TPDB

3D PDB MODEL

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TPDB ID TPDB07372

Activity

Antibacterial Antifungal Hemolysis Toxicity

Activity_category_statistics

Anti-infective peptides Toxicity and safety peptides

Database_Source

AntiBP3 Peptipedia CAFPdb dbAMP PD_Hemolysis

Sequence_Type

standard

Has_Modification No

Description A 13-aa standard natural multi-activity (Antibacterial, Antifungal, Hemolysis, and other sources) peptide sequence curated from AntiBP3, Peptipedia, CAFPdb, and other sources, with an available 3D structural model.

Sequence

FVKWFSKFLGRIL

FASTA

>TPDB07372|Antibacterial;Antifungal;Hemolysis;Toxicity|length=13
FVKWFSKFLGRIL

Formula C84H125N19O15

Absent Amino Acids ANDCEQHMPTY

Common Amino Acids F

Mass 1641.03

PI 11.64

Basic Residues 3

Acidic Residues 0

Hydrophobic Residues 8

Net Charge +3

Boman Index -3.67

Hydrophobicity 0.792

Aliphatic Index 112.31

Half Life

Mammalian: 1.1 hour Yeast: 3 min E.coli: 2 min

Extinction Coefficient Cystines 5500

Absorbance 280nm 335.15

Polar Residues 1

number

0

A

1

R

0

N

0

D

0

C

0

E

0

Q

1

G

0

H

1

I

2

L

2

K

0

M

3

F

0

P

1

S

0

T

1

W

0

Y

1

V

Amino Acid Distribution

A: 0 R: 1 N: 0 D: 0 C: 0 E: 0 Q: 0 G: 1 H: 0 I: 1 L: 2 K: 2 M: 0 F: 3 P: 0 S: 1 T: 0 W: 1 Y: 0 V: 1

Length

13

Formula

C84H125N19O15

Mass

1641.03

PI

11.64

Net Charge

+3

Boman Index

-3.67

Hydrophobicity

0.792

Evidence 1 Activity

Activity

Activity Endpoint 100% Hemolysis

Detail Source Activity Toxicity

Activity Measurement 30 µM

Toxicity Type Hemolytic Cytotoxic

Toxicity Raw

100% Hemolysis | 30 | µM | target cell: Human erythrocytes

Target

Target Cell Human erythrocytes

Source & Reference

Database Source NA

Source Database DBAASP

Reference

A Noncytotoxic Temporin L Analogue with In Vivo Antibacterial and Antiendotoxin Activities and a Nonmembrane-Lytic Mode of Action | ACS Infect Dis | 2020

Evidence 2 Activity

Activity

Activity Endpoint 100% Killing

Detail Source Activity Toxicity

Activity Measurement 30 µM

Toxicity Type Cytotoxic

Toxicity Raw

100% Killing | 30 | µM | target cell: Mouse fibroblasts NIH 3T3

Target

Target Cell Mouse fibroblasts NIH 3T3

Source & Reference

Database Source NA

Source Database DBAASP

Reference

A Noncytotoxic Temporin L Analogue with In Vivo Antibacterial and Antiendotoxin Activities and a Nonmembrane-Lytic Mode of Action | ACS Infect Dis | 2020

Evidence 3 Activity

Activity

Activity Endpoint 80% Killing

Detail Source Activity Toxicity

Activity Measurement 10 µM

Toxicity Type Cytotoxic

Toxicity Raw

80% Killing | 10 | µM | target cell: Mouse fibroblasts NIH 3T3

Target

Target Cell Mouse fibroblasts NIH 3T3

Source & Reference

Database Source NA

Source Database DBAASP

Reference

A Noncytotoxic Temporin L Analogue with In Vivo Antibacterial and Antiendotoxin Activities and a Nonmembrane-Lytic Mode of Action | ACS Infect Dis | 2020

Evidence 4 Hemolysis

Source & Reference

source PD_Hemolysis

Database Source PD_Hemolysis