. APD_hemolytic APD hemolytic peptide annotation. Hemolytik experimentally validated hemolytic peptide annotation.

Evidence 5 Activity

Activity

Activity Endpoint 50% Hemolysis

Detail Source Activity Toxicity

Activity Measurement 25.5 µM

Toxicity Type Hemolytic Cytotoxic

Toxicity Raw

50% Hemolysis | 25.5 | µM | target cell: Human erythrocytes

Target

Target Cell Human erythrocytes

Source & Reference

Database Source NA

Source Database DBAASP

Reference

Novel cyclotides and uncyclotides with highly shortened precursors from Chassalia chartacea and effects of methionine oxidation on bioactivities. | J Biol Chem | 2012

Evidence 6 Activity

Activity

Activity Endpoint HD50

Detail Source Activity Toxicity

Activity Measurement 25.5 µM

Toxicity Type Hemolytic Cytotoxic

Toxicity Raw

Hemolytic_activity: [Ref.22467870] HD50 = 25.5 µM against human red blood cells (Type A). Kalata B1 and melittin were used as the positive control. | Cytotoxicity: [Ref.22467870] IC50 = 1.0 µM targeting on HeLa cells. Kalata B1 was used as the positive control.

Target

Target Cell erythrocytes

Source & Reference

Database Source NA

Source Database DRAMP

Reference

J Biol Chem. 2012 May 18 287(21):17598-17607.

Evidence 7 Hemolysis

Activity

Activity Measurement 25.5 µM

Activity Endpoint HD50

Hemolysis Raw

[Ref.22467870] HD50 = 25.5 µM against human red blood cells (Type A). Kalata B1 and melittin were used as the positive control.

Detail Source Activity Hemolysis

Target

RBC Target human red blood cells

Source & Reference

source APD_complete APD_hemolytic HemoPI PD_Hemolysis

Reference

J Biol Chem. 2012 May 18 287(21):17598-17607.

Source DRAMP4

Database Source APD_complete APD_hemolytic PD_Hemolysis

Other

Source Activity Label	Source Definition
Hemolysis	APD_complete APD-derived hemolytic peptide annotation. APD_hemolytic APD hemolytic peptide annotation.