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Peptide record

TPDB10852

Latarcin 2a Antibacterial Anticancer Antifungal Antiparasitic Hemolysis Toxicity standard

26 amino acids

3D PDB MODEL

Drag to rotate. Click a residue or atom to inspect it; the selected residue is highlighted in amber.

TPDB ID TPDB10852

Peptide_Name

Latarcin 2a

Activity

Antibacterial Anticancer Antifungal Antiparasitic Hemolysis Toxicity

Activity_category_statistics

Anti-infective peptides Cancer-related peptides Toxicity and safety peptides

Database_Source

AntiBP3 dbAMP DRAMP Peptipedia CancerPPD 2.0 SATPdb dbACP AFP-GFuse Antifungipept CAFPdb APD_complete APD_hemolytic HemoPI Hemolytic2 PD_Hemolysis ToxinPred 3.0

Sequence_Type

standard

Has_Modification No

Description A 26-aa standard natural multi-activity (Antibacterial, Anticancer, Antifungal, and other sources) peptide sequence curated from AntiBP3, dbAMP, DRAMP, and other sources, with an available 3D structural model.

Sequence

GLFGKLIKKFGRKAISYAVKKARGKH

FASTA

>TPDB10852|Antibacterial;Anticancer;Antifungal;Antiparasitic;Hemolysis;Toxicity|length=26
GLFGKLIKKFGRKAISYAVKKARGKH

Formula C136H229N41O29

Absent Amino Acids NDCEQMPTW

Common Amino Acids K

Mass 2902.57

PI 11.77

Basic Residues 10

Acidic Residues 0

Hydrophobic Residues 11

Net Charge +9

Boman Index 4.30

Hydrophobicity -0.438

Aliphatic Index 82.69

Half Life

Mammalian: 30 hour Yeast: >20 hour E.coli: >10 hour

Extinction Coefficient Cystines 1490

Absorbance 280nm 51.33

Polar Residues 2

number

Amino Acid Distribution

A: 3 R: 2 N: 0 D: 0 C: 0 E: 0 Q: 0 G: 4 H: 1 I: 2 L: 2 K: 7 M: 0 F: 2 P: 0 S: 1 T: 0 W: 0 Y: 1 V: 1

Length

Formula

C136H229N41O29

Mass

2902.57

11.77

Net Charge

Boman Index

4.30

Hydrophobicity

-0.438

C_terminal_Modification

Free

Chirality

Linear_or_Cyclic

Linear

N_terminal_Modification

Free

Nature

Antimicrobial

Origin

Central Asian spider, Lachesana tarabaevi

Evidence 1 Activity

Activity

Activity Endpoint MIC

Detail Source Activity Antibacterial

Activity Measurement 0.5-0.7 µM

Target

Target Organism Escherichia coli DH1

Source & Reference

Database Source dbAMP DRAMP

Other

Source Activity Label	Source Definition
Antibacterial	dbAMP classified as Antibacterial under the Anti-bacterial function class. DRAMP antibacterial activity supported by MIC records against bacterial target organisms.

Evidence Text

Active against Gram- E. coli DH5alpha, E. coli DH1 (MIC 0.5-0.7 uM), P. aeruginosa (MIC 6.7 uM), Gram+ A. globiformis, B. subtilis (MIC 0.4-0.7 uM), yeasts P

Evidence 2 Activity

Activity

Activity Endpoint MIC

Detail Source Activity Antibacterial

Activity Measurement 6.7 µM

Target

Target Organism Pseudomonas aeruginosa

Source & Reference

Database Source dbAMP DRAMP

Other

Evidence Text

Active against Gram- E. coli DH5alpha, E. coli DH1 (MIC 0.5-0.7 uM), P. aeruginosa (MIC 6.7 uM), Gram+ A. globiformis, B. subtilis (MIC 0.4-0.7 uM), yeasts P. pastoris (MIC 6.7 uM), S.

Evidence 3 Activity

Activity

Activity Endpoint MIC

Detail Source Activity Antibacterial

Activity Measurement 0.4-0.7 µM

Target

Target Organism B. subtilis

Source & Reference

Database Source dbAMP DRAMP

Other

Evidence Text

DH5alpha, E. coli DH1 (MIC 0.5-0.7 uM), P. aeruginosa (MIC 6.7 uM), Gram+ A. globiformis, B. subtilis (MIC 0.4-0.7 uM), yeasts P. pastoris (MIC 6.7 uM), S. cerevisiae (MIC 54 uM).

Evidence 4 Activity

Activity

Activity Endpoint MIC

Detail Source Activity Antibacterial

Activity Measurement 6.7 µM

Target

Target Organism P. pastoris

Source & Reference

Database Source dbAMP DRAMP

Other

Evidence Text

), P. aeruginosa (MIC 6.7 uM), Gram+ A. globiformis, B. subtilis (MIC 0.4-0.7 uM), yeasts P. pastoris (MIC 6.7 uM), S. cerevisiae (MIC 54 uM).

Evidence 5 Activity

Activity

Activity Endpoint MIC

Detail Source Activity Antibacterial

Activity Measurement 54 µM

Target

Target Organism S. cerevisiae

Source & Reference

Database Source dbAMP DRAMP

Other

Evidence Text

uM), Gram+ A. globiformis, B. subtilis (MIC 0.4-0.7 uM), yeasts P. pastoris (MIC 6.7 uM), S. cerevisiae (MIC 54 uM).

Evidence 6 Activity

Activity

Activity Raw

graph fig 8 A

Detail Source Activity Anticancer

Target

Cancer Type Breast Cancer

Cell Line Breast tumor cellline

Tissue Affected Breast

Assay & Model

Assay LDH leakage assay

Testing Time Not Available

Source & Reference

Database Source CancerPPD 2.0 dbACP SATPdb

Source CancerPPD2

Year 2012

Other

Source Activity Label	Source Definition
Anticancer	CancerPPD 2.0 manually curated experimentally verified anticancer peptide/protein annotation.

Evidence 7 Activity

Activity

Activity Endpoint MIC

Detail Source Activity Antifungal

Activity Measurement 0.5-0.7 µM

Target

Target Organism Escherichia coli DH1

Source & Reference

Database Source dbAMP DRAMP CAFPdb Antifungipept

Other

Source Activity Label	Source Definition
Antifungal	dbAMP classified under the Anti-fungal function class. DRAMP antifungal activity supported by activity records against fungal target organisms.

Evidence Text

Active against Gram- E. coli DH5alpha, E. coli DH1 (MIC 0.5-0.7 uM), P. aeruginosa (MIC 6.7 uM), Gram+ A. globiformis, B. subtilis (MIC 0.4-0.7 uM), yeasts P

Evidence 8 Activity

Activity

Activity Endpoint MIC

Detail Source Activity Antifungal

Activity Measurement 6.7 µM

Target

Target Organism Pseudomonas aeruginosa

Source & Reference

Database Source dbAMP DRAMP CAFPdb Antifungipept

Other

Evidence Text

Active against Gram- E. coli DH5alpha, E. coli DH1 (MIC 0.5-0.7 uM), P. aeruginosa (MIC 6.7 uM), Gram+ A. globiformis, B. subtilis (MIC 0.4-0.7 uM), yeasts P. pastoris (MIC 6.7 uM), S.

Evidence 9 Activity

Activity

Activity Endpoint MIC

Detail Source Activity Antifungal

Activity Measurement 0.4-0.7 µM

Target

Target Organism B. subtilis

Source & Reference

Database Source dbAMP DRAMP CAFPdb Antifungipept

Other

Evidence Text

DH5alpha, E. coli DH1 (MIC 0.5-0.7 uM), P. aeruginosa (MIC 6.7 uM), Gram+ A. globiformis, B. subtilis (MIC 0.4-0.7 uM), yeasts P. pastoris (MIC 6.7 uM), S. cerevisiae (MIC 54 uM).

Evidence 10 Activity

Activity

Activity Endpoint MIC

Detail Source Activity Antifungal

Activity Measurement 6.7 µM

Target

Target Organism P. pastoris

Source & Reference

Database Source dbAMP DRAMP CAFPdb Antifungipept

Other

Evidence Text

), P. aeruginosa (MIC 6.7 uM), Gram+ A. globiformis, B. subtilis (MIC 0.4-0.7 uM), yeasts P. pastoris (MIC 6.7 uM), S. cerevisiae (MIC 54 uM).

Evidence 11 Activity

Activity

Activity Endpoint MIC

Detail Source Activity Antifungal

Activity Measurement 54 µM

Target

Target Organism S. cerevisiae

Source & Reference

Database Source dbAMP DRAMP CAFPdb Antifungipept

Other

Evidence Text

uM), Gram+ A. globiformis, B. subtilis (MIC 0.4-0.7 uM), yeasts P. pastoris (MIC 6.7 uM), S. cerevisiae (MIC 54 uM).

Evidence 12 Activity

Activity

Activity Endpoint MIC

Detail Source Activity Antiparasitic

Activity Measurement 0.5-0.7 µM

Target

Target Organism Escherichia coli DH1

Source & Reference

Database Source DRAMP

Other

Source Activity Label	Source Definition
Antiparasitic	DRAMP antiparasitic activity supported by activity records against parasitic targets.

Evidence Text

Active against Gram- E. coli DH5alpha, E. coli DH1 (MIC 0.5-0.7 uM), P. aeruginosa (MIC 6.7 uM), Gram+ A. globiformis, B. subtilis (MIC 0.4-0.7 uM), yeasts P

Evidence 13 Activity

Activity

Activity Endpoint MIC

Detail Source Activity Antiparasitic

Activity Measurement 6.7 µM

Target

Target Organism Pseudomonas aeruginosa

Source & Reference

Database Source DRAMP

Other

Evidence Text

Active against Gram- E. coli DH5alpha, E. coli DH1 (MIC 0.5-0.7 uM), P. aeruginosa (MIC 6.7 uM), Gram+ A. globiformis, B. subtilis (MIC 0.4-0.7 uM), yeasts P. pastoris (MIC 6.7 uM), S.

Evidence 14 Activity

Activity

Activity Endpoint MIC

Detail Source Activity Antiparasitic

Activity Measurement 0.4-0.7 µM

Target

Target Organism B. subtilis

Source & Reference

Database Source DRAMP

Other

Evidence Text

DH5alpha, E. coli DH1 (MIC 0.5-0.7 uM), P. aeruginosa (MIC 6.7 uM), Gram+ A. globiformis, B. subtilis (MIC 0.4-0.7 uM), yeasts P. pastoris (MIC 6.7 uM), S. cerevisiae (MIC 54 uM).

Evidence 15 Activity

Activity

Activity Endpoint MIC

Detail Source Activity Antiparasitic

Activity Measurement 6.7 µM

Target

Target Organism P. pastoris

Source & Reference

Database Source DRAMP

Other

Evidence Text

), P. aeruginosa (MIC 6.7 uM), Gram+ A. globiformis, B. subtilis (MIC 0.4-0.7 uM), yeasts P. pastoris (MIC 6.7 uM), S. cerevisiae (MIC 54 uM).

Evidence 16 Activity

Activity

Activity Endpoint MIC

Detail Source Activity Antiparasitic

Activity Measurement 54 µM

Target

Target Organism S. cerevisiae

Source & Reference

Database Source DRAMP

Other

Evidence Text

uM), Gram+ A. globiformis, B. subtilis (MIC 0.4-0.7 uM), yeasts P. pastoris (MIC 6.7 uM), S. cerevisiae (MIC 54 uM).

Evidence 17 Activity

Activity

Activity Endpoint % Hemolysis

Detail Source Activity Hemolysis

Activity Measurement 20 %

Hemolysis Raw

[Ref.16735513]20% hemolytic activity at 6 µM against rabbit erythrocytes

Target

RBC Target rabbit erythrocytes

Source & Reference

Database Source Hemolytik APD_complete APD_hemolytic PD_Hemolysis

Source DRAMP4

Reference

J Biol Chem. 2006 Jul 28 281(30):20983-20992.##Biochemistry. 2006 Sep 5 45(35):10759-10767.

Other

Source Activity Label	Source Definition
Hemolysis	APD_complete APD-derived hemolytic peptide annotation. APD_hemolytic APD hemolytic peptide annotation. Hemolytik experimentally validated hemolytic peptide annotation.

Evidence 18 Activity

Activity

Activity Endpoint 20% Hemolysis

Detail Source Activity Toxicity

Activity Measurement 6.0 µM

Toxicity Type Hemolytic Cytotoxic

Toxicity Raw

20% Hemolysis | 6.0 | µM | target cell: Rabbit erythrocytes

Target

Target Cell Rabbit erythrocytes

Source & Reference

Database Source NA

Source Database DBAASP

Reference

Latarcins, antimicrobial and cytolytic peptides from the venom of the spider Lachesana tarabaevi (Zodariidae) that exemplify biomolecular diversity. | J Biol Chem | 2006

Evidence 19 Activity

Activity

Activity Endpoint 50% Hemolysis

Detail Source Activity Toxicity

Activity Measurement 6 µM

Toxicity Type Hemolytic Cytotoxic

Toxicity Raw

50% Hemolysis | 6 | µM | target cell: Human erythrocytes

Target

Target Cell Human erythrocytes

Source & Reference

Database Source NA

Source Database DBAASP

Reference

N-terminal amphipathic helix as a trigger of hemolytic activity in antimicrobial peptides: a case study in latarcins. | FEBS Lett | 2009

Evidence 20 Activity

Activity

Activity Endpoint 50% Cell death

Detail Source Activity Toxicity

Activity Measurement 18 µM

Toxicity Type Cytotoxic

Toxicity Raw

50% Cell death | 18 | µM | target cell: Human leukocytes

Target

Target Cell Human leukocytes

Source & Reference

Database Source NA

Source Database DBAASP

Reference

N-terminal amphipathic helix as a trigger of hemolytic activity in antimicrobial peptides: a case study in latarcins. | FEBS Lett | 2009

Evidence 21 Activity

Activity

Activity Endpoint 100% Hemolysis

Detail Source Activity Toxicity

Activity Measurement 256 µg/mL

Toxicity Type Hemolytic Cytotoxic

Toxicity Raw

100% Hemolysis | 256 | µg/mL | target cell: Human erythrocytes

Target

Target Cell Human erythrocytes

Source & Reference

Database Source NA

Source Database DBAASP

Reference

Membrane Interactions of Latarcins: Antimicrobial Peptides from Spider Venom | Int J Mol Sci | 2021

Evidence 22 Activity

Activity

Activity Endpoint 40% Hemolysis

Detail Source Activity Toxicity

Activity Measurement 100 µM

Toxicity Type Hemolytic Cytotoxic

Toxicity Raw

40% Hemolysis | 100 | µM | target cell: Human erythrocytes

Target

Target Cell Human erythrocytes

Source & Reference

Database Source NA

Source Database DBAASP

Reference

Potent antimicrobial peptides with selectivity for Bacillus anthracis over human erythrocytes. | Int J Antimicrob Agents | 2011

Evidence 23 Activity

Activity

Detail Source Activity Toxicity

Toxicity Type Hemolytic

Toxicity Raw

Hemolytic_activity: [Ref.16735513]20% hemolytic activity at 6 µM against rabbit erythrocytes

Target

Target Cell erythrocytes

Source & Reference

Database Source NA

Source Database DRAMP

Reference

J Biol Chem. 2006 Jul 28 281(30):20983-20992.##Biochemistry. 2006 Sep 5 45(35):10759-10767.

Evidence 24 Hemolysis

Activity

Activity Measurement 20 %

Activity Endpoint % Hemolysis

Hemolysis Raw

[Ref.16735513]20% hemolytic activity at 6 µM against rabbit erythrocytes

Detail Source Activity Hemolysis

Target

RBC Target rabbit erythrocytes

Source & Reference

source APD_complete APD_hemolytic HemoPI PD_Hemolysis

Reference

J Biol Chem. 2006 Jul 28 281(30):20983-20992.##Biochemistry. 2006 Sep 5 45(35):10759-10767.

Source DRAMP4

Database Source APD_complete APD_hemolytic PD_Hemolysis

Other

Source Activity Label	Source Definition
Hemolysis	APD_complete APD-derived hemolytic peptide annotation. APD_hemolytic APD hemolytic peptide annotation.