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Peptide record

TPDB10655

bLF Bovine Lactoferricin (LfcinB) Antibacterial Anticancer Antifungal Antiparasitic Antiviral Cell-penetrating Peptides Toxicity standard
25 amino acids
Basic Information
3D PDB MODEL
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TPDB10655
bLF Bovine Lactoferricin (LfcinB)
Antibacterial Anticancer Antifungal Antiparasitic Antiviral Cell-penetrating Peptides Toxicity
Anti-infective peptides Cancer-related peptides Toxicity and safety peptides Delivery and barrier-penetrating peptides
AntiBP3 dbAMP DRAMP Peptipedia ACP740 _ACP240 AntiCP 2.0 CancerPPD 2.0 SATPdb dbACP iACP-DRLF AFP-GFuse Antifungipept CAFPdb dbAMP 3.0 AI4AVP CPPsite3.0 ToxinPred 3.0
standard
No
A 25-aa standard natural multi-activity (Antibacterial, Anticancer, Antifungal, and other sources) peptide sequence curated from AntiBP3, dbAMP, DRAMP, and other sources, with an available 3D structural model.
Sequence
FKCRRWQWRMKKLGAPSITCVRRAF
Physicochemical Analysis
C141H226N46O29S3
NDEHY
R
3125.82
12.24
8
0
10
+8
0.52
-0.576
50.80
Mammalian: 1.1 hour Yeast: 3 min E.coli: 2 min
11125
355.91
5
Residue Composition
number
2
A
5
R
0
N
0
D
2
C
0
E
1
Q
1
G
0
H
1
I
1
L
3
K
1
M
2
F
1
P
1
S
1
T
2
W
0
Y
1
V
Amino Acid Distribution
A: 2 R: 5 N: 0 D: 0 C: 2 E: 0 Q: 1 G: 1 H: 0 I: 1 L: 1 K: 3 M: 1 F: 2 P: 1 S: 1 T: 1 W: 2 Y: 0 V: 1
Chemical Descriptors
25
C141H226N46O29S3
3125.82
12.24
+8
0.52
-0.576
Free
L
Cationic
Linear
Linear
Free
Antimicrobial
Bovine Milk Bos Taurus
N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CC1=C(C(=NC1)C)CC)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](C(C)C)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CS)C(=O)O
Evidence Records 29 records
Evidence 1 Activity

Activity

MIC
Antibacterial
2 µM

Target

Escherichia coli IID-861

Source & Reference

dbAMP DRAMP

Other

Source Activity Label Source Definition
Antibacterial
dbAMP classified as Antibacterial under the Anti-bacterial function class. DRAMP antibacterial activity supported by MIC records against bacterial target organisms.
Active against E. coli IID-861 (MIC 2 uM), S. enteritidis, K. pneumoniae JCM1662T (MIC 3 uM), P. vulgaris, Y. enterocolitica, P. ae
Evidence 2 Activity

Activity

MIC
Antibacterial
3 µM

Target

Klebsiella pneumoniae JCM1662T

Source & Reference

dbAMP DRAMP

Other

Active against E. coli IID-861 (MIC 2 uM), S. enteritidis, K. pneumoniae JCM1662T (MIC 3 uM), P. vulgaris, Y. enterocolitica, P. aeruginosa IFO-3446 (MIC 3 uM), C. jejuni, S. aureus
Evidence 3 Activity

Activity

MIC
Antibacterial
3 µM

Target

Pseudomonas aeruginosa IFO-3446

Source & Reference

dbAMP DRAMP

Other

2 uM), S. enteritidis, K. pneumoniae JCM1662T (MIC 3 uM), P. vulgaris, Y. enterocolitica, P. aeruginosa IFO-3446 (MIC 3 uM), C. jejuni, S. aureus JCM-2151 or MRSA (MIC 2 uM), S. mutans, C. diphtheriae, L. monocyto
Evidence 4 Activity

Activity

MIC
Antibacterial
2 µM

Target

Staphylococcus aureus JCM-2151 or MRSA

Source & Reference

dbAMP DRAMP

Other

(MIC 3 uM), P. vulgaris, Y. enterocolitica, P. aeruginosa IFO-3446 (MIC 3 uM), C. jejuni, S. aureus JCM-2151 or MRSA (MIC 2 uM), S. mutans, C. diphtheriae, L. monocytogenes IDF-1 b(MIC 0.3 uM) and Clostridium perfring
Evidence 5 Activity

Activity

MIC
Antibacterial
0.3 µM

Target

L. monocytogenes IDF-1 b

Source & Reference

dbAMP DRAMP

Other

(MIC 3 uM), C. jejuni, S. aureus JCM-2151 or MRSA (MIC 2 uM), S. mutans, C. diphtheriae, L. monocytogenes IDF-1 b(MIC 0.3 uM) and Clostridium perfringens. Also active against E. coli ATCC 2592, M. luteus, and S. cer
Evidence 6 Activity

Activity

~60% cell death at 300 µg/ml
Anticancer

Target

Breast Cancer
MDA-MB-468
Breast

Assay & Model

Annexin V-FITC assay
48-h

Source & Reference

dbAMP 3.0 CancerPPD 2.0 dbACP SATPdb
CancerPPD2
2021

Other

Source Activity Label Source Definition
Anticancer
CancerPPD 2.0 manually curated experimentally verified anticancer peptide/protein annotation. dbAMP 3.0 classified as Anticancer under the disease-associated peptide function classes.
Evidence 7 Activity

Activity

~80% cell death at 100 µg/ml
Anticancer

Target

Breast Cancer
SK-BR-3
Breast

Assay & Model

Annexin V-FITC assay
48-h

Source & Reference

dbAMP 3.0 CancerPPD 2.0 dbACP SATPdb
CancerPPD2
2021
Evidence 8 Activity

Activity

~50% cell death at 300 µg/ml
Anticancer

Target

Breast Cancer
MCF-7
Breast

Assay & Model

Annexin V-FITC assay
48-h

Source & Reference

dbAMP 3.0 CancerPPD 2.0 dbACP SATPdb
CancerPPD2
2021
Evidence 9 Activity

Activity

~80% cell death at 200 µg/ml
Anticancer

Target

Breast Cancer
MDA-MB-231
Breast

Assay & Model

Annexin V-FITC assay
48-h

Source & Reference

dbAMP 3.0 CancerPPD 2.0 dbACP SATPdb
CancerPPD2
2021
Evidence 10 Activity

Activity

cell viability
cell viability ~ 21% at 10 µM
Anticancer
~21 % at 10 µM

Target

Liver Cancer
HepG-2
Liver

Assay & Model

MTT assay
24-h

Source & Reference

dbAMP 3.0 CancerPPD 2.0 dbACP SATPdb
CancerPPD2
2023
Evidence 11 Activity

Activity

cell viability
cell viability ~ 17.5% at 20 µM
Anticancer
~17.5 % at 20 µM

Target

Blood Cancer
Jurkat
Blood

Assay & Model

MTT assay
4-h

Source & Reference

dbAMP 3.0 CancerPPD 2.0 dbACP SATPdb
CancerPPD2
2023
Evidence 12 Activity

Activity

MIC
Antifungal
2 µM

Target

Escherichia coli IID-861

Source & Reference

dbAMP DRAMP CAFPdb Antifungipept

Other

Source Activity Label Source Definition
Antifungal
dbAMP classified under the Anti-fungal function class. DRAMP antifungal activity supported by activity records against fungal target organisms.
Active against E. coli IID-861 (MIC 2 uM), S. enteritidis, K. pneumoniae JCM1662T (MIC 3 uM), P. vulgaris, Y. enterocolitica, P. ae
Evidence 13 Activity

Activity

MIC
Antifungal
3 µM

Target

Klebsiella pneumoniae JCM1662T

Source & Reference

dbAMP DRAMP CAFPdb Antifungipept

Other

Active against E. coli IID-861 (MIC 2 uM), S. enteritidis, K. pneumoniae JCM1662T (MIC 3 uM), P. vulgaris, Y. enterocolitica, P. aeruginosa IFO-3446 (MIC 3 uM), C. jejuni, S. aureus
Evidence 14 Activity

Activity

MIC
Antifungal
3 µM

Target

Pseudomonas aeruginosa IFO-3446

Source & Reference

dbAMP DRAMP CAFPdb Antifungipept

Other

2 uM), S. enteritidis, K. pneumoniae JCM1662T (MIC 3 uM), P. vulgaris, Y. enterocolitica, P. aeruginosa IFO-3446 (MIC 3 uM), C. jejuni, S. aureus JCM-2151 or MRSA (MIC 2 uM), S. mutans, C. diphtheriae, L. monocyto
Evidence 15 Activity

Activity

MIC
Antifungal
2 µM

Target

Staphylococcus aureus JCM-2151 or MRSA

Source & Reference

dbAMP DRAMP CAFPdb Antifungipept

Other

(MIC 3 uM), P. vulgaris, Y. enterocolitica, P. aeruginosa IFO-3446 (MIC 3 uM), C. jejuni, S. aureus JCM-2151 or MRSA (MIC 2 uM), S. mutans, C. diphtheriae, L. monocytogenes IDF-1 b(MIC 0.3 uM) and Clostridium perfring
Evidence 16 Activity

Activity

MIC
Antifungal
0.3 µM

Target

L. monocytogenes IDF-1 b

Source & Reference

dbAMP DRAMP CAFPdb Antifungipept

Other

(MIC 3 uM), C. jejuni, S. aureus JCM-2151 or MRSA (MIC 2 uM), S. mutans, C. diphtheriae, L. monocytogenes IDF-1 b(MIC 0.3 uM) and Clostridium perfringens. Also active against E. coli ATCC 2592, M. luteus, and S. cer
Evidence 17 Activity

Activity

MIC
Antiparasitic
2 µM

Target

Escherichia coli IID-861

Source & Reference

dbAMP 3.0 DRAMP

Other

Source Activity Label Source Definition
Antiparasitic
DRAMP antiparasitic activity supported by activity records against parasitic targets. dbAMP 3.0 classified under the Anti-parasitic function class.
Active against E. coli IID-861 (MIC 2 uM), S. enteritidis, K. pneumoniae JCM1662T (MIC 3 uM), P. vulgaris, Y. enterocolitica, P. ae
Evidence 18 Activity

Activity

MIC
Antiparasitic
3 µM

Target

Klebsiella pneumoniae JCM1662T

Source & Reference

dbAMP 3.0 DRAMP

Other

Active against E. coli IID-861 (MIC 2 uM), S. enteritidis, K. pneumoniae JCM1662T (MIC 3 uM), P. vulgaris, Y. enterocolitica, P. aeruginosa IFO-3446 (MIC 3 uM), C. jejuni, S. aureus
Evidence 19 Activity

Activity

MIC
Antiparasitic
3 µM

Target

Pseudomonas aeruginosa IFO-3446

Source & Reference

dbAMP 3.0 DRAMP

Other

2 uM), S. enteritidis, K. pneumoniae JCM1662T (MIC 3 uM), P. vulgaris, Y. enterocolitica, P. aeruginosa IFO-3446 (MIC 3 uM), C. jejuni, S. aureus JCM-2151 or MRSA (MIC 2 uM), S. mutans, C. diphtheriae, L. monocyto
Evidence 20 Activity

Activity

MIC
Antiparasitic
2 µM

Target

Staphylococcus aureus JCM-2151 or MRSA

Source & Reference

dbAMP 3.0 DRAMP

Other

(MIC 3 uM), P. vulgaris, Y. enterocolitica, P. aeruginosa IFO-3446 (MIC 3 uM), C. jejuni, S. aureus JCM-2151 or MRSA (MIC 2 uM), S. mutans, C. diphtheriae, L. monocytogenes IDF-1 b(MIC 0.3 uM) and Clostridium perfring
Evidence 21 Activity

Activity

MIC
Antiparasitic
0.3 µM

Target

L. monocytogenes IDF-1 b

Source & Reference

dbAMP 3.0 DRAMP

Other

(MIC 3 uM), C. jejuni, S. aureus JCM-2151 or MRSA (MIC 2 uM), S. mutans, C. diphtheriae, L. monocytogenes IDF-1 b(MIC 0.3 uM) and Clostridium perfringens. Also active against E. coli ATCC 2592, M. luteus, and S. cer
Evidence 22 Activity

Activity

MIC
Antiviral
2 µM

Target

Escherichia coli IID-861

Source & Reference

dbAMP 3.0 DRAMP

Other

Source Activity Label Source Definition
Antiviral
DRAMP antiviral activity supported by activity records against viral targets. dbAMP 3.0 classified under the Anti-viral function class.
Active against E. coli IID-861 (MIC 2 uM), S. enteritidis, K. pneumoniae JCM1662T (MIC 3 uM), P. vulgaris, Y. enterocolitica, P. ae
Evidence 23 Activity

Activity

MIC
Antiviral
3 µM

Target

Klebsiella pneumoniae JCM1662T

Source & Reference

dbAMP 3.0 DRAMP

Other

Active against E. coli IID-861 (MIC 2 uM), S. enteritidis, K. pneumoniae JCM1662T (MIC 3 uM), P. vulgaris, Y. enterocolitica, P. aeruginosa IFO-3446 (MIC 3 uM), C. jejuni, S. aureus
Evidence 24 Activity

Activity

MIC
Antiviral
3 µM

Target

Pseudomonas aeruginosa IFO-3446

Source & Reference

dbAMP 3.0 DRAMP

Other

2 uM), S. enteritidis, K. pneumoniae JCM1662T (MIC 3 uM), P. vulgaris, Y. enterocolitica, P. aeruginosa IFO-3446 (MIC 3 uM), C. jejuni, S. aureus JCM-2151 or MRSA (MIC 2 uM), S. mutans, C. diphtheriae, L. monocyto
Evidence 25 Activity

Activity

MIC
Antiviral
2 µM

Target

Staphylococcus aureus JCM-2151 or MRSA

Source & Reference

dbAMP 3.0 DRAMP

Other

(MIC 3 uM), P. vulgaris, Y. enterocolitica, P. aeruginosa IFO-3446 (MIC 3 uM), C. jejuni, S. aureus JCM-2151 or MRSA (MIC 2 uM), S. mutans, C. diphtheriae, L. monocytogenes IDF-1 b(MIC 0.3 uM) and Clostridium perfring
Evidence 26 Activity

Activity

MIC
Antiviral
0.3 µM

Target

L. monocytogenes IDF-1 b

Source & Reference

dbAMP 3.0 DRAMP

Other

(MIC 3 uM), C. jejuni, S. aureus JCM-2151 or MRSA (MIC 2 uM), S. mutans, C. diphtheriae, L. monocytogenes IDF-1 b(MIC 0.3 uM) and Clostridium perfringens. Also active against E. coli ATCC 2592, M. luteus, and S. cer
Evidence 27 Activity

Activity

Cell-penetrating Peptides

Target

A549 cells

Source & Reference

CPPsite3.0
CPPsite3

Other

Source Activity Label Source Definition
Cell-penetrating Peptides
CPPsite3.0 experimentally validated cell-penetrating peptide annotation.
Natural residues Linear L Cationic
Protein derived
Alexa647 dye
Lysosomes
Endocytosis
In vitro
CCCCCCCCSSCCC
Evidence 28 Activity

Activity

5% Hemolysis
Toxicity
256 µg/mL
Hemolytic Cytotoxic
5% Hemolysis | 256 | µg/mL | target cell: Human erythrocytes

Target

Human erythrocytes

Source & Reference

NA
DBAASP
Comparative antimicrobial activity and mechanism of action of bovine lactoferricin-derived synthetic peptides. | Biometals | 2011
Evidence 29 Activity

Activity

5% Hemolysis
Toxicity
64 µg/mL
Hemolytic Cytotoxic
5% Hemolysis | 64 | µg/mL | target cell: Human erythrocytes

Target

Human erythrocytes

Source & Reference

NA
DBAASP
Comparative antimicrobial activity and mechanism of action of bovine lactoferricin-derived synthetic peptides. | Biometals | 2011
Additional Detail Fields 1 fields
CCCCCCCCSSCCC