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Peptide record

TPDB10398

Antibacterial Antifungal Hemolysis Toxicity standard

24 amino acids

3D PDB MODEL

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TPDB ID TPDB10398

Activity

Antibacterial Antifungal Hemolysis Toxicity

Activity_category_statistics

Anti-infective peptides Toxicity and safety peptides

Database_Source

AntiBP3 dbAMP Peptipedia Antifungipept CAFPdb APD_complete APD_hemolytic Hemolytic2 PD_Hemolysis ToxinPred 3.0

Sequence_Type

standard

Has_Modification No

Description A 24-aa standard-residue multi-activity (Antibacterial, Antifungal, Hemolysis, and other sources) peptide sequence curated from AntiBP3, dbAMP, Peptipedia, and other sources, with an available 3D structural model.

Sequence

FLSTLLKVAFKVVPTLFCPITKKC

FASTA

>TPDB10398|Antibacterial;Antifungal;Hemolysis;Toxicity|length=24
FLSTLLKVAFKVVPTLFCPITKKC

Sequence-only estimates for a linear peptide with free termini, not experimentally measured molecular properties. Mass and formula exclude cyclization and disulfide formation. The optical estimates assume paired cysteines, 280 nm, 1 g/L and a 1 cm path. The N-end rule estimate is not a measured plasma half-life.

Formula C130H214N28O29S2

Absent Amino Acids RNDEQGHMWY

Common Amino Acids LK

Mass 2697.42

PI 10.17

Basic Residues 4

Acidic Residues 0

Hydrophobic Residues 12

Net Charge +4

Boman Index -0.70

Hydrophobicity 1.075

Aliphatic Index 121.67

N-end Rule Half-life Estimate

Mammalian: 1.1 hour Yeast: 3 min E.coli: 2 min

Extinction Coefficient Cystines 125

Absorbance 280nm 0.05

Polar Residues 6

number

Amino Acid Distribution

A: 1 R: 0 N: 0 D: 0 C: 2 E: 0 Q: 0 G: 0 H: 0 I: 1 L: 4 K: 4 M: 0 F: 3 P: 2 S: 1 T: 3 W: 0 Y: 0 V: 3

Length

24 reviewed residue count

Formula

C130H214N28O29S2 sequence-only estimate (linear, free termini)

Mass

2697.42 sequence-only estimate (linear, free termini)

10.17 sequence-only estimate (linear, free termini)

Net Charge

+4 sequence-only estimate (linear, free termini)

Boman Index

-0.70 sequence-only estimate (linear, free termini)

Hydrophobicity

1.075 sequence-only estimate (linear, free termini)

Evidence 1 Activity

Activity

Activity Endpoint MIC

Detail Source Activity Antibacterial

Activity Measurement 18.75 µg/mL

Target

Target Organism Escherichia coli ATCC 25922

Source & Reference

Database Source dbAMP

Other

Source Activity Label	Source Definition
Antibacterial	dbAMP classified as Antibacterial under the Anti-bacterial function class.

Source Activity Label	Source Definition
Antifungal	dbAMP classified under the Anti-fungal function class.

Evidence Text

Active against Gram- E. coli ATCC 25922 (MIC 18.75 ug/ml), B. dysenteriae (MIC 9.38 ug/ml), Gram+ S. aureus ATCC 2592 (MIC 1.17 ug/ml), B. subtilis

Evidence 7 Activity

Activity

Activity Endpoint MIC

Detail Source Activity Antifungal

Activity Measurement 9.38 µg/mL

Target

Target Organism B. dysenteriae

Source & Reference

Database Source dbAMP CAFPdb Antifungipept

Other

Evidence Text

Active against Gram- E. coli ATCC 25922 (MIC 18.75 ug/ml), B. dysenteriae (MIC 9.38 ug/ml), Gram+ S. aureus ATCC 2592 (MIC 1.17 ug/ml), B. subtilis ATCC 6633 (MIC 4.7 ug/ml), and f

Evidence 8 Activity

Activity

Activity Endpoint MIC

Detail Source Activity Antifungal

Activity Measurement 1.17 µg/mL

Target

Target Organism Staphylococcus aureus ATCC 2592

Source & Reference

Database Source dbAMP CAFPdb Antifungipept

Other

Evidence Text

Evidence 9 Activity

Activity

Activity Endpoint MIC

Detail Source Activity Antifungal

Activity Measurement 4.7 µg/mL

Target

Target Organism B. subtilis ATCC 6633

Source & Reference

Database Source dbAMP CAFPdb Antifungipept

Other

Evidence Text

8.75 ug/ml), B. dysenteriae (MIC 9.38 ug/ml), Gram+ S. aureus ATCC 2592 (MIC 1.17 ug/ml), B. subtilis ATCC 6633 (MIC 4.7 ug/ml), and fungi C. albicans ATCC 2002 (MIC 4.7 ug/ml).

Evidence 10 Activity

Activity

Activity Endpoint MIC

Detail Source Activity Antifungal

Activity Measurement 4.7 µg/mL

Target

Target Organism Candida albicans ATCC 2002

Source & Reference

Database Source dbAMP CAFPdb Antifungipept

Other

Evidence Text

m+ S. aureus ATCC 2592 (MIC 1.17 ug/ml), B. subtilis ATCC 6633 (MIC 4.7 ug/ml), and fungi C. albicans ATCC 2002 (MIC 4.7 ug/ml).

Evidence 11 Activity

Activity

Activity Endpoint 8% Hemolysis

Detail Source Activity Toxicity

Activity Measurement 25 µg/mL

Toxicity Type Hemolytic Cytotoxic

Toxicity Raw

8% Hemolysis | 25 | µg/mL | target cell: Rabbit erythrocytes

Target

Target Cell Rabbit erythrocytes

Source & Reference

Database Source NA

Source Database DBAASP

Reference

Antimicrobial peptide diversity in the skin of the torrent frog, Amolops jingdongensis. | Amino Acids | 2013

Evidence 12 Activity

Activity

Activity Endpoint 25% Hemolysis

Detail Source Activity Toxicity

Activity Measurement 50 µg/mL

Toxicity Type Hemolytic Cytotoxic

Toxicity Raw

25% Hemolysis | 50 | µg/mL | target cell: Rabbit erythrocytes

Target

Target Cell Rabbit erythrocytes

Source & Reference

Database Source NA

Source Database DBAASP

Reference

Antimicrobial peptide diversity in the skin of the torrent frog, Amolops jingdongensis. | Amino Acids | 2013

Evidence 13 Activity

Activity

Activity Endpoint 83% Hemolysis

Detail Source Activity Toxicity

Activity Measurement 100 µg/mL

Toxicity Type Hemolytic Cytotoxic

Toxicity Raw

83% Hemolysis | 100 | µg/mL | target cell: Rabbit erythrocytes

Target

Target Cell Rabbit erythrocytes

Source & Reference

Database Source NA

Source Database DBAASP

Reference

Antimicrobial peptide diversity in the skin of the torrent frog, Amolops jingdongensis. | Amino Acids | 2013

Evidence 14 Hemolysis

Source & Reference

source APD_complete APD_hemolytic PD_Hemolysis

Database Source APD_complete APD_hemolytic PD_Hemolysis

Other

Source Activity Label	Source Definition
Hemolysis	APD_complete APD-derived hemolytic peptide annotation. APD_hemolytic APD hemolytic peptide annotation.