TPDB

3D PDB MODEL

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TPDB ID TPDB08725

Activity

Antibacterial Anticancer Hemolysis Toxicity

Activity_category_statistics

Anti-infective peptides Cancer-related peptides Toxicity and safety peptides

Database_Source

AntiBP3 Peptipedia HemoPI Hemolytic2 PD_Hemolysis

Sequence_Type

standard

Has_Modification No

Description A 18-aa standard natural multi-activity (Antibacterial, Anticancer, Hemolysis, and other sources) peptide sequence curated from AntiBP3, Peptipedia, HemoPI, and other sources, with an available 3D structural model.

Sequence

KWKKLLKKLLKLLKKLLK

FASTA

>TPDB08725|Antibacterial;Anticancer;Hemolysis;Toxicity|length=18
KWKKLLKKLLKLLKKLLK

Formula C113H208N28O19

Absent Amino Acids ARNDCEQGHIMFPSTYV

Common Amino Acids K

Mass 2263.07

PI 11.41

Basic Residues 9

Acidic Residues 0

Hydrophobic Residues 9

Net Charge +9

Boman Index 2.58

Hydrophobicity -0.311

Aliphatic Index 173.33

Half Life

Mammalian: 1.3 hour Yeast: 3 min E.coli: 2 min

Extinction Coefficient Cystines 5500

Absorbance 280nm 243.03

Polar Residues 0

number

0

A

0

R

0

N

0

D

0

C

0

E

0

Q

0

G

0

H

0

I

8

L

9

K

0

M

0

F

0

P

0

S

0

T

1

W

0

Y

0

V

Amino Acid Distribution

A: 0 R: 0 N: 0 D: 0 C: 0 E: 0 Q: 0 G: 0 H: 0 I: 0 L: 8 K: 9 M: 0 F: 0 P: 0 S: 0 T: 0 W: 1 Y: 0 V: 0

Length

18

Formula

C113H208N28O19

Mass

2263.07

PI

11.41

Net Charge

+9

Boman Index

2.58

Hydrophobicity

-0.311

Evidence 1 Activity

Activity

Activity Endpoint % Hemolysis

Detail Source Activity Hemolysis

Activity Measurement 55 %

Hemolysis Raw

Human erythrocytes: 55% Hemolysis=100 µM

Target

RBC Target Human erythrocytes

Source & Reference

Database Source Hemolytik PD_Hemolysis

Source DRAMP4

Reference

Biochemistry. 2005 Sep 13 44(36):12094-106.

Other

Source Activity Label	Source Definition
Hemolysis	Hemolytik experimentally validated hemolytic peptide annotation.

Evidence 2 Activity

Activity

Activity Endpoint 55% Hemolysis

Detail Source Activity Toxicity

Activity Measurement 100 µM

Toxicity Type Hemolytic Cytotoxic

Toxicity Raw

55% Hemolysis | 100 | µM | target cell: Human erythrocytes

Target

Target Cell Human erythrocytes

Source & Reference

Database Source NA

Source Database DBAASP

Reference

Effects of L- or D-Pro incorporation into hydrophobic or hydrophilic helix face of amphipathic alpha-helical model peptide on structure and cell selectivity. | Biochem Biophys Res Commun | 2004

Evidence 3 Activity

Activity

Activity Endpoint IC50

Detail Source Activity Toxicity

Activity Measurement 1.8 µM

Toxicity Type Cytotoxic

Toxicity Raw

IC50 | 1.8 | µM | target cell: Mouse fibroblasts NIH 3T3

Target

Target Cell Mouse fibroblasts NIH 3T3

Source & Reference

Database Source NA

Source Database DBAASP

Reference

Cell selectivity and mechanism of action of antimicrobial model peptides containing peptoid residues. | Biochemistry | 2005

Evidence 4 Activity

Activity

Activity Endpoint IC50

Detail Source Activity Toxicity

Activity Measurement 1.8 µM

Toxicity Type Hemolytic Cytotoxic

Toxicity Raw

Hemolytic_activity: Human erythrocytes: 55% Hemolysis=100 µM | Cytotoxicity: NIH 3T3: IC50=1.8 µM

Target

Target Cell erythrocytes

Source & Reference

Database Source NA

Source Database DRAMP

Reference

Biochemistry. 2005 Sep 13 44(36):12094-106.

Evidence 5 Hemolysis

Activity

Activity Measurement 55 %

Activity Endpoint % Hemolysis

Hemolysis Raw

Human erythrocytes: 55% Hemolysis=100 µM

Detail Source Activity Hemolysis

Target

RBC Target Human erythrocytes

Source & Reference

source HemoPI PD_Hemolysis

Reference

Biochemistry. 2005 Sep 13 44(36):12094-106.

Source DRAMP4

Database Source PD_Hemolysis