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Peptide record

TPDB10259

SMP24 Antibacterial Anticancer Antifungal Hemolysis Toxicity standard

24 amino acids

3D PDB MODEL

Drag to rotate. Click a residue or atom to inspect it; the selected residue is highlighted in amber.

TPDB ID TPDB10259

Peptide_Name

SMP24

Activity

Antibacterial Anticancer Antifungal Hemolysis Toxicity

Activity_category_statistics

Anti-infective peptides Cancer-related peptides Toxicity and safety peptides

Database_Source

AntiBP3 dbAMP DRAMP Peptipedia CancerPPD 2.0 dbACP AFP-GFuse Antifungipept CAFPdb APD_complete PD_Hemolysis ToxinPred 3.0

Sequence_Type

standard

Has_Modification No

Description A 24-aa standard natural multi-activity (Antibacterial, Anticancer, Antifungal, and other sources) peptide sequence curated from AntiBP3, dbAMP, DRAMP, and other sources, with an available 3D structural model.

Sequence

IWSFLIKAATKLLPSLFGGGKKDS

FASTA

>TPDB10259|Antibacterial;Anticancer;Antifungal;Hemolysis;Toxicity|length=24
IWSFLIKAATKLLPSLFGGGKKDS

Formula C123H197N29O31

Absent Amino Acids RNCEQHMYV

Common Amino Acids LK

Mass 2578.09

PI 10.55

Basic Residues 4

Acidic Residues 1

Hydrophobic Residues 11

Net Charge +3

Boman Index -1.11

Hydrophobicity 0.313

Aliphatic Index 105.83

Half Life

Mammalian: 20 hour Yeast: 30 min E.coli: >10 hour

Extinction Coefficient Cystines 5500

Absorbance 280nm 213.34

Polar Residues 4

number

Amino Acid Distribution

A: 2 R: 0 N: 0 D: 1 C: 0 E: 0 Q: 0 G: 3 H: 0 I: 2 L: 4 K: 4 M: 0 F: 2 P: 1 S: 3 T: 1 W: 1 Y: 0 V: 0

Length

Formula

C123H197N29O31

Mass

2578.09

10.55

Net Charge

Boman Index

-1.11

Hydrophobicity

0.313

C_terminal_Modification

Free

Chirality

Linear_or_Cyclic

Linear

N_terminal_Modification

Free

Nature

Antimicrobial

Origin

Scorpio maurus palmatus Egyptian scorpion Scorpio maurus palmatus

Evidence 1 Activity

Activity

Activity Endpoint MIC

Detail Source Activity Antibacterial

Activity Measurement 4-8 µg/mL

Target

Target Organism Staphylococcus aureus SH100

Source & Reference

Database Source dbAMP DRAMP

Other

Source Activity Label	Source Definition
Antibacterial	dbAMP classified as Antibacterial under the Anti-bacterial function class. DRAMP antibacterial activity supported by MIC records against bacterial target organisms.

Evidence Text

Active against B. subtilis NCIMB 8024, S. epidermidis sp., S. aureus SH100 (MIC 4-8 ug/ml), E. coli JM109 (MIC 64 ug/ml), and C. albicans (MIC 32 ug/ml). Structure: The peptide can

Evidence 2 Activity

Activity

Activity Endpoint MIC

Detail Source Activity Antibacterial

Activity Measurement 64 µg/mL

Target

Target Organism Escherichia coli JM109

Source & Reference

Database Source dbAMP DRAMP

Other

Evidence Text

tive against B. subtilis NCIMB 8024, S. epidermidis sp., S. aureus SH100 (MIC 4-8 ug/ml), E. coli JM109 (MIC 64 ug/ml), and C. albicans (MIC 32 ug/ml). Structure: The peptide can adopt a helical conformation

Evidence 3 Activity

Activity

Activity Endpoint MIC

Detail Source Activity Antibacterial

Activity Measurement 32 µg/mL

Target

Target Organism Candida albicans

Source & Reference

Database Source dbAMP DRAMP

Other

Evidence Text

4, S. epidermidis sp., S. aureus SH100 (MIC 4-8 ug/ml), E. coli JM109 (MIC 64 ug/ml), and C. albicans (MIC 32 ug/ml). Structure: The peptide can adopt a helical conformation in 60% TFE, laying a basis for m

Evidence 4 Activity

Activity

Activity Endpoint LC50

Activity Raw

LC50 = 16 ± 0.9 µg/mL

Detail Source Activity Anticancer

Activity Measurement 16

Target

Cancer Type Leukemia Cancer

Cell Line CCRF-CEM

Tissue Affected Blood

Assay & Model

Assay CellTiter-Glo assay

Testing Time 24-h

Source & Reference

Database Source CancerPPD 2.0 dbACP

Source CancerPPD2

Year 2019

Other

Source Activity Label	Source Definition
Anticancer	CancerPPD 2.0 manually curated experimentally verified anticancer peptide/protein annotation.

Evidence 5 Activity

Activity

Activity Endpoint LC50

Activity Raw

LC50 = 16 ± 1.3 µg/mL

Detail Source Activity Anticancer

Activity Measurement 16

Target

Cancer Type Leukemia Cancer

Cell Line KG-1a

Tissue Affected Blood

Assay & Model

Assay CellTiter-Glo assay

Testing Time 24-h

Source & Reference

Database Source CancerPPD 2.0 dbACP

Source CancerPPD2

Year 2019

Evidence 6 Activity

Activity

Activity Raw

Fig 2

Detail Source Activity Anticancer

Target

Cancer Type Leukemia Cancer

Cell Line CCRF-CEM

Tissue Affected Blood

Assay & Model

Assay LDH leakage assay

Source & Reference

Database Source CancerPPD 2.0 dbACP

Source CancerPPD2

Year 2019

Evidence 7 Activity

Activity

Activity Raw

Fig 2

Detail Source Activity Anticancer

Target

Cancer Type Leukemia Cancer

Cell Line KG-1a

Tissue Affected Blood

Assay & Model

Assay LDH leakage assay

Source & Reference

Database Source CancerPPD 2.0 dbACP

Source CancerPPD2

Year 2019

Evidence 8 Activity

Activity

Activity Endpoint IC50

Activity Raw

IC50 = 4.06 ± 0.60 µM

Detail Source Activity Anticancer

Activity Measurement 4.06

Target

Cancer Type Lung Cancer

Cell Line A-549

Tissue Affected Lung

Assay & Model

Assay MTT assay

Testing Time 24-h

Source & Reference

Database Source CancerPPD 2.0 dbACP

Source CancerPPD2

Year 2022

Evidence 9 Activity

Activity

Activity Endpoint IC50

Activity Raw

IC50 = 4.64 ± 0.18 µM

Detail Source Activity Anticancer

Activity Measurement 4.64

Target

Cancer Type Lung Cancer

Cell Line H-3122

Tissue Affected Lung

Assay & Model

Assay MTT assay

Testing Time 24-h

Source & Reference

Database Source CancerPPD 2.0 dbACP

Source CancerPPD2

Year 2022

Evidence 10 Activity

Activity

Activity Endpoint IC50

Activity Raw

IC50 = 6.34 ± 0.53 µM

Detail Source Activity Anticancer

Activity Measurement 6.34

Target

Cancer Type Lung Cancer

Cell Line PC-9

Tissue Affected Lung

Assay & Model

Assay MTT assay

Testing Time 24-h

Source & Reference

Database Source CancerPPD 2.0 dbACP

Source CancerPPD2

Year 2022

Evidence 11 Activity

Activity

Activity Endpoint IC50

Activity Raw

IC50 = 7.07 ± 0.81 µM

Detail Source Activity Anticancer

Activity Measurement 7.07

Target

Cancer Type Lung Cancer

Cell Line H-460

Tissue Affected Lung

Assay & Model

Assay MTT assay

Testing Time 24-h

Source & Reference

Database Source CancerPPD 2.0 dbACP

Source CancerPPD2

Year 2022

Evidence 12 Activity

Activity

Activity Endpoint IC50

Activity Raw

IC50 = 5.524 µM

Detail Source Activity Anticancer

Activity Measurement 5.524 µM

Target

Cancer Type Liver Cancer

Cell Line HepG-2

Tissue Affected Liver

Assay & Model

Assay MTT assay

Testing Time 24-h

Source & Reference

Database Source CancerPPD 2.0 dbACP

Source CancerPPD2

Year 2022

Evidence 13 Activity

Activity

Activity Endpoint MIC

Detail Source Activity Antifungal

Activity Measurement 4-8 µg/mL

Target

Target Organism Staphylococcus aureus SH100

Source & Reference

Database Source dbAMP DRAMP CAFPdb Antifungipept

Other

Source Activity Label	Source Definition
Antifungal	dbAMP classified under the Anti-fungal function class. DRAMP antifungal activity supported by activity records against fungal target organisms.

Evidence Text

Active against B. subtilis NCIMB 8024, S. epidermidis sp., S. aureus SH100 (MIC 4-8 ug/ml), E. coli JM109 (MIC 64 ug/ml), and C. albicans (MIC 32 ug/ml). Structure: The peptide can

Evidence 14 Activity

Activity

Activity Endpoint MIC

Detail Source Activity Antifungal

Activity Measurement 64 µg/mL

Target

Target Organism Escherichia coli JM109

Source & Reference

Database Source dbAMP DRAMP CAFPdb Antifungipept

Other

Evidence Text

Evidence 15 Activity

Activity

Activity Endpoint MIC

Detail Source Activity Antifungal

Activity Measurement 32 µg/mL

Target

Target Organism Candida albicans

Source & Reference

Database Source dbAMP DRAMP CAFPdb Antifungipept

Other

Evidence Text

Evidence 16 Activity

Activity

Activity Endpoint 5% Hemolysis

Detail Source Activity Toxicity

Activity Measurement 32 µg/mL

Toxicity Type Hemolytic Cytotoxic

Toxicity Raw

5% Hemolysis | 32 | µg/mL | target cell: Sheep erythrocytes

Target

Target Cell Sheep erythrocytes

Source & Reference

Database Source NA

Source Database DBAASP

Reference

Characterisation of three alpha-helical antimicrobial peptides from the venom of Scorpio maurus palmatus. | Toxicon | 2016

Evidence 17 Activity

Activity

Activity Endpoint 15% Hemolysis

Detail Source Activity Toxicity

Activity Measurement 64 µg/mL

Toxicity Type Hemolytic Cytotoxic

Toxicity Raw

15% Hemolysis | 64 | µg/mL | target cell: Sheep erythrocytes

Target

Target Cell Sheep erythrocytes

Source & Reference

Database Source NA

Source Database DBAASP

Reference

Characterisation of three alpha-helical antimicrobial peptides from the venom of Scorpio maurus palmatus. | Toxicon | 2016

Evidence 18 Activity

Activity

Activity Endpoint 52% Hemolysis

Detail Source Activity Toxicity

Activity Measurement 128 µg/mL

Toxicity Type Hemolytic Cytotoxic

Toxicity Raw

52% Hemolysis | 128 | µg/mL | target cell: Sheep erythrocytes

Target

Target Cell Sheep erythrocytes

Source & Reference

Database Source NA

Source Database DBAASP

Reference

Characterisation of three alpha-helical antimicrobial peptides from the venom of Scorpio maurus palmatus. | Toxicon | 2016

Evidence 19 Activity

Activity

Activity Endpoint 90% Hemolysis

Detail Source Activity Toxicity

Activity Measurement 512 µg/mL

Toxicity Type Hemolytic Cytotoxic

Toxicity Raw

90% Hemolysis | 512 | µg/mL | target cell: Sheep erythrocytes

Target

Target Cell Sheep erythrocytes

Source & Reference

Database Source NA

Source Database DBAASP

Reference

Characterisation of three alpha-helical antimicrobial peptides from the venom of Scorpio maurus palmatus. | Toxicon | 2016

Evidence 20 Hemolysis

Source & Reference

source APD_complete PD_Hemolysis

Database Source APD_complete PD_Hemolysis

Other

Source Activity Label	Source Definition
Hemolysis	APD_complete APD-derived hemolytic peptide annotation.