TPDB

3D PDB MODEL

Drag to rotate. Click a residue or atom to inspect it; the selected residue is highlighted in amber.

TPDB ID TPDB10225

Activity

Antibacterial Anticancer Antifungal Antiparasitic Hemolysis Toxicity

Activity_category_statistics

Anti-infective peptides Cancer-related peptides Toxicity and safety peptides

Database_Source

AntiBP3 dbAMP DRAMP Peptipedia Antifungipept CAFPdb HemoPI Hemolytic2 PD_Hemolysis ToxinPred 3.0

Sequence_Type

standard

Has_Modification No

Description A 23-aa standard natural multi-activity (Antibacterial, Anticancer, Antifungal, and other sources) peptide sequence curated from AntiBP3, dbAMP, DRAMP, and other sources, with an available 3D structural model.

Sequence

GLFGKSSVWGRKYYVDLAGCAKA

FASTA

>TPDB10225|Antibacterial;Anticancer;Antifungal;Antiparasitic;Hemolysis;Toxicity|length=23
GLFGKSSVWGRKYYVDLAGCAKA

Formula C114H174N30O30S1

Absent Amino Acids NEQHIMPT

Common Amino Acids G

Mass 2476.88

PI 9.90

Basic Residues 4

Acidic Residues 1

Hydrophobic Residues 11

Net Charge +3

Boman Index -1.28

Hydrophobicity 0.013

Aliphatic Index 72.17

Half Life

Mammalian: 30 hour Yeast: >20 hour E.coli: >10 hour

Extinction Coefficient Cystines 8480

Absorbance 280nm 342.37

Polar Residues 5

number

3

A

1

R

0

N

1

D

1

C

0

E

0

Q

4

G

0

H

0

I

2

L

3

K

0

M

1

F

0

P

2

S

0

T

1

W

2

Y

2

V

Amino Acid Distribution

A: 3 R: 1 N: 0 D: 1 C: 1 E: 0 Q: 0 G: 4 H: 0 I: 0 L: 2 K: 3 M: 0 F: 1 P: 0 S: 2 T: 0 W: 1 Y: 2 V: 2

Length

23

Formula

C114H174N30O30S1

Mass

2476.88

PI

9.90

Net Charge

+3

Boman Index

-1.28

Hydrophobicity

0.013

Evidence 1 Activity

Activity

Activity Endpoint % Hemolysis

Detail Source Activity Hemolysis

Activity Measurement 2.2 %

Hemolysis Raw

[Ref.17272268] RCH=17.6 ± 2.2% against rabbit red blood cells. RCH, red cell hemolysis.

Target

RBC Target rabbit red blood cells

Source & Reference

Database Source Hemolytik PD_Hemolysis

Source DRAMP4

Reference

Mol Cell Proteomics. 2007 May 6(5):882-894.

Other

Source Activity Label	Source Definition
Hemolysis	Hemolytik experimentally validated hemolytic peptide annotation.

Evidence 2 Activity

Activity

Activity Endpoint 18% Hemolysis

Detail Source Activity Toxicity

Activity Measurement 50 µg/mL

Toxicity Type Hemolytic Cytotoxic

Toxicity Raw

18% Hemolysis | 50 | µg/mL | target cell: Human erythrocytes

Target

Target Cell Human erythrocytes

Source & Reference

Database Source NA

Source Database DBAASP

Reference

Anti-infection peptidomics of amphibian skin. | Mol Cell Proteomics | 2007

Evidence 3 Activity

Activity

Activity Endpoint (9.2±0.7)% degranulation

Detail Source Activity Toxicity

Activity Measurement 50 µg/mL

Toxicity Type Cytotoxic

Toxicity Raw

(9.2±0.7)% degranulation | 50 | µg/mL | target cell: Human mast cells

Target

Target Cell Human mast cells

Source & Reference

Database Source NA

Source Database DBAASP

Reference

Anti-infection peptidomics of amphibian skin. | Mol Cell Proteomics | 2007

Evidence 4 Activity

Activity

Detail Source Activity Toxicity

Toxicity Type Hemolytic

Toxicity Raw

Hemolytic_activity: [Ref.17272268] RCH=17.6 ± 2.2% against rabbit red blood cells. RCH, red cell hemolysis.

Target

Target Cell erythrocytes

Source & Reference

Database Source NA

Source Database DRAMP

Reference

Mol Cell Proteomics. 2007 May 6(5):882-894.

Evidence 5 Hemolysis

Activity

Activity Measurement 2.2 %

Activity Endpoint % Hemolysis

Hemolysis Raw

[Ref.17272268] RCH=17.6 ± 2.2% against rabbit red blood cells. RCH, red cell hemolysis.

Detail Source Activity Hemolysis

Target

RBC Target rabbit red blood cells

Source & Reference

source HemoPI PD_Hemolysis

Reference

Mol Cell Proteomics. 2007 May 6(5):882-894.

Source DRAMP4

Database Source PD_Hemolysis