Back to search

Peptide record

TPDB07454

buCATHL4B(4B) Antibacterial Antifungal Cell-penetrating Peptides Hemolysis Toxicity Tumor-homing Peptides standard
14 amino acids
Basic Information
3D PDB MODEL
Drag to rotate. Click a residue or atom to inspect it; the selected residue is highlighted in amber.
TPDB07454
buCATHL4B(4B)
Antibacterial Antifungal Cell-penetrating Peptides Hemolysis Toxicity Tumor-homing Peptides
Anti-infective peptides Cancer-related peptides Toxicity and safety peptides Delivery and barrier-penetrating peptides
AntiBP3 Peptipedia Antifungipept CPPsite3.0 APD_complete PD_Hemolysis ToxinPred 3.0 StackTHPred TumorHoPe2.0
standard
No
A 14-aa standard natural multi-activity (Antibacterial, Antifungal, Cell-penetrating Peptides, and other sources) peptide sequence curated from AntiBP3, Peptipedia, Antifungipept, and other sources, with an available 3D structural model.
Sequence
AIPWIWIWRLLRKG
Physicochemical Analysis
C91H138N24O15
NDCEQHMFSTYV
IW
1808.25
12.41
3
0
9
+3
-4.70
0.379
146.43
Mammalian: 4.4 hour Yeast: >20 hour E.coli: >10 hour
16500
912.49
0
Residue Composition
number
1
A
2
R
0
N
0
D
0
C
0
E
0
Q
1
G
0
H
3
I
2
L
1
K
0
M
0
F
1
P
0
S
0
T
3
W
0
Y
0
V
Amino Acid Distribution
A: 1 R: 2 N: 0 D: 0 C: 0 E: 0 Q: 0 G: 1 H: 0 I: 3 L: 2 K: 1 M: 0 F: 0 P: 1 S: 0 T: 0 W: 3 Y: 0 V: 0
Chemical Descriptors
14
C91H138N24O15
1808.25
12.41
+3
-4.70
0.379
Free
L
Linear
Free
N[C@@H](C)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N1CCC[C@H]1C(=O)N[C@@H](CC1=CN=C(C)/C/1=C\C)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H](CC1=C(C(=NC1)C)CC)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H](CC1=CN=C(C)/C/1=C\C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCCN)C(=O)NCC(=O)O
Evidence Records 8 records
Evidence 1 Activity

Activity

Cell-penetrating Peptides

Target

HeLa Cells

Source & Reference

CPPsite3.0
CPPsite3

Other

Source Activity Label Source Definition
Cell-penetrating Peptides
CPPsite3.0 experimentally validated cell-penetrating peptide annotation.
Natural residues Linear L
Protein derived
EGFP protein
Cytoplasm
EGFP‐CAT 2‐8‐times higher intensity than other derivative and 3‐6 times higher than EGFP‐LL37, EGFP‐IL, EGFP‐L‐K6, and EGFP‐TAT
In vitro
CCCCCHHHHHHTCC
Evidence 2 Activity

Activity

15% Hemolysis
Toxicity
7.5 µM
Hemolytic Cytotoxic
15% Hemolysis | 7.5 | µM | target cell: Sheep erythrocytes

Target

Sheep erythrocytes

Source & Reference

NA
DBAASP
Beneficial Impacts of Incorporating the Non-Natural Amino Acid Azulenyl-Alanine into the Trp-Rich Antimicrobial Peptide buCATHL4B. | Biomolecules | 2021
Evidence 3 Activity

Activity

34% Hemolysis
Toxicity
15 µM
Hemolytic Cytotoxic
34% Hemolysis | 15 | µM | target cell: Sheep erythrocytes

Target

Sheep erythrocytes

Source & Reference

NA
DBAASP
Beneficial Impacts of Incorporating the Non-Natural Amino Acid Azulenyl-Alanine into the Trp-Rich Antimicrobial Peptide buCATHL4B. | Biomolecules | 2021
Evidence 4 Activity

Activity

95% Killing
Toxicity
50 µM
Cytotoxic
95% Killing | 50 | µM | target cell: Mouse fibroblasts NIH 3T3

Target

Mouse fibroblasts NIH 3T3

Source & Reference

NA
DBAASP
Beneficial Impacts of Incorporating the Non-Natural Amino Acid Azulenyl-Alanine into the Trp-Rich Antimicrobial Peptide buCATHL4B. | Biomolecules | 2021
Evidence 5 Activity

Activity

55% Hemolysis
Toxicity
150 µM
Hemolytic Cytotoxic
55% Hemolysis | 150 | µM | target cell: Buffalo erythrocytes

Target

Buffalo erythrocytes

Source & Reference

NA
DBAASP
Diversity, Antimicrobial Action and Structure-Activity Relationship of Buffalo Cathelicidins. | PLoS One | 2015
Evidence 6 Activity

Activity

100% Hemolysis
Toxicity
15 µM
Hemolytic Cytotoxic
100% Hemolysis | 15 | µM | target cell: Human erythrocytes

Target

Human erythrocytes

Source & Reference

NA
DBAASP
Investigation of the Role of Aromatic Residues in the Antimicrobial Peptide BuCATHL4B. | Protein Pept Lett | 2021
Evidence 7 Activity

Activity

Tumor-homing Peptides

Target

Tumor cell
Homing
Liver cancer, Cervical cancer

Source & Reference

TumorHoPe2.0
TumorHoPe2
Screening and characterization of a novel high-efficiency tumor-homing cell-penetrating peptide from the buffalo cathelicidin family
2019
Xu Y Y et al. J Pept Sci. 2019 Sep 25(9):e3201

Other

Source Activity Label Source Definition
Tumor-homing Peptides
TumorHoPe2.0 experimentally validated tumor-homing peptide annotation.
Human Cervical Tumor cell line HeLa, Human Hepatoma cell line SMMC‐7721, Human Non‐Small‐Cell Lung Tumor cell line A549, carcinoma cell line NCI‐H460, Human Embryonic Lung cell line MRC‐5
Synthetic
Evidence 8 Hemolysis

Source & Reference

APD_complete PD_Hemolysis
APD_complete PD_Hemolysis

Other

Source Activity Label Source Definition
Hemolysis
APD_complete APD-derived hemolytic peptide annotation.
Additional Detail Fields 2 fields
CCCCCHHHHHHTCC
Human Cervical Tumor cell line HeLa, Human Hepatoma cell line SMMC‐7721, Human Non‐Small‐Cell Lung Tumor cell line A549, carcinoma cell line NCI‐H460, Human Embryonic Lung cell line MRC‐5