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Peptide record

TPDB10465

Lycosin-I Antibacterial Anticancer Antifungal Antiparasitic Cell-penetrating Peptides Hemolysis Toxicity standard
24 amino acids
Basic Information
3D PDB MODEL
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TPDB10465
Lycosin-I
Antibacterial Anticancer Antifungal Antiparasitic Cell-penetrating Peptides Hemolysis Toxicity
Anti-infective peptides Cancer-related peptides Toxicity and safety peptides Delivery and barrier-penetrating peptides
AntiBP3 Peptipedia AntiCP 2.0 SATPdb dbACP iACP-DRLF AFP-GFuse Antifungipept CAFPdb dbAMP i2APP CellPPD-MOD CPPsite3.0 APD_complete APD_hemolytic HemoPI Hemolytic2 PD_Hemolysis ToxinPred 3.0
standard
No
A 24-aa standard natural multi-activity (Antibacterial, Anticancer, Antifungal, and other sources) peptide sequence curated from AntiBP3, Peptipedia, AntiCP 2.0, and other sources, with an available 3D structural model.
Sequence
RKGWFKAMKSIAKFIAKEKLKEHL
Physicochemical Analysis
C136H223N37O30S1
NDCQPTYV
K
2888.56
10.91
9
2
11
+6
6.29
-0.608
77.50
Mammalian: 1 hour Yeast: 2 min E.coli: 2 min
5500
190.41
1
Residue Composition
number
3
A
1
R
0
N
0
D
0
C
2
E
0
Q
1
G
1
H
2
I
2
L
7
K
1
M
2
F
0
P
1
S
0
T
1
W
0
Y
0
V
Amino Acid Distribution
A: 3 R: 1 N: 0 D: 0 C: 0 E: 2 Q: 0 G: 1 H: 1 I: 2 L: 2 K: 7 M: 1 F: 2 P: 0 S: 1 T: 0 W: 1 Y: 0 V: 0
Chemical Descriptors
24
C136H223N37O30S1
2888.56
10.91
+6
6.29
-0.608
Free
L
Cationic and amphiphilic
Linear
Free
Evidence Records 9 records
Evidence 1 Activity

Activity

MIC
Antibacterial
12.5 µM

Target

B. thuringiensis Israel H14

Source & Reference

dbAMP

Other

Source Activity Label Source Definition
Antibacterial
dbAMP classified as Antibacterial under the Anti-bacterial function class.
nless specified, Gram-Positive E. faecalis CGMCC1.100 (MIC 25 uM), B. subtilis CGMCC1.88, B. thuringiensis Israel H14 (MIC 12.5 uM), B. cereus CMCC1.126 (MIC 6.2 uM), S. aureus AB94004, C. glutamicum CGMCC1.818, S. epider
Evidence 2 Activity

Activity

MIC
Antifungal
12.5 µM

Target

B. thuringiensis Israel H14

Source & Reference

dbAMP CAFPdb Antifungipept

Other

Source Activity Label Source Definition
Antifungal
dbAMP classified under the Anti-fungal function class.
nless specified, Gram-Positive E. faecalis CGMCC1.100 (MIC 25 uM), B. subtilis CGMCC1.88, B. thuringiensis Israel H14 (MIC 12.5 uM), B. cereus CMCC1.126 (MIC 6.2 uM), S. aureus AB94004, C. glutamicum CGMCC1.818, S. epider
Evidence 3 Activity

Activity

MIC
Antiparasitic
12.5 µM

Target

B. thuringiensis Israel H14

Source & Reference

NA

Other

nless specified, Gram-Positive E. faecalis CGMCC1.100 (MIC 25 uM), B. subtilis CGMCC1.88, B. thuringiensis Israel H14 (MIC 12.5 uM), B. cereus CMCC1.126 (MIC 6.2 uM), S. aureus AB94004, C. glutamicum CGMCC1.818, S. epider
Evidence 4 Activity

Activity

Cell-penetrating Peptides

Target

HeLa , Mat-Lylu Cells , Sw480, Hek293T, HUVEC And Hbl100 Cell Lines

Assay & Model

Healthy female 4 to 6 week-old BALB/c mice
Healthy female 4 to 6 week-old BALB/c mice

Source & Reference

CPPsite3.0
CPPsite3

Other

Source Activity Label Source Definition
Cell-penetrating Peptides
CPPsite3.0 experimentally validated cell-penetrating peptide annotation.
Non-natural residues Linear L Cationic and amphiphilic
Protein derived
Gold nanoparticles (NRs)
Cytoplasm
Lycosin-I and TAT showed comparable intracellular internalization with an increase at least 26 times than without CPP.
Clathrin and caveolae mediated endocytosis
In vitro and in vivo
Evidence 5 Activity

Activity

10% Hemolysis
Toxicity
40 µM
Hemolytic Cytotoxic
10% Hemolysis | 40 | µM | target cell: Human erythrocytes

Target

Human erythrocytes

Source & Reference

NA
DBAASP
A novel spider peptide toxin suppresses tumor growth through dual signaling pathways. | Curr Mol Med | 2012
Evidence 6 Activity

Activity

37% Hemolysis
Toxicity
200 µM
Hemolytic Cytotoxic
37% Hemolysis | 200 | µM | target cell: Human erythrocytes

Target

Human erythrocytes

Source & Reference

NA
DBAASP
Antimicrobial potential of lycosin-I, a cationic and amphiphilic peptide from the venom of the spider Lycosa singorensis. | Curr Mol Med | 2013
Evidence 7 Activity

Activity

25% Killing
Toxicity
40 µM
Cytotoxic
25% Killing | 40 | µM | target cell: Human hepatocyte cells HL-7702 (L02)

Target

Human hepatocyte cells HL-7702 (L02)

Source & Reference

NA
DBAASP
A novel spider peptide toxin suppresses tumor growth through dual signaling pathways. | Curr Mol Med | 2012
Evidence 8 Activity

Activity

20% Killing
Toxicity
40 µM
Cytotoxic
20% Killing | 40 | µM | target cell: Mouse skin epidermal cells JB6

Target

Mouse skin epidermal cells JB6

Source & Reference

NA
DBAASP
A novel spider peptide toxin suppresses tumor growth through dual signaling pathways. | Curr Mol Med | 2012
Evidence 9 Hemolysis

Source & Reference

APD_complete APD_hemolytic HemoPI PD_Hemolysis
APD_complete APD_hemolytic PD_Hemolysis

Other

Source Activity Label Source Definition
Hemolysis
APD_complete APD-derived hemolytic peptide annotation. APD_hemolytic APD hemolytic peptide annotation.