TPDB

3D PDB MODEL

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TPDB ID TPDB07606

Peptide_Name

KLA DP1

Activity

Antibacterial Anticancer Antifungal Antiparasitic Antiviral Cell-penetrating Peptides Hemolysis Toxicity Tumor-homing Peptides

Activity_category_statistics

Anti-infective peptides Cancer-related peptides Toxicity and safety peptides Delivery and barrier-penetrating peptides

Database_Source

AntiBP3 Peptipedia AntiCP 2.0 CancerPPD 2.0 SATPdb dbACP iACP-DRLF pep-lab Antifungipept CAFPdb dbAMP CPPsite3.0 PerseuCPP APD_complete PD_Hemolysis TumorHoPe2.0

Sequence_Type

standard

Has_Modification No

Description A 14-aa standard natural multi-activity (Antibacterial, Anticancer, Antifungal, and other sources) peptide sequence curated from AntiBP3, Peptipedia, AntiCP 2.0, and other sources, with an available 3D structural model.

Sequence

KLAKLAKKLAKLAK

FASTA

>TPDB07606|Antibacterial;Anticancer;Antifungal;Antiparasitic;Antiviral;Cell-penetrating Peptides;Hemolysis;Toxicity;Tumor-homing Peptides|length=14
KLAKLAKKLAKLAK

Formula C72H138N20O15

Absent Amino Acids RNDCEQGHIMFPSTWYV

Common Amino Acids K

Mass 1524.01

PI 11.21

Basic Residues 6

Acidic Residues 0

Hydrophobic Residues 8

Net Charge +6

Boman Index 3.02

Hydrophobicity -0.071

Aliphatic Index 140.00

Half Life

Mammalian: 1.3 hour Yeast: 3 min E.coli: 2 min

Extinction Coefficient Cystines 0

Absorbance 280nm 0.00

Polar Residues 0

number

4

A

0

R

0

N

0

D

0

C

0

E

0

Q

0

G

0

H

0

I

4

L

6

K

0

M

0

F

0

P

0

S

0

T

0

W

0

Y

0

V

Amino Acid Distribution

A: 4 R: 0 N: 0 D: 0 C: 0 E: 0 Q: 0 G: 0 H: 0 I: 0 L: 4 K: 6 M: 0 F: 0 P: 0 S: 0 T: 0 W: 0 Y: 0 V: 0

Length

14

Formula

C72H138N20O15

Mass

1524.01

PI

11.21

Net Charge

+6

Boman Index

3.02

Hydrophobicity

-0.071

C_terminal_Modification

Free

Chirality

L

Ionic_State

Amphipathic

Linear_or_Cyclic

Linear

Linearity

Linear

N_terminal_Modification

Free

Nature

Antimicrobial

Origin

Protein transduction domain

SMILES

N[C@@H](CCCCN)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](C)C(=O)N[C@@H](CCCCN)C(=O)O

Evidence 1 Activity

Activity

Activity Endpoint LC50

Activity Raw

LC50 =< 50 µM

Detail Source Activity Anticancer

Activity Measurement <50 µM

Target

Cancer Type Renal Cancer

Cell Line MCA205

Tissue Affected Renal

Assay & Model

Assay MTT/MTS assay

Testing Time 168-h

Source & Reference

Database Source CancerPPD 2.0 dbACP SATPdb

Source CancerPPD2

Year 2001

Other

Source Activity Label	Source Definition
Anticancer	CancerPPD 2.0 manually curated experimentally verified anticancer peptide/protein annotation.

Evidence 2 Activity

Activity

Detail Source Activity Cell-penetrating Peptides

Target

Cell Line Beas-2B, A549, Caco-2 and AGS

Assay & Model

In Vitro Model Female Sprague-Dawley rats

In Vivo Model Female Sprague-Dawley rats

Source & Reference

Database Source CPPsite3.0

Source CPPsite3

Other

Source Activity Label	Source Definition
Cell-penetrating Peptides	CPPsite3.0 experimentally validated cell-penetrating peptide annotation.

CPP Category Natural residues Linear L Amphipathic

Source of Peptide Synthetic

Cargo Type Fluorophore (FITC)

In Vitro or In Vivo In vitro and in vivo

DSSP Secondary Structure CTHHHHHHHTTTCC

Evidence 3 Activity

Activity

Activity Endpoint 10% Hemolysis

Detail Source Activity Toxicity

Activity Measurement 64 µM

Toxicity Type Hemolytic Cytotoxic

Toxicity Raw

10% Hemolysis | 64 | µM | target cell: Human erythrocytes

Target

Target Cell Human erythrocytes

Source & Reference

Database Source NA

Source Database DBAASP

Reference

Improved bioactivity of antimicrobial peptides by addition of amino-terminal copper and nickel (ATCUN) binding motifs. | ChemMedChem | 2014

Evidence 4 Activity

Activity

Activity Endpoint <10% Hemolysis

Detail Source Activity Toxicity

Activity Measurement >750 µM

Toxicity Type Hemolytic Cytotoxic

Toxicity Raw

<10% Hemolysis | >750 | µM | target cell: Human erythrocytes

Target

Target Cell Human erythrocytes

Source & Reference

Database Source NA

Source Database DBAASP

Reference

De novo antimicrobial peptides with low mammalian cell toxicity. | J Med Chem | 1996

Evidence 5 Activity

Activity

Activity Endpoint 99% Killing

Detail Source Activity Toxicity

Activity Measurement >517 µM

Toxicity Type Cytotoxic

Toxicity Raw

99% Killing | >517 | µM | target cell: Mouse fibroblasts NIH 3T3

Target

Target Cell Mouse fibroblasts NIH 3T3

Source & Reference

Database Source NA

Source Database DBAASP

Reference

De novo antimicrobial peptides with low mammalian cell toxicity. | J Med Chem | 1996

Evidence 6 Activity

Activity

Detail Source Activity Tumor-homing Peptides

Target

Target Cell Tumor cell, Tumor vessel, Tumor stem cell, Endothelial cell,

Homing or Targeting Homing

Receptors Biomarker gC1q/p32 receptor

Source & Reference

Database Source TumorHoPe2.0

Source TumorHoPe2

Title Targeted delivery system and methods of use therefor

Year 2022

Reference

US 11512110 B2

Patent US 11512110 B2

Other

Source Activity Label	Source Definition
Tumor-homing Peptides	TumorHoPe2.0 experimentally validated tumor-homing peptide annotation.

InVivo Model Athymic nude mice, ApoE null mice

Source Type Phage display library

Phage Display Library T7 phage / cyclic Cx7C and linear X7 peptide library

Conjugate Payload Nanoworms(NWs), polyethylene glycol

Evidence 7 Hemolysis

Source & Reference

source APD_complete PD_Hemolysis

Database Source APD_complete PD_Hemolysis

Other

Source Activity Label	Source Definition
Hemolysis	APD_complete APD-derived hemolytic peptide annotation.

Conjugate_Payload

Nanoworms(NWs), polyethylene glycol

DSSP_Secondary_Structure

CTHHHHHHHTTTCC

InVivo_Model

Athymic nude mice, ApoE null mice

Phage_Display_Library

T7 phage / cyclic Cx7C and linear X7 peptide library