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Peptide record

TPDB07781

Antibacterial Anticancer Antifungal Cell-penetrating Peptides Toxicity standard

15 amino acids

3D PDB MODEL

Drag to rotate. Click a residue or atom to inspect it; the selected residue is highlighted in amber.

TPDB ID TPDB07781

Activity

Antibacterial Anticancer Antifungal Cell-penetrating Peptides Toxicity

Activity_category_statistics

Anti-infective peptides Cancer-related peptides Toxicity and safety peptides Delivery and barrier-penetrating peptides

Database_Source

AntiBP3 Peptipedia CancerPPD 2.0 Antifungipept CAFPdb dbAMP CellPPD-MOD CPPsite3.0

Sequence_Type

standard

Has_Modification No

Description A 15-aa standard natural multi-activity (Antibacterial, Anticancer, Antifungal, and other sources) peptide sequence curated from AntiBP3, Peptipedia, CancerPPD 2.0, and other sources, with an available 3D structural model.

Sequence

VKRFKKFFRKLKKSV

FASTA

>TPDB07781|Antibacterial;Anticancer;Antifungal;Cell-penetrating Peptides;Toxicity|length=15
VKRFKKFFRKLKKSV

Formula C94H159N27O17

Absent Amino Acids ANDCEQGHIMPTWY

Common Amino Acids K

Mass 1939.47

PI 12.46

Basic Residues 8

Acidic Residues 0

Hydrophobic Residues 6

Net Charge +8

Boman Index 3.88

Hydrophobicity -0.840

Aliphatic Index 64.67

Half Life

Mammalian: 100 hour Yeast: >20 hour E.coli: >10 hour

Extinction Coefficient Cystines 0

Absorbance 280nm 0.00

Polar Residues 1

number

Amino Acid Distribution

A: 0 R: 2 N: 0 D: 0 C: 0 E: 0 Q: 0 G: 0 H: 0 I: 0 L: 1 K: 6 M: 0 F: 3 P: 0 S: 1 T: 0 W: 0 Y: 0 V: 2

Length

Formula

C94H159N27O17

Mass

1939.47

12.46

Net Charge

Boman Index

3.88

Hydrophobicity

-0.840

Evidence 1 Activity

Activity

Activity Endpoint 10% Hemolysis

Detail Source Activity Toxicity

Activity Measurement >320 µg/mL

Toxicity Type Hemolytic Cytotoxic

Toxicity Raw

10% Hemolysis | >320 | µg/mL | target cell: Human erythrocytes

Target

Target Cell Human erythrocytes

Source & Reference

Database Source NA

Source Database DBAASP

Reference

The antimicrobial peptide ZY4 combats multidrug-resistant Pseudomonas aeruginosa and Acinetobacter baumannii infection. | Proc Natl Acad Sci U S A | 2019

Evidence 2 Activity

Activity

Activity Endpoint 50% Hemolysis

Detail Source Activity Toxicity

Activity Measurement >320 µg/mL

Toxicity Type Hemolytic Cytotoxic

Toxicity Raw

50% Hemolysis | >320 | µg/mL | target cell: Human erythrocytes

Target

Target Cell Human erythrocytes

Source & Reference

Database Source NA

Source Database DBAASP

Reference

The antimicrobial peptide ZY4 combats multidrug-resistant Pseudomonas aeruginosa and Acinetobacter baumannii infection. | Proc Natl Acad Sci U S A | 2019

Evidence 3 Activity

Activity

Activity Endpoint 9.6±4.1% Hemolysis

Detail Source Activity Toxicity

Activity Measurement 320 µg/mL

Toxicity Type Hemolytic Cytotoxic

Toxicity Raw

9.6±4.1% Hemolysis | 320 | µg/mL | target cell: Human erythrocytes

Target

Target Cell Human erythrocytes

Source & Reference

Database Source NA

Source Database DBAASP

Reference

The antimicrobial peptide ZY4 combats multidrug-resistant Pseudomonas aeruginosa and Acinetobacter baumannii infection. | Proc Natl Acad Sci U S A | 2019

Evidence 4 Activity

Activity

Activity Endpoint <5% Hemolysis

Detail Source Activity Toxicity

Activity Measurement 128 µM

Toxicity Type Hemolytic Cytotoxic

Toxicity Raw

<5% Hemolysis | 128 | µM | target cell: Mouse erythrocytes

Target

Target Cell Mouse erythrocytes

Source & Reference

Database Source NA

Source Database DBAASP

Reference

Fatty acid chain modification enhances the serum stability of antimicrobial peptide B1 and activities against Staphylococcus aureus and Klebsiella pneumoniae | Bioorg Chem | 2025

Evidence 5 Activity

Activity

Activity Endpoint 15% Cytotoxicity

Detail Source Activity Toxicity

Activity Measurement 128 µM

Toxicity Type Cytotoxic

Toxicity Raw

15% Cytotoxicity | 128 | µM | target cell: Human embryonic kidney HEK293T cells

Target

Target Cell Human embryonic kidney HEK293T cells

Source & Reference

Database Source NA

Source Database DBAASP

Reference

Fatty acid chain modification enhances the serum stability of antimicrobial peptide B1 and activities against Staphylococcus aureus and Klebsiella pneumoniae | Bioorg Chem | 2025

Evidence 6 Activity

Activity

Activity Endpoint 25% Cytotoxicity

Detail Source Activity Toxicity

Activity Measurement 128 µM

Toxicity Type Cytotoxic

Toxicity Raw

25% Cytotoxicity | 128 | µM | target cell: Human embryonic kidney HEK293T cells

Target

Target Cell Human embryonic kidney HEK293T cells

Source & Reference

Database Source NA

Source Database DBAASP

Reference

Fatty acid chain modification enhances the serum stability of antimicrobial peptide B1 and activities against Staphylococcus aureus and Klebsiella pneumoniae | Bioorg Chem | 2025

Evidence 7 Activity

Activity

Activity Endpoint 40% Cytotoxicity

Detail Source Activity Toxicity

Activity Measurement 128 µM

Toxicity Type Cytotoxic

Toxicity Raw

40% Cytotoxicity | 128 | µM | target cell: Human embryonic kidney HEK293T cells

Target

Target Cell Human embryonic kidney HEK293T cells

Source & Reference

Database Source NA

Source Database DBAASP

Reference

Fatty acid chain modification enhances the serum stability of antimicrobial peptide B1 and activities against Staphylococcus aureus and Klebsiella pneumoniae | Bioorg Chem | 2025

Evidence 8 Activity

Activity

Activity Endpoint IC50

Detail Source Activity Toxicity

Activity Measurement 87.2±9.1 µM

Toxicity Type Cytotoxic

Toxicity Raw

IC50 | 87.2±9.1 | µM | target cell: Human gastric epithelial cells GES-1

Target

Target Cell Human gastric epithelial cells GES-1

Source & Reference

Database Source NA

Source Database DBAASP

Reference

Aliphatic acid-conjugated antimicrobial peptides - potential agents with anti-tumor, multidrug resistance-reversing activity and enhanced stability. | Org Biomol Chem | 2015

Evidence 9 Activity

Activity

Activity Endpoint IC50

Detail Source Activity Toxicity

Activity Measurement 72.7±6.3 µM

Toxicity Type Cytotoxic

Toxicity Raw

IC50 | 72.7±6.3 | µM | target cell: Human embryonic kidney HEK293 cells

Target

Target Cell Human embryonic kidney HEK293 cells

Source & Reference

Database Source NA

Source Database DBAASP

Reference

Aliphatic acid-conjugated antimicrobial peptides - potential agents with anti-tumor, multidrug resistance-reversing activity and enhanced stability. | Org Biomol Chem | 2015

Evidence 10 Activity

Activity

Activity Endpoint 50% Hemolysis

Detail Source Activity Toxicity

Activity Measurement >320 µg/mL

Toxicity Type Hemolytic Cytotoxic

Toxicity Raw

50% Hemolysis | >320 | µg/mL | target cell: Human erythrocytes

Target

Target Cell Human erythrocytes

Source & Reference

Database Source NA

Source Database DBAASP

Reference

Structure-activity relationships of a snake cathelicidin-related peptide, BF-15. | Peptides | 2011

Evidence 11 Activity

Activity

Activity Endpoint 10% Hemolysis

Detail Source Activity Toxicity

Activity Measurement 320 µg/mL

Toxicity Type Hemolytic Cytotoxic

Toxicity Raw

10% Hemolysis | 320 | µg/mL | target cell: Human erythrocytes

Target

Target Cell Human erythrocytes

Source & Reference

Database Source NA

Source Database DBAASP

Reference

Structure-activity relationships of a snake cathelicidin-related peptide, BF-15. | Peptides | 2011

Evidence 12 Activity

Activity

Activity Endpoint IC50

Detail Source Activity Toxicity

Activity Measurement 81.3±6.8 µM

Toxicity Type Cytotoxic

Toxicity Raw

IC50 | 81.3±6.8 | µM | target cell: Human gastric epithelial cells GES-1

Target

Target Cell Human gastric epithelial cells GES-1

Source & Reference

Database Source NA

Source Database DBAASP

Reference

Aliphatic acid-conjugated antimicrobial peptides - potential agents with anti-tumor, multidrug resistance-reversing activity and enhanced stability. | Org Biomol Chem | 2015

Evidence 13 Activity

Activity

Activity Endpoint IC50

Detail Source Activity Toxicity

Activity Measurement 74.1±5.8 µM

Toxicity Type Cytotoxic

Toxicity Raw

IC50 | 74.1±5.8 | µM | target cell: Human embryonic kidney HEK293 cells

Target

Target Cell Human embryonic kidney HEK293 cells

Source & Reference

Database Source NA

Source Database DBAASP

Reference

Aliphatic acid-conjugated antimicrobial peptides - potential agents with anti-tumor, multidrug resistance-reversing activity and enhanced stability. | Org Biomol Chem | 2015

Evidence 14 Activity

Activity

Activity Endpoint <10% Hemolysis

Detail Source Activity Toxicity

Activity Measurement 160 µM

Toxicity Type Hemolytic Cytotoxic

Toxicity Raw

<10% Hemolysis | 160 | µM | target cell: Human erythrocytes

Target

Target Cell Human erythrocytes

Source & Reference

Database Source NA

Source Database DBAASP

Reference

Aliphatic acid-conjugated antimicrobial peptides - potential agents with anti-tumor, multidrug resistance-reversing activity and enhanced stability. | Org Biomol Chem | 2015

Evidence 15 Activity

Activity

Activity Endpoint 32% Hemolysis

Detail Source Activity Toxicity

Activity Measurement 128 µM

Toxicity Type Hemolytic Cytotoxic

Toxicity Raw

32% Hemolysis | 128 | µM | target cell: Mouse erythrocytes

Target

Target Cell Mouse erythrocytes

Source & Reference

Database Source NA

Source Database DBAASP

Reference

Fatty acid chain modification enhances the serum stability of antimicrobial peptide B1 and activities against Staphylococcus aureus and Klebsiella pneumoniae | Bioorg Chem | 2025

Evidence 16 Activity

Activity

Activity Endpoint IC50

Detail Source Activity Toxicity

Activity Measurement 77.6±7.3 µM

Toxicity Type Cytotoxic

Toxicity Raw

IC50 | 77.6±7.3 | µM | target cell: Human gastric epithelial cells GES-1

Target

Target Cell Human gastric epithelial cells GES-1

Source & Reference

Database Source NA

Source Database DBAASP

Reference

Aliphatic acid-conjugated antimicrobial peptides - potential agents with anti-tumor, multidrug resistance-reversing activity and enhanced stability. | Org Biomol Chem | 2015

Evidence 17 Activity

Activity

Activity Endpoint IC50

Detail Source Activity Toxicity

Activity Measurement 75.8±6.6 µM

Toxicity Type Cytotoxic

Toxicity Raw

IC50 | 75.8±6.6 | µM | target cell: Human embryonic kidney HEK293 cells

Target

Target Cell Human embryonic kidney HEK293 cells

Source & Reference

Database Source NA

Source Database DBAASP

Reference

Aliphatic acid-conjugated antimicrobial peptides - potential agents with anti-tumor, multidrug resistance-reversing activity and enhanced stability. | Org Biomol Chem | 2015

Evidence 18 Activity

Activity

Activity Endpoint IC50

Detail Source Activity Toxicity

Activity Measurement 70.8±7.1 µM

Toxicity Type Cytotoxic

Toxicity Raw

IC50 | 70.8±7.1 | µM | target cell: Human gastric epithelial cells GES-1

Target

Target Cell Human gastric epithelial cells GES-1

Source & Reference

Database Source NA

Source Database DBAASP

Reference

Aliphatic acid-conjugated antimicrobial peptides - potential agents with anti-tumor, multidrug resistance-reversing activity and enhanced stability. | Org Biomol Chem | 2015

Evidence 19 Activity

Activity

Activity Endpoint IC50

Detail Source Activity Toxicity

Activity Measurement 70.2±5.0 µM

Toxicity Type Cytotoxic

Toxicity Raw

IC50 | 70.2±5.0 | µM | target cell: Human embryonic kidney HEK293 cells

Target

Target Cell Human embryonic kidney HEK293 cells

Source & Reference

Database Source NA

Source Database DBAASP

Reference

Aliphatic acid-conjugated antimicrobial peptides - potential agents with anti-tumor, multidrug resistance-reversing activity and enhanced stability. | Org Biomol Chem | 2015

Evidence 20 Activity

Activity

Activity Endpoint IC50

Detail Source Activity Toxicity

Activity Measurement 67.6±6.5 µM

Toxicity Type Cytotoxic

Toxicity Raw

IC50 | 67.6±6.5 | µM | target cell: Human gastric epithelial cells GES-1

Target

Target Cell Human gastric epithelial cells GES-1

Source & Reference

Database Source NA

Source Database DBAASP

Reference

Aliphatic acid-conjugated antimicrobial peptides - potential agents with anti-tumor, multidrug resistance-reversing activity and enhanced stability. | Org Biomol Chem | 2015

Evidence 21 Activity

Activity

Activity Endpoint IC50

Detail Source Activity Toxicity

Activity Measurement 68.1±5.3 µM

Toxicity Type Cytotoxic

Toxicity Raw

IC50 | 68.1±5.3 | µM | target cell: Human embryonic kidney HEK293 cells

Target

Target Cell Human embryonic kidney HEK293 cells

Source & Reference

Database Source NA

Source Database DBAASP

Reference

Aliphatic acid-conjugated antimicrobial peptides - potential agents with anti-tumor, multidrug resistance-reversing activity and enhanced stability. | Org Biomol Chem | 2015

Evidence 22 Activity

Activity

Activity Endpoint IC50

Detail Source Activity Toxicity

Activity Measurement 66.1±5.4 µM

Toxicity Type Cytotoxic

Toxicity Raw

IC50 | 66.1±5.4 | µM | target cell: Human gastric epithelial cells GES-1

Target

Target Cell Human gastric epithelial cells GES-1

Source & Reference

Database Source NA

Source Database DBAASP

Reference

Aliphatic acid-conjugated antimicrobial peptides - potential agents with anti-tumor, multidrug resistance-reversing activity and enhanced stability. | Org Biomol Chem | 2015

Evidence 23 Activity

Activity

Activity Endpoint IC50

Detail Source Activity Toxicity

Activity Measurement 66.4±4.8 µM

Toxicity Type Cytotoxic

Toxicity Raw

IC50 | 66.4±4.8 | µM | target cell: Human embryonic kidney HEK293 cells

Target

Target Cell Human embryonic kidney HEK293 cells

Source & Reference

Database Source NA

Source Database DBAASP

Reference

Aliphatic acid-conjugated antimicrobial peptides - potential agents with anti-tumor, multidrug resistance-reversing activity and enhanced stability. | Org Biomol Chem | 2015

Evidence 24 Activity

Activity

Activity Endpoint IC50

Detail Source Activity Toxicity

Activity Measurement 102.3 µM

Toxicity Type Hemolytic Cytotoxic

Toxicity Raw

Hemolytic_activity: Not available | Cytotoxicity: GES-1: IC50=102.3 µM

Target

Target Cell erythrocytes

Source & Reference

Database Source NA

Source Database DRAMP

Reference

Amino Acids. 2017 Sep 49(9):1601-1610.

Evidence 25 Activity

Activity

Activity Endpoint IC50

Detail Source Activity Toxicity

Activity Measurement 81.3

Toxicity Type Hemolytic Cytotoxic

Toxicity Raw

Hemolytic_activity: Human erythrocytes: <20% Hemolysis =160 µM | Cytotoxicity: GES-1: IC50=81.3 ± 6.8 µM HEK293: IC50=81.3 ± 6.8 µM

Target

Target Cell erythrocytes

Source & Reference

Database Source NA

Source Database DRAMP

Reference

Org Biomol Chem. 2015 Jul 28 13(28):7673-80.

Evidence 26 Activity

Activity

Activity Endpoint IC50

Detail Source Activity Toxicity

Activity Measurement 77.6

Toxicity Type Hemolytic Cytotoxic

Toxicity Raw

Hemolytic_activity: Human erythrocytes: <20% Hemolysis =160 µM | Cytotoxicity: GES-1: IC50=77.6 ± 7.3 µM HEK293: IC50=75.8 ± 6.6 µM

Target

Target Cell erythrocytes

Source & Reference

Database Source NA

Source Database DRAMP

Reference

Org Biomol Chem. 2015 Jul 28 13(28):7673-80.

Evidence 27 Activity

Activity

Activity Endpoint IC50

Detail Source Activity Toxicity

Activity Measurement 70.8

Toxicity Type Hemolytic Cytotoxic

Toxicity Raw

Hemolytic_activity: Human erythrocytes: <20% Hemolysis =160 µM | Cytotoxicity: GES-1: IC50=70.8 ± 7.1 µM HEK293: IC50=70.2 ± 5.0 µM

Target

Target Cell erythrocytes

Source & Reference

Database Source NA

Source Database DRAMP

Reference

Org Biomol Chem. 2015 Jul 28 13(28):7673-80.

Evidence 28 Activity

Activity

Activity Endpoint IC50

Detail Source Activity Toxicity

Activity Measurement 67.6

Toxicity Type Hemolytic Cytotoxic

Toxicity Raw

Hemolytic_activity: Human erythrocytes: <20% Hemolysis =160 µM | Cytotoxicity: GES-1: IC50=67.6 ± 6.5 µM HEK293: IC50=68.1 ± 5.3 µM

Target

Target Cell erythrocytes

Source & Reference

Database Source NA

Source Database DRAMP

Reference

Org Biomol Chem. 2015 Jul 28 13(28):7673-80.

Evidence 29 Activity

Activity

Activity Endpoint IC50

Detail Source Activity Toxicity

Activity Measurement 66.1

Toxicity Type Hemolytic Cytotoxic

Toxicity Raw

Hemolytic_activity: Human erythrocytes: <20% Hemolysis =160 µM | Cytotoxicity: GES-1: IC50=66.1 ± 5.4 µM HEK293: IC50=66.4 ± 4.8 µM

Target

Target Cell erythrocytes

Source & Reference

Database Source NA

Source Database DRAMP

Reference

Org Biomol Chem. 2015 Jul 28 13(28):7673-80.