TPDB

3D PDB MODEL

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TPDB ID TPDB10531

Activity

Antibacterial Anticancer Antifungal Antiparasitic Toxicity

Activity_category_statistics

Anti-infective peptides Cancer-related peptides Toxicity and safety peptides

Database_Source

AntiBP3 dbAMP DRAMP pep-lab Peptipedia AFP-GFuse Antifungipept CAFPdb

Sequence_Type

standard

Has_Modification No

Description A 25-aa standard natural multi-activity (Antibacterial, Anticancer, Antifungal, and other sources) peptide sequence curated from AntiBP3, dbAMP, DRAMP, and other sources, with an available 3D structural model.

Sequence

GWASKIGQTLGKIAKVGLKELIQPK

FASTA

>TPDB10531|Antibacterial;Anticancer;Antifungal;Antiparasitic;Toxicity|length=25
GWASKIGQTLGKIAKVGLKELIQPK

Formula C123H211N33O32

Absent Amino Acids RNDCHMFY

Common Amino Acids K

Mass 2664.23

PI 10.71

Basic Residues 5

Acidic Residues 1

Hydrophobic Residues 10

Net Charge +4

Boman Index 4.16

Hydrophobicity -0.116

Aliphatic Index 113.20

Half Life

Mammalian: 30 hour Yeast: >20 hour E.coli: >10 hour

Extinction Coefficient Cystines 5500

Absorbance 280nm 206.44

Polar Residues 4

number

2

A

0

R

0

N

0

D

0

C

1

E

2

Q

4

G

0

H

3

I

3

L

5

K

0

M

0

F

1

P

1

S

1

T

1

W

0

Y

1

V

Amino Acid Distribution

A: 2 R: 0 N: 0 D: 0 C: 0 E: 1 Q: 2 G: 4 H: 0 I: 3 L: 3 K: 5 M: 0 F: 0 P: 1 S: 1 T: 1 W: 1 Y: 0 V: 1

Length

25

Formula

C123H211N33O32

Mass

2664.23

PI

10.71

Net Charge

+4

Boman Index

4.16

Hydrophobicity

-0.116

Evidence 1 Activity

Activity

Detail Source Activity Antibacterial

Source & Reference

Database Source dbAMP DRAMP

Other

Source Activity Label	Source Definition
Antibacterial	dbAMP classified as Antibacterial under the Anti-bacterial function class.

Evidence Text

Activity against E. coli (25922), S. pyogenes (19615) (MIC 10-50ug/ml) and P. aeruginosa (27883 (MIC 50-100ug/ml). Also active against protozoan P. caudatum (minimal disruptive conc MDC 10 ug/ml), A. castellani (MDC 2 ug/ml), and T. pyriformis (MDC 20 ug/ml).

Evidence 2 Activity

Activity

Detail Source Activity Antifungal

Source & Reference

Database Source dbAMP DRAMP CAFPdb Antifungipept

Other

Source Activity Label	Source Definition
Antifungal	dbAMP classified under the Anti-fungal function class.

Evidence Text

Activity against E. coli (25922), S. pyogenes (19615) (MIC 10-50ug/ml) and P. aeruginosa (27883 (MIC 50-100ug/ml). Also active against protozoan P. caudatum (minimal disruptive conc MDC 10 ug/ml), A. castellani (MDC 2 ug/ml), and T. pyriformis (MDC 20 ug/ml).

Evidence 3 Activity

Activity

Detail Source Activity Antiparasitic

Source & Reference

Database Source DRAMP

Other

Evidence Text

Activity against E. coli (25922), S. pyogenes (19615) (MIC 10-50ug/ml) and P. aeruginosa (27883 (MIC 50-100ug/ml). Also active against protozoan P. caudatum (minimal disruptive conc MDC 10 ug/ml), A. castellani (MDC 2 ug/ml), and T. pyriformis (MDC 20 ug/ml).

Evidence 4 Activity

Activity

Detail Source Activity Toxicity

Toxicity Type Hemolytic

Toxicity Raw

NA | target cell: Human erythrocytes

Target

Target Cell Human erythrocytes

Source & Reference

Database Source NA

Source Database DBAASP

Reference

QSAR Reveals Decreased Lipophilicity of Polar Residues Determines the Selectivity of Antimicrobial Peptide Activity. | ACS Omega | 2024

Evidence 5 Activity

Activity

Activity Endpoint 25% Cytotoxicity

Detail Source Activity Toxicity

Activity Measurement 256 µg/mL

Toxicity Type Cytotoxic

Toxicity Raw

25% Cytotoxicity | 256 | µg/mL | target cell: Human keratinocytes HaCat

Target

Target Cell Human keratinocytes HaCat

Source & Reference

Database Source NA

Source Database DBAASP

Reference

QSAR Reveals Decreased Lipophilicity of Polar Residues Determines the Selectivity of Antimicrobial Peptide Activity. | ACS Omega | 2024