Peptide record

TPDB08319

Ant-anti-gyrA PNA/scPNA Antennapedia(penetratin) Antp D-penetratin L-PEN L-Penetratin L-Penetration nonaarginine (r9) P16 (penetratin) PBA-Penetratin PEN Pen-GFP Penetratin Penetratin (Antennapedia) Penetratin (AP) Penetratin (Pen) PNT PTD1 (Antp) RK16 Antibacterial Anticancer Antifungal Antiviral Blood-Brain Barrier Cell-penetrating Peptides Toxicity standard

16 amino acids

3D PDB MODEL

Drag to rotate. Click a residue or atom to inspect it; the selected residue is highlighted in amber.

TPDB ID TPDB08319

Peptide_Name

Activity

Antibacterial Anticancer Antifungal Antiviral Blood-Brain Barrier Cell-penetrating Peptides Toxicity

Activity_category_statistics

Anti-infective peptides Cancer-related peptides Toxicity and safety peptides Delivery and barrier-penetrating peptides

Database_Source

AntiBP3 dbAMP DRAMP Peptipedia ACP740 _ACP240 AntiCP 2.0 iACP-DRLF pep-lab Antifungipept CAFPdb AI4AVP B3Pred BBPpredict CellPPD CellPPD-MOD CPPsite3.0 PerseuCPP

Sequence_Type

standard

Has_Modification No

Description A 16-aa standard natural multi-activity (Antibacterial, Anticancer, Antifungal, and other sources) peptide sequence curated from AntiBP3, dbAMP, DRAMP, and other sources, with an available 3D structural model.

Sequence

RQIKIWFQNRRMKWKK

FASTA

>TPDB08319|Antibacterial;Anticancer;Antifungal;Antiviral;Blood-Brain Barrier;Cell-penetrating Peptides;Toxicity|length=16
RQIKIWFQNRRMKWKK

Formula C104H168N34O20S1

Absent Amino Acids ADCEGHLPSTYV

Common Amino Acids K

Mass 2246.75

PI 12.72

Basic Residues 7

Acidic Residues 0

Hydrophobic Residues 6

Net Charge +7

Boman Index 2.29

Hydrophobicity -1.731

Aliphatic Index 48.75

Half Life

Mammalian: 1 hour Yeast: 2 min E.coli: 2 min

Extinction Coefficient Cystines 11000

Absorbance 280nm 489.60

Polar Residues 3

number

Amino Acid Distribution

A: 0 R: 3 N: 1 D: 0 C: 0 E: 0 Q: 2 G: 0 H: 0 I: 2 L: 0 K: 4 M: 1 F: 1 P: 0 S: 0 T: 0 W: 2 Y: 0 V: 0

Length

Formula

C104H168N34O20S1

Mass

2246.75

12.72

Net Charge

Boman Index

2.29

Hydrophobicity

-1.731

C_terminal_Modification

Free

Chirality

L D

Conformation

Linear

Ionic_State

Amphipathic Cationic and amphipathic Cationic

Linearity

Linear

N_terminal_Modification

Free

Peptide_Length

Similarity

SMILES

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N[C@@H](CS)C(=O)NCC(=O)N[C@@H](C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](Cc1ccccc1)C(=O)N[C@@H](CC(C)C)C(=O)NCC(=O)N[C@@H](CC1=C(C(=NC1)C)CC)C(=O)N[C@@H](CC(C)C)C(=O)NCC(=O)N[C@@H](C)C(=O)N[C@@H](C)C(=O)NCC(=O)N[C@@H](CO)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CCSC)C(=O)NCC(=O)N[C@@H](C)C(=O)O NCC(=O)N[C@@H](C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](Cc1ccccc1)C(=O)N[C@@H](CC(C)C)C(=O)NCC(=O)N[C@@H](Cc1ccccc1)C(=O)N[C@@H](CC(C)C)C(=O)NCC(=O)N[C@@H](C)C(=O)N[C@@H](C)C(=O)NCC(=O)N[C@@H](CO)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CCSC)C(=O)NCC(=O)N[C@@H](C)C(=O)N[C@@H](CC1=CN=C(C)/C/1=C\C)C(=O)N[C@@H](CO)C(=O)N[C@@H](CCC(=O)N)C(=O)N1CCC[C@H]1C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](C(C)C)C(=O)O N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CC(=O)N)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CC(=O)N)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](CCCN=C)C(=O)O NCC(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC1=CN=C(C)/C/1=C\C)C(=O)N[C@@H](CC1=CN=C(C)/C/1=C\C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC1=CN=C(C)/C/1=C\C)C(=O)N[C@@H](CC1=C(C(=NC1)C)CC)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CO)C(=O)N[C@@H](CC1=CN=C(C)/C/1=C\C)C(=O)N[C@@H](Cc1ccccc1)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC1=C(C(=NC1)C)CC)C(=O)N[C@@H](Cc1ccccc1)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](C)C(=O)O N[C@@H](CC(C)C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC(C)C)C(=O)NCC(=O)NCC(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](C)C(=O)NCC(=O)NCC(=O)N[C@@H](Cc1ccccc1)C(=O)N[C@@H](CC(C)C)C(=O)NCC(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC(C)C)C(=O)NCC(=O)NCC(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](C)C(=O)NCC(=O)O N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCN=C)C(=O)O N[C@@H](CC(C)C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC1=C(C(=NC1)C)CC)C(=O)N[C@@H](CC1=CN=C(C)/C/1=C\C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CC1=CN=C(C)/C/1=C\C)C(=O)N[C@@H](CC1=C(C(=NC1)C)CC)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC(C)C)C(=O)O N[C@@H](CS)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC1=CN=C(C)/C/1=C\C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)O N[C@@H](CCCCN)C(=O)N[C@@H](CCC(=O)N)C(=O)N1CCC[C@H]1C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(=O)O NCC(=O)N[C@@H](C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](Cc1ccccc1)C(=O)N[C@@H](CC(C)C)C(=O)NCC(=O)N[C@@H](Cc1ccccc1)C(=O)N[C@@H](CC(C)C)C(=O)NCC(=O)N[C@@H](C)C(=O)N[C@@H](C)C(=O)NCC(=O)N[C@@H](CO)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CCSC)C(=O)NCC(=O)N[C@@H](C)C(=O)N[C@@H](CC1=CN=C(C)/C/1=C\C)C(=O)N[C@@H](CO)C(=O)N[C@@H](CCC(=O)N)C(=O)N1CCC[C@H]1C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CO)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](C(C)C)C(=O)O N[C@@H](CC(C)C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC(C)C)C(=O)NCC(=O)NCC(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](C)C(=O)NCC(=O)NCC(=O)N[C@@H](CO)C(=O)N[C@@H](CCC(=O)O)C(=O)N[C@@H](Cc1ccccc1)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC(C)C)C(=O)NCC(=O)NCC(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](C)C(=O)NCC(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CO)C(=O)N[C@@H](CO)C(=O)N[C@@H](CC(=O)O)C(=O)N1CCC[C@H]1C(=O)N[C@@H](CC(=O)N)C(=O)N[C@@H](CO)C(=O)N[C@@H](CO)C(=O)N[C@@H](CO)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](C)C(=O)NCC(=O)O

Evidence 1 Activity

Activity

Detail Source Activity Blood-Brain Barrier

Activity Result The intranasal coadministration with L-penetratin has a potential to deliver leptin into the therape

Assay & Model

Animal Model Origin/Source

In Vivo Concentration 2.0 mM

In Vivo Method ELISA

In Vivo Result L-penetratin increased the systemic absorption of leptin after intranasal administration.

Physical Condition Obesity

Source & Reference

Database Source NA

Source B3Pdb

Other

Source Origin Chemically synthesized

In Vivo Mode of Delivery Intranasal

BBB Action The intranasal coadministration with L-penetratin could deliver leptin to the brain and in particula

Transport Type Permeability

Subcellular Localisation Hypothalamus

Combination Combined with leptin

Evidence 2 Activity

Activity

Detail Source Activity Blood-Brain Barrier

Target

Cell Line In situ brain perfusion technique

Assay & Model

In Vitro Method Capillary depletion method

In Vitro Result Vectorization of dox led to a 20-fold increase in the amount of dox transportation.

Animal Model Origin/Source

In Vivo Concentration 2.5 mg/kg

In Vivo Method Capillary depletion method

In Vivo Result It led to a significant increase in brain dox concentrations during the first 30 min of postadminist

Source & Reference

Database Source NA

Source B3Pdb

Other

Source Origin Chemically synthesized

In Vivo Mode of Delivery Intravenous

BBB Action The use of these peptide vectors for brain delivery of dox.

Transport Type Transcytosis

Subcellular Localisation Brain parenchyma

Combination Combined with Doxorubicin

Evidence 3 Activity

Activity

Detail Source Activity Cell-penetrating Peptides

Target

Cell Line PC-3 cells

Source & Reference

Database Source CPPsite3.0

Source CPPsite3

Other

Source Activity Label	Source Definition
Cell-penetrating Peptides	CPPsite3.0 experimentally validated cell-penetrating peptide annotation.

CPP Category Natural residues Linear L Amphipathic

Source of Peptide Protein derived

Cargo Type Protein (eGFP)

Uptake Efficiency Able to penetrate PC-3 cells at 5 mM concentration

Uptake Mechanism Endocytic pathway

In Vitro or In Vivo In vitro

DSSP Secondary Structure CCCSSSTTCCCTTTTC

Evidence 4 Activity

Activity

Detail Source Activity Cell-penetrating Peptides

Assay & Model

In Vitro Model Male Sprague Dawley rats

In Vivo Model Male Sprague Dawley rats

Source & Reference

Database Source CPPsite3.0

Source CPPsite3

Other

CPP Category Natural residues Linear L Amphipathic

Source of Peptide Protein derived

Cargo Type Protein (Insulin)

Uptake Efficiency Less permeability than PenetraMax

In Vitro or In Vivo In vivo

DSSP Secondary Structure CCCSSSTTCCCTTTTC

Evidence 5 Activity

Activity

Detail Source Activity Cell-penetrating Peptides

Assay & Model

In Vitro Model Seven weeks old male ddY mice, male Sprague Dawley rats

In Vivo Model Seven weeks old male ddY mice, male Sprague Dawley rats

Source & Reference

Database Source CPPsite3.0

Source CPPsite3

Other

CPP Category Natural residues Linear L Cationic and amphipathic

Source of Peptide Protein derived

Cargo Type Protein (Insulin)

Uptake Efficiency Higher than control

In Vitro or In Vivo In vivo

DSSP Secondary Structure CCCSSSTTCCCTTTTC

Evidence 6 Activity

Activity

Detail Source Activity Cell-penetrating Peptides

Target

Cell Line CT26 mouse colon cancer cells, HeLa and Hep3B cells

Assay & Model

In Vitro Model Male Balb/c mice

In Vivo Model Male Balb/c mice

Source & Reference

Database Source CPPsite3.0

Source CPPsite3

Other

CPP Category Natural residues Linear L Cationic and amphipathic

Source of Peptide Protein derived

Cargo Type Peptide (Mitochondrial Target Domain of NOXA)

Uptake Efficiency Lower uptake efficiency

In Vitro or In Vivo In vitro and in vivo

DSSP Secondary Structure CCCSSSTTCCCTTTTC

Evidence 7 Activity

Activity

Detail Source Activity Cell-penetrating Peptides

Target

Cell Line HeLa, COS-7, NIH-3T3, Jurkat, NB-4, Kasumi-1, Leishmania tarentolae cell line

Source & Reference

Database Source CPPsite3.0

Source CPPsite3

Other

CPP Category Natural residues Linear L Cationic

Source of Peptide Protein derived

Cargo Type Protein (ATTO488-BSA, ?-galactosidase)

Subcellular Localization Nucleus

In Vitro or In Vivo In vitro

DSSP Secondary Structure CCCSSSTTCCCTTTTC

Evidence 8 Activity

Activity

Detail Source Activity Cell-penetrating Peptides

Assay & Model

In Vitro Model Male ddY mice

In Vivo Model Male ddY mice

Source & Reference

Database Source CPPsite3.0

Source CPPsite3

Other

CPP Category Natural residues Linear L Cationic and amphipathic

Source of Peptide Protein derived

Cargo Type Protein (Insulin)

Uptake Efficiency Higher degradation rate

Uptake Mechanism Energy-independent pathway

In Vitro or In Vivo In vitro

DSSP Secondary Structure CCCSSSTTCCCTTTTC

Evidence 9 Activity

Activity

Detail Source Activity Cell-penetrating Peptides

Target

Cell Line HEK293T cells

Source & Reference

Database Source CPPsite3.0

Source CPPsite3

Other

CPP Category Natural residues Linear L Cationic and amphipathic

Source of Peptide Protein derived

Cargo Type Nucleic acid (Plasmid DNA)

Subcellular Localization Cytoplasm

Uptake Mechanism Endocytosis

In Vitro or In Vivo In vitro

DSSP Secondary Structure CCCSSSTTCCCTTTTC

Evidence 10 Activity

Activity

Detail Source Activity Cell-penetrating Peptides

Assay & Model

In Vitro Model C57BL/6 male mice

In Vivo Model C57BL/6 male mice

Source & Reference

Database Source CPPsite3.0

Source CPPsite3

Other

CPP Category Natural residues Linear L Cationic and amphipathic

Source of Peptide Protein derived

Cargo Type Protein (Single-Chain Antibody)

In Vitro or In Vivo In vivo

DSSP Secondary Structure CCCSSSTTCCCTTTTC

Evidence 11 Activity

Activity

Detail Source Activity Cell-penetrating Peptides

Assay & Model

In Vitro Model Male ddY mice

In Vivo Model Male ddY mice

Source & Reference

Database Source CPPsite3.0

Source CPPsite3

Other

CPP Category Natural residues Linear L Cationic and amphipathic

Source of Peptide Protein derived

Cargo Type Protein (Insulin)

In Vitro or In Vivo In vivo

DSSP Secondary Structure CCCSSSTTCCCTTTTC

Evidence 12 Activity

Activity

Detail Source Activity Cell-penetrating Peptides

Target

Cell Line Jurkat T Clone E6-1, H9 T Cells, PBMCs And MAGI-CCR5

Source & Reference

Database Source CPPsite3.0

Source CPPsite3

Other

CPP Category Natural residues Linear L

Cargo Type anti-p24 mAb

In Vitro or In Vivo In vitro

DSSP Secondary Structure CCCSSSTTCCCTTTTC

Evidence 13 Activity

Activity

Detail Source Activity Cell-penetrating Peptides

Assay & Model

In Vitro Model Male ddY mice, SAMP8 (dementia model mice), and SAMR1 (normal control mice for SAMP8)

In Vivo Model Male ddY mice, SAMP8 (dementia model mice), and SAMR1 (normal control mice for SAMP8)

Source & Reference

Database Source CPPsite3.0

Source CPPsite3

Other

CPP Category Natural residues Linear L Amphipathic

Cargo Type GLP-1 and its analog (exendin-4)

Uptake Efficiency L-penetratin (2mM) enhanced the systemic absorption of both GLP-1 and exendin-4.

In Vitro or In Vivo In vivo

DSSP Secondary Structure CCCSSSTTCCCTTTTC

Evidence 14 Activity

Activity

Detail Source Activity Cell-penetrating Peptides

Target

Cell Line Caco-2, HeLa, HEPG2 And IEC-6 Cells

Source & Reference

Database Source CPPsite3.0

Source CPPsite3

Other

CPP Category Natural residues Linear L Cationic

Source of Peptide Protein derived

Subcellular Localization Cytoplasm

In Vitro or In Vivo In vitro

DSSP Secondary Structure CCCSSSTTCCCTTTTC

Evidence 15 Activity

Activity

Detail Source Activity Cell-penetrating Peptides

Target

Cell Line Caco-2, HeLa, HEPG2 And IEC-6 Cells

Source & Reference

Database Source CPPsite3.0

Source CPPsite3

Other

CPP Category Natural residues Linear L Cationic

Source of Peptide Protein derived

In Vitro or In Vivo In vitro

DSSP Secondary Structure CCCSSSTTCCCTTTTC

Evidence 16 Activity

Activity

Detail Source Activity Cell-penetrating Peptides

Assay & Model

In Vitro Model Male SAMP8 (dementia model mice) and SAMR1 (normal control mice)

In Vivo Model Male SAMP8 (dementia model mice) and SAMR1 (normal control mice)

Source & Reference

Database Source CPPsite3.0

Source CPPsite3

Other

CPP Category Natural residues Linear L

Source of Peptide Protein derived

Cargo Type Insulin

Uptake Efficiency L-penetratin increased the plasma insulin concentration more than d-penetratin

In Vitro or In Vivo In vivo

DSSP Secondary Structure CCCSSSTTCCCTTTTC

Evidence 17 Activity

Activity

Detail Source Activity Cell-penetrating Peptides

Target

Cell Line Caco-2 Cell

Assay & Model

In Vitro Model Male Sprague Dawley rats weighing 180–220 g and male ddY mice weighing 30–40 g

In Vivo Model Male Sprague Dawley rats weighing 180–220 g and male ddY mice weighing 30–40 g

Source & Reference

Database Source CPPsite3.0

Source CPPsite3

Other

CPP Category Natural residues Linear L Amphipathic

Source of Peptide Protein derived

Cargo Type Insulin

In Vitro or In Vivo In vitro and in vivo

DSSP Secondary Structure CCCSSSTTCCCTTTTC

Evidence 18 Activity

Activity

Detail Source Activity Cell-penetrating Peptides

Target

Cell Line Intestinal Loops Of Sprague Dawley Rats

Source & Reference

Database Source CPPsite3.0

Source CPPsite3

Other

CPP Category Natural residues Linear L

Source of Peptide Synthetic

Cargo Type Insulin

Uptake Efficiency L- and D-PenetraMax show greatest increase in ileum insulin absorption than other CPP

In Vitro or In Vivo In Situ

DSSP Secondary Structure CCCSSSTTCCCTTTTC

Evidence 19 Activity

Activity

Detail Source Activity Cell-penetrating Peptides

Target

Cell Line Vero Cells And Hk-2 Cells

Source & Reference

Database Source CPPsite3.0

Source CPPsite3

Other

CPP Category Natural residues Linear L Amphipathic

Source of Peptide Protein derived

Cargo Type Copper/zinc superoxide dismutase (SOD1)

Uptake Efficiency PEN-SOD1 internalization was comparable to TAT-SOD1 and R10- SOD1

In Vitro or In Vivo In vitro

DSSP Secondary Structure CCCSSSTTCCCTTTTC

Evidence 20 Activity

Activity

Detail Source Activity Cell-penetrating Peptides

Target

Cell Line HeLa Cells

Source & Reference

Database Source CPPsite3.0

Source CPPsite3

Other

CPP Category Natural residues Linear L Amphipathic

Source of Peptide Protein derived

Cargo Type FITC-labeled

Subcellular Localization Cytosol, Nucleus and Endosomes

Uptake Efficiency Show considerably lower uptake

In Vitro or In Vivo In vitro

DSSP Secondary Structure CCSSCCCCCCCCCCCSSCCCC

Evidence 21 Activity

Activity

Detail Source Activity Cell-penetrating Peptides

Assay & Model

In Vitro Model Male Sprague-Dawley rats weighing 200–220 g

In Vivo Model Male Sprague-Dawley rats weighing 200–220 g

Source & Reference

Database Source CPPsite3.0

Source CPPsite3

Other

CPP Category Natural residues Linear L Amphipathic

Source of Peptide Protein derived

Cargo Type 64Cu-NODAGA–insulin

Uptake Efficiency L-penetration caused the greatest increase in the radioactive signal in the brain.

In Vitro or In Vivo In vivo

DSSP Secondary Structure CCCCTHHHHHHHTTSSCC

Evidence 22 Activity

Activity

Detail Source Activity Cell-penetrating Peptides

Target

Cell Line Caco-2 Cells

Source & Reference

Database Source CPPsite3.0

Source CPPsite3

Other

CPP Category Natural residues Linear L Amphipathic

Source of Peptide Protein derived

Cargo Type Insulin

Uptake Efficiency Enhanced permeability of insulin with L-penetratin than D-penetratin

In Vitro or In Vivo In vitro

DSSP Secondary Structure CCCSSSTTCCCTTTTC

Evidence 23 Activity

Activity

Detail Source Activity Cell-penetrating Peptides

Target

Cell Line S. Aureus Infected Mac-T Or Hbmec Cell Lines , Ex Vivo Calvaria (Skull Cap)

Assay & Model

In Vitro Model Femur injury model (Wistar rats (5 to 6 wks of age))

In Vivo Model Femur injury model (Wistar rats (5 to 6 wks of age))

Source & Reference

Database Source CPPsite3.0

Source CPPsite3

Other

CPP Category Natural residues Linear L

Source of Peptide Protein derived

Cargo Type Lysostaphin and LysK

In Vitro or In Vivo In vitro and in vivo

DSSP Secondary Structure CCCCSSCCSCC

Evidence 24 Activity

Activity

Detail Source Activity Cell-penetrating Peptides

Target

Cell Line Hek293T And Ct26.Cl25 Cell Lines

Source & Reference

Database Source CPPsite3.0

Source CPPsite3

Other

CPP Category Natural residues Linear L Amphipathic

Source of Peptide Protein derived

Cargo Type 5-methoxyuridine-modified mCherry mRNA, Enhanced green fluorescence protein (EGFP) mRNA and Ovalbumin (OVA) mRNA

Subcellular Localization Cytoplasm

Uptake Efficiency Penetratin, showed a low cellular uptake of 28.7%

In Vitro or In Vivo In vitro

DSSP Secondary Structure CCSSCCCCCCCCCCCSSCCCC

Evidence 25 Activity

Activity

Detail Source Activity Cell-penetrating Peptides

Target

Cell Line S. Pyogenes Strains

Assay & Model

In Vitro Model G. mellonella Larvae

In Vivo Model G. mellonella Larvae

Source & Reference

Database Source CPPsite3.0

Source CPPsite3

Other

CPP Category Non-natural residues Linear L

Source of Peptide Protein derived

Cargo Type anti-gyrA antisense PNAs and scrambled PNAs [scPNAs]

Uptake Efficiency

Penetratin (Ant) did not support antisense PNA uptake into S. pyogenes. Also did not affect the survival of infected larvae.

In Vitro or In Vivo In vitro and in vivo

Evidence 26 Activity

Activity

Detail Source Activity Cell-penetrating Peptides

Target

Cell Line HeLa Cells

Source & Reference

Database Source CPPsite3.0

Source CPPsite3

Other

CPP Category Natural residues Linear L

Source of Peptide Protein derived

Cargo Type SIP-U1

In Vitro or In Vivo In vitro

DSSP Secondary Structure CCTTSSCCCCC

Evidence 27 Activity

Activity

Detail Source Activity Cell-penetrating Peptides

Target

Cell Line P. Berghei (Pb), P. Yoelii (Py), And P. Falciparum (Pf) Sporozoites

Source & Reference

Database Source CPPsite3.0

Source CPPsite3

Other

CPP Category Natural residues Linear L

Source of Peptide Protein derived

In Vitro or In Vivo In vitro

DSSP Secondary Structure CCCC

Evidence 28 Activity

Activity

Detail Source Activity Cell-penetrating Peptides

Target

Cell Line Ai9 Tdtomato Npcs

Assay & Model

In Vitro Model Ai9 or wild-type mice

In Vivo Model Ai9 or wild-type mice

Source & Reference

Database Source CPPsite3.0

Source CPPsite3

Other

CPP Category Natural residues Linear L

Cargo Type SpyCas9 RNPs

In Vitro or In Vivo In vitro and in vivo

DSSP Secondary Structure CCSSSCCSSCSSSSSCTTTIIIIIHHHGGGTSCCC

Evidence 29 Activity

Activity

Detail Source Activity Cell-penetrating Peptides

Target

Cell Line E. Coli , C. Crescentus Cells

Source & Reference

Database Source CPPsite3.0

Source CPPsite3

Other

CPP Category Natural residues Linear L Amphipathic

Source of Peptide Protein derived

Cargo Type SpyCatcher003 at C-terminus of CPP via GGGS spacer

Subcellular Localization Cytoplasm

Uptake Efficiency Mean AF647 fluorescence per cell increased with the Penetratin-SpyCatcher fusion protein versus the SpyCatcher alone.

In Vitro or In Vivo In vitro

DSSP Secondary Structure CCCCCCCCCCSSSSCC

Evidence 30 Activity

Activity

Detail Source Activity Cell-penetrating Peptides

Target

Cell Line Be(2)-C , Sk-N-Dz And SH-SY5Y

Assay & Model

In Vitro Model 6–8 weeks old female NCr nude mice (Taconic).

In Vivo Model 6–8 weeks old female NCr nude mice (Taconic).

Source & Reference

Database Source CPPsite3.0

Source CPPsite3

Other

CPP Category Non-natural residues Linear L Amphipathic

Source of Peptide Protein derived

Cargo Type dominant-negative ATF5 sequence

In Vitro or In Vivo In vitro and in vivo

Evidence 31 Activity

Activity

Detail Source Activity Cell-penetrating Peptides

Target

Cell Line Hek 293T Cells,Hek-Blue Il-1R Cells, THP-1 Cells, Primary Human Monocytes

Assay & Model

In Vitro Model C57BL/6J mice

In Vivo Model C57BL/6J mice

Source & Reference

Database Source CPPsite3.0

Source CPPsite3

Other

CPP Category Natural residues Linear L

Cargo Type SLAMF1-derived peptide P7

Uptake Efficiency

Both P7-Arg11 and P7-Pen [but not Pen and Arg11 or P7 without CPP], significantly reduced LPS-mediated IFNβ and TNF mRNA expression), with P7-Pen being the most potent inhibitor.

In Vitro or In Vivo In vitro and in vivo

DSSP Secondary Structure CCCGGGGCCCC

Evidence 32 Activity

Activity

Detail Source Activity Cell-penetrating Peptides

Target

Cell Line Primary Astrocytes And Neuronal Cells.

Assay & Model

In Vitro Model C57BL6/J mice

In Vivo Model C57BL6/J mice

Source & Reference

Database Source CPPsite3.0

Source CPPsite3

Other

CPP Category Natural residues Linear L Cationic

Source of Peptide Protein derived

Cargo Type pDNA (pApoE2)

Uptake Efficiency

The OA-g-CS-PEN-MAN/pApoE2 polyplex demonstrated significantly (p < 0.05) 10.9 times higher transfection of pApoE2 in the primary astrocytes over naked pApoE2. And in mice was ≈1.63 times higher.

In Vitro or In Vivo In vitro and in vivo

DSSP Secondary Structure CCCCCCCCC

Evidence 33 Activity

Activity

Detail Source Activity Cell-penetrating Peptides

Assay & Model

In Vitro Model Acyrthosiphon pisum (pea aphid)

In Vivo Model Acyrthosiphon pisum (pea aphid)

Source & Reference

Database Source CPPsite3.0

Source CPPsite3

Other

CPP Category Natural residues Linear L Amphipathic

Source of Peptide Protein derived

Cargo Type mVenus

Subcellular Localization Aphid Hemolymph

Uptake Efficiency

mVenus-PEN fluorescence was observed 24 h after injection, but no signals were observed in any tissue using mVenus without PEN.

In Vitro or In Vivo In vivo

DSSP Secondary Structure CCSTTTCSCTTTC

Evidence 34 Activity

Activity

Detail Source Activity Cell-penetrating Peptides

Target

Cell Line Skov-3 And HeLa Cells

Assay & Model

In Vitro Model 6 week-old female BALB/c mice

In Vivo Model 6 week-old female BALB/c mice

Source & Reference

Database Source CPPsite3.0

Source CPPsite3

Other

CPP Category Natural residues Linear L

Source of Peptide Protein derived

Cargo Type FITC-labeled Mn:ZnS Nanoparticels loaded with Paclitaxel (PTX)

Uptake Efficiency

Intracellular fluorescence was improved with R9-modified NPs compared to the PEN and pVEC which was confirmed by flow cytometry

In Vitro or In Vivo In vitro and in vivo

DSSP Secondary Structure CCSSCSSCC

Evidence 35 Activity

Activity

Detail Source Activity Cell-penetrating Peptides

Target

Cell Line Caco-2 And Ht29 Cells

Assay & Model

In Vitro Model Male/Female SD rats

In Vivo Model Male/Female SD rats

Source & Reference

Database Source CPPsite3.0

Source CPPsite3

Other

CPP Category Non-natural residues Linear L

Cargo Type FITC-labeled large-porous Mesostructured silica nanoparticle (NP)

Subcellular Localization Lysosome and Endoplasmic Reticulum

Uptake Efficiency

At 40% NPs ratio of CPP@LMSN , the intracellular fluorescence intensity reached a maximum, which was 3.83-fold more than that of blank LMSN without CPP@NP involvement in caco-2 cells

Uptake Mechanism Clathrin-mediated endocytosis and other active uptake mechanisms

In Vitro or In Vivo In vitro and in vivo

Evidence 36 Activity

Activity

Detail Source Activity Cell-penetrating Peptides

Assay & Model

In Vitro Model Male ddY mice and male Sprague-Dawley rats

In Vivo Model Male ddY mice and male Sprague-Dawley rats

Source & Reference

Database Source CPPsite3.0

Source CPPsite3

Other

CPP Category Natural residues Linear L Amphipathic

Cargo Type Exendin-4

Uptake Efficiency

High Exendin-4 levels was detected in brain regions after intranasal administration with L-penetratin and low levels in the olfactory bulb even without L-penetratin

In Vitro or In Vivo In vivo and ex vivo

DSSP Secondary Structure CTTTTHHHHHHHHTTTCC

Evidence 37 Activity

Activity

Detail Source Activity Cell-penetrating Peptides

Target

Cell Line Bbecs Or Rat Astrocytes And Intestine (Caco-2 Assay) And A Bbb Model

Source & Reference

Database Source CPPsite3.0

Source CPPsite3

Other

CPP Category Natural residues Linear L

Source of Peptide Protein derived

Cargo Type carboxyfuorescein (Cf) at N -terminal

Uptake Efficiency

Three CPPs (TAT, penetratin and R8) showed the highest internalization profiles in both cell types.Penetratin crossed the intestinal barrier.

In Vitro or In Vivo In vitro

DSSP Secondary Structure CCCCCCTTCC

Evidence 38 Activity

Activity

Detail Source Activity Cell-penetrating Peptides

Target

Cell Line Caco-2 Cells

Assay & Model

In Vitro Model Male Sprague-Dawley rats (252±7 g)

In Vivo Model Male Sprague-Dawley rats (252±7 g)

Source & Reference

Database Source CPPsite3.0

Source CPPsite3

Other

CPP Category Natural residues Linear L Cationic

Source of Peptide Protein derived

Cargo Type Insulin

In Vitro or In Vivo In vitro and in vivo

DSSP Secondary Structure CCCCSSCSCCGGGCC

Evidence 39 Activity

Activity

Detail Source Activity Cell-penetrating Peptides

Assay & Model

In Vitro Model Seven weeks old male ddY mice (30–36 g)

In Vivo Model Seven weeks old male ddY mice (30–36 g)

Source & Reference

Database Source CPPsite3.0

Source CPPsite3

Other

CPP Category Natural residues Linear L Cationic

Source of Peptide Protein derived

In Vitro or In Vivo In vivo

DSSP Secondary Structure CCCGGGGSCCCCCCTTTSCSSSSSSSSCCCCSCC

Evidence 40 Activity

Activity

Detail Source Activity Cell-penetrating Peptides

Target

Cell Line Wheat Microspores

Source & Reference

Database Source CPPsite3.0

Source CPPsite3

Other

CPP Category Natural residues Linear L Cationic

Source of Peptide Protein derived

Cargo Type Cys-mCherry protein

Uptake Efficiency Cationic CPPs demonstrate the highest binding as compared to amphipathic ones

In Vitro or In Vivo In vitro

DSSP Secondary Structure CCCCCCCCCCCCC

Evidence 41 Activity

Activity

Detail Source Activity Cell-penetrating Peptides

Assay & Model

In Vitro Model P. falciparum trophozoite stage cells

In Vivo Model P. falciparum trophozoite stage cells

Source & Reference

Database Source CPPsite3.0

Source CPPsite3

Other

CPP Category Natural residues Linear L Cationic

Source of Peptide Protein derived

Cargo Type SYBR green-I, doxorubicin and actinomycin D

In Vitro or In Vivo In vivo

DSSP Secondary Structure CCCCCCCSCC

Evidence 42 Activity

Activity

Detail Source Activity Cell-penetrating Peptides

Target

Cell Line Caco-2

Source & Reference

Database Source CPPsite3.0

Source CPPsite3

Other

CPP Category Natural residues Linear L Cationic

Source of Peptide Protein derived

Cargo Type Nanoparticles and FITC labelled dextrans

Uptake Efficiency

R8 and Tat increased the uptake of 200-nm nanoparticles by Caco-2 cells to 2.1-3.0 times that was similar or superior than penetratin and PenetraMax

In Vitro or In Vivo In vitro

DSSP Secondary Structure CCSCTTSCCCCC

Evidence 43 Activity

Activity

Detail Source Activity Cell-penetrating Peptides

Target

Cell Line Ccl-20.2 Freshly Enucleated Pig Eyes

Source & Reference

Database Source CPPsite3.0

Source CPPsite3

Other

CPP Category Non-natural residues Linear L Cationic

Source of Peptide Protein derived

Cargo Type 5-carboxyfluorescein (5-FAM)

Subcellular Localization Cytoplasm and nucleus

Uptake Efficiency Permeability coefficient of 20-fold higher than 5-FAM

In Vitro or In Vivo In vitro and ex vivo

Evidence 44 Activity

Activity

Detail Source Activity Cell-penetrating Peptides

Target

Cell Line Ma-104 And Caco-2

Source & Reference

Database Source CPPsite3.0

Source CPPsite3

Other

CPP Category Natural residues Linear L Cationic

Source of Peptide Protein derived

Cargo Type Fluorescein and rhodamine B dye

Subcellular Localization Cytoplasm

Uptake Mechanism Endocytosis

In Vitro or In Vivo In vitro

DSSP Secondary Structure CCBTTBSSCC

Evidence 45 Activity

Activity

Detail Source Activity Cell-penetrating Peptides

Target

Cell Line Nih/3T3, CHO-K1, Or HeLa Cells

Source & Reference

Database Source CPPsite3.0

Source CPPsite3

Other

CPP Category Natural residues Linear L Cationic

Source of Peptide Protein derived

Cargo Type TfAF488 or 0.2-μm microspheres , lentivirus particles carrying the pac gene

Uptake Efficiency PepB2 show greater efficacy than other

In Vitro or In Vivo In vitro

DSSP Secondary Structure CCCCSCCCCSSCSSSSCC

Evidence 46 Activity

Activity

Detail Source Activity Cell-penetrating Peptides

Target

Cell Line Monomac6 Human Monocytes

Source & Reference

Database Source CPPsite3.0

Source CPPsite3

Other

CPP Category Natural residues Linear L Cationic

Source of Peptide Protein derived

Uptake Efficiency Cellular uptake slightly affected by CF-labelling

In Vitro or In Vivo In vitro

DSSP Secondary Structure CCSCCCSSSSCCCCCCCCCC

Evidence 47 Activity

Activity

Detail Source Activity Cell-penetrating Peptides

Target

Cell Line Caco-2, Hepg2, Iec-6

Source & Reference

Database Source CPPsite3.0

Source CPPsite3

Other

CPP Category Natural residues Linear L Amphipathic

Source of Peptide Protein derived

In Vitro or In Vivo In vitro

DSSP Secondary Structure CCCCCCGGGTTSSSCSCC

Evidence 48 Activity

Activity

Detail Source Activity Cell-penetrating Peptides

Assay & Model

In Vitro Model Seven-week-old male ddY mice weighing 35–40 g

In Vivo Model Seven-week-old male ddY mice weighing 35–40 g

Source & Reference

Database Source CPPsite3.0

Source CPPsite3

Other

CPP Category Natural residues Linear L Amphipathic

Source of Peptide Protein derived

Cargo Type Insulin

Uptake Efficiency Less efficient than D form

In Vitro or In Vivo In vivo

DSSP Secondary Structure CCCBTTBCCCCSSCCCCCCCSSCCCCC

Evidence 49 Activity

Activity

Detail Source Activity Cell-penetrating Peptides

Target

Cell Line HeLa Pluc705 Cells , RAW264.7, Hepg2, And MCF-7 Cells

Source & Reference

Database Source CPPsite3.0

Source CPPsite3

Other

CPP Category Natural residues Linear L Amphipathic

Source of Peptide Protein derived

Cargo Type PNA705, tetramethylrhodamine (TAMRA) and GFP11 peptide

Subcellular Localization Cytoplasm

Uptake Efficiency

15-fold increase over background of untreated cells and TAMRA delivery follows P17 >>P11 = P14 > P40 = Arg9 > tat = penetratin

Uptake Mechanism Direct translocation and endocytosis

In Vitro or In Vivo In vitro

DSSP Secondary Structure CCSSCCCCCCCCCCCSSCCCC

Evidence 50 Activity

Activity

Detail Source Activity Cell-penetrating Peptides

Assay & Model

In Vitro Model Normal BL6 mice, dystrophin-null mdx4cv mice and ∆R4 transgenic mice

In Vivo Model Normal BL6 mice, dystrophin-null mdx4cv mice and ∆R4 transgenic mice

Source & Reference

Database Source CPPsite3.0

Source CPPsite3

Other

CPP Category Non-natural residues Linear L

Source of Peptide Protein derived

Cargo Type Palmitoylated (Pal) R16/17-GFP protein

In Vitro or In Vivo In vivo

Evidence 51 Activity

Activity

Detail Source Activity Cell-penetrating Peptides

Target

Cell Line Hek-293T Cells

Assay & Model

In Vitro Model 7 to 7 week-old female BALB/c mice

In Vivo Model 7 to 7 week-old female BALB/c mice

Source & Reference

Database Source CPPsite3.0

Source CPPsite3

Other

CPP Category Natural residues Linear L Amphipathic

Source of Peptide Protein derived

Cargo Type Protein (HIV-1 Nef or Hsp20-Nef proteins) and FITC-antibody

Uptake Efficiency Effectively delivered protein

In Vitro or In Vivo In vitro and in vivo

DSSP Secondary Structure CCSCCSSCCCSSSCC

Evidence 52 Activity

Activity

Detail Source Activity Cell-penetrating Peptides

Assay & Model

In Vitro Model Male Wistar rats weighing 180–220 g

In Vivo Model Male Wistar rats weighing 180–220 g

Source & Reference

Database Source CPPsite3.0

Source CPPsite3

Other

CPP Category Natural residues Linear L Amphipathic

Source of Peptide Protein derived

Cargo Type Insulin-loaded solid lipid nanoparticles (INS-SLNs)

Uptake Efficiency Most effective SLNs

In Vitro or In Vivo In vivo

DSSP Secondary Structure CCCCCCCTHHHHHHHHHSCC

Evidence 53 Activity

Activity

Detail Source Activity Cell-penetrating Peptides

Target

Cell Line Caco-2, Raji Cells

Source & Reference

Database Source CPPsite3.0

Source CPPsite3

Other

CPP Category Natural residues Linear L Amphipathic

Source of Peptide Protein derived

Cargo Type Insulin

Uptake Efficiency Effect of L-penetratin on epithelial permeation of insulin was stronger than that of D-R8

In Vitro or In Vivo In vitro

DSSP Secondary Structure CHHHHHHHHHHHHHHHHHHHCCTHHHHCCTTHHHHHTTCCHHHHHTTTTHHHHHHHHHHHHHHGGGCC

Evidence 54 Activity

Activity

Detail Source Activity Cell-penetrating Peptides

Target

Cell Line

Caco-2 Cells Bacteroidetes (Bacteroides Vulgatus And Bacteroides Thetaiotaomicron), Firmicutes (Lactobacillus Gasseri, Latilactobacillus Sakei, And Clostridium Bolteae), Actinomicetya (Bifidobacterium Longum, And Bifidobacterium Adolescentis) , Proteobacteria (Escherichia Coli Nissle 1917 And Escherichia Coli K12) And Saccharomyces Boulardii

Source & Reference

Database Source CPPsite3.0

Source CPPsite3

Other

CPP Category Natural residues Linear L

Source of Peptide Protein derived

Cargo Type FITC-dextran

In Vitro or In Vivo In vitro

DSSP Secondary Structure CGGGGTHHHHHHHHHHHC

Evidence 55 Activity

Activity

Detail Source Activity Cell-penetrating Peptides

Target

Cell Line HeLa pLuc705 cells

Source & Reference

Database Source CPPsite3.0

Source CPPsite3

Other

CPP Category Non-natural residues Linear L

Source of Peptide Protein derived

Cargo Type SR-A3s and SR-A5s

Subcellular Localization Cytoplasm

In Vitro or In Vivo In vitro

Evidence 56 Activity

Activity

Detail Source Activity Cell-penetrating Peptides

Target

Cell Line Caco-2 cells

Assay & Model

In Vitro Model Male Sprague–Dawley rats (180–220 g)

In Vivo Model Male Sprague–Dawley rats (180–220 g)

Source & Reference

Database Source CPPsite3.0

Source CPPsite3

Other

CPP Category Natural residues Linear L Amphipathic

Source of Peptide Protein derived

Cargo Type Drugs (metformin, risedronate, zanamivir, methotrexate [MTX], tacrolimus, and vincristine [VCR])

Uptake Efficiency L- and D-penetratin significantly increases the epithelial permeation of MTX and VCR

In Vitro or In Vivo In vitro and in vivo

DSSP Secondary Structure CCHHHHHHHHHHTTTC

Evidence 57 Activity

Activity

Detail Source Activity Cell-penetrating Peptides

Assay & Model

In Vitro Model Female BALB/c mice (6-9 weeks)

In Vivo Model Female BALB/c mice (6-9 weeks)

Source & Reference

Database Source CPPsite3.0

Source CPPsite3

Other

CPP Category Natural residues Linear L Amphipathic

Source of Peptide Protein derived

Cargo Type OVA or Influenza A Virus

Uptake Efficiency Stronger effect of D-penetratin compared to L-penetratin

In Vitro or In Vivo In vivo

DSSP Secondary Structure CHHHHHHHHHHHHHHTCC

Evidence 58 Activity

Activity

Detail Source Activity Cell-penetrating Peptides

Target

Cell Line HeLa cells

Source & Reference

Database Source CPPsite3.0

Source CPPsite3

Other

CPP Category Natural residues Linear L

Source of Peptide Protein derived

Cargo Type PLGA NPs

Uptake Efficiency Low uptake

In Vitro or In Vivo In vitro

DSSP Secondary Structure CCCCCCCCC

Evidence 59 Activity

Activity

Detail Source Activity Cell-penetrating Peptides

Target

Cell Line A431, HEK293 and SKOV3 cells

Assay & Model

In Vitro Model Female BALB/c nude mice (6–8 weeks old and 20 g)

In Vivo Model Female BALB/c nude mice (6–8 weeks old and 20 g)

Source & Reference

Database Source CPPsite3.0

Source CPPsite3

Other

CPP Category Natural residues Linear D Cationic

Source of Peptide Synthetic

Cargo Type 7D12 anti-EGFR nanobody

Uptake Efficiency r9 and pen had outperformed their L-analogues

In Vitro or In Vivo In vitro and in vivo

DSSP Secondary Structure CCCCTTTTTTSSCSSSSSSSSSSSSCC

Evidence 60 Activity

Activity

Detail Source Activity Cell-penetrating Peptides

Target

Cell Line HEI-OC1 cells

Assay & Model

In Vitro Model Guinea pigs (250–300 g)

In Vivo Model Guinea pigs (250–300 g)

Source & Reference

Database Source CPPsite3.0

Source CPPsite3

Other

CPP Category Natural residues Linear L Amphipathic

Source of Peptide Protein derived

Cargo Type coumarin-6-loaded PLGA-NPs and P407-PLGA-NPs

Uptake Efficiency LMWP group had stronger fluorescence, followed by penetratin, TAT and R8 groups

In Vitro or In Vivo In vitro and in vivo

DSSP Secondary Structure CTTGGGTTHHHHHHHHHHHHHHHHHHHTTSSHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHSCCSSSTTSCGGGGGGTHHHHHHTTCC

Evidence 61 Activity

Activity

Activity Endpoint 0% Hemolysis

Detail Source Activity Toxicity

Activity Measurement 50 µM

Toxicity Type Hemolytic Cytotoxic

Toxicity Raw

0% Hemolysis | 50 | µM | target cell: Rat erythrocytes

Target

Target Cell Rat erythrocytes

Source & Reference

Database Source NA

Source Database DBAASP

Reference

Lipid reorganization induced by membrane-active peptides probed using differential scanning calorimetry. | Biochim Biophys Acta | 2009

Evidence 62 Activity

Activity

Activity Endpoint IC50

Detail Source Activity Toxicity

Activity Measurement >256 µM

Toxicity Type Cytotoxic

Toxicity Raw

IC50 | >256 | µM | target cell: Murine macrophage cells J774A.1

Target

Target Cell Murine macrophage cells J774A.1

Source & Reference

Database Source NA

Source Database DBAASP

Reference

Antibacterial Activity of Novel Cationic Peptides against Clinical Isolates of Multi-Drug Resistant Staphylococcus pseudintermedius from Infected Dogs. | PLoS One | 2014

Evidence 63 Activity

Activity

Activity Endpoint IC50

Detail Source Activity Toxicity

Activity Measurement >256 µM

Toxicity Type Cytotoxic

Toxicity Raw

IC50 | >256 | µM | target cell: Human keratinocytes HaCat

Target

Target Cell Human keratinocytes HaCat

Source & Reference

Database Source NA

Source Database DBAASP

Reference

Antibacterial Activity of Novel Cationic Peptides against Clinical Isolates of Multi-Drug Resistant Staphylococcus pseudintermedius from Infected Dogs. | PLoS One | 2014

Evidence 64 Activity

Activity

Activity Endpoint 50% Hemolysis

Detail Source Activity Toxicity

Activity Measurement >128 µM

Toxicity Type Hemolytic Cytotoxic

Toxicity Raw

50% Hemolysis | >128 | µM | target cell: Human erythrocytes

Target

Target Cell Human erythrocytes

Source & Reference

Database Source NA

Source Database DBAASP

Reference

Antimicrobial and cell-penetrating properties of penetratin analogs: effect of sequence and secondary structure. | Biochim Biophys Acta | 2013

Evidence 65 Activity

Activity

Activity Endpoint 5% Hemolysis

Detail Source Activity Toxicity

Activity Measurement >200 µM

Toxicity Type Hemolytic Cytotoxic

Toxicity Raw

5% Hemolysis | >200 | µM | target cell: Human erythrocytes

Target

Target Cell Human erythrocytes

Source & Reference

Database Source NA

Source Database DBAASP

Reference

Antimicrobial and cytolytic activities and plausible mode of bactericidal action of the cell penetrating peptide penetratin and its lys-linked two-stranded peptide. | Chem Biol Drug Des | 2009

Evidence 66 Activity

Activity

Activity Endpoint 50% Cell death

Detail Source Activity Toxicity

Activity Measurement >100 µM

Toxicity Type Cytotoxic

Toxicity Raw

50% Cell death | >100 | µM | target cell: Mouse fibroblasts NIH 3T3

Target

Target Cell Mouse fibroblasts NIH 3T3

Source & Reference

Database Source NA

Source Database DBAASP

Reference

Antimicrobial and cytolytic activities and plausible mode of bactericidal action of the cell penetrating peptide penetratin and its lys-linked two-stranded peptide. | Chem Biol Drug Des | 2009

Evidence 67 Activity

Activity

Activity Endpoint IC50

Detail Source Activity Toxicity

Activity Measurement 136±25.6 µM

Toxicity Type Cytotoxic

Toxicity Raw

IC50 | 136±25.6 | µM | target cell: Human PBMC

Target

Target Cell Human PBMC

Source & Reference

Database Source NA

Source Database DBAASP

Reference

Comparative analysis of internalisation, haemolytic, cytotoxic and antibacterial effect of membrane-active cationic peptides: aspects of experimental setup. | Amino Acids | 2017

Evidence 68 Activity

Activity

Activity Endpoint 50% Hemolysis

Detail Source Activity Toxicity

Activity Measurement >300 µM

Toxicity Type Hemolytic Cytotoxic

Toxicity Raw

50% Hemolysis | >300 | µM | target cell: Human erythrocytes

Target

Target Cell Human erythrocytes

Source & Reference

Database Source NA

Source Database DBAASP

Reference

Comparative analysis of internalisation, haemolytic, cytotoxic and antibacterial effect of membrane-active cationic peptides: aspects of experimental setup. | Amino Acids | 2017

Evidence 69 Activity

Activity

Activity Endpoint 0% Hemolysis

Detail Source Activity Toxicity

Activity Measurement 100 µg/mL

Toxicity Type Hemolytic Cytotoxic

Toxicity Raw

0% Hemolysis | 100 | µg/mL | target cell: Human erythrocytes

Target

Target Cell Human erythrocytes

Source & Reference

Database Source NA

Source Database DBAASP

Reference

A Foundation Model Identifies Broad-Spectrum Antimicrobial Peptides against Drug-Resistant Bacterial Infection | Nat Commun | 2024

Evidence 70 Activity

Activity

Activity Endpoint IC50

Detail Source Activity Toxicity

Activity Measurement >50 µg/mL

Toxicity Type Cytotoxic

Toxicity Raw

IC50 | >50 | µg/mL | target cell: Human dermal fibroblasts

Target

Target Cell Human dermal fibroblasts

Source & Reference

Database Source NA

Source Database DBAASP

Reference

A Foundation Model Identifies Broad-Spectrum Antimicrobial Peptides against Drug-Resistant Bacterial Infection | Nat Commun | 2024

Evidence 71 Activity

Activity

Activity Endpoint 5% Hemolysis

Detail Source Activity Toxicity

Activity Measurement 25 µM

Toxicity Type Hemolytic Cytotoxic

Toxicity Raw

5% Hemolysis | 25 | µM | target cell: Human erythrocytes

Target

Target Cell Human erythrocytes

Source & Reference

Database Source NA

Source Database DBAASP

Reference

Antimicrobial and cytolytic activities and plausible mode of bactericidal action of the cell penetrating peptide penetratin and its lys-linked two-stranded peptide. | Chem Biol Drug Des | 2009

Evidence 72 Activity

Activity

Activity Endpoint 50% Cell death

Detail Source Activity Toxicity

Activity Measurement 32 µM

Toxicity Type Cytotoxic

Toxicity Raw

50% Cell death | 32 | µM | target cell: Mouse fibroblasts NIH 3T3

Target

Target Cell Mouse fibroblasts NIH 3T3

Source & Reference

Database Source NA

Source Database DBAASP

Reference

Antimicrobial and cytolytic activities and plausible mode of bactericidal action of the cell penetrating peptide penetratin and its lys-linked two-stranded peptide. | Chem Biol Drug Des | 2009

DSSP_Secondary_Structure

CCCSSSTTCCCTTTTC CCSSCCCCCCCCCCCSSCCCC CCCCTHHHHHHHTTSSCC CCCCSSCCSCC CCTTSSCCCCC CCCC CCSSSCCSSCSSSSSCTTTIIIIIHHHGGGTSCCC CCCCCCCCCCSSSSCC CCCGGGGCCCC CCCCCCCCC CCSTTTCSCTTTC CCSSCSSCC CTTTTHHHHHHHHTTTCC CCCCCCTTCC CCCCSSCSCCGGGCC CCCGGGGSCCCCCCTTTSCSSSSSSSSCCCCSCC CCCCCCCCCCCCC CCCCCCCSCC CCSCTTSCCCCC CCBTTBSSCC CCCCSCCCCSSCSSSSCC CCSCCCSSSSCCCCCCCCCC CCCCCCGGGTTSSSCSCC CCCBTTBCCCCSSCCCCCCCSSCCCCC CCSCCSSCCCSSSCC CCCCCCCTHHHHHHHHHSCC CHHHHHHHHHHHHHHHHHHHCCTHHHHCCTTHHHHHTTCCHHHHHTTTTHHHHHHHHHHHHHHGGGCC CGGGGTHHHHHHHHHHHC CCHHHHHHHHHHTTTC CHHHHHHHHHHHHHHTCC CCCCTTTTTTSSCSSSSSSSSSSSSCC CTTGGGTTHHHHHHHHHHHHHHHHHHHTTSSHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHSCCSSSTTSCGGGGGGTHHHHHHTTCC

In_Vivo_Mode_of_Delivery

Intranasal Intravenous