TPDB

3D PDB MODEL

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TPDB ID TPDB09584

Peptide_Name

ppTG20

Activity

Antibacterial Cell-penetrating Peptides Toxicity

Activity_category_statistics

Anti-infective peptides Toxicity and safety peptides Delivery and barrier-penetrating peptides

Database_Source

AntiBP3 dbAMP DRAMP Peptipedia CellPPD CPPsite3.0 MLCPP 2.0 PerseuCPP

Sequence_Type

standard

Has_Modification No

Description A 20-aa standard natural multi-activity (Antibacterial, Cell-penetrating Peptides, and Toxicity) peptide sequence curated from AntiBP3, dbAMP, DRAMP, and other sources, with an available 3D structural model.

Sequence

GLFRALLRLLRSLWRLLLRA

FASTA

>TPDB09584|Antibacterial;Cell-penetrating Peptides;Toxicity|length=20
GLFRALLRLLRSLWRLLLRA

Formula C115H198N36O22

Absent Amino Acids NDCEQHIKMPTYV

Common Amino Acids L

Mass 2437.06

PI 13.00

Basic Residues 5

Acidic Residues 0

Hydrophobic Residues 13

Net Charge +5

Boman Index -4.17

Hydrophobicity 0.800

Aliphatic Index 185.50

Half Life

Mammalian: 30 hour Yeast: >20 hour E.coli: >10 hour

Extinction Coefficient Cystines 5500

Absorbance 280nm 225.68

Polar Residues 1

number

2

A

5

R

0

N

0

D

0

C

0

E

0

Q

1

G

0

H

0

I

9

L

0

K

0

M

1

F

0

P

1

S

0

T

1

W

0

Y

0

V

Amino Acid Distribution

A: 2 R: 5 N: 0 D: 0 C: 0 E: 0 Q: 0 G: 1 H: 0 I: 0 L: 9 K: 0 M: 0 F: 1 P: 0 S: 1 T: 0 W: 1 Y: 0 V: 0

Length

20

Formula

C115H198N36O22

Mass

2437.06

PI

13.00

Net Charge

+5

Boman Index

-4.17

Hydrophobicity

0.800

Chirality

L

Linearity

Linear

SMILES

NCC(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](Cc1ccccc1)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CO)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC1=CN=C(C)/C/1=C\C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](C)C(=O)O

Evidence 1 Activity

Activity

Detail Source Activity Cell-penetrating Peptides

Target

Cell Line HeLa

Source & Reference

Database Source CPPsite3.0

Source CPPsite3

Other

Source Activity Label	Source Definition
Cell-penetrating Peptides	CPPsite3.0 experimentally validated cell-penetrating peptide annotation.

CPP Category Natural residues Linear L

Source of Peptide Synthetic

Cargo Type Nucleic acid

In Vitro or In Vivo In vitro

DSSP Secondary Structure CTHHHHHHHHHHHHHHTSCC

Evidence 2 Activity

Activity

Activity Endpoint 10% Hemolysis

Detail Source Activity Toxicity

Activity Measurement 240 µM

Toxicity Type Hemolytic Cytotoxic

Toxicity Raw

10% Hemolysis | 240 | µM | target cell: Mouse erythrocytes

Target

Target Cell Mouse erythrocytes

Source & Reference

Database Source NA

Source Database DBAASP

Reference

Antibacterial activity and dual mechanisms of peptide analog derived from cell-penetrating peptide against Salmonella typhimurium and Streptococcus pyogenes. | Appl Microbiol Biotechnol | 2013

DSSP_Secondary_Structure

CTHHHHHHHHHHHHHHTSCC