TPDB

3D PDB MODEL

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TPDB ID TPDB07286

Activity

Antibacterial Hemolysis Toxicity

Activity_category_statistics

Anti-infective peptides Toxicity and safety peptides

Database_Source

AntiBP3 Peptipedia HemoPI Hemolytic2 PD_Hemolysis

Sequence_Type

standard

Has_Modification No

Description A 13-aa standard natural multi-activity (Antibacterial, Hemolysis, and Toxicity) peptide sequence curated from AntiBP3, Peptipedia, HemoPI, and other sources, with an available 3D structural model.

Sequence

ILPWKWPLWPWRR

FASTA

>TPDB07286|Antibacterial;Hemolysis;Toxicity|length=13
ILPWKWPLWPWRR

Formula C95H132N24O14

Absent Amino Acids ANDCEQGHMFSTYV

Common Amino Acids W

Mass 1834.25

PI 12.41

Basic Residues 3

Acidic Residues 0

Hydrophobic Residues 7

Net Charge +3

Boman Index -4.87

Hydrophobicity -0.708

Aliphatic Index 90.00

Half Life

Mammalian: 20 hour Yeast: 30 min E.coli: >10 hour

Extinction Coefficient Cystines 22000

Absorbance 280nm 1199.40

Polar Residues 0

number

0

A

2

R

0

N

0

D

0

C

0

E

0

Q

0

G

0

H

1

I

2

L

1

K

0

M

0

F

3

P

0

S

0

T

4

W

0

Y

0

V

Amino Acid Distribution

A: 0 R: 2 N: 0 D: 0 C: 0 E: 0 Q: 0 G: 0 H: 0 I: 1 L: 2 K: 1 M: 0 F: 0 P: 3 S: 0 T: 0 W: 4 Y: 0 V: 0

Length

13

Formula

C95H132N24O14

Mass

1834.25

PI

12.41

Net Charge

+3

Boman Index

-4.87

Hydrophobicity

-0.708

Evidence 1 Activity

Activity

Activity Endpoint MIC

Detail Source Activity Antibacterial

Activity Measurement 3.13 µg/mL

Target

Target Organism B. subtilis IFO 3513

Source & Reference

Database Source NA

Other

Evidence Text

Active against Gram+ B. subtilis IFO 3513 (MIC 3.13 ug/ml), S. aureus IFO 12732 (MIC 1.56 ug/ml), and Gram- E. coli IFO 12734 (MIC 12.5 ug/ml).

Evidence 2 Activity

Activity

Activity Endpoint MIC

Detail Source Activity Antibacterial

Activity Measurement 1.56 µg/mL

Target

Target Organism Staphylococcus aureus IFO 12732

Source & Reference

Database Source NA

Other

Evidence Text

Active against Gram+ B. subtilis IFO 3513 (MIC 3.13 ug/ml), S. aureus IFO 12732 (MIC 1.56 ug/ml), and Gram- E. coli IFO 12734 (MIC 12.5 ug/ml).

Evidence 3 Activity

Activity

Activity Endpoint MIC

Detail Source Activity Antibacterial

Activity Measurement 12.5 µg/mL

Target

Target Organism Escherichia coli IFO 12734

Source & Reference

Database Source NA

Other

Evidence Text

m+ B. subtilis IFO 3513 (MIC 3.13 ug/ml), S. aureus IFO 12732 (MIC 1.56 ug/ml), and Gram- E. coli IFO 12734 (MIC 12.5 ug/ml).

Evidence 4 Activity

Activity

Activity Endpoint 63% Hemolysis

Detail Source Activity Toxicity

Activity Measurement 50 µM

Toxicity Type Hemolytic Cytotoxic

Toxicity Raw

63% Hemolysis | 50 | µM | target cell: Human erythrocytes

Target

Target Cell Human erythrocytes

Source & Reference

Database Source NA

Source Database DBAASP

Reference

Structure-activity relationship of indolicidin, a Trp-rich antibacterial peptide. | J Pept Sci | 2010

Evidence 5 Hemolysis

Source & Reference

source HemoPI PD_Hemolysis

Database Source PD_Hemolysis