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Peptide record

TPDB09826

Epinecidin-1 Anti-inflammatory Antibacterial Anticancer Antifungal Antiparasitic Antiviral Toxicity standard

21 amino acids

3D PDB MODEL

Drag to rotate. Click a residue or atom to inspect it; the selected residue is highlighted in amber.

TPDB ID TPDB09826

Peptide_Name

Epinecidin-1

Activity

Anti-inflammatory Antibacterial Anticancer Antifungal Antiparasitic Antiviral Toxicity

Activity_category_statistics

Anti-infective peptides Cancer-related peptides Immunity and inflammation peptides Toxicity and safety peptides

Database_Source

Peptipedia AntiBP3 dbAMP DRAMP AntiCP 2.0 CancerPPD 2.0 SATPdb dbACP iACP-DRLF Antifungipept CAFPdb dbAMP 3.0 AI4AVP DRAVP 2.0

Sequence_Type

standard

Has_Modification No

Description A 21-aa standard natural multi-activity (Anti-inflammatory, Antibacterial, Anticancer, and other sources) peptide sequence curated from Peptipedia, AntiBP3, dbAMP, and other sources, with an available 3D structural model.

Sequence

GFIFHIIKGLFHAGKMIHGLV

FASTA

>TPDB09826|Anti-inflammatory;Antibacterial;Anticancer;Antifungal;Antiparasitic;Antiviral;Toxicity|length=21
GFIFHIIKGLFHAGKMIHGLV

Formula C114H175N29O22S1

Absent Amino Acids RNDCEQPSTWY

Common Amino Acids GI

Mass 2335.88

PI 10.60

Basic Residues 5

Acidic Residues 0

Hydrophobic Residues 12

Net Charge +2

Boman Index -1.18

Hydrophobicity 1.090

Aliphatic Index 130.00

Half Life

Mammalian: 30 hour Yeast: >20 hour E.coli: >10 hour

Extinction Coefficient Cystines 0

Absorbance 280nm 0.00

Polar Residues 0

number

Amino Acid Distribution

A: 1 R: 0 N: 0 D: 0 C: 0 E: 0 Q: 0 G: 4 H: 3 I: 4 L: 2 K: 2 M: 1 F: 3 P: 0 S: 0 T: 0 W: 0 Y: 0 V: 1

Length

Formula

C114H175N29O22S1

Mass

2335.88

10.60

Net Charge

Boman Index

-1.18

Hydrophobicity

1.090

C_terminal_Modification

Free

Chirality

Linear_or_Cyclic

Linear

N_terminal_Modification

Free

Nature

Antimicrobial

Origin

Epinephelus nebulosus

Evidence 1 Activity

Activity

Detail Source Activity Antibacterial

Source & Reference

Database Source dbAMP DRAMP

Other

Source Activity Label	Source Definition
Antibacterial	dbAMP classified as Antibacterial under the Anti-bacterial function class.

Evidence Text

Antiviral activity against foot-and-mouth disease virus (FMDV) in vitro (EC50 0.6 ug/ml). It also showed antiviral activity against non-enveloped, spherical virus (betanodavirus) (Wang Y et al. 2010 Peptides 31: 1026-33).

Evidence 2 Activity

Activity

Activity Raw

45% Cytotoxicity at 50 mg/L

Detail Source Activity Anticancer

Target

Cancer Type Cervical Cancer

Cell Line HeLa

Tissue Affected Cervix

Assay & Model

Assay MTT/MTS assay

Testing Time 3-h

Source & Reference

Database Source dbAMP 3.0 CancerPPD 2.0 dbACP SATPdb

Source CancerPPD2

Year 2013

Other

Source Activity Label	Source Definition
Anticancer	CancerPPD 2.0 manually curated experimentally verified anticancer peptide/protein annotation. dbAMP 3.0 classified as Anticancer under the disease-associated peptide function classes.

Evidence 3 Activity

Activity

Activity Raw

38% Cytotoxicity at 25 mg/L

Detail Source Activity Anticancer

Target

Cancer Type Cervical Cancer

Cell Line HeLa

Tissue Affected Cervix

Assay & Model

Assay MTT/MTS assay

Testing Time 3-h

Source & Reference

Database Source dbAMP 3.0 CancerPPD 2.0 dbACP SATPdb

Source CancerPPD2

Year 2013

Evidence 4 Activity

Activity

Activity Raw

75% Cytotoxicity at 50 mg/L

Detail Source Activity Anticancer

Target

Cancer Type Cervical Cancer

Cell Line HeLa

Tissue Affected Cervix

Assay & Model

Assay MTT/MTS assay

Testing Time 6-h

Source & Reference

Database Source dbAMP 3.0 CancerPPD 2.0 dbACP SATPdb

Source CancerPPD2

Year 2013

Evidence 5 Activity

Activity

Activity Raw

65% Cytotoxicity at 25 mg/L

Detail Source Activity Anticancer

Target

Cancer Type Cervical Cancer

Cell Line HeLa

Tissue Affected Cervix

Assay & Model

Assay MTT/MTS assay

Testing Time 6-h

Source & Reference

Database Source dbAMP 3.0 CancerPPD 2.0 dbACP SATPdb

Source CancerPPD2

Year 2013

Evidence 6 Activity

Activity

Activity Raw

75 % Cytotoxicity at 50 mg/L

Detail Source Activity Anticancer

Target

Cancer Type Cervical Cancer

Cell Line HeLa

Tissue Affected Cervix

Assay & Model

Assay MTT/MTS assay

Testing Time 12-h

Source & Reference

Database Source dbAMP 3.0 CancerPPD 2.0 dbACP SATPdb

Source CancerPPD2

Year 2013

Evidence 7 Activity

Activity

Activity Raw

65% Cytotoxicity at 25 mg/L

Detail Source Activity Anticancer

Target

Cancer Type Cervical Cancer

Cell Line HeLa

Tissue Affected Cervix

Assay & Model

Assay MTT/MTS assay

Testing Time 12-h

Source & Reference

Database Source dbAMP 3.0 CancerPPD 2.0 dbACP SATPdb

Source CancerPPD2

Year 2013

Evidence 8 Activity

Activity

Activity Raw

83% Cytotoxicity at 50 mg/L

Detail Source Activity Anticancer

Target

Cancer Type Cervical Cancer

Cell Line HeLa

Tissue Affected Cervix

Assay & Model

Assay MTT/MTS assay

Testing Time 24-h

Source & Reference

Database Source dbAMP 3.0 CancerPPD 2.0 dbACP SATPdb

Source CancerPPD2

Year 2013

Evidence 9 Activity

Activity

Activity Raw

77 % Cytotoxicity at 25 mg/L

Detail Source Activity Anticancer

Target

Cancer Type Cervical Cancer

Cell Line HeLa

Tissue Affected Cervix

Assay & Model

Assay MTT/MTS assay

Testing Time 24-h

Source & Reference

Database Source dbAMP 3.0 CancerPPD 2.0 dbACP SATPdb

Source CancerPPD2

Year 2013

Evidence 10 Activity

Activity

Activity Raw

82 % Cytotoxicity at 50 mg/L

Detail Source Activity Anticancer

Target

Cancer Type Fibrosarcoma

Cell Line HT-1080

Tissue Affected Fibrous connective tissue

Assay & Model

Assay MTT/MTS assay

Testing Time 3-h

Source & Reference

Database Source dbAMP 3.0 CancerPPD 2.0 dbACP SATPdb

Source CancerPPD2

Year 2013

Evidence 11 Activity

Activity

Activity Raw

85 % Cytotoxicity at 25 mg/L

Detail Source Activity Anticancer

Target

Cancer Type Fibrosarcoma

Cell Line HT-1080

Tissue Affected Fibrous connective tissue

Assay & Model

Assay MTT/MTS assay

Testing Time 3-h

Source & Reference

Database Source dbAMP 3.0 CancerPPD 2.0 dbACP SATPdb

Source CancerPPD2

Year 2013

Evidence 12 Activity

Activity

Activity Raw

42 % Cytotoxicity at 12.5 mg/L

Detail Source Activity Anticancer

Target

Cancer Type Fibrosarcoma

Cell Line HT-1080

Tissue Affected Fibrous connective tissue

Assay & Model

Assay MTT/MTS assay

Testing Time 3-h

Source & Reference

Database Source dbAMP 3.0 CancerPPD 2.0 dbACP SATPdb

Source CancerPPD2

Year 2013

Evidence 13 Activity

Activity

Activity Raw

41 % Cytotoxicity at 6.25 mg/L

Detail Source Activity Anticancer

Target

Cancer Type Fibrosarcoma

Cell Line HT-1080

Tissue Affected Fibrous connective tissue

Assay & Model

Assay MTT/MTS assay

Testing Time 3-h

Source & Reference

Database Source dbAMP 3.0 CancerPPD 2.0 dbACP SATPdb

Source CancerPPD2

Year 2013

Evidence 14 Activity

Activity

Activity Raw

39 % Cytotoxicity at 3.125 mg/L

Detail Source Activity Anticancer

Target

Cancer Type Fibrosarcoma

Cell Line HT-1080

Tissue Affected Fibrous connective tissue

Assay & Model

Assay MTT/MTS assay

Testing Time 3-h

Source & Reference

Database Source dbAMP 3.0 CancerPPD 2.0 dbACP SATPdb

Source CancerPPD2

Year 2013

Evidence 15 Activity

Activity

Activity Raw

90 % Cytotoxicity at 50 mg/L

Detail Source Activity Anticancer

Target

Cancer Type Fibrosarcoma

Cell Line HT-1080

Tissue Affected Fibrous connective tissue

Assay & Model

Assay MTT/MTS assay

Testing Time 6-h

Source & Reference

Database Source dbAMP 3.0 CancerPPD 2.0 dbACP SATPdb

Source CancerPPD2

Year 2013

Evidence 16 Activity

Activity

Activity Raw

95 % Cytotoxicity at 25 mg/L

Detail Source Activity Anticancer

Target

Cancer Type Fibrosarcoma

Cell Line HT-1080

Tissue Affected Fibrous connective tissue

Assay & Model

Assay MTT/MTS assay

Testing Time 6-h

Source & Reference

Database Source dbAMP 3.0 CancerPPD 2.0 dbACP SATPdb

Source CancerPPD2

Year 2013

Evidence 17 Activity

Activity

Activity Raw

45 % Cytotoxicity at 12.5 mg/L

Detail Source Activity Anticancer

Target

Cancer Type Fibrosarcoma

Cell Line HT-1080

Tissue Affected Fibrous connective tissue

Assay & Model

Assay MTT/MTS assay

Testing Time 6-h

Source & Reference

Database Source dbAMP 3.0 CancerPPD 2.0 dbACP SATPdb

Source CancerPPD2

Year 2013

Evidence 18 Activity

Activity

Activity Raw

25 % Cytotoxicity at 6.25 mg/L

Detail Source Activity Anticancer

Target

Cancer Type Fibrosarcoma

Cell Line HT-1080

Tissue Affected Fibrous connective tissue

Assay & Model

Assay MTT/MTS assay

Testing Time 6-h

Source & Reference

Database Source dbAMP 3.0 CancerPPD 2.0 dbACP SATPdb

Source CancerPPD2

Year 2013

Evidence 19 Activity

Activity

Activity Raw

25 % Cytotoxicity at 3.125 mg/L

Detail Source Activity Anticancer

Target

Cancer Type Fibrosarcoma

Cell Line HT-1080

Tissue Affected Fibrous connective tissue

Assay & Model

Assay MTT/MTS assay

Testing Time 6-h

Source & Reference

Database Source dbAMP 3.0 CancerPPD 2.0 dbACP SATPdb

Source CancerPPD2

Year 2013

Evidence 20 Activity

Activity

Activity Raw

95 % Cytotoxicity at 50 mg/L

Detail Source Activity Anticancer

Target

Cancer Type Fibrosarcoma

Cell Line HT-1080

Tissue Affected Fibrous connective tissue

Assay & Model

Assay MTT/MTS assay

Testing Time 12-h

Source & Reference

Database Source dbAMP 3.0 CancerPPD 2.0 dbACP SATPdb

Source CancerPPD2

Year 2013

Evidence 21 Activity

Activity

Activity Raw

96 % Cytotoxicity at 25 mg/L

Detail Source Activity Anticancer

Target

Cancer Type Fibrosarcoma

Cell Line HT-1080

Tissue Affected Fibrous connective tissue

Assay & Model

Assay MTT/MTS assay

Testing Time 12-h

Source & Reference

Database Source dbAMP 3.0 CancerPPD 2.0 dbACP SATPdb

Source CancerPPD2

Year 2013

Evidence 22 Activity

Activity

Activity Raw

46 % Cytotoxicity at 12.5 mg/L

Detail Source Activity Anticancer

Target

Cancer Type Fibrosarcoma

Cell Line HT-1080

Tissue Affected Fibrous connective tissue

Assay & Model

Assay MTT/MTS assay

Testing Time 12-h

Source & Reference

Database Source dbAMP 3.0 CancerPPD 2.0 dbACP SATPdb

Source CancerPPD2

Year 2013

Evidence 23 Activity

Activity

Activity Raw

30 % Cytotoxicity at 6.25 mg/L

Detail Source Activity Anticancer

Target

Cancer Type Fibrosarcoma

Cell Line HT-1080

Tissue Affected Fibrous connective tissue

Assay & Model

Assay MTT/MTS assay

Testing Time 12-h

Source & Reference

Database Source dbAMP 3.0 CancerPPD 2.0 dbACP SATPdb

Source CancerPPD2

Year 2013

Evidence 24 Activity

Activity

Activity Raw

28 % Cytotoxicity at 3.125 mg/L

Detail Source Activity Anticancer

Target

Cancer Type Fibrosarcoma

Cell Line HT-1080

Tissue Affected Fibrous connective tissue

Assay & Model

Assay MTT/MTS assay

Testing Time 12-h

Source & Reference

Database Source dbAMP 3.0 CancerPPD 2.0 dbACP SATPdb

Source CancerPPD2

Year 2013

Evidence 25 Activity

Activity

Activity Raw

84 % Cytotoxicity at 50 mg/L

Detail Source Activity Anticancer

Target

Cancer Type Fibrosarcoma

Cell Line HT-1080

Tissue Affected Fibrous connective tissue

Assay & Model

Assay MTT/MTS assay

Testing Time 24-h

Source & Reference

Database Source dbAMP 3.0 CancerPPD 2.0 dbACP SATPdb

Source CancerPPD2

Year 2013

Evidence 26 Activity

Activity

Activity Raw

85 % Cytotoxicity at 25 mg/L

Detail Source Activity Anticancer

Target

Cancer Type Fibrosarcoma

Cell Line HT-1080

Tissue Affected Fibrous connective tissue

Assay & Model

Assay MTT/MTS assay

Testing Time 24-h

Source & Reference

Database Source dbAMP 3.0 CancerPPD 2.0 dbACP SATPdb

Source CancerPPD2

Year 2013

Evidence 27 Activity

Activity

Activity Raw

38 % Cytotoxicity at 12.5 mg/L

Detail Source Activity Anticancer

Target

Cancer Type Fibrosarcoma

Cell Line HT-1080

Tissue Affected Fibrous connective tissue

Assay & Model

Assay MTT/MTS assay

Testing Time 24-h

Source & Reference

Database Source dbAMP 3.0 CancerPPD 2.0 dbACP SATPdb

Source CancerPPD2

Year 2013

Evidence 28 Activity

Activity

Activity Raw

30 % Cytotoxicity at 6.25 mg/L

Detail Source Activity Anticancer

Target

Cancer Type Fibrosarcoma

Cell Line HT-1080

Tissue Affected Fibrous connective tissue

Assay & Model

Assay MTT/MTS assay

Testing Time 24-h

Source & Reference

Database Source dbAMP 3.0 CancerPPD 2.0 dbACP SATPdb

Source CancerPPD2

Year 2013

Evidence 29 Activity

Activity

Activity Raw

28 % Cytotoxicity at 3.125 mg/L

Detail Source Activity Anticancer

Target

Cancer Type Fibrosarcoma

Cell Line HT-1080

Tissue Affected Fibrous connective tissue

Assay & Model

Assay MTT/MTS assay

Testing Time 24-h

Source & Reference

Database Source dbAMP 3.0 CancerPPD 2.0 dbACP SATPdb

Source CancerPPD2

Year 2013

Evidence 30 Activity

Activity

Detail Source Activity Antifungal

Source & Reference

Database Source dbAMP DRAMP CAFPdb Antifungipept

Other

Source Activity Label	Source Definition
Antifungal	dbAMP classified under the Anti-fungal function class.

Evidence Text

Evidence 31 Activity

Activity

Detail Source Activity Antiparasitic

Source & Reference

Database Source dbAMP 3.0 DRAMP

Other

Source Activity Label	Source Definition
Antiparasitic	dbAMP 3.0 classified under the Anti-parasitic function class.

Evidence Text

Evidence 32 Activity

Activity

Detail Source Activity Antiviral

Source & Reference

Database Source dbAMP 3.0 DRAMP DRAVP 2.0

Other

Source Activity Label	Source Definition
Antiviral	dbAMP 3.0 classified under the Anti-viral function class.

Evidence Text

Evidence 33 Activity

Activity

Activity Endpoint 50% Cytotoxicity

Detail Source Activity Toxicity

Activity Measurement 19.5±3.1 µg/mL

Toxicity Type Cytotoxic

Toxicity Raw

50% Cytotoxicity | 19.5±3.1 | µg/mL | target cell: Baby Hamster Kidney cells BHK-21

Target

Target Cell Baby Hamster Kidney cells BHK-21

Source & Reference

Database Source NA

Source Database DBAASP

Reference

Grouper (Epinephelus coioides) antimicrobial peptide epinecidin-1 exhibits antiviral activity against foot-and-mouth disease virus in vitro. | Peptides | 2018

Evidence 34 Activity

Activity

Activity Endpoint 50% Hemolysis

Detail Source Activity Toxicity

Activity Measurement >200 µg/mL

Toxicity Type Hemolytic Cytotoxic

Toxicity Raw

50% Hemolysis | >200 | µg/mL | target cell: Sheep erythrocytes

Target

Target Cell Sheep erythrocytes

Source & Reference

Database Source NA

Source Database DBAASP

Reference

Truncated antimicrobial peptides from marine organisms retain anticancer activity and antibacterial activity against multidrug-resistant Staphylococcus aureus. | Peptides | 2013

Evidence 35 Activity

Activity

Activity Endpoint % Hemolysis

Detail Source Activity Toxicity

Activity Measurement 0 %

Toxicity Type Hemolytic

Toxicity Raw

Hemolytic_activity: [Ref.23598079] 0% hemolysis at 25 mg/L , 10% hemolysis at 50 mg/L , 15% hemolysis at 100 mg/L , 30% hemolysis at 200 mg/L , 50% hemolysis at 400 mg/L against sheep red blood cells

Target

Target Cell erythrocytes

Source & Reference

Database Source NA

Source Database DRAMP

Reference

Peptides. 2013 Jun 44:139-48. doi: 10.1016/j.peptides.2013.04.004.