Back to search

Peptide record

TPDB10650

Brevinin-2R Brevinin-2R (B2R) Antibacterial Anticancer Antifungal Antiparasitic Cell-penetrating Peptides Toxicity standard
25 amino acids
Basic Information
3D PDB MODEL
Drag to rotate. Click a residue or atom to inspect it; the selected residue is highlighted in amber.
TPDB10650
Brevinin-2R Brevinin-2R (B2R)
Antibacterial Anticancer Antifungal Antiparasitic Cell-penetrating Peptides Toxicity
Anti-infective peptides Cancer-related peptides Toxicity and safety peptides Delivery and barrier-penetrating peptides
AntiBP3 Peptipedia ACP740 _ACP240 AntiCP 2.0 CancerPPD 2.0 SATPdb dbACP iACP-DRLF pep-lab Antifungipept CAFPdb dbAMP dbAMP 3.0 CPPsite3.0 ToxinPred 3.0
standard
No
A 25-aa standard natural multi-activity (Antibacterial, Anticancer, Antifungal, and other sources) peptide sequence curated from AntiBP3, Peptipedia, ACP740 _ACP240, and other sources, with an available 3D structural model.
Sequence
KLKNFAKGVAQSLLNKASCKLSGQC
Physicochemical Analysis
C114H198N34O33S2
RDEHIMPTWY
K
2637.15
10.39
5
0
9
+5
3.07
-0.164
86.00
Mammalian: 1.3 hour Yeast: 3 min E.coli: 2 min
125
4.74
9
Residue Composition
number
3
A
0
R
2
N
0
D
2
C
0
E
2
Q
2
G
0
H
0
I
4
L
5
K
0
M
1
F
0
P
3
S
0
T
0
W
0
Y
1
V
Amino Acid Distribution
A: 3 R: 0 N: 2 D: 0 C: 2 E: 0 Q: 2 G: 2 H: 0 I: 0 L: 4 K: 5 M: 0 F: 1 P: 0 S: 3 T: 0 W: 0 Y: 0 V: 1
Chemical Descriptors
25
C114H198N34O33S2
2637.15
10.39
+5
3.07
-0.164
Free
L
Cationic
Linear
Cyclic
Free
Antimicrobial
Rana ridibunda
Evidence Records 8 records
Evidence 1 Activity

Activity

Antibacterial

Source & Reference

dbAMP

Other

Source Activity Label Source Definition
Antibacterial
dbAMP classified as Antibacterial under the Anti-bacterial function class.
Active against S. aureus, M. luteus, Bacillus spKR-8104, E. coli, S. typhimurium, P. aeruginosa, K. pneumonidae and fungi, such as C. albicans and C. tropicalis.
Evidence 2 Activity

Activity

IC50
IC50 = 131.5 µg/ml
Anticancer
131.5 µg/mL

Target

Cervical Cancer
HeLa
Cervix

Assay & Model

MTT assay
24-h

Source & Reference

dbAMP 3.0 CancerPPD 2.0 dbACP SATPdb
CancerPPD2
2022

Other

Source Activity Label Source Definition
Anticancer
CancerPPD 2.0 manually curated experimentally verified anticancer peptide/protein annotation. dbAMP 3.0 classified as Anticancer under the disease-associated peptide function classes.
Evidence 3 Activity

Activity

Antifungal

Source & Reference

dbAMP CAFPdb Antifungipept

Other

Source Activity Label Source Definition
Antifungal
dbAMP classified under the Anti-fungal function class.
Active against S. aureus, M. luteus, Bacillus spKR-8104, E. coli, S. typhimurium, P. aeruginosa, K. pneumonidae and fungi, such as C. albicans and C. tropicalis.
Evidence 4 Activity

Activity

Antiparasitic

Source & Reference

dbAMP 3.0

Other

Source Activity Label Source Definition
Antiparasitic
dbAMP 3.0 classified under the Anti-parasitic function class.
Active against S. aureus, M. luteus, Bacillus spKR-8104, E. coli, S. typhimurium, P. aeruginosa, K. pneumonidae and fungi, such as C. albicans and C. tropicalis.
Evidence 5 Activity

Activity

Cell-penetrating Peptides

Target

HeLa Cells

Source & Reference

CPPsite3.0
CPPsite3

Other

Source Activity Label Source Definition
Cell-penetrating Peptides
CPPsite3.0 experimentally validated cell-penetrating peptide annotation.
Non-natural residues Cyclic L Cationic
Protein derived
GFP and β-galactosidase
Cytoplasm
Transfection rate in B2R/GFP complex is more than fourfolds greater than B2R/β-gal
Energy- and temperature-dependent pathways (endocytosis pathways)
In vitro
Evidence 6 Activity

Activity

0% Hemolysis
Toxicity
500 µg/mL
Hemolytic Cytotoxic
0% Hemolysis | 500 | µg/mL | target cell: Human erythrocytes

Target

Human erythrocytes

Source & Reference

NA
DBAASP
Anti-leishmanial activity of Brevinin 2R and its Lauric acid conjugate type against L. major: In vitro mechanism of actions and in vivo treatment potentials. | PLoS Negl Trop Dis | 2019
Evidence 7 Activity

Activity

50% Hemolysis
Toxicity
250 µg/mL
Hemolytic Cytotoxic
50% Hemolysis | 250 | µg/mL | target cell: Human erythrocytes

Target

Human erythrocytes

Source & Reference

NA
DBAASP
Anti-leishmanial activity of Brevinin 2R and its Lauric acid conjugate type against L. major: In vitro mechanism of actions and in vivo treatment potentials. | PLoS Negl Trop Dis | 2019
Evidence 8 Activity

Activity

Toxicity
Hemolytic Cytotoxic
Hemolytic_activity: Not available | Cytotoxicity: Not available

Target

erythrocytes

Source & Reference

NA
DRAMP
J Cell Mol Med. 2008 Jun 12(3):1005-22.