TPDB

3D PDB MODEL

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TPDB ID TPDB10072

Activity

Antibacterial Anticancer Antifungal Toxicity

Activity_category_statistics

Anti-infective peptides Cancer-related peptides Toxicity and safety peptides

Database_Source

AntiBP3 dbAMP DRAMP Peptipedia CancerPPD 2.0 Antifungipept CAFPdb

Sequence_Type

standard

Has_Modification No

Description A 23-aa standard natural multi-activity (Antibacterial, Anticancer, Antifungal, and other sources) peptide sequence curated from AntiBP3, dbAMP, DRAMP, and other sources, with an available 3D structural model.

Sequence

FLPIVAKLLSGLLGRKKRRQRRR

FASTA

>TPDB10072|Antibacterial;Anticancer;Antifungal;Toxicity|length=23
FLPIVAKLLSGLLGRKKRRQRRR

Formula C124H225N45O26

Absent Amino Acids NDCEHMTWY

Common Amino Acids R

Mass 2762.44

PI 13.10

Basic Residues 9

Acidic Residues 0

Hydrophobic Residues 9

Net Charge +9

Boman Index 4.67

Hydrophobicity -0.570

Aliphatic Index 118.70

Half Life

Mammalian: 1.1 hour Yeast: 3 min E.coli: 2 min

Extinction Coefficient Cystines 0

Absorbance 280nm 0.00

Polar Residues 2

number

1

A

6

R

0

N

0

D

0

C

0

E

1

Q

2

G

0

H

1

I

5

L

3

K

0

M

1

F

1

P

1

S

0

T

0

W

0

Y

1

V

Amino Acid Distribution

A: 1 R: 6 N: 0 D: 0 C: 0 E: 0 Q: 1 G: 2 H: 0 I: 1 L: 5 K: 3 M: 0 F: 1 P: 1 S: 1 T: 0 W: 0 Y: 0 V: 1

Length

23

Formula

C124H225N45O26

Mass

2762.44

PI

13.10

Net Charge

+9

Boman Index

4.67

Hydrophobicity

-0.570

Evidence 1 Activity

Activity

Activity Endpoint IC50

Detail Source Activity Toxicity

Activity Measurement 9.976 µM

Toxicity Type Cytotoxic

Toxicity Raw

IC50 | 9.976 | µM | target cell: Human microvascular endothelial cells HMEC-1

Target

Target Cell Human microvascular endothelial cells HMEC-1

Source & Reference

Database Source NA

Source Database DBAASP

Reference

Identification and target-modifications of temporin-PE: A novel antimicrobial peptide in the defensive skin secretions of the edible frog, Pelophylax kl. esculentus. | Biochem Biophys Res Commun | 2017

Evidence 2 Activity

Activity

Activity Endpoint 50% Hemolysis

Detail Source Activity Toxicity

Activity Measurement 44.65 µM

Toxicity Type Hemolytic Cytotoxic

Toxicity Raw

50% Hemolysis | 44.65 | µM | target cell: Horse erythrocytes

Target

Target Cell Horse erythrocytes

Source & Reference

Database Source NA

Source Database DBAASP

Reference

Identification and target-modifications of temporin-PE: A novel antimicrobial peptide in the defensive skin secretions of the edible frog, Pelophylax kl. esculentus. | Biochem Biophys Res Commun | 2017

Evidence 3 Activity

Activity

Activity Endpoint HC50

Detail Source Activity Toxicity

Activity Measurement 44.64 µM

Toxicity Type Hemolytic

Toxicity Raw

Hemolytic_activity: [Ref.29191658]HC50=44.64 µM against horse red blood cells

Target

Target Cell erythrocytes

Source & Reference

Database Source NA

Source Database DRAMP

Reference

Biochem Biophys Res Commun. 2018 Jan 22 495(4):2539-2546.