TPDB

3D PDB MODEL

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TPDB ID TPDB09035

Activity

Antibacterial Hemolysis Toxicity

Activity_category_statistics

Anti-infective peptides Toxicity and safety peptides

Database_Source

AntiBP3 dbAMP DRAMP Peptipedia HemoPI Hemolytic2 PD_Hemolysis

Sequence_Type

standard

Has_Modification No

Description A 19-aa standard natural multi-activity (Antibacterial, Hemolysis, and Toxicity) peptide sequence curated from AntiBP3, dbAMP, DRAMP, and other sources, with an available 3D structural model.

Sequence

IGKKWKRIVKRIKKFLRKL

FASTA

>TPDB09035|Antibacterial;Hemolysis;Toxicity|length=19
IGKKWKRIVKRIKKFLRKL

Formula C117H208N36O20

Absent Amino Acids ANDCEQHMPSTY

Common Amino Acids K

Mass 2439.17

PI 12.73

Basic Residues 10

Acidic Residues 0

Hydrophobic Residues 8

Net Charge +10

Boman Index 4.41

Hydrophobicity -0.737

Aliphatic Index 117.89

Half Life

Mammalian: 20 hour Yeast: 30 min E.coli: >10 hour

Extinction Coefficient Cystines 5500

Absorbance 280nm 225.49

Polar Residues 0

number

0

A

3

R

0

N

0

D

0

C

0

E

0

Q

1

G

0

H

3

I

2

L

7

K

0

M

1

F

0

P

0

S

0

T

1

W

0

Y

1

V

Amino Acid Distribution

A: 0 R: 3 N: 0 D: 0 C: 0 E: 0 Q: 0 G: 1 H: 0 I: 3 L: 2 K: 7 M: 0 F: 1 P: 0 S: 0 T: 0 W: 1 Y: 0 V: 1

Length

19

Formula

C117H208N36O20

Mass

2439.17

PI

12.73

Net Charge

+10

Boman Index

4.41

Hydrophobicity

-0.737

Evidence 1 Activity

Activity

Activity Endpoint MHC10

Detail Source Activity Hemolysis

Activity Measurement 9.2 µM

Hemolysis Raw

[Ref.22521196] MHC10=9.2 µM against human red blood cells

Target

RBC Target human red blood cells

Source & Reference

Database Source Hemolytik PD_Hemolysis

Source DRAMP4

Reference

Peptides. 2012 Jun 35(2):239-247.

Other

Source Activity Label	Source Definition
Hemolysis	Hemolytik experimentally validated hemolytic peptide annotation.

Evidence 2 Activity

Activity

Activity Endpoint 10% Hemolysis

Detail Source Activity Toxicity

Activity Measurement 9.2 µM

Toxicity Type Hemolytic Cytotoxic

Toxicity Raw

10% Hemolysis | 9.2 | µM | target cell: Human erythrocytes

Target

Target Cell Human erythrocytes

Source & Reference

Database Source NA

Source Database DBAASP

Reference

Prokaryotic selectivity and LPS-neutralizing activity of short antimicrobial peptides designed from the human antimicrobial peptide LL-37 | Peptides | 2012

Evidence 3 Activity

Activity

Detail Source Activity Toxicity

Toxicity Type Hemolytic

Toxicity Raw

Hemolytic_activity: [Ref.22521196] MHC10=9.2 µM against human red blood cells

Target

Target Cell erythrocytes

Source & Reference

Database Source NA

Source Database DRAMP

Reference

Peptides. 2012 Jun 35(2):239-247.

Evidence 4 Hemolysis

Activity

Activity Measurement 9.2 µM

Activity Endpoint MHC10

Hemolysis Raw

[Ref.22521196] MHC10=9.2 µM against human red blood cells

Detail Source Activity Hemolysis

Target

RBC Target human red blood cells

Source & Reference

source HemoPI PD_Hemolysis

Reference

Peptides. 2012 Jun 35(2):239-247.

Source DRAMP4

Database Source PD_Hemolysis