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Peptide record

TPDB10998

Antibacterial Hemolysis Toxicity standard

28 amino acids

3D PDB MODEL

Drag to rotate. Click a residue or atom to inspect it; the selected residue is highlighted in amber.

TPDB ID TPDB10998

Activity

Antibacterial Hemolysis Toxicity

Activity_category_statistics

Anti-infective peptides Toxicity and safety peptides

Database_Source

AntiBP3 dbAMP DRAMP Peptipedia PD_Hemolysis

Sequence_Type

standard

Has_Modification No

Description A 28-aa standard natural multi-activity (Antibacterial, Hemolysis, and Toxicity) peptide sequence curated from AntiBP3, dbAMP, DRAMP, and other sources, with an available 3D structural model.

Sequence

RVKRFKKFFRKIKKGFRKIFKKTKIFIG

FASTA

>TPDB10998|Antibacterial;Hemolysis;Toxicity|length=28
RVKRFKKFFRKIKKGFRKIFKKTKIFIG

Formula C175H290N50O30

Absent Amino Acids ANDCEQHLMPSWY

Common Amino Acids K

Mass 3574.55

PI 12.90

Basic Residues 14

Acidic Residues 0

Hydrophobic Residues 11

Net Charge +14

Boman Index 5.35

Hydrophobicity -0.696

Aliphatic Index 66.07

Half Life

Mammalian: 1 hour Yeast: 2 min E.coli: 2 min

Extinction Coefficient Cystines 0

Absorbance 280nm 0.00

Polar Residues 1

number

Amino Acid Distribution

A: 0 R: 4 N: 0 D: 0 C: 0 E: 0 Q: 0 G: 2 H: 0 I: 4 L: 0 K: 10 M: 0 F: 6 P: 0 S: 0 T: 1 W: 0 Y: 0 V: 1

Length

Formula

C175H290N50O30

Mass

3574.55

12.90

Net Charge

+14

Boman Index

5.35

Hydrophobicity

-0.696

Evidence 1 Activity

Activity

Activity Endpoint MIC

Detail Source Activity Antibacterial

Activity Measurement 2 µg/mL

Target

Target Organism Escherichia coli ATCC 25922

Source & Reference

Database Source dbAMP DRAMP

Other

Source Activity Label	Source Definition
Antibacterial	dbAMP classified as Antibacterial under the Anti-bacterial function class. DRAMP antibacterial activity supported by MIC records against bacterial target organisms.

Evidence Text

Active against Gram- E. coli ATCC 25922 (MIC 2 ug/ml), P. aeruginosa ATCC 27853 (MIC 3 ug/ml), S. typhimurium ATCC 14028 (MIC 1.5 ug/ml), Gram+

Evidence 2 Activity

Activity

Activity Endpoint MIC

Detail Source Activity Antibacterial

Activity Measurement 3 µg/mL

Target

Target Organism Pseudomonas aeruginosa ATCC 27853

Source & Reference

Database Source dbAMP DRAMP

Other

Evidence Text

Active against Gram- E. coli ATCC 25922 (MIC 2 ug/ml), P. aeruginosa ATCC 27853 (MIC 3 ug/ml), S. typhimurium ATCC 14028 (MIC 1.5 ug/ml), Gram+ S.aureus ATCC 29213 (MIC >128 ug/ml), B

Evidence 3 Activity

Activity

Activity Endpoint MIC

Detail Source Activity Antibacterial

Activity Measurement 1.5 µg/mL

Target

Target Organism S. typhimurium ATCC 14028

Source & Reference

Database Source dbAMP DRAMP

Other

Evidence Text

e against Gram- E. coli ATCC 25922 (MIC 2 ug/ml), P. aeruginosa ATCC 27853 (MIC 3 ug/ml), S. typhimurium ATCC 14028 (MIC 1.5 ug/ml), Gram+ S.aureus ATCC 29213 (MIC >128 ug/ml), B. cereus ATCC 10876 (MIC 46 ug/ml).

Evidence 4 Activity

Activity

Activity Endpoint MIC

Detail Source Activity Antibacterial

Activity Measurement >128 µg/mL

Target

Target Organism S.aureus ATCC 29213

Source & Reference

Database Source dbAMP DRAMP

Other

Evidence Text

P. aeruginosa ATCC 27853 (MIC 3 ug/ml), S. typhimurium ATCC 14028 (MIC 1.5 ug/ml), Gram+ S.aureus ATCC 29213 (MIC >128 ug/ml), B. cereus ATCC 10876 (MIC 46 ug/ml).

Evidence 5 Activity

Activity

Activity Endpoint MIC

Detail Source Activity Antibacterial

Activity Measurement 46 µg/mL

Target

Target Organism B. cereus ATCC 10876

Source & Reference

Database Source dbAMP DRAMP

Other

Evidence Text

), S. typhimurium ATCC 14028 (MIC 1.5 ug/ml), Gram+ S.aureus ATCC 29213 (MIC >128 ug/ml), B. cereus ATCC 10876 (MIC 46 ug/ml).

Evidence 6 Activity

Activity

Activity Endpoint % Hemolysis

Detail Source Activity Hemolysis

Activity Measurement 0.4 0.5 0.6 0.7 %

Hemolysis Raw

[Ref.28630199]1.4 ± 0.4% hemolysis at 8 µg/ml, 5.1 ± 0.5% hemolysis at 16 µg/ml, 7.3 ± 0.6% hemolysis at 32 µg/ml, 12.2 ± 0.7% hemolysis at 64 µg/ml against chicken red blood cells

Target

RBC Target chicken red blood cells

Source & Reference

Database Source Hemolytik PD_Hemolysis

Source DRAMP4

Reference

Antimicrob Agents Chemother. 2017 Aug 24 61(9). pii: e00530-17.

Other

Source Activity Label	Source Definition
Hemolysis	Hemolytik experimentally validated hemolytic peptide annotation.

Evidence 7 Activity

Activity

Activity Endpoint 0% Hemolysis

Detail Source Activity Toxicity

Activity Measurement 4 µg/mL

Toxicity Type Hemolytic Cytotoxic

Toxicity Raw

0% Hemolysis | 4 | µg/mL | target cell: Chicken erythrocytes

Target

Target Cell Chicken erythrocytes

Source & Reference

Database Source NA

Source Database DBAASP

Reference

Genomewide Analysis of the Antimicrobial Peptides in Python bivittatus and Characterization of Cathelicidins with Potent Antimicrobial Activity and Low Cytotoxicity. | Antimicrob Agents Chemother | 2017

Evidence 8 Activity

Activity

Activity Endpoint 1.4±0.4% Hemolysis

Detail Source Activity Toxicity

Activity Measurement 8 µg/mL

Toxicity Type Hemolytic Cytotoxic

Toxicity Raw

1.4±0.4% Hemolysis | 8 | µg/mL | target cell: Chicken erythrocytes

Target

Target Cell Chicken erythrocytes

Source & Reference

Database Source NA

Source Database DBAASP

Reference

Evidence 9 Activity

Activity

Activity Endpoint 5.1±0.5% Hemolysis

Detail Source Activity Toxicity

Activity Measurement 16 µg/mL

Toxicity Type Hemolytic Cytotoxic

Toxicity Raw

5.1±0.5% Hemolysis | 16 | µg/mL | target cell: Chicken erythrocytes

Target

Target Cell Chicken erythrocytes

Source & Reference

Database Source NA

Source Database DBAASP

Reference

Evidence 10 Activity

Activity

Activity Endpoint 7.3±0.6% Hemolysis

Detail Source Activity Toxicity

Activity Measurement 32 µg/mL

Toxicity Type Hemolytic Cytotoxic

Toxicity Raw

7.3±0.6% Hemolysis | 32 | µg/mL | target cell: Chicken erythrocytes

Target

Target Cell Chicken erythrocytes

Source & Reference

Database Source NA

Source Database DBAASP

Reference

Evidence 11 Activity

Activity

Activity Endpoint 12.2±0.7% Hemolysis

Detail Source Activity Toxicity

Activity Measurement 64 µg/mL

Toxicity Type Hemolytic Cytotoxic

Toxicity Raw

12.2±0.7% Hemolysis | 64 | µg/mL | target cell: Chicken erythrocytes

Target

Target Cell Chicken erythrocytes

Source & Reference

Database Source NA

Source Database DBAASP

Reference

Evidence 12 Activity

Activity

Activity Endpoint % Hemolysis

Detail Source Activity Toxicity

Activity Measurement 0.4 %

Toxicity Type Hemolytic

Toxicity Raw

Hemolytic_activity: [Ref.28630199]1.4 ± 0.4% hemolysis at 8 µg/ml, 5.1 ± 0.5% hemolysis at 16 µg/ml, 7.3 ± 0.6% hemolysis at 32 µg/ml, 12.2 ± 0.7% hemolysis at 64 µg/ml against chicken red blood cells

Target

Target Cell erythrocytes

Source & Reference

Database Source NA

Source Database DRAMP

Reference

Antimicrob Agents Chemother. 2017 Aug 24 61(9). pii: e00530-17.

Evidence 13 Hemolysis

Activity

Activity Measurement 0.4 0.5 0.6 0.7 %

Activity Endpoint % Hemolysis

Hemolysis Raw

[Ref.28630199]1.4 ± 0.4% hemolysis at 8 µg/ml, 5.1 ± 0.5% hemolysis at 16 µg/ml, 7.3 ± 0.6% hemolysis at 32 µg/ml, 12.2 ± 0.7% hemolysis at 64 µg/ml against chicken red blood cells

Detail Source Activity Hemolysis

Target

RBC Target chicken red blood cells

Source & Reference

source PD_Hemolysis

Reference

Antimicrob Agents Chemother. 2017 Aug 24 61(9). pii: e00530-17.

Source DRAMP4

Database Source PD_Hemolysis